Abstract:
:Tobacco plants were transformed by leaf disc regeneration with the VvHT1 (Vitis vinifera hexose transporter 1) cDNA under the control of the constitutive CaMV 35S promoter in a sense or antisense orientation. Among the 20 sense plants and 10 antisense plants obtained, two sense plants showed a mutant phenotype when grown in vitro, with stunted growth and an increase in the (leaves+stem)/roots dry weight ratio. The rate of [(3)H]-glucose uptake in leaf discs from these plants was decreased to 25% of the value measured in control plants. The amount of VvHT1 transgene and of host monosaccharide transporter MST transcripts in the leaves were studied by RNA gel blot analysis. The VvHT1 transcripts were usually present, but the amount of MST transcripts was the lowest in the plants that exhibited the most marked phenotype. Although the phenotype was lost when the plants were transferred from in vitro to greenhouse conditions, it was found again in vitro in the progeny obtained by self-pollination or by back-cross. The data show that VvHT1 sense expression resulted in unidirectional post-transcriptional gene inactivation of MST in some of the transformants, with dramatic effects on growth. They provide the first example of plants modified for hexose transport by post-transcriptional gene silencing. Some of the antisense plants also showed reduced expression of MST, and decreased growth. These results indicate that, like the sucrose transporters, hexose transporters play an important role in assimilate transport and in morphogenesis.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Leterrier M,Atanassova R,Laquitaine L,Gaillard C,Coutos-Thévenot P,Delrot Sdoi
10.1093/jxb/erg119keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
1193-204issue
385eissn
0022-0957issn
1460-2431journal_volume
54pub_type
杂志文章abstract::Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers that regulate developmental pathways in plants. PcGs form nuclear multi-subunit Polycomb Repressive Complexes (PRCs). The PRC2 complex mediates gene repression via methylation of lysine 27 on histone H3, which consequently leads to chromati...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw373
更新日期:2016-11-01 00:00:00
abstract::In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth. Using in situ immunolocalization and size exclusion epitope detection chrom...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz370
更新日期:2019-11-18 00:00:00
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journal_title:Journal of experimental botany
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更新日期:2004-08-01 00:00:00
abstract::Photosynthesis has become a major trait of interest for cereal yield improvement as breeders appear to have reached the theoretical genetic limit for harvest index, the mass of grain as a proportion of crop biomass. Yield improvements afforded by the adoption of green revolution dwarfing genes to wheat and rice are be...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa077
更新日期:2020-04-06 00:00:00
abstract::Magnesium-chelatase H subunit [CHLH/putative abscisic acid (ABA) receptor ABAR] positively regulates guard cell signalling in response to ABA, but the molecular mechanism remains largely unknown. A member of the sucrose nonfermenting 1 (SNF1)-related protein kinase 2 family, SnRK2.6/open stomata 1 (OST1)/SRK2E, which ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv341
更新日期:2015-10-01 00:00:00
abstract::Sexual reproduction in higher plants uses pollination, involving interactions between pollen and pistil. Self-incompatibility (SI) prevents self-fertilization, providing an important mechanism to promote outbreeding. SI is controlled by the S-locus; discrimination occurs between incompatible pollen, which is rejected,...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru225
更新日期:2014-08-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw316
更新日期:2016-10-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers265
更新日期:2012-10-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
abstract::Chlorophyll (Chl) b is a ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. This pigment is synthesized from Chl a by chlorophyllide a oxygenase and plays a key role in adaptation to various environments. This study characterizes a rice mutant, pale green leaf (pgl), and isolates the gene P...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv529
更新日期:2016-03-01 00:00:00
abstract::Water is a principal limitation to agricultural production during drought and in arid regions of the world. Mechanisms that plants use to cope with drought can be grouped into two different strategies: drought tolerance and drought avoidance. Previous efforts toward engineering plants for improved performance during d...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.369.699
更新日期:2002-04-01 00:00:00
abstract::The aim of this work was to investigate the importance of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) in potato carbohydrate metabolism. For this purpose, the cytosolic isoform of phosphorylating GAPC was cloned and used for an antisense approach to generate transgenic potato plants that ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj207
更新日期:2006-01-01 00:00:00
abstract::Previously, the OEP16.1 channel pore in the outer envelope membrane of mature pea (Pisum sativum) chloroplasts in vitro has been characterized to be selective for amino acids. Isolation of OEP16.2, a second OEP16 isoform from pea, in the current study allowed membrane localization and gene expression of OEP16 to be fo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err375
更新日期:2012-03-01 00:00:00
