Cell sampling and analysis (SiCSA): metabolites measured at single cell resolution.

Abstract:

:By using a fine oil-filled glass microcapillary mounted on a micromanipulator, the solutes of individual plant cells can be sampled. These samples can then be analysed using a range of physical and chemical methods. Hydrostatic pressure (cell pressure probe), osmotic pressure (picolitre osmometer), organic solutes (enzyme-linked fluorescence microscope spectrometry or capillary electrophoresis), inorganic solutes (X-ray microdroplet analysis or capillary electrophoresis), (14)C (mass spectrometry), proteins (microdroplet immunoblotting), and mRNA (rt PCR) have been measured. Collectively, the battery of techniques is called single cell sampling and analysis (SiCSA) and all of the techniques have relevance to the study of plant metabolism at the resolution of the individual cell. This review summarizes the techniques for SiCSA and presents examples of applications used in this laboratory, in particular those relating to cell metabolism.

journal_name

J Exp Bot

authors

Tomos AD,Sharrock RA

keywords:

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

623-30

issue

356

eissn

0022-0957

issn

1460-2431

journal_volume

52

pub_type

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