Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration.

Abstract:

:Very long chain aliphatic compounds occur in the suberin polymer and associated wax. Up to now only few genes involved in suberin biosynthesis have been identified. This is a report on the isolation of a potato (Solanum tuberosum) 3-ketoacyl-CoA synthase (KCS) gene and the study of its molecular and physiological relevance by means of a reverse genetic approach. This gene, called StKCS6, was stably silenced by RNA interference (RNAi) in potato. Analysis of the chemical composition of silenced potato tuber periderms indicated that StKCS6 down-regulation has a significant and fairly specific effect on the chain length distribution of very long-chain fatty acids (VLCFAs) and derivatives, occurring in the suberin polymer and peridermal wax. All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated. Thus, StKCS6 is preferentially involved in the formation of suberin and wax lipidic monomers with chain lengths of C(28) and higher. As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type. Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

journal_name

J Exp Bot

authors

Serra O,Soler M,Hohn C,Franke R,Schreiber L,Prat S,Molinas M,Figueras M

doi

10.1093/jxb/ern314

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

697-707

issue

2

eissn

0022-0957

issn

1460-2431

pii

ern314

journal_volume

60

pub_type

杂志文章
  • Two early light-inducible protein (ELIP) cDNAs from the resurrection plant Tortula ruralis are differentially expressed in response to desiccation, rehydration, salinity, and high light.

    abstract::Tortula ruralis (Syntrichia ruralis) is a useful model system for the study of gene control in response to severe water deficit-stress. EST gene discovery efforts utilizing desiccated gametophytes have identified two cDNAs designated Elipa and Elipb with significant similarity to early light-inducible proteins (ELIPs)...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.371.1197

    authors: Zeng Q,Chen X,Wood AJ

    更新日期:2002-05-01 00:00:00

  • Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) plants grown at various salinity levels.

    abstract::Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) were studied by exposing plants to six salinity levels (0-500 mM NaCl range) for 70 d. Salt stress was administered either by pre-mixing of the calculated amount of NaCl with the potting mix before seeds were planted or by the gradual increase of NaCl l...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq257

    authors: Hariadi Y,Marandon K,Tian Y,Jacobsen SE,Shabala S

    更新日期:2011-01-01 00:00:00

  • Ethylene is required for both the initiation and progression of softening in pear (Pyrus communis L.) fruit.

    abstract::In order to investigate the physiological role of ethylene in the initiation and subsequent progression of softening, pear fruit were treated with propylene, an analogue of ethylene or 1-methylcyclopropene (1-MCP), a gaseous inhibitor of ethylene action at the preclimacteric or ripening stages. The propylene treatment...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg073

    authors: Hiwasa K,Kinugasa Y,Amano S,Hashimoto A,Nakano R,Inaba A,Kubo Y

    更新日期:2003-02-01 00:00:00

  • Dynamic changes in root hydraulic properties in response to nitrate availability.

    abstract::Changes in root hydraulic resistance in response to alterations in nitrate supply were explored in detail as a potential mechanism that allows plants to respond rapidly to changes in their environment. Sunflower (Helianthus annuus cv. Holiday) plants grown hydroponically with limited nitrate availability (200 micromol...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm118

    authors: Gloser V,Zwieniecki MA,Orians CM,Holbrook NM

    更新日期:2007-01-01 00:00:00

  • β-Carboxysome bioinformatics: identification and evolution of new bacterial microcompartment protein gene classes and core locus constraints.

    abstract::Carboxysomes are bacterial microcompartments (BMCs) that enhance CO2 fixation in all cyanobacteria. Structurally, carboxysome shell proteins are classified according to the type of oligomer formed: hexameric (BMC-H), trimeric (BMC-T) and pentameric (BMC-P) proteins. To understand the forces driving the evolution of th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx115

    authors: Sommer M,Cai F,Melnicki M,Kerfeld CA

    更新日期:2017-06-01 00:00:00

  • Ferroptosis in plants: triggers, proposed mechanisms and the role of iron in modulating cell death.

    abstract::Regulated cell death plays key roles during essential processes along the plant life cycle. It takes part of specific developmental programs and maintains the organism homeostasis in response to unfavorable environments. Ferroptosis is a recently discovered iron-dependent cell death pathway characterized by the accumu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa425

    authors: Distéfano AM,López GA,Setzes N,Marchetti F,Cainzos M,Cascallares M,Zabaleta E,Pagnussat GC

