Abstract:
:In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a promising new tool, antagonistic peptide technology, was recently developed. Here, this antagonistic peptide technology was tested on selected CLE peptides and the related IDA peptide and its usefulness in the context of studies of peptide function discussed. Based on the analyses, it was concluded that the antagonistic peptide approach is not the ultimate means to overcome redundancy or lack of loss-of-function lines. However, information collected using antagonistic peptide approaches (in the broad sense) can be very useful, but these approaches do not work in all cases and require a deep insight on the interaction between the ligand and its receptor to be successful. This, as well as peptide ligand structure considerations, should be taken into account before ordering a wide range of synthetic peptide variants and/or generating transgenic plants.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Czyzewicz N,Wildhagen M,Cattaneo P,Stahl Y,Pinto KG,Aalen RB,Butenko MA,Simon R,Hardtke CS,De Smet Idoi
10.1093/jxb/erv284subject
Has Abstractpub_date
2015-08-01 00:00:00pages
5367-74issue
17eissn
0022-0957issn
1460-2431pii
erv284journal_volume
66pub_type
杂志文章abstract::Inflorescence stalks produced the highest amount of ethylene in response to IAA as compared with other plant parts tested. Leaf age had an effect on IAA-induced ethylene with the youngest leaves showing the greatest stimulation. The highest amount of IAA-induced ethylene was produced in the root or inflorescence tip w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern159
更新日期:2008-01-01 00:00:00
abstract::The nucleus contains the cell's genetic material, which directs cellular activity via gene regulation. The physical barrier of the nuclear envelope needs to be permeable to a variety of macromolecules and signals. The most prominent gateways for the transport of macromolecules are the nuclear pore complexes (NPCs). Th...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ers258
更新日期:2013-02-01 00:00:00
abstract::Photorespiration is an essential high flux metabolic pathway that is found in all oxygen-producing photosynthetic organisms. It is often viewed as a closed metabolic repair pathway that serves to detoxify 2-phosphoglycolic acid and to recycle carbon to fuel the Calvin-Benson cycle. However, this view is too simplistic...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw145
更新日期:2016-05-01 00:00:00
abstract::Leaves and callus of Kalanchoë daigremontiana and Taxus brevifolia were used to investigate nitric oxide-induced apoptosis in plant cells. The effect of nitric oxide (NO) was studied by using a NO donor, sodium nitroprusside (SNP), a nitric oxide-synthase (NOS) inhibitor, N:(G)-monomethyl-L-arginine (NMMA), and centri...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.347.1027
更新日期:2000-06-01 00:00:00
abstract::The reproductive success of plants largely depends on the correct programming of developmental phase transitions, particularly the shift from vegetative to reproductive growth. The timing of this transition is finely regulated by the integration of an array of environmental and endogenous factors. Nitrogen is the mine...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx423
更新日期:2018-01-23 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) cascades are involved in various processes from plant growth and development to biotic and abiotic stress responses. MAPK kinases (MAPKKs), which link MAPKs and MAPKK kinases (MAPKKKs), play crucial roles in MAPK cascades to mediate a variety of stress responses in plants. Howev...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers086
更新日期:2012-06-01 00:00:00
abstract::Ozone induces rapid activation of SIPK, a mitogen-activated protein kinase (MAPK) in tobacco. Through transgenic manipulation it has previously been shown that overexpression of SIPK leads to enhanced ozone-induced lesion formation with concomitant accumulation of ROS. In spite of this hypersensitive phenotype, the ef...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri219
更新日期:2005-08-01 00:00:00
abstract::Photoreceptors perceive different wavelengths of light and transduce light signals downstream via a range of proteins. COP1, an E3 ubiquitin ligase, regulates light signaling by mediating the ubiquitination and subsequent proteasomal degradation of photoreceptors such as phytochromes and cryptochromes, as well as vari...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx312
更新日期:2017-10-13 00:00:00
abstract::A gene MtPPRD1, encoding a protein of 132 amino acids containing a proline-rich domain (PRD), has been revealed by suppressive subtractive hybridization (SSH) with two mRNA populations of embryo axes harvested immediately before and after radicle emergence. Although at the protein level MtPPRD1 showed low homology wit...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri077
更新日期:2005-03-01 00:00:00
abstract::Chloroplasts perform many essential metabolic functions and their proper development is critically important in embryogenesis. However, little is known about how chloroplasts function in embryogenesis and more relevant components need to be characterized. In this study, we show that Arabidopsis Ribonuclease J (RNase J...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv010
更新日期:2015-04-01 00:00:00
abstract::Flowering Locus C (FLC) is a key floral repressor that precisely controls flowering time. The role of FLC has been extensively studied at the transcriptional level using molecular biological and epigenetic approaches. However, how FLC functions and how its stability is controlled at the post-translational level are on...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw431
更新日期:2017-01-01 00:00:00
abstract::Flower and inflorescence reversion involve a switch from floral development back to vegetative development, thus rendering flowering a phase in an ongoing growth pattern rather than a terminal act of the meristem. Although it can be considered an unusual event, reversion raises questions about the nature and function ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eri254
更新日期:2005-10-01 00:00:00
abstract::The aerial portion of a plant, namely the leaf, is inhabited by pathogenic and non-pathogenic microbes. The leaf's physical and chemical properties, combined with fluctuating and often challenging environmental factors, create surfaces that require a high degree of adaptation for microbial colonization. As a consequen...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa417
更新日期:2021-01-20 00:00:00
abstract::The suppression subtractive hybridization technique was used to identify differentially expressed genes between hormone-autotrophic and hormone-dependent Panax ginseng callus lines. A tonoplast intrinsic protein cDNA (PgTIP1) was found to be highly and specifically expressed in hormone-autotrophic ginseng cells, which...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl255
