Immunolocalization of FsPK1 correlates this abscisic acid-induced protein kinase with germination arrest in Fagus sylvatica L. seeds.

Abstract:

:An enzymatically active recombinant protein kinase, previously isolated and characterized in Fagus sylvatica L. dormant seeds (FsPK1), was used to obtain a specific polyclonal antibody against this protein. Immunoblotting and immunohistochemical analysis of FsPK1 protein in beech seeds showed a strong immunostaining in the nucleus of the cells located in the vascular tissue of the embryonic axis corresponding to the future apical meristem of the root. This protein kinase was found to accumulate in the seeds only when embryo growth was arrested by application of ABA, while the protein amount decreased during stratification, previously proved to alleviate dormancy, and no protein was detected at all when seed germination was induced by addition of GA(3). These results indicate that FsPK1 may be involved in the control of the embryo growth mediated by ABA and GAs during the transition from dormancy to germination in Fagus sylvatica seeds.

journal_name

J Exp Bot

authors

Reyes D,Rodríguez D,Lorenzo O,Nicolás G,Cañas R,Cantón FR,Canovas FM,Nicolás C

doi

10.1093/jxb/erj077

keywords:

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

923-9

issue

4

eissn

0022-0957

issn

1460-2431

pii

erj077

journal_volume

57

pub_type

杂志文章
  • Long-distance signalling from roots to shoots assessed: the flooding story.

    abstract::Several kinds of signal may be generated when roots are exposed to an environmental stress. Some, but not all, are conveyed to shoots in the transpiration stream. Principles are summarized that may help establish experimentally the presence or intensity of root signals transported by transpiration. In many dryland spe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jexbot/53.367.175

    authors: Jackson MB

    更新日期:2002-02-01 00:00:00

  • Genome-wide analysis reveals dynamic changes in expression of microRNAs during vascular cambium development in Chinese fir, Cunninghamia lanceolata.

    abstract::MicroRNAs (miRNAs) are small noncoding regulatory RNAs that play key roles in the process of plant development. To date, extensive studies of miRNAs have been performed in a few model plants, but few efforts have focused on small RNAs (sRNAs) in conifers because of the lack of reference sequences for their enormous ge...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv103

    authors: Qiu Z,Li X,Zhao Y,Zhang M,Wan Y,Cao D,Lu S,Lin J

    更新日期:2015-06-01 00:00:00

  • Stress-responsive alpha-dioxygenase expression in tomato roots.

    abstract::Alpha-dioxygenase (alpha-DOX) enzymes catalyse the oxygenation of fatty acids to yield a newly identified group of oxylipins that play a role in protecting tissues from oxidative damage and cell death. In tomato (Lycopersicon esculentum Mill.) alpha-DOX was identified as salt-regulated by differential display of mRNA,...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri038

    authors: Tirajoh A,Aung TS,McKay AB,Plant AL

    更新日期:2005-02-01 00:00:00

  • The regulation of MADS-box gene expression during ripening of banana and their regulatory interaction with ethylene.

    abstract::Six MaMADS-box genes have been cloned from the banana fruit cultivar Grand Nain. The similarity of these genes to tomato LeRIN is low and neither MaMADS2 nor MaMADS1 complement the tomato rin mutation. Nevertheless, the expression patterns, specifically in fruit and the induction during ripening and in response to eth...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq017

    authors: Elitzur T,Vrebalov J,Giovannoni JJ,Goldschmidt EE,Friedman H

    更新日期:2010-03-01 00:00:00

  • A correlative microscopy approach relates microtubule behaviour, local organ geometry, and cell growth at the Arabidopsis shoot apical meristem.

    abstract::Cortical microtubules (CMTs) are often aligned in a particular direction in individual cells or even in groups of cells and play a central role in the definition of growth anisotropy. How the CMTs themselves are aligned is not well known, but two hypotheses have been proposed. According to the first hypothesis, CMTs a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert352

    authors: Burian A,Ludynia M,Uyttewaal M,Traas J,Boudaoud A,Hamant O,Kwiatkowska D

    更新日期:2013-12-01 00:00:00

  • Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity.

    abstract::Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis NLRs RRS1-R and RPS4, that confer recognition of bacterial effect...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz571

    authors: Ngou BPM,Ahn HK,Ding P,Redkar A,Brown H,Ma Y,Youles M,Tomlinson L,Jones JDG

