Abstract:
:The present work describes the intrachloroplast localization and the changes that took place in the thylakoid and stroma-located superoxide dismutases (SOD, EC 1.15.1.1) and ascorbate peroxidases (APX, EC 1.11.1.11), in response to long-term NaCl stress in Pisum sativum L. cv. Puget plants. Native PAGE using high chloroplast protein concentrations pointed to the presence of the two main Fe-SODs, together with CuZn-SODs, both in thylakoids and in the stroma. Western blot and immunogold labelling using the antibodies against chloroplastic Fe-SOD from Nuphar luteum also confirmed the chloroplastic localization of a Fe-SOD. Thylakoidal Fe-SOD activity was induced by a NaCl concentration as low as 70 mM, while CuZn-SOD was induced at 90 mM, although in severe stress conditions (110 mM) both activities were similar to the levels at 90 mM NaCl. NaCl stress also induced stromatic Fe-SOD and CuZn-SOD activities, although these inductions only started at higher NaCl concentration (90 mM) and were significant at 110 mM NaCl. The increase in activity of both Fe-SODs was matched by an increase in Fe-SOD protein. Chloroplastic APX isoenzymes behaved differently in thylakoids and stroma in response to NaCl. A significant increase of stromal APX occurred at 70 mM, whereas the thylakoidal APX activity was significantly and progressively lost in response to NaCl stress (70-110 mM). A significant increase in the H2O2 content of chloroplasts during stress and a reduction in the ascorbate level at 90 mM NaCl also took place, although the oxidized ascorbate pool at the highest NaCl concentration did not show significant changes. These results suggest that the loss of thylakoidal APX may be an important factor in the increase in chloroplastic H2O2, which also results from the increased thylakoid and stroma-located Fe-SOD and CuZn-SOD activities. This H2O2 may be involved in the induction of stromal APX. The up-regulation of the above enzymes in the described stress conditions would contribute to the adaptation of cv. Puget plants to moderate NaCl stress.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Gomez JM,Jimenez A,Olmos E,Sevilla Fdoi
10.1093/jxb/erh013keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
119-30issue
394eissn
0022-0957issn
1460-2431pii
55/394/119journal_volume
55pub_type
杂志文章abstract::Small signalling peptides have emerged as important cell to cell messengers in plant development and stress responses. However, only a few of the predicted peptides have been functionally characterized. Here, we present functional characterization of two members of the IDA-LIKE (IDL) peptide family in Arabidopsis thal...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx168
更新日期:2017-06-15 00:00:00
abstract::The root cap assists the passage of the root through soil by means of its slimy mucilage secretion and by the sloughing of its outer cells. The root penetration resistance of decapped primary roots of maize (Zea mays L. cv. Mephisto) was compared with that of intact roots in loose (dry bulk density 1.0 g cm-3; penetra...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg226
更新日期:2003-09-01 00:00:00
abstract::Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers120
更新日期:2012-06-01 00:00:00
abstract::Miscanthus × giganteus grown in cool temperate regions of North America and Europe can exhibit severe mortality in the year after planting, and poor frost tolerance of leaves. Spartina pectinata (prairie cordgrass), a productive C4 perennial grass native to North America, has been suggested as an alternative biofuel f...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv085
更新日期:2015-07-01 00:00:00
abstract::Iron (Fe) is vital for plant growth. Plants balance the beneficial and toxic effects of this micronutrient, and tightly control Fe uptake and allocation. Here, we review the role of the basic helix-loop-helix (bHLH) transcription factor FIT (FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) in Fe acquisition. FIT i...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa012
更新日期:2020-03-12 00:00:00
abstract::Grape berry development and ripening are under complex regulation by the nutrients, hormones, and environment cues sensed by the berry. However, the biochemical and molecular mechanisms underlying these types of regulation are poorly understood. A simplified but realistic model system that enables fruit growth conditi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert489
更新日期:2014-08-01 00:00:00
abstract::(1,3;1,4)-β-D-glucans (mixed-linkage glucans) are found in tissues of members of the Poaceae (grasses), and are particularly high in barley (Hordeum vulgare) grains. The present study describes the isolation of three independent (1,3;1,4)-β-D-glucanless (betaglucanless; bgl) mutants of barley which completely lack (1,...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err285