    更新日期:2020-09-11 00:00:00

  • Combining field performance with controlled environment plant imaging to identify the genetic control of growth and transpiration underlying yield response to water-deficit stress in wheat.

    abstract::Crop yield in low-rainfall environments is a complex trait under multigenic control that shows significant genotype×environment (G×E) interaction. One way to understand and track this trait is to link physiological studies to genetics by using imaging platforms to phenotype large segregating populations. A wheat popul...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv320

    authors: Parent B,Shahinnia F,Maphosa L,Berger B,Rabie H,Chalmers K,Kovalchuk A,Langridge P,Fleury D

    更新日期:2015-09-01 00:00:00

  • Overexpression of the CBF2 transcriptional activator in Arabidopsis delays leaf senescence and extends plant longevity.

    abstract::Leaf senescence is a programmed developmental process governed by various endogenous and exogenous factors, such as the plant developmental stage, leaf age, phytohormone levels, darkness, and exposure to stresses. It was found that, in addition to its well-documented role in the enhancement of plant frost tolerance, o...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp300

    authors: Sharabi-Schwager M,Lers A,Samach A,Guy CL,Porat R

    更新日期:2010-01-01 00:00:00

  • The fruit ripening-related gene FaAAT2 encodes an acyl transferase involved in strawberry aroma biogenesis.

    abstract::Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers120

    authors: Cumplido-Laso G,Medina-Puche L,Moyano E,Hoffmann T,Sinz Q,Ring L,Studart-Wittkowski C,Caballero JL,Schwab W,Muñoz-Blanco J,Blanco-Portales R

    更新日期:2012-06-01 00:00:00

  • ICE1 of Poncirus trifoliata functions in cold tolerance by modulating polyamine levels through interacting with arginine decarboxylase.

    abstract::ICE1 (Inducer of CBF Expression 1) encodes a MYC-like basic helix-loop-helix transcription factor that acts as a central regulator of cold response. In this study, we elucidated the function and underlying mechanisms of PtrICE1 from trifoliate orange [Poncirus trifoliata (L.) Raf.]. PtrICE1 was upregulated by cold, de...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv138

    authors: Huang XS,Zhang Q,Zhu D,Fu X,Wang M,Zhang Q,Moriguchi T,Liu JH

    更新日期:2015-06-01 00:00:00

  • Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues.

    abstract::Axenically grown Arabidopsis thaliana plants were analysed for the occurrence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from Arabidopsis inflorescences. In a variety of organisms, the synthesis of trehalose is catalysed by trehalose-6-...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/52.362.1817

    authors: Vogel G,Fiehn O,Jean-Richard-dit-Bressel L,Boller T,Wiemken A,Aeschbacher RA,Wingler A

    更新日期:2001-09-01 00:00:00

  • Overexpression of a putative Arabidopsis BAHD acyltransferase causes dwarfism that can be rescued by brassinosteroid.

    abstract::Plant growth and development are ensured through networks of complex regulatory schemes. Genetic approaches have been invaluable in dissecting these regulatory pathways. This study reports the isolation of a semi-dominant dwarf mutant designated abnormal shoot1-1 dominant (abs1-1D) through an Arabidopsis T-DNA activat...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers227

    authors: Wang M,Liu X,Wang R,Li W,Rodermel S,Yu F

    更新日期:2012-10-01 00:00:00

  • Light signals, phytochromes and cross-talk with other environmental cues.

    abstract::Plants have evolved highly complex sensory mechanisms to monitor their surroundings and adapt their growth and development to the prevailing environmental conditions. The integration of information from multiple environmental cues enables the co-ordination of development with favourable seasonal conditions and, ultima...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erh026

    authors: Franklin KA,Whitelam GC

    更新日期:2004-01-01 00:00:00

  • Abscisic acid regulates TSRF1-mediated resistance to Ralstonia solanacearum by modifying the expression of GCC box-containing genes in tobacco.