    更新日期:2020-03-25 00:00:00

  • Metabolic characterization of loci affecting sensory attributes in tomato allows an assessment of the influence of the levels of primary metabolites and volatile organic contents.

    abstract::Numerous studies have revealed the extent of genetic and phenotypic variation between both species and cultivars of tomato. Using a series of tomato lines resulting from crosses between a cherry tomato and three independent large fruit cultivar (Levovil, VilB, and VilD), extensive profiling of both central primary met...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp086

    authors: Zanor MI,Rambla JL,Chaïb J,Steppa A,Medina A,Granell A,Fernie AR,Causse M

    更新日期:2009-01-01 00:00:00

  • A methylation status analysis of the apomixis-specific region in Paspalum spp. suggests an epigenetic control of parthenogenesis.

    abstract::Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single locus which is blocked in terms of recombination. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin, namely the presence of repetitive elements, gene degeneration, and de-regulation....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru354

    authors: Podio M,Cáceres ME,Samoluk SS,Seijo JG,Pessino SC,Ortiz JP,Pupilli F

    更新日期:2014-12-01 00:00:00

  • Fine mapping of a quantitative trait locus for spikelet number per panicle in a new plant type rice and evaluation of a near-isogenic line for grain productivity.

    abstract::Total spikelet number per panicle (TSN) is one of the determinants of grain productivity in rice (Oryza sativa L.). In this study, we attempted to detect quantitative trait loci (QTLs) for TSN in the introgression lines with high TSN, derived from the cross of Indica Group variety IR 64 with new plant type lines. Two ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx128

    authors: Sasaki K,Fujita D,Koide Y,Lumanglas PD,Gannaban RB,Tagle AG,Obara M,Fukuta Y,Kobayashi N,Ishimaru T

    更新日期:2017-05-17 00:00:00

  • Photosynthetic flexibility in maize exposed to salinity and shade.

    abstract::C4 photosynthesis involves a close collaboration of the C3 and C4 metabolic cycles across the mesophyll and bundle-sheath cells. This study investigated the coordination of C4 photosynthesis in maize plants subjected to two salinity (50 and 100mM NaCl) treatments and one shade (20% of full sunlight) treatment. Photosy...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru130

    authors: Sharwood RE,Sonawane BV,Ghannoum O

    更新日期:2014-07-01 00:00:00

  • Regulation of flooding tolerance of SAG12:ipt Arabidopsis plants by cytokinin.

    abstract::A SAG12:ipt gene construct, which increases cytokinin biosynthesis in response to senescence, was introduced into Arabidopsis plants to delay senescence induced by flooding stress. Two forms of flooding stress, including total submergence and root waterlogging, were applied to SAG12:ipt (IPT) and wild-type (WT) plants...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri141

    authors: Huynh le N,Vantoai T,Streeter J,Banowetz G

    更新日期:2005-05-01 00:00:00

  • Rhizosheaths on wheat grown in acid soils: phosphorus acquisition efficiency and genetic control.

    abstract::Rhizosheaths comprise soil bound to roots, and in wheat (Triticum aestivum L.) rhizosheath size correlates with root hair length. The aims of this study were to determine the effect that a large rhizosheath has on the phosphorus (P) acquisition by wheat and to investigate the genetic control of rhizosheath size in whe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw035

    authors: James RA,Weligama C,Verbyla K,Ryan PR,Rebetzke GJ,Rattey A,Richardson AE,Delhaize E

    更新日期:2016-06-01 00:00:00

  • Cross-talk between reactive oxygen species and polyamines in regulation of ion transport across the plasma membrane: implications for plant adaptive responses.

    abstract::Many stresses are associated with increased accumulation of reactive oxygen species (ROS) and polyamines (PAs). PAs act as ROS scavengers, but export of putrescine and/or PAs to the apoplast and their catabolization by amine oxidases gives rise to H2O2 and other ROS, including hydroxyl radicals ((•)OH). PA catabolizat...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/ert423

    authors: Pottosin I,Velarde-Buendía AM,Bose J,Zepeda-Jazo I,Shabala S,Dobrovinskaya O

    更新日期:2014-03-01 00:00:00

  • Jasmonic acid deficiency leads to scattered floret opening time in cytoplasmic male sterile rice Zhenshan 97A.

