Abstract:
:The extracellular matrix (ECM) of many charophytes, the assemblage of green algae that are the sister group to land plants, is complex, produced in large amounts, and has multiple essential functions. An extensive secretory apparatus and endomembrane system are presumably needed to synthesize and secrete the ECM, but structural details of such a system have not been fully characterized. Penium margaritaceum is a valuable unicellular model charophyte for studying secretion dynamics. We report that Penium has a highly organized endomembrane system, consisting of 150-200 non-mobile Golgi bodies that process and package ECM components into different sets of vesicles that traffic to the cortical cytoplasm, where they are transported around the cell by cytoplasmic streaming. At either fixed or transient areas, specific cytoplasmic vesicles fuse with the plasma membrane and secrete their constituents. Extracellular polysaccharide (EPS) production was observed to occur in one location of the Golgi body and sometimes in unique Golgi hybrids. Treatment of cells with brefeldin A caused disruption of the Golgi body, and inhibition of EPS secretion and cell wall expansion. The structure of the endomembrane system in Penium provides mechanistic insights into how extant charophytes generate large quantities of ECM, which in their ancestors facilitated the colonization of land.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Domozych DS,Sun L,Palacio-Lopez K,Reed R,Jeon S,Li M,Jiao C,Sørensen I,Fei Z,Rose JKCdoi
10.1093/jxb/eraa039subject
Has Abstractpub_date
2020-06-11 00:00:00pages
3323-3339issue
11eissn
0022-0957issn
1460-2431pii
5715038journal_volume
71pub_type
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