    abstract::Although recent studies have established a significant regulatory role for abscisic acid (ABA) and ethylene response factor (ERF) proteins in plant pathogen resistance, it is not clear whether and how ABA performs this role. Previously, it was reported that an ERF protein, TSRF1, activates the expression of GCC box-co...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm353

    authors: Zhou J,Zhang H,Yang Y,Zhang Z,Zhang H,Hu X,Chen J,Wang XC,Huang R

    更新日期:2008-01-01 00:00:00

  • Wing loading, not terminal velocity, is the best parameter to predict capacity of diaspores for secondary wind dispersal.

    abstract::Lift-off velocity may be the most useful surrogate to measure the secondary dispersal capacity of diaspores. However, the most important diaspore attribute determining diaspore lift-off velocity is unclear. Furthermore, it is not known whether terminal velocity used to characterize the primary dispersal capacity of di...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa170

    authors: Liang W,Liu Z,Liu M,Qin X,Baskin CC,Baskin JM,Xin Z,Wang Z,Su Z,Zhou Q

    更新日期:2020-07-06 00:00:00

  • Osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to Botrytis cinerea.

    abstract::Abiotic factors inducing osmotic stress can influence the plant immune response and resistance to pathogen infections. In this study, the effect of polyethylene glycol (PEG)- and sucrose-induced osmotic stress on polyamine (PA) homeostasis and the basal immune response in grapevine plantlets before and after Botrytis ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert351

    authors: Hatmi S,Trotel-Aziz P,Villaume S,Couderchet M,Clément C,Aziz A

    更新日期:2014-01-01 00:00:00

  • ALY proteins participate in multifaceted Nep1Mo-triggered responses in Nicotiana benthamiana and Arabidopsis thaliana.

    abstract::Previously, it was found that Nep1Mo (a Nep1-like protein from Magnaporthe oryzae) could trigger a variety of plant responses, including stomatal closure, hypersensitive cell death (HCD), and defence-related gene expression, in Nicotiana benthamiana. In this study, it was found that Nep1Mo-induced cell death could be ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru136

    authors: Teng W,Zhang H,Wang W,Li D,Wang M,Liu J,Zhang H,Zheng X,Zhang Z

    更新日期:2014-06-01 00:00:00

  • Evidence for long-distance xylem transport of signal peptide activity from tomato roots.

    abstract::Several types of small, endogenous signal peptides are now known to induce a wide range of local and systemic responses in plants, but how such signal peptide activity is transported over long distances remains unclear. In particular, the possible occurrence and root-to-shoot transport of signal peptide activity in th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm081

    authors: Neumann PM

    更新日期:2007-01-01 00:00:00

  • Mechanisms and function of flower and inflorescence reversion.

    abstract::Flower and inflorescence reversion involve a switch from floral development back to vegetative development, thus rendering flowering a phase in an ongoing growth pattern rather than a terminal act of the meristem. Although it can be considered an unusual event, reversion raises questions about the nature and function ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/eri254

    authors: Tooke F,Ordidge M,Chiurugwi T,Battey N

    更新日期:2005-10-01 00:00:00

  • PpEG4 is a peach endo-beta-1,4-glucanase gene whose expression in climacteric peaches does not follow a climacteric pattern.

    abstract::In peach (Prunus persica L. Batsch.) the degradation of the pectic compounds of the cell wall is considered to be the principal component responsible for fruit softening. Many genes encoding enzymes acting on the different polymers of the pectic matrix have been shown to be highly expressed during the late phases of s...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj043

    authors: Trainotti L,Pavanello A,Zanin D

    更新日期:2006-01-01 00:00:00

  • Local root ABA/cytokinin status and aquaporins regulate poplar responses to mild drought stress independently of the ectomycorrhizal fungus Laccaria bicolor.

    abstract::The relatively better performance of mycorrhizal plants subjected to drought stress has commonly been linked to improved root water uptake through the fungal regulation of plant aquaporins and hormones. In this study, we examined the role of ectomycorrhizal fungi in plant water relations and plant hormonal balance und...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz389

    authors: Calvo-Polanco M,Armada E,Zamarreño AM,García-Mina JM,Aroca R

    更新日期:2019-11-18 00:00:00

  • Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation.