    abstract::Cytoplasmic male sterile (CMS) rice has been widely used for hybrid rice seed production in China. However, CMS rice suffers from undesirable flowering habits including scattered floret opening time (FOT), which causes different FOTs among parental rice plants and greatly reduces hybrid rice seed production. Little is...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx251

    authors: Liu L,Zou Z,Qian K,Xia C,He Y,Zeng H,Zhou X,Riemann M,Yin C

    更新日期:2017-07-20 00:00:00

  • Dying to live: cell elimination as a developmental strategy in angiosperm seeds.

    abstract::The complete elimination of unwanted cells during development is a repeated theme in both multicellular animals and in plants. In plants, such events have been extensively studied and reviewed in terms of their molecular regulation, of marker genes and proteins expressed, and in terms of cellular changes associated wi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw364

    authors: Ingram GC

    更新日期:2017-02-01 00:00:00

  • Phosphate depletion modulates auxin transport in Triticum aestivum leading to altered root branching.

    abstract::Understanding the mechanisms by which nutritional signals impact upon root system architecture is a key facet in the drive for greater nutrient application efficiency in agricultural systems. Cereal plants reduce their rate of lateral root emergence under inorganic phosphate (Pi) shortage; this study uses molecular an...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru284

    authors: Talboys PJ,Healey JR,Withers PJ,Jones DL

    更新日期:2014-09-01 00:00:00

  • Alq mutation increases fruit set rate and allows the maintenance of fruit yield under moderate saline conditions.

    abstract::Arlequin (Alq) is a gain-of-function mutant whose most relevant feature is that sepals are able to become fruit-like organs due to the ectopic expression of the ALQ-TAGL1 gene. The role of this gene in tomato fruit ripening was previously demonstrated. To discover new functional roles for ALQ-TAGL1, and most particula...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz342

    authors: Ribelles C,García-Sogo B,Yuste-Lisbona FJ,Atarés A,Castañeda L,Capel C,Lozano R,Moreno V,Pineda B

    更新日期:2019-10-24 00:00:00

  • Phloem sap proteins: their identities and potential roles in the interaction between plants and phloem-feeding insects.

    abstract::The phloem is a well-known target of sucking and piercing insects that utilize the transported fluid as their major nutrient source. In addition to small molecules like sugars and amino acids, phloem sap of higher land plants contains proteins that can accumulate up to high concentrations. Although the knowledge about...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erj087

    authors: Kehr J

    更新日期:2006-01-01 00:00:00

  • Atypical iron storage in marine brown algae: a multidisciplinary study of iron transport and storage in Ectocarpus siliculosus.

    abstract::Iron is an essential element for all living organisms due to its ubiquitous role in redox and other enzymes, especially in the context of respiration and photosynthesis. The iron uptake and storage systems of terrestrial/higher plants are now reasonably well understood, with two basic strategies for iron uptake being ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers225

    authors: Böttger LH,Miller EP,Andresen C,Matzanke BF,Küpper FC,Carrano CJ

    更新日期:2012-10-01 00:00:00

  • A perspective on crassulacean acid metabolism photosynthesis evolution of orchids on different continents: Dendrobium as a case study.

    abstract::Members of the Orchidaceae, one of the largest families of flowering plants, evolved the crassulacean acid metabolism (CAM) photosynthesis strategy. It is thought that CAM triggers adaptive radiation into new niche spaces, yet very little is known about its origin and diversification on different continents. Here, we ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz461

    authors: Li MH,Liu DK,Zhang GQ,Deng H,Tu XD,Wang Y,Lan SR,Liu ZJ

    更新日期:2019-11-29 00:00:00

  • Rice H2A.Z negatively regulates genes responsive to nutrient starvation but promotes expression of key housekeeping genes.

    abstract::The H2A.Z histone variant plays a role in the modulation of environmental responses, but the nature of the associated mechanisms remains enigmatic. We investigated global H2A.Z deposition and transcriptomic changes in rice (Oryza sativa) upon exposure to phosphate (Pi) deficiency and in response to RNAi knockdown of O...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery244

    authors: Zahraeifard S,Foroozani M,Sepehri A,Oh DH,Wang G,Mangu V,Chen B,Baisakh N,Dassanayake M,Smith AP

