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Soybean seeds expressing feedback-insensitive cystathionine γ-synthase exhibit a higher content of methionine.

Abstract：

:Soybean seeds provide an excellent source of protein for human and livestock nutrition. However, their nutritional quality is hampered by a low concentration of the essential sulfur amino acid, methionine (Met). In order to study factors that regulate Met synthesis in soybean seeds, this study used the Met-insensitive form of Arabidopsis cystathionine γ-synthase (AtD-CGS), which is the first committed enzyme of Met biosynthesis. This gene was expressed under the control of a seed-specific promoter, legumin B4, and used to transform the soybean cultivar Zigongdongdou (ZD). In three transgenic lines that exhibited the highest expression level of AtD-CGS, the level of soluble Met increased significantly in developing green seeds (3.8-7-fold). These seeds also showed high levels of other amino acids. This phenomenon was more prominent in two transgenic lines, ZD24 and ZD91. The total Met content, which including Met incorporated into proteins, significantly increased in the mature dry seeds of these two transgenic lines by 1.8- and 2.3-fold, respectively. This elevation was accompanied by a higher content of other protein-incorporated amino acids, which led to significantly higher total protein content in the seeds of these two lines. However, in a third transgenic line, ZD01, the level of total Met and the level of other amino acids did not increase significantly in the mature dry seeds. This line also showed no significant change in protein levels. This suggests a positive connection between high Met content and the synthesis of other amino acids that enable the synthesis of more seed proteins.

journal_name

J Exp Bot

journal_title

Journal of experimental botany

authors

Song S,Hou W,Godo I,Wu C,Yu Y,Matityahu I,Hacham Y,Sun S,Han T,Amir R

doi

10.1093/jxb/ert053

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

1917-26

issue

7

eissn

0022-0957

issn

1460-2431

pii

ert053

journal_volume

64

pub_type

杂志文章

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