Antagonistic peptide technology for functional dissection of CLE peptides revisited.

Abstract:

:In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a promising new tool, antagonistic peptide technology, was recently developed. Here, this antagonistic peptide technology was tested on selected CLE peptides and the related IDA peptide and its usefulness in the context of studies of peptide function discussed. Based on the analyses, it was concluded that the antagonistic peptide approach is not the ultimate means to overcome redundancy or lack of loss-of-function lines. However, information collected using antagonistic peptide approaches (in the broad sense) can be very useful, but these approaches do not work in all cases and require a deep insight on the interaction between the ligand and its receptor to be successful. This, as well as peptide ligand structure considerations, should be taken into account before ordering a wide range of synthetic peptide variants and/or generating transgenic plants.

journal_name

J Exp Bot

authors

Czyzewicz N,Wildhagen M,Cattaneo P,Stahl Y,Pinto KG,Aalen RB,Butenko MA,Simon R,Hardtke CS,De Smet I

doi

10.1093/jxb/erv284

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

5367-74

issue

17

eissn

0022-0957

issn

1460-2431

pii

erv284

journal_volume

66

pub_type

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