    更新日期:2018-09-14 00:00:00

  • MAPK11 regulates seed germination and ABA signaling in tomato by phosphorylating SnRKs.

    abstract::Seed germination is a critical stage in the plant life cycle and it plays an important role in the efficiency of agricultural production. However, our knowledge of the mechanisms that regulate seed germination remains limited. In this study, we identified a novel gene, MAPK11, that encodes mitogen-activated protein ki...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa564

    authors: Song J,Shang L,Wang X,Xing Y,Xu W,Zhang Y,Wang T,Li H,Zhang J,Ye Z

    更新日期:2020-12-02 00:00:00

  • Cloning, localization and expression analysis of vacuolar sugar transporters in the CAM plant Ananas comosus (pineapple).

    abstract::In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must also be exported to...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ern077

    authors: Antony E,Taybi T,Courbot M,Mugford ST,Smith JA,Borland AM

    更新日期:2008-01-01 00:00:00

  • Development of synchronized, autonomous, and self-regulated oscillations in transpiration rate of a whole tomato plant under water stress.

    abstract::Plants respond to many environmental changes by rapidly adjusting their hydraulic conductivity and transpiration rate, thereby optimizing water-use efficiency and preventing damage due to low water potential. A multiple-load-cell apparatus, time-series analysis of the measured data, and residual low-pass filtering met...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq168

    authors: Wallach R,Da-Costa N,Raviv M,Moshelion M

    更新日期:2010-07-01 00:00:00

  • Gamma-hydroxybutyrate accumulation in Arabidopsis and tobacco plants is a general response to abiotic stress: putative regulation by redox balance and glyoxylate reductase isoforms.

    abstract::Enzymes that reduce the aldehyde chemical grouping (i.e. H-C=O) to its corresponding alcohol are probably crucial in maintaining plant health during stress. Succinic semialdehyde (SSA) is a mitochondrially-generated intermediate in the metabolism of gamma-aminobutyrate (GABA), which accumulates in response to a variet...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ern122

    authors: Allan WL,Simpson JP,Clark SM,Shelp BJ

    更新日期:2008-01-01 00:00:00

  • QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells.

    abstract::Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II int...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err211

    authors: Miao Y,Li HY,Shen J,Wang J,Jiang L

    更新日期:2011-10-01 00:00:00

  • Genetic approaches to understanding sugar-response pathways.

    abstract::Plants as photoautotrophic organisms are able to produce the carbohydrates they require and have developed mechanisms to co-ordinate carbohydrate production and its metabolism. Carbohydrate-derived signals regulate the expression of genes involved in both photosynthesis and metabolism, and control carbohydrate partiti...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erg054

    authors: Rook F,Bevan MW

    更新日期:2003-01-01 00:00:00

  • Activation of cell proliferation by brassinolide application in tobacco BY-2 cells: effects of brassinolide on cell multiplication, cell-cycle-related gene expression, and organellar DNA contents.

    abstract::Brassinosteroids (BRs) are steroidal phytohormones that are essential for many processes in plant growth and development, such as cell expansion, vascular differentiation, and responses to stress. The effects of BRs on cell division are unclear, as attested by contradictory published results. To determine the effect o...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg312

    authors: Miyazawa Y,Nakajima N,Abe T,Sakai A,Fujioka S,Kawano S,Kuroiwa T,Yoshida S

    更新日期:2003-12-01 00:00:00

  • Variation in the shoot calcium content of angiosperms.

    abstract::This study describes the variation in the mean relative shoot Ca content within the angiosperms at the ordinal level. Data were derived from studies in the literature in which the shoot Ca content of two or more species had been compared, and from a hydroponic experiment in which plants were selected to represent the ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg143

    authors: Broadley MR,Bowen HC,Cotterill HL,Hammond JP,Meacham MC,Mead A,White PJ

    更新日期:2003-05-01 00:00:00

  • Photo-oxidative stress markers as a measure of abiotic stress-induced leaf senescence: advantages and limitations.

    abstract::Inside chloroplasts, several abiotic stresses (including drought, high light, salinity, or extreme temperatures) induce a reduction in CO2 assimilation rates with a consequent increase in reactive oxygen species (ROS) production, ultimately leading to leaf senescence and yield loss. Photo-oxidation processes should th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/eru086

    authors: Pintó-Marijuan M,Munné-Bosch S

    更新日期:2014-07-01 00:00:00