更新日期:2012-01-01 00:00:00
abstract::Pectins are major components of plant primary cell walls. They include homogalacturonans (HGs), which are the most abundant pectin and can be the target of apoplastic enzymes like pectin methylesterases (PMEs) that control their methylesterification level. Several PMEs are expressed in the seed coat of Arabidopsis tha...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw025
更新日期:2016-04-01 00:00:00
abstract::(13)C discrimination in organic matter with respect to atmospheric CO(2) (Delta(13)C) is under tight genetic control in many plant species, including the pedunculate oak (Quercus robur L.) full-sib progeny used in this study. Delta(13)C is expected to reflect intrinsic water use efficiency, but this assumption require...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp100
更新日期:2009-01-01 00:00:00
abstract::Plants have fundamental dependences on nitrogen and sulfur and frequently have to cope with chronic limitations when their supply is sub-optimal. This study aimed at characterizing the metabolomic, proteomic, and transcriptomic changes occurring in Arabidopsis leaves under chronic nitrate (Low-N) and chronic sulfate (...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa337
更新日期:2020-10-22 00:00:00
abstract::Seed germination is often induced by a pulse of red light perceived by phytochrome and cancelled by a subsequent pulse of far-red light. When the pulse of red light is followed by several hours of darkness, a pulse of far-red light is no longer effective and prolonged far-red is necessary to block germination. The aim...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.347.1127
更新日期:2000-06-01 00:00:00
abstract::The plant family Cactaceae is considered among the most threatened groups of organisms on the planet. The threatened status of the cacti family has created a renewed interest in the highly evolved physiological and morphological traits that underpin their persistence in some of the harshest subtropical environments in...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erz320
更新日期:2019-11-29 00:00:00
abstract::Ozone induces rapid activation of SIPK, a mitogen-activated protein kinase (MAPK) in tobacco. Through transgenic manipulation it has previously been shown that overexpression of SIPK leads to enhanced ozone-induced lesion formation with concomitant accumulation of ROS. In spite of this hypersensitive phenotype, the ef...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri219
更新日期:2005-08-01 00:00:00
abstract::Particular physiological traits allow the vascular plants Deschampsia antarctica Desv. and Colobanthus quitensis (Kunth) Bartl. to inhabit Antarctica. The photosynthetic performance of these species was evaluated in situ, focusing on diffusive and biochemical constraints to CO2 assimilation. Leaf gas exchange, Chl a f...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx148
更新日期:2017-05-17 00:00:00
abstract::Plasma membrane (PM) H+-ATPase is essential for plant growth and development. Various environmental stimuli regulate its activity, a process that involves many protein cofactors. However, whether endogenous small molecules play a role in this regulation remains unknown. Here, we describe a bio-guided isolation method ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx156
更新日期:2017-05-17 00:00:00
abstract::This paper illustrates the possibility of measuring chlorophyll (Chl) content and Chl fluorescence parameters by the solar-induced Chl fluorescence (SIF) method using the Fraunhofer line depth (FLD) principle, and compares the results with the standard measurement methods. A high-spectral resolution HR2000+ and an ord...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv272
更新日期:2015-09-01 00:00:00
abstract::Plant-parasitic root-knot nematodes induce the formation of giant cells within the plant root, and it has been recognized that auxin accumulates in these feeding sites. Here, we studied the role of the auxin transport system governed by AUX1/LAX3 influx proteins and different PIN efflux proteins during feeding site de...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw230
更新日期:2016-08-01 00:00:00
abstract::In grapevine, stimulation of defence responses was evidenced in response to various types of abiotic stresses in both leaves and berries, as revealed by the increasing expression of genes encoding defence-related proteins or the stimulation of their corresponding activities. However, the capability of inflorescences t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern361
更新日期:2009-01-01 00:00:00
