Proteomes of hard and soft near-isogenic wheat lines reveal that kernel hardness is related to the amplification of a stress response during endosperm development.

Abstract:

:Wheat kernel texture, a major trait determining the end-use quality of wheat flour, is mainly influenced by puroindolines. These small basic proteins display in vitro lipid binding and antimicrobial properties, but their cellular functions during grain development remain unknown. To gain an insight into their biological function, a comparative proteome analysis of two near-isogenic lines (NILs) of bread wheat Triticum aestivum L. cv. Falcon differing in the presence or absence of the puroindoline-a gene (Pina) and kernel hardness, was performed. Proteomes of the two NILs were compared at four developmental stages of the grain for the metabolic albumin/globulin fraction and the Triton-extracted amphiphilic fraction. Proteome variations showed that, during grain development, folding proteins and stress-related proteins were more abundant in the hard line compared with the soft one. These results, taken together with ultrastructural observations showing that the formation of the protein matrix occurred earlier in the hard line, suggested that a stress response, possibly the unfolded protein response, is induced earlier in the hard NIL than in the soft one leading to earlier endosperm cell death. Quantification of the albumin/globulin fraction and amphiphilic proteins at each developmental stage strengthened this hypothesis as a plateau was revealed from the 500 °Cd stage in the hard NIL whereas synthesis continued in the soft one. These results open new avenues concerning the function of puroindolines which could be involved in the storage protein folding machinery, consequently affecting the development of wheat endosperm and the formation of the protein matrix.

journal_name

J Exp Bot

authors

Lesage VS,Merlino M,Chambon C,Bouchet B,Marion D,Branlard G

doi

10.1093/jxb/err330

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

1001-11

issue

2

eissn

0022-0957

issn

1460-2431

pii

err330

journal_volume

63

pub_type

杂志文章
  • Photosynthetic limitations in two Antarctic vascular plants: importance of leaf anatomical traits and Rubisco kinetic parameters.

    abstract::Particular physiological traits allow the vascular plants Deschampsia antarctica Desv. and Colobanthus quitensis (Kunth) Bartl. to inhabit Antarctica. The photosynthetic performance of these species was evaluated in situ, focusing on diffusive and biochemical constraints to CO2 assimilation. Leaf gas exchange, Chl a f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx148

    authors: Sáez PL,Bravo LA,Cavieres LA,Vallejos V,Sanhueza C,Font-Carrascosa M,Gil-Pelegrín E,Javier Peguero-Pina J,Galmés J

    更新日期:2017-05-17 00:00:00

  • Dwarf alleles differentially affect barley root traits influencing nitrogen acquisition under low nutrient supply.

    abstract::Sustainable food production depends critically on the development of crop genotypes that exhibit high yield under reduced nutrient inputs. Rooting traits have been widely advocated as being able to influence optimal plant performance, while breeding-based improvements in yield of spring barley suggest that this specie...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err089

    authors: Karley AJ,Valentine TA,Squire GR

    更新日期:2011-07-01 00:00:00

  • The family of Peps and their precursors in Arabidopsis: differential expression and localization but similar induction of pattern-triggered immune responses.

    abstract::In Arabidopsis thaliana, the endogenous danger peptides, AtPeps, have been associated with plant defences reminiscent of those induced in pattern-triggered immunity. AtPeps are perceived by two homologous receptor kinases, PEPR1 and PEPR2, and are encoded in the C termini of the PROPEP precursors. Here, we report that...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert330

    authors: Bartels S,Lori M,Mbengue M,van Verk M,Klauser D,Hander T,Böni R,Robatzek S,Boller T

    更新日期:2013-12-01 00:00:00

  • TRV-GFP: a modified Tobacco rattle virus vector for efficient and visualizable analysis of gene function.

    abstract::Virus-induced gene silencing (VIGS) is a useful tool for functional characterization of genes in plants. Unfortunately, the efficiency of infection by Tobacco rattle virus (TRV) is relatively low for some non-Solanaceae plants, which are economically important, such as rose (Rosa sp.). Here, to generate an easy tracea...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert381

    authors: Tian J,Pei H,Zhang S,Chen J,Chen W,Yang R,Meng Y,You J,Gao J,Ma N

    更新日期:2014-01-01 00:00:00

  • Cloning and characterization of a 2-Cys peroxiredoxin from Pisum sativum.

    abstract::A cDNA sequence coding for a pea (Pisum sativum L.) 2-Cys peroxiredoxin (2-Cys Prx) has been cloned. The deduced amino acid sequence showed a high sequence homology to the 2-Cys Prx enzymes of Phaseolus vulgaris (86%), Arabidopsis thaliana (75%), and Spinacia oleracea (75%), and contained a chloroplast target sequence...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh238

    authors: Bernier-Villamor L,Navarro E,Sevilla F,Lázaro JJ

    更新日期:2004-10-01 00:00:00

  • Hyperosmotic stress-induced actin filament reorganization in leaf cells of Chlorophyton comosum.