abstract::Cell wall apposition (CWA) formation is one of the first lines of defence used by plants to halt invading fungi such as powdery mildew. Lignin is a complex polymer of hydroxylated and methoxylated phenylpropane units (monolignols) and lignification renders the cell wall more resistant to pathogen attack. The role of m...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern290
更新日期:2009-01-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm371
更新日期:2008-01-01 00:00:00
abstract::Voltage dependence of ionic currents and ion fluxes in a walled, turgor-regulating cell were measured in Neurospora crassa. The hyphal morphology of the model organism Neurospora simplifies cable analysis of ionic currents to determine current density for quantitative comparisons with ion fluxes. The ion fluxes were m...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm204
更新日期:2007-01-01 00:00:00
abstract::Many biotypes of the northern-hemisphere Arctic-Alpine grass Poa alpina L. reproduce asexually via prolification of the spikelet axis to produce dehiscing shoots. Although capable of photosynthesis, the source-sink characteristics of these synflorescence systems are unknown, including the degree to which plantlets fro...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.351.1705
更新日期:2000-10-01 00:00:00
abstract::In contrast to desiccation-tolerant 'orthodox' seeds, so-called 'intermediate' seeds cannot survive complete drying and are short-lived. All species of the genus Coffea produce intermediate seeds, but they show a considerable variability in seed desiccation tolerance (DT), which may help to decipher the molecular basi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz508
更新日期:2020-02-19 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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更新日期:2018-01-23 00:00:00
abstract::In taproot of oilseed rape (Brassica napus L.), a 23 kDa polypeptide has been recently identified as a putative vegetative storage protein (VSP) because of its accumulation during flowering and its specific mobilization to sustain grain filling when N uptake is strongly reduced. The objectives were to characterize thi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.367.265
更新日期:2002-02-01 00:00:00
abstract::The aleurone layer of cereal grains is important biologically as well as nutritionally and economically. Here, current knowledge on the regulation of aleurone development is reviewed. Recent reports suggest that the control of aleurone development is more complex than earlier models portrayed. Multiple levels of genet...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erq372
更新日期:2011-03-01 00:00:00
abstract::Nitrate and ammonia deferentially modulate primary metabolism during the hypersensitive response in tobacco. In this study, tobacco RNAi lines with low nitrite reductase (NiRr) levels were used to investigate the roles of nitrite and nitric oxide (NO) in this process. The lines accumulate NO2-, with increased NO gener...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz161
更新日期:2019-08-29 00:00:00
abstract::The genetic basis of stem cell specification in somatic embryogenesis and organogenesis is still obscure. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes are involved in embryogenesis and organogenesis in numerous species. In vitro culture of Cyclamen persicum immature ovules provides a system for investigatin...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err295
更新日期:2012-01-01 00:00:00
abstract::A powerful technique is described to localize the activities of a range of enzymes in a wide variety of plant tissues. The method is based on the coupling of the enzymatic reaction to the reduction of NAD and subsequent reduction and precipitation of nitroblue tetrazolium. Enzymes that did not reduce NAD could be visu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.367.361
更新日期:2002-02-01 00:00:00
abstract::In Arabidopsis thaliana, Argonaute1 (AGO1) interacts with miR168 to modulate the small RNA regulatory pathway. However, the underlying mechanism of regulation and relationship between AGO1 and miR168 is poorly understood in the cash crop Solanum lycopersicum (tomato). We previously found that SlAGO1A and SlAGO1B were ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru387
更新日期:2014-12-01 00:00:00
abstract::Cereal grain germination is central for plant early development, and efficient germination has a major role in crop propagation and malting. Endosperm starch is the prime energy reserve in germination and seedling establishment. In this study, it was hypothesized that optimized starch granule structure, and not only t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru107
更新日期:2014-06-01 00:00:00
abstract::Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq040
更新日期:2010-06-01 00:00:00
abstract::The physiological activity of exogenous 4-Cl-IAA, as compared to IAA, was examined in maize coleoptile segments. It was found that in this model system 4-Cl-IAA is much more active in the stimulation of elongation than IAA. Simultaneous measurements of growth and external pH indicated that administration of either IAA...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.371.1089
更新日期:2002-05-01 00:00:00
abstract::The present work describes the intrachloroplast localization and the changes that took place in the thylakoid and stroma-located superoxide dismutases (SOD, EC 1.15.1.1) and ascorbate peroxidases (APX, EC 1.11.1.11), in response to long-term NaCl stress in Pisum sativum L. cv. Puget plants. Native PAGE using high chlo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh013
更新日期:2004-01-01 00:00:00