Abstract:
:By using a fine oil-filled glass microcapillary mounted on a micromanipulator, the solutes of individual plant cells can be sampled. These samples can then be analysed using a range of physical and chemical methods. Hydrostatic pressure (cell pressure probe), osmotic pressure (picolitre osmometer), organic solutes (enzyme-linked fluorescence microscope spectrometry or capillary electrophoresis), inorganic solutes (X-ray microdroplet analysis or capillary electrophoresis), (14)C (mass spectrometry), proteins (microdroplet immunoblotting), and mRNA (rt PCR) have been measured. Collectively, the battery of techniques is called single cell sampling and analysis (SiCSA) and all of the techniques have relevance to the study of plant metabolism at the resolution of the individual cell. This review summarizes the techniques for SiCSA and presents examples of applications used in this laboratory, in particular those relating to cell metabolism.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Tomos AD,Sharrock RAkeywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
623-30issue
356eissn
0022-0957issn
1460-2431journal_volume
52pub_type
杂志文章,评审abstract::Nitrogen (N) nutrition, post-anthesis temperature and drought-induced changes in the kinetics of accumulation of dry mass, total grain N and protein fractions (albumins-globulins, amphiphils, gliadins, and glutenins) contents were examined for winter wheat (Triticum aestivum L.). Crops were grown in controlled environ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg183
更新日期:2003-07-01 00:00:00
abstract::Changes in root hydraulic resistance in response to alterations in nitrate supply were explored in detail as a potential mechanism that allows plants to respond rapidly to changes in their environment. Sunflower (Helianthus annuus cv. Holiday) plants grown hydroponically with limited nitrate availability (200 micromol...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm118
更新日期:2007-01-01 00:00:00
abstract::The effect of the complex relationship between ethylene and abscisic acid (ABA) on flower development and senescence in Hibiscus rosa-sinensis L. was investigated. Ethylene biosynthetic (HrsACS and HrsACO) and receptor (HrsETR and HrsERS) genes were isolated and their expression evaluated in three different floral tis...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err218
更新日期:2011-11-01 00:00:00
abstract::Root gravitropism is affected by many environmental stresses, including salinity, drought, and nutrient deficiency. One significant environmental stress, excess ammonium (NH(4)(+)), is well documented to inhibit root elongation and lateral root formation, yet little is known about its effects on the direction of root ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers068
更新日期:2012-06-01 00:00:00
abstract::Rice is an important crop that is very sensitive to salinity. However, some varieties differ greatly in this feature, making investigations of salinity tolerance mechanisms possible. The cultivar Pokkali is salinity tolerant and is known to have more extensive hydrophobic barriers in its roots than does IR20, a more s...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err135
更新日期:2011-08-01 00:00:00
abstract::The disruption of K(+) transport and accumulation is symptomatic of NH(4)(+) toxicity in plants. In this study, the influence of K(+) supply (0.02-40 mM) and nitrogen source (10 mM NH(4)(+) or NO(3)(-)) on root plasma membrane K(+) fluxes and cytosolic K(+) pools, plant growth, and whole-plant K(+) distribution in the...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern190
更新日期:2008-01-01 00:00:00
abstract::Leaves and callus of Kalanchoë daigremontiana and Taxus brevifolia were used to investigate nitric oxide-induced apoptosis in plant cells. The effect of nitric oxide (NO) was studied by using a NO donor, sodium nitroprusside (SNP), a nitric oxide-synthase (NOS) inhibitor, N:(G)-monomethyl-L-arginine (NMMA), and centri...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.347.1027
更新日期:2000-06-01 00:00:00
abstract::Soil drying and re-wetting (DRW) occurs at varying frequencies and intensities during crop production, and is deliberately used in water-saving irrigation techniques that aim to enhance crop water use efficiency. Soil drying not only limits root water uptake which can (but not always) perturb shoot water status, but a...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru532
更新日期:2015-04-01 00:00:00
abstract::ω-3 fatty acid desaturases (FADs) are thought to contribute to plant stress tolerance mainly through linolenic acid (C18:3)-induced membrane stabilization, but a comprehensive analysis of their roles in stress adaptation is lacking. Here, we isolated a microsomal ω-3 FAD gene (CbFAD3) from a cryophyte (Chorispora bung...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery050
更新日期:2018-04-09 00:00:00
abstract::The expression and activity of glutamine synthetase (GS, EC 6.3.1.2) were examined in relation to the rate of CO2 assimilation in sunflower (Helianthus annuus L.) leaves. Intact plants were kept in the dark for 72 h and subsequently exposed to light under different atmospheric CO2 concentrations (100, 400 and 1200 mic...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh017
更新日期:2004-01-01 00:00:00
abstract::RNA polymerase II (RNAPII) is responsible for the transcription of most eukaryotic protein-coding genes. Analysing the topological distribution and quantification of RNAPII can contribute to understanding its function in interphase nuclei. Previously it was shown that RNAPII molecules in plant nuclei form reticulate s...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv091
更新日期:2015-03-01 00:00:00
abstract::Bud break in raspberry (Rubus idaeus L.) is often poor and uneven, with many of the subapical buds remaining in a dormant state. In order to determine the dormancy status of raspberry buds, an empirical measure of bud burst in a growth-permissive environment following exposure to chilling (4 degrees C cold storage) wa...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl266
更新日期:2007-01-01 00:00:00
abstract::Adventitious roots (ARs) are important for some plants that depend on clonal propagation. In this study, we demonstrate that a salt-responsive gene module is involved in the negative regulation of AR development in poplar. In this module, the expression of bZIP53 is induced by salt stress and it encodes a transcriptio...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa096