更新日期:2007-01-01 00:00:00
abstract::In C(3) plants, diffusion of CO(2) into leaves is restricted by stomata and subsequently by the intercellular airspaces and liquid phase into chloroplasts. While considerable information exists on the effect of environmental conditions on stomatal conductance (g(s)), little is known on whether the mesophyll conductanc...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp035
更新日期:2009-01-01 00:00:00
abstract::Plant programmed cell death (PCD) is a genetically controlled process that plays an important role in development and stress responses. Reactive oxygen species (ROS) are key inducers of PCD. The addition of 50 mM H₂O₂ to tobacco Bright Yellow-2 (TBY-2) cell cultures induces PCD. A comparative proteomic analysis of TBY...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers031
更新日期:2012-05-01 00:00:00
abstract::Hydration at low temperatures, commonly referred to as cold stratification, is widely used for releasing dormancy and triggering germination in a wide range of species including wheat. However, the molecular mechanism that underlies its effect on germination has largely remained unknown. Our previous studies showed th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw172
更新日期:2016-05-01 00:00:00
abstract::A cDNA clone encoding a 64-amino acid type 3 metallothionein protein, designated GhMT3a, was isolated from cotton (Gossypium hirsutum) by cDNA library screening. Northern blot analysis indicated that mRNA accumulation of GhMT3a was up-regulated not only by high salinity, drought, and low temperature stresses, but also...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern291
更新日期:2009-01-01 00:00:00
abstract::Although leaves and other vegetative tissues are generally considered as non-climacteric, citrus leaves show a climacteric system II behaviour after detachment. Upon harvest, young, fully expanded 'Valencia' orange (Citrus sinensis) leaves ( approximately 60-d-old) exhibited two phases of ethylene production. The firs...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri137
更新日期:2005-05-01 00:00:00
abstract::Chlorophyll (Chl) b is a ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. This pigment is synthesized from Chl a by chlorophyllide a oxygenase and plays a key role in adaptation to various environments. This study characterizes a rice mutant, pale green leaf (pgl), and isolates the gene P...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv529
更新日期:2016-03-01 00:00:00
abstract::The rootless, aquatic Utricularia species belong to the largest and most cosmopolitan carnivorous plant genus. Populations of Utricularia plants are an important component of many standing, nutrient-poor, and humic waters. Carbon (C) allocation is an aspect of Utricularia's ecophysiology that has not been studied prev...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp286
更新日期:2010-01-01 00:00:00
abstract::Particular physiological traits allow the vascular plants Deschampsia antarctica Desv. and Colobanthus quitensis (Kunth) Bartl. to inhabit Antarctica. The photosynthetic performance of these species was evaluated in situ, focusing on diffusive and biochemical constraints to CO2 assimilation. Leaf gas exchange, Chl a f...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx148
更新日期:2017-05-17 00:00:00
abstract::Somatic embryogenesis in Arabidopsis is achieved by culturing bending-cotyledon embryos on a 2,4-D-containing induction medium for 14 d followed by a transfer on to a hormone-free development medium. Several genes orthologous to Arabidopsis SHOOTMERISTEMLESS (STM), CLAVATA 1 (CLV1), and ZWILLE (ZLL) were isolated from...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq222
更新日期:2010-09-01 00:00:00
abstract::The MGOUN3(MGO3)/BRUSHY1(BRU1)/TONSOKU(TSK) gene of Arabidopsis thaliana encodes a nuclear leucine-glycine-asparagine (LGN) domain protein that may be implicated in chromatin dynamics and genome maintenance. Mutants with defects in MGO3 display a fasciated stem and disorganized meristem structures. The transition to f...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj169
更新日期:2006-01-01 00:00:00
abstract::Several root anatomical phenes affect water acquisition from drying soil, and may therefore have utility in breeding more drought-tolerant crops. Anatomical phenes that reduce the metabolic cost of the root cortex ('cortical burden') improve soil exploration and therefore water acquisition from drying soil. The best e...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru162
更新日期:2014-11-01 00:00:00
abstract::Many molecular mechanisms that regulate dormancy have been identified individually in controlled laboratory studies. However, little is known about how the seed employs this complex suite of mechanisms during dormancy cycling in the variable environment of the soil seed bank. Nevertheless, this behaviour is essential ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw477
更新日期:2017-02-01 00:00:00
abstract::Witches' broom disease (WBD) of cacao differs from other typical hemibiotrophic plant diseases by its unusually long biotrophic phase. Plant carbon sources have been proposed to regulate WBD developmental transitions; however, nothing is known about their availability at the plant-fungus interface, the apoplastic flui...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru485
更新日期:2015-03-01 00:00:00
abstract::Production per amount of water used (water use efficiency, WUE) is closely correlated with drought tolerance. Although stomatal aperture can regulate WUE, the underlying molecular mechanisms are still unclear. Previous reports revealed that stomatal closure was inhibited in the calcium-sensing receptor (CAS) antisense...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert362
更新日期:2014-01-01 00:00:00
abstract::Growing potato tubers or freshly harvested mature tubers have a dormant apical bud. Normally, this dormancy is spontaneously broken after a period of maturation of the tuber, resulting in the growth of a new sprout. Here it is shown that in in vitro-cultured growing and maturing tubers, ethanol can rapidly break this ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri245
更新日期:2005-09-01 00:00:00
abstract::RING finger proteins comprise a large family and play important roles in regulation of growth and development, hormone signalling, and responses to biotic and abiotic stresses in plants. In this study, the identification and functional characterization of a C4C4-type RING finger protein gene from the Chinese wild grap...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err253
更新日期:2011-11-01 00:00:00