    abstract::Actin filament (AF) organization was studied during the plasmolytic cycle in leaf cells of Chlorophyton comosum Thunb. In most cells the hyperosmotic treatment induced convex or concave plasmolysis and intense reorganization of the AF cytoskeleton. Thin cortical AFs disappeared and numerous cortical, subcortical and e...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf018

    authors: Komis G,Apostolakos P,Galatis B

    更新日期:2002-08-01 00:00:00

  • Modulation of nitrate reductase: some new insights, an unusual case and a potentially important side reaction.

    abstract::The mechanism of the post-translational modulation of nitrate reductase activity (NR, EC 1.6.6.1) is briefly summarized, and it is shown that by this mechanism nitric oxide production through NR is also rapidly modulated. New and partly unexpected details on the modulation mechanism have been obtained by using immunol...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jexbot/53.370.875

    authors: Kaiser WM,Weiner H,Kandlbinder A,Tsai CB,Rockel P,Sonoda M,Planchet E

    更新日期:2002-04-01 00:00:00

  • Overexpression of SIPK in tobacco enhances ozone-induced ethylene formation and blocks ozone-induced SA accumulation.

    abstract::Ozone induces rapid activation of SIPK, a mitogen-activated protein kinase (MAPK) in tobacco. Through transgenic manipulation it has previously been shown that overexpression of SIPK leads to enhanced ozone-induced lesion formation with concomitant accumulation of ROS. In spite of this hypersensitive phenotype, the ef...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri219

    authors: Samuel MA,Walia A,Mansfield SD,Ellis BE

    更新日期:2005-08-01 00:00:00

  • Soil compaction and the architectural plasticity of root systems.

    abstract::Soil compaction is a serious global problem, and is a major cause of inadequate rooting and poor yield in crops around the world. Root system architecture (RSA) describes the spatial arrangement of root components within the soil and determines the plant's exploration of the soil. Soil strength restricts root growth a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz383

    authors: Correa J,Postma JA,Watt M,Wojciechowski T

    更新日期:2019-11-18 00:00:00

  • The Arabidopsis glutamate receptor-like gene GLR3.6 controls root development by repressing the Kip-related protein gene KRP4.

    abstract::In Arabidopsis, 20 genes encode putative glutamate receptor-like proteins (AtGLRs). However, the functions of most genes are unknown. In this study, our results revealed that loss of function of AtGLR3.6 (atglr3.6-1) leads to reduced primary root growth and fewer lateral roots, whereas AtGLR3.6 overexpression induced ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv576

    authors: Singh SK,Chien CT,Chang IF

    更新日期:2016-03-01 00:00:00

  • Chilling root temperature causes rapid ultrastructural changes in cortical cells of cucumber (Cucumis sativus L.) root tips.

    abstract::Examination of root tips from cucumber (Cucumis sativus L.) seedlings grown at 8 degrees C for varying periods ranging from 15 min to 96 h, showed marked changes in the ultrastructure of cortical cells within only 15 min of exposure. Greater parts of the cortex were affected with longer periods of exposure, but the se...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf071

    authors: Lee SH,Singh AP,Chung GC,Kim YS,Kong IB

    更新日期:2002-11-01 00:00:00

  • Sugar regulation of SUGAR TRANSPORTER PROTEIN 1 (STP1) expression in Arabidopsis thaliana.

    abstract::Sugars regulate the expression of many genes at the transcriptional level. In Arabidopsis thaliana, sugars induce or repress the expression of >1800 genes, including the STP1 (SUGAR TRANSPORTER PROTEIN 1) gene, which encodes an H(+)/monosaccharide cotransporter. STP1 transcript levels decrease more rapidly after the a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru394

    authors: Cordoba E,Aceves-Zamudio DL,Hernández-Bernal AF,Ramos-Vega M,León P

    更新日期:2015-01-01 00:00:00

  • HYL1 is required for establishment of stamen architecture with four microsporangia in Arabidopsis.

    abstract::The stamen produces pollen grains for pollination in higher plants. Coordinated development of four microsporangia in the stamen is essential for normal fertility. The roles of miR165/166-directed pathways in the establishment of adaxial-abaxial polarity have been well defined in leaves. However, the molecular mechani...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert178

    authors: Lian H,Li X,Liu Z,He Y

    更新日期:2013-08-01 00:00:00

  • RootGraph: a graphic optimization tool for automated image analysis of plant roots.