更新日期:2020-06-22 00:00:00
abstract::Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress. Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance. This study aimed to determine whether galactin...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz450
更新日期:2020-01-01 00:00:00
abstract::Cortical microtubules (CMTs) are often aligned in a particular direction in individual cells or even in groups of cells and play a central role in the definition of growth anisotropy. How the CMTs themselves are aligned is not well known, but two hypotheses have been proposed. According to the first hypothesis, CMTs a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert352
更新日期:2013-12-01 00:00:00
abstract::Our current understanding of how plants move auxin through their tissues is largely built on the use of polar auxin transporter inhibitors. Although the most important proteins that mediate auxin transport and its regulation have probably all been identified and the mapping of their interactions is well underway, mech...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erx323
更新日期:2018-01-04 00:00:00
abstract::The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxid...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp256
更新日期:2009-01-01 00:00:00
abstract::Over the last two decades, extensive studies have been performed at the molecular level to understand the evolution of carnivorous plants. As fruits, the repertoires of protein components in the digestive fluids of several carnivorous plants have gradually become clear. However, the quantitative aspect of these protei...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa560
更新日期:2020-11-28 00:00:00
abstract::CO(2) faces a series of resistances while diffusing between the substomatal cavities and the sites of carboxylation within chloroplasts. The absence of techniques to measure the resistance of individual steps makes it difficult to define their relative importance. Resistance to diffusion through intercellular airspace...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erp117
更新日期:2009-01-01 00:00:00
abstract::Temporal compartmentation of carboxylation processes is a defining feature of crassulacean acid metabolism and involves circadian control of key metabolic and transport steps that regulate the supply and demand for carbon over a 24h cycle. Recent insights on the molecular workings of the circadian clock and its connec...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru185
更新日期:2014-07-01 00:00:00
abstract::While the general effect of CO2 enrichment on photosynthesis, stomatal conductance, N content, and yield has been documented, there is still some uncertainty as to whether there are interactive effects between CO2 enrichment and other factors, such as temperature, geographical location, water availability, and cultiva...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa330
更新日期:2020-10-07 00:00:00
abstract::Several kinds of signal may be generated when roots are exposed to an environmental stress. Some, but not all, are conveyed to shoots in the transpiration stream. Principles are summarized that may help establish experimentally the presence or intensity of root signals transported by transpiration. In many dryland spe...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jexbot/53.367.175
更新日期:2002-02-01 00:00:00
abstract::Virus-induced gene silencing (VIGS) is a useful tool for functional characterization of genes in plants. Unfortunately, the efficiency of infection by Tobacco rattle virus (TRV) is relatively low for some non-Solanaceae plants, which are economically important, such as rose (Rosa sp.). Here, to generate an easy tracea...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert381
更新日期:2014-01-01 00:00:00
abstract::Rapid alkalinization factor (RALF) is a peptide signal that plays a basic role in cell biology and most likely regulates cell expansion. In this study, transgenic Arabidopsis thaliana lines with high and low levels of AtRALF1 transcripts were used to investigate this peptide's mechanism of action. Overexpression of th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru099
更新日期:2014-05-01 00:00:00
abstract::The response of stomata to many environmental factors is well documented. Multiple signalling pathways for abscisic acid (ABA)-induced stomatal closure have been proposed over the last decades. However, it seems that exposure of a leaf for a long time (several days) to some environmental conditions generates a sort of...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert192
更新日期:2013-09-01 00:00:00
abstract::Brassica rapa includes several important leafy vegetable crops with the potential for high cadmium (Cd) accumulation, posing a risk to human health. This study aims to understand the genetic basis underlying the variation in Cd accumulation among B. rapa vegetables. Cd uptake and translocation in 64 B. rapa accessions...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz310
更新日期:2019-10-24 00:00:00
abstract::Tobacco plants were transformed by leaf disc regeneration with the VvHT1 (Vitis vinifera hexose transporter 1) cDNA under the control of the constitutive CaMV 35S promoter in a sense or antisense orientation. Among the 20 sense plants and 10 antisense plants obtained, two sense plants showed a mutant phenotype when gr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg119
更新日期:2003-04-01 00:00:00
abstract::PaHB1 (for Picea abies Homeobox1), an evolutionarily conserved HD-GL2 homeobox gene, specifically expressed in the protoderm during somatic embryogenesis in the gymnosperm Norway spruce has been reported previously. An additional HD-GL2 gene designated PaHB2 is reported here. During somatic embryogenesis, the PaHB2 ge...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg145
更新日期:2003-05-01 00:00:00
abstract::Sunflower (Helianthus annuus L. cv. SH222) plants and calli were exposed to KCl stress for three weeks. Calli were more tolerant to KCl than plants. KCl stress decreased NO(-)(3), Mn, Fe and B levels in whole plants and P, Ca and Mg in shoots. NO(-)(3), P, Ca, Mg, Mn, and B levels decreased in 100 mM-stressed calli. C...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthes...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw045
更新日期:2016-04-01 00:00:00