abstract::Zinc (Zn), an essential nutrient in cells, plays a vital role in controlling cellular processes such as growth, development, and differentiation. Although the mechanisms of Zn translocation in rice plants (Oryza sativa) are not fully understood, it has recently received increased interest. OsZIP4 is a Zn transporter t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm147
更新日期:2007-01-01 00:00:00
abstract::C4 photosynthesis involves a close collaboration of the C3 and C4 metabolic cycles across the mesophyll and bundle-sheath cells. This study investigated the coordination of C4 photosynthesis in maize plants subjected to two salinity (50 and 100mM NaCl) treatments and one shade (20% of full sunlight) treatment. Photosy...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru130
更新日期:2014-07-01 00:00:00
abstract::Leaf angle (LA) is a critical agronomic trait in maize, with more upright leaves allowing higher planting density, leading to more efficient light capture and higher yields. A few genes responsible for variation in LA have been identified by map-based cloning. In this study, we cloned maize ZmIBH1-1, which encodes a b...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa052
更新日期:2020-05-30 00:00:00
abstract::The carbohydrate moieties of arabinogalactan proteins (AGPs) are essential for their physiological functions and undergo rapid turnover in vivo. Degradation of the carbohydrate moieties of AGPs seems to occur by concerted action of several glycosidases, among them alpha-L-arabinofuranosidase, beta-D-galactosidase, and...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj206
更新日期:2006-01-01 00:00:00
abstract::The protein encoded by the Arabidopsis At3g55360 gene was selected as a candidate for the enoyl reductase of the microsomal elongase system based on its homology to the Tsc13p protein of S. cerevisiae. The studies presented here demonstrate that heterologous expression of At3g55360 functionally complements the tempera...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh061
更新日期:2004-02-01 00:00:00
abstract::Plant root rhizosphere interactions with mutualistic microbes are diverse and numerous, having evolved over time in response to selective pressures on plants to attain anchorage and nutrients. These relationships can be considered to be formed through a combination of architectural connections: molecular architecture ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv038
更新日期:2015-04-01 00:00:00
abstract::Photorespiration is an essential high flux metabolic pathway that is found in all oxygen-producing photosynthetic organisms. It is often viewed as a closed metabolic repair pathway that serves to detoxify 2-phosphoglycolic acid and to recycle carbon to fuel the Calvin-Benson cycle. However, this view is too simplistic...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw145
更新日期:2016-05-01 00:00:00
abstract::Many stresses are associated with increased accumulation of reactive oxygen species (ROS) and polyamines (PAs). PAs act as ROS scavengers, but export of putrescine and/or PAs to the apoplast and their catabolization by amine oxidases gives rise to H2O2 and other ROS, including hydroxyl radicals ((•)OH). PA catabolizat...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ert423
更新日期:2014-03-01 00:00:00
abstract::The harpin protein Hpa1 has multiple beneficial effects in plants, promoting plant growth and development, increasing crop yield, and inducing resistance to pathogens and insect pests. For these effects, the 10-40 residue fragment (Hpa1₁₀₋₄₂) isolated from the Hpa1 sequence is 1.3- to 7.5-fold more effective than the ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert488
更新日期:2014-04-01 00:00:00
abstract::Plants have developed diverse defence mechanisms to ward off herbivorous pests. However, agriculture still faces estimated crop yield losses ranging from 25% to 40% annually. These losses arise not only because of direct feeding damage, but also because many pests serve as vectors of plant viruses. Herbivorous thrips ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ery060
更新日期:2018-04-09 00:00:00
abstract::The efficient exclusion of excess Na from the cytoplasm and vacuolar Na(+) accumulation are the main mechanisms for the adaptation of plants to salt stress. This is typically carried out by transmembrane transport proteins that exclude Na(+) from the cytosol in exchange for H(+), a secondary transport process which is...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp011
更新日期:2009-01-01 00:00:00
abstract::Plant U-box type E3 ubiquitin ligases (PUBs) are well known for their functions in a variety of stress responses, including immune responses and the adaptation to abiotic stresses. First linked to pollen self-incompatibility, their repertoire of roles has grown to encompass also the regulation of developmental process...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erx411
更新日期:2018-01-23 00:00:00