    abstract::This paper outlines a numerical scheme for accurate, detailed, and high-throughput image analysis of plant roots. In contrast to existing root image analysis tools that focus on root system-average traits, a novel, fully automated and robust approach for the detailed characterization of root traits, based on a graph o...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv359

    authors: Cai J,Zeng Z,Connor JN,Huang CY,Melino V,Kumar P,Miklavcic SJ

    更新日期:2015-11-01 00:00:00

  • Activation of ethylene-responsive p-hydroxyphenylpyruvate dioxygenase leads to increased tocopherol levels during ripening in mango.

    abstract::Mango is characterized by high tocopherol and carotenoid content during ripening. From a cDNA screen of differentially expressing genes during mango ripening, a full-length p-hydroxyphenylpyruvate dioxygenase (MiHPPD) gene homologue was isolated that encodes a key enzyme in the biosynthesis of tocopherols. The gene en...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err006

    authors: Singh RK,Ali SA,Nath P,Sane VA

    更新日期:2011-06-01 00:00:00

  • Inefficient double-strand DNA break repair is associated with increased fasciation in Arabidopsis BRCA2 mutants.

    abstract::BRCA2 is a breast tumour susceptibility factor with functions in maintaining genome stability through ensuring efficient double-strand DNA break (DSB) repair via homologous recombination. Although best known in vertebrates, fungi, and higher plants also possess BRCA2-like genes. To investigate the role of Arabidopsis ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp135

    authors: Abe K,Osakabe K,Ishikawa Y,Tagiri A,Yamanouchi H,Takyuu T,Yoshioka T,Ito T,Kobayashi M,Shinozaki K,Ichikawa H,Toki S

    更新日期:2009-01-01 00:00:00

  • Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction.

    abstract::Although the mechanisms of nodule N(2) fixation in legumes are now well documented, some uncertainty remains on the metabolic consequences of water deficit. In most cases, little consideration is given to other organs and, therefore, the coordinated changes in metabolism in leaves, roots, and nodules are not well know...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers367

    authors: Aranjuelo I,Tcherkez G,Molero G,Gilard F,Avice JC,Nogués S

    更新日期:2013-02-01 00:00:00

  • The mitochondrial pentatricopeptide repeat protein EMP12 is involved in the splicing of three nad2 introns and seed development in maize.

    abstract::Plant mitochondrial genes contain cis- and trans-group II introns that must be spliced before translation. The mechanism by which these introns are spliced is not well understood. Several families of proteins have been implicated in the intron splicing, of which the pentatricopeptide repeat (PPR) proteins are proposed...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery432

    authors: Sun F,Xiu Z,Jiang R,Liu Y,Zhang X,Yang YZ,Li X,Zhang X,Wang Y,Tan BC

    更新日期:2019-02-05 00:00:00

  • Temperature during the day, but not during the night, controls flowering of Phalaenopsis orchids.

    abstract::Phalaenopsis orchids are among the most valuable potted flowering crops commercially produced throughout the world because of their long flower life and ease of crop scheduling to meet specific market dates. During commercial production, Phalaenopsis are usually grown at an air temperature > or =28 degrees C to inhibi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl176

    authors: Blanchard MG,Runkle ES

    更新日期:2006-01-01 00:00:00

  • Calcium deprivation disrupts enlargement of Chara corallina cells: further evidence for the calcium pectate cycle.

    abstract::Pectin is a normal constituent of cell walls of green plants. When supplied externally to live cells or walls isolated from the large-celled green alga Chara corallina, pectin removes calcium from load-bearing cross-links in the wall, loosening the structure and allowing it to deform more rapidly under the action of t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers089

    authors: Proseus TE,Boyer JS

    更新日期:2012-06-01 00:00:00

  • Overexpression of HvAKT1 improves drought tolerance in barley by regulating root ion homeostasis and ROS and NO signaling.

    abstract::Potassium (K+) is the major cationic inorganic nutrient utilized for osmotic regulation, cell growth, and enzyme activation in plants. Inwardly rectifying K+ channel 1 (AKT1) is the primary channel for root K+ uptake in plants, but the function of HvAKT1 in barley plants under drought stress has not been fully elucida...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa354

    authors: Feng X,Liu W,Cao F,Wang Y,Zhang G,Chen ZH,Wu F

    更新日期:2020-10-22 00:00:00

  • Zygnema circumcarinatum UTEX 1559 chloroplast and mitochondrial genomes provide insight into land plant evolution.

    abstract::The complete chloroplast and mitochondrial genomes of Charophyta have shed new light on land plant terrestrialization. Here, we report the organellar genomes of the Zygnema circumcarinatum strain UTEX 1559, and a comparative genomics investigation of 33 plastomes and 18 mitogenomes of Chlorophyta, Charophyta (includin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa149

    authors: Orton LM,Fitzek E,Feng X,Grayburn WS,Mower JP,Liu K,Zhang C,Duvall MR,Yin Y