    abstract::The transition from reversible to permanent wilting, in whole tomato seedlings (Lycopersicon esculentum Mill. cv. M82) following severe salt-stress by root exposure to 300 mM NaCl, was investigated. Salinized seedlings wilted rapidly but recovered if returned to non-saline nutrient solution within 6 h. However, after ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/52.364.2207

    authors: Shalata A,Neumann PM

    更新日期:2001-11-01 00:00:00

  • Phenotypic plasticity in cell walls of maize brown midrib mutants is limited by lignin composition.

    abstract::The hydrophobic cell wall polymer lignin is deposited in specialized cells to make them impermeable to water and prevent cell collapse as negative pressure or gravitational force is exerted. The variation in lignin subunit composition that exists among different species, and among different tissues within the same spe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq093

    authors: Vermerris W,Sherman DM,McIntyre LM

    更新日期:2010-05-01 00:00:00

  • Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato.

    abstract::The hormone ethylene regulates a wide range of plant developmental processes and EBF (EIN3-binding F-box) proteins were shown to negatively regulate the ethylene signalling pathway via mediating the degradation of EIN3/EIL proteins. The present study reports on the identification of two tomato F-box genes, Sl-EBF1 and...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp332

    authors: Yang Y,Wu Y,Pirrello J,Regad F,Bouzayen M,Deng W,Li Z

    更新日期:2010-03-01 00:00:00

  • Abscisic acid prevents the coalescence of protein storage vacuoles by upregulating expression of a tonoplast intrinsic protein gene in barley aleurone.

    abstract::Tonoplast intrinsic proteins (TIPs) are integral membrane proteins that are known to function in plants as aquaporins. Here, we propose another role for TIPs during the fusion of protein storage vacuoles (PSVs) in aleurone cells, a process that is promoted by gibberellic acid (GA) and prevented by abscisic acid (ABA)....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru467

    authors: Lee SE,Yim HK,Lim MN,Yoon Is,Kim Jh,Hwang YS

    更新日期:2015-03-01 00:00:00

  • Lateral root formation and growth of Arabidopsis is redundantly regulated by cytokinin metabolism and signalling genes.

    abstract::The plant root system is important for the uptake of water and nutrients and the anchoring of plants in the soil. Lateral roots (LRs) contribute considerably to root system architecture. Their post-embryonic formation is regulated by hormones and environmental cues. The hormone cytokinin influences LR formation and gr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert291

    authors: Chang L,Ramireddy E,Schmülling T

    更新日期:2013-11-01 00:00:00

  • Phloem transport: a review of mechanisms and controls.

    abstract::It is generally believed that an osmotically generated pressure gradient drives the phloem mass flow. So far, this widely accepted Münch theory has required remarkably few adaptations, but the debate on alternative and additional hypotheses is still ongoing. Recently, a possible shortcoming of the Münch theory has bee...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/ert302

    authors: De Schepper V,De Swaef T,Bauweraerts I,Steppe K

    更新日期:2013-11-01 00:00:00

  • Update on the genetic control of flowering in garden pea.

    abstract::The garden pea has been a model for the genetics of flowering for several decades and numerous flowering loci have been identified, but until recently little was known about the molecular nature of these loci. This paper presents an update on recent work on the molecular genetics of flowering in pea, outlining progres...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erp120

    authors: Weller JL,Hecht V,Liew LC,Sussmilch FC,Wenden B,Knowles CL,Vander Schoor JK

    更新日期:2009-01-01 00:00:00

  • Auxin-induced expression divergence between Arabidopsis species may originate within the TIR1/AFB-AUX/IAA-ARF module.

    abstract::Auxin is an essential regulator of plant growth and development, and auxin signaling components are conserved among land plants. Yet, a remarkable degree of natural variation in physiological and transcriptional auxin responses has been described among Arabidopsis thaliana accessions. As intraspecies comparisons offer...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw457

    authors: Trenner J,Poeschl Y,Grau J,Gogol-Döring A,Quint M,Delker C

    更新日期:2017-01-01 00:00:00

  • A chlorophyll fluorescence-based method for the integrated characterization of the photophysiological response to light stress.

    abstract::This work introduces a new experimental method for the comprehensive description of the physiological responses to light of photosynthetic organisms. It allows the integration in a single experiment of the main established manipulative chlorophyll fluorescence-based protocols. It enables the integrated characterizatio...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw492

    authors: Serôdio J,Schmidt W,Frankenbach S

    更新日期:2017-02-01 00:00:00