    更新日期:2020-06-11 00:00:00

  • Expression of a cyanobacterial sucrose-phosphate synthase from Synechocystis sp. PCC 6803 in transgenic plants.

    abstract::Sucrose-phosphate synthase (SPS) from the cyanobacterium Synechocystis sp. PCC 6803 lacks all of the Ser residues known to be involved in the regulation of higher plant SPS by protein phosphorylation. The Synechocystis SPS is also not allosterically regulated by glucose 6-phosphate or orthophosphate. To investigate th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg023

    authors: Lunn JE,Gillespie VJ,Furbank RT

    更新日期:2003-01-01 00:00:00

  • Tomato SlDREB gene restricts leaf expansion and internode elongation by downregulating key genes for gibberellin biosynthesis.

    abstract::Plants have evolved and adapted to different environments. Dwarfism is an adaptive trait of plants that helps them avoid high-energy costs under unfavourable conditions. The role of gibberellin (GA) in plant development has been well established. Several plant dehydration-responsive element-binding proteins (DREBs) ha...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers295

    authors: Li J,Sima W,Ouyang B,Wang T,Ziaf K,Luo Z,Liu L,Li H,Chen M,Huang Y,Feng Y,Hao Y,Ye Z

    更新日期:2012-11-01 00:00:00

  • A network perspective on nitrogen metabolism from model to crop plants using integrated 'omics' approaches.

    abstract::Nitrogen (N), as an essential element in amino acids, nucleotides, and proteins, is a key factor in plant growth and development. Omics approaches such as metabolomics and transcriptomics have become a promising way to inspect complex network interactions in N metabolism and can be used for monitoring the uptake and r...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/eru322

    authors: Fukushima A,Kusano M

    更新日期:2014-10-01 00:00:00

  • Calculated activity of Mn2+ at the outer surface of the root cell plasma membrane governs Mn nutrition of cowpea seedlings.

    abstract::Manganese (Mn) is an essential micronutrient for plant growth but is often toxic in acid or waterlogged soils. Using cowpea (Vigna unguiculata L. Walp.) grown with 0.05-1500 μM Mn in solution, two short-term (48 h) solution culture experiments examined if the effects of cations (Ca, Mg, Na, Al, or H) on Mn nutrition a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err097

    authors: Kopittke PM,Blamey FP,Wang P,Menzies NW

    更新日期:2011-07-01 00:00:00

  • Distribution, biological activities, metabolism, and the conceivable function of cis-zeatin-type cytokinins in plants.

    abstract::Cytokinins (CKs) are plant hormones affecting numerous developmental processes. Zeatin and its derivatives are the most important group of isoprenoid CKs. Zeatin occurs as two isomers: while trans-zeatin (transZ) was found to be a bioactive substance, cis-zeatin (cisZ) was reported to have a weak biological impact. Ev...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq457

    authors: Gajdosová S,Spíchal L,Kamínek M,Hoyerová K,Novák O,Dobrev PI,Galuszka P,Klíma P,Gaudinová A,Zizková E,Hanus J,Dancák M,Trávnícek B,Pesek B,Krupicka M,Vanková R,Strnad M,Motyka V

    更新日期:2011-05-01 00:00:00

  • Antisense-mediated suppression of C-hordein biosynthesis in the barley grain results in correlated changes in the transcriptome, protein profile, and amino acid composition.

    abstract::Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the curre...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm254

    authors: Hansen M,Lange M,Friis C,Dionisio G,Holm PB,Vincze E

    更新日期:2007-01-01 00:00:00

  • Iron in Plant-pathogen Interactions.

    abstract::Iron is an essential element for most organisms. As an indispensable co-factor of many enzymes, iron is involved in various crucial metabolic processes, which are required for the survival of plants and pathogens. Conversely, excessive iron produces highly active reactive oxygen species (ROS) that is toxic to the cell...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa516

    authors: Liu Y,Kong D,Wu HL,Ling HQ

    更新日期:2020-11-08 00:00:00

  • Contrasting adaptive strategies to terminal drought-stress gradients in Mediterranean legumes: phenology, productivity, and water relations in wild and domesticated Lupinus luteus L.

    abstract::Our understanding of within-species annual plant adaptation to rainfall gradients is fragmented. Broad-scale ecological applications of Grime's C-S-R triangle are often superficial, while detailed drought physiology tends to be narrow, focusing on elite cultivars. The former lack the detail to explain how plants respo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru006

    authors: Berger JD,Ludwig C

    更新日期:2014-11-01 00:00:00