Abstract:
:The efficient exclusion of excess Na from the cytoplasm and vacuolar Na(+) accumulation are the main mechanisms for the adaptation of plants to salt stress. This is typically carried out by transmembrane transport proteins that exclude Na(+) from the cytosol in exchange for H(+), a secondary transport process which is energy-dependent and driven by the proton-motive force generated by plasma-membrane and tonoplast proton pumps. Tonoplast enriched-vesicles from control and 150 mM NaCl-tolerant calli lines were used as a model system to study the activity of V-H(+)-PPase and V-H(+)-ATPase and the involvement of Na(+) compartmentalization into the vacuole as a mechanism of salt tolerance in Solanum tuberosum. Both ATP- and pyrophosphate (PP(i))-dependent H(+)-transport were higher in tonoplast vesicles from the salt-tolerant line than in vesicles from control cells. Western blotting of tonoplast proteins confirmed that changes in V-H(+)-PPase activity are correlated with increased protein amount. Conversely, immunodetection of the A-subunit of V-H(+)-ATPase revealed that a mechanism of post-translational regulation is probably involved. Na(+)-dependent dissipation of a pre-established pH gradient was used to measure Na(+)/H(+) exchange in tonoplast vesicles. The initial rates of proton efflux followed Michaelis-Menten kinetics and the V(max) of proton dissipation was 2-fold higher in NaCl-tolerant calli when compared to the control. H(+)-coupled exchange was specific for Na(+) and Li(+) and not for K(+). The increase of both the pH gradient across the tonoplast and the Na(+)/H(+) antiport activity in response to salt strongly suggests that Na(+) sequestration into the vacuole contributes to salt tolerance in potato.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Queirós F,Fontes N,Silva P,Almeida D,Maeshima M,Gerós H,Fidalgo Fdoi
10.1093/jxb/erp011subject
Has Abstractpub_date
2009-01-01 00:00:00pages
1363-74issue
4eissn
0022-0957issn
1460-2431pii
erp011journal_volume
60pub_type
杂志文章abstract::SPICK2, a homologue of the weakly-inward-rectifying Shaker-like Arabidopsis K channel, AKT2, is a candidate K+-influx channel participating in light- and clock-regulated leaf movements of the legume, Samanea saman. Light and the biological clock regulate the in situ K+-influx channel activity differentially in extenso...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl104
更新日期:2006-01-01 00:00:00
abstract::The ability of two soybean (Glycine max L. [Merrill]) cultivars, 'Williams 82' and 'Maple Arrow', which were reported to use different ureide degradation pathways, to degrade the ureides allantoin and allantoate was investigated. Protein fractions and total leaf homogenates from the fourth trifoliate leaves of both cu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh100
更新日期:2004-04-01 00:00:00
abstract::Dry matter partitioning is the end result of the flow of assimilates from source organs via a transport path to the sink organs. The dry matter partitioning among the sinks of a plant is primarily regulated by the sinks themselves. The effect of source strength on dry matter partitioning is often not a direct one, but...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/47.Special_Issue.1281
更新日期:1996-08-01 00:00:00
abstract::The possible role of photorespiration as a general stress protection mechanism, and in C(4) plant metabolism, is controversial. In particular, the potential involvement of photorespiration in the acquisition of desiccation tolerance in 'resurrection' plants is unknown. An investigation was carried out into whether pho...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm247
更新日期:2007-01-01 00:00:00
abstract::Abscission is triggered by multiple environmental and developmental cues, including endogenous plant hormones. KNOTTED-LIKE HOMEOBOX (KNOX) transcription factors (TFs) play an important role in controlling abscission in plants. However, the underlying molecular mechanism of KNOX TFs in abscission is largely unknown. H...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa162
更新日期:2020-07-06 00:00:00
abstract::In peach trees (Prunus persica L. Batsch cv. Redhaven), sorbitol is a primary photosynthetic product and may play an important role in the budbreak process. Surprisingly, before budbreak (from January to early March), the concentration of sorbitol in the xylem sap decreases, while that of hexoses (glucose and fructose...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh087
更新日期:2004-04-01 00:00:00
abstract::Shipping of peaches to distant markets and storage require low temperature; however, cold storage affects fruit quality causing physiological disorders collectively termed 'chilling injury' (CI). In order to ameliorate CI, different strategies have been applied before cold storage; among them heat treatment (HT) has b...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp267
更新日期:2009-01-01 00:00:00
abstract::Proline metabolism is important for environmental responses, plant growth, and development. However, its precise roles in plant abiotic stress tolerance are not well understood. Mutants are valuable for the identification of new genes and for elucidating their roles in physiological mechanisms. We applied a suppressor...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx069
更新日期:2017-03-01 00:00:00
abstract::Root phenotyping provides trait information for plant breeding. A shortcoming of high-throughput root phenotyping is the limitation to seedling plants and failure to make inferences on mature root systems. We suggest root system architecture (RSA) models to predict mature root traits and overcome the inference problem...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw494
更新日期:2017-02-01 00:00:00
abstract::Ethylene production and expression of ethylene biosynthetic genes was investigated in senescing flowers of carnation (Dianthus caryophyllus L.) cultivars 'White Candle (WC)' and 'Light Pink Barbara (LPB)', with long and short vase-lives, respectively. Ethylene production from the gynoecium and petals of senescing 'WC'...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh081
更新日期:2004-03-01 00:00:00
abstract::Abiotic factors inducing osmotic stress can influence the plant immune response and resistance to pathogen infections. In this study, the effect of polyethylene glycol (PEG)- and sucrose-induced osmotic stress on polyamine (PA) homeostasis and the basal immune response in grapevine plantlets before and after Botrytis ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert351
更新日期:2014-01-01 00:00:00
abstract::Plants show varied cellular responses to salinity that are partly associated with maintaining low cytosolic Na(+) levels and a high K(+)/Na(+) ratio. Plant metabolites change with elevated Na(+), some changes are likely to help restore osmotic balance while others protect Na(+)-sensitive proteins. Metabolic responses ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp243
更新日期:2009-01-01 00:00:00
abstract::Endomembrane traffic in eukaryotic cells functions partially as a means of communication; delivery of membrane in one direction has to be balanced with a reduction at the other end. This effect is typically the case during the defence against pathogens. To combat pathogens, cellular growth and differentiation are supp...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erx363
更新日期:2017-12-18 00:00:00
abstract::Plants have evolved elaborate mechanisms for sensing and responding to sub-optimal environmental conditions. Abiotic stresses caused by these conditions trigger a wide range of local and long-distance signals which must be co-ordinated and integrated into whole-plant processes, such as development, in order for the pl...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv172
更新日期:2015-08-01 00:00:00
abstract::Plant species capable of hyper-accumulating heavy metals are of considerable interest for phytoremediation, and differ in their ability to accumulate metals from the environment. This work aims to examine (i) arsenic accumulation in three fern species [Chinese brake fern (Pteris vittata L.), slender brake fern (Pteris...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri134
更新日期:2005-05-01 00:00:00
abstract::Tomato, melon, grape, peach, and strawberry primarily accumulate soluble sugars during fruit development. In contrast, kiwifruit (Actinidia Lindl. spp.) and banana store a large amount of starch that is released as soluble sugars only after the fruit has reached maturity. By integrating metabolites measured by gas chr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert293
更新日期:2013-11-01 00:00:00
abstract::Ureides dramatically accumulate in shoots of N(2)-fixing soybean (Glycine max L. Merr.) under water deficit and this accumulation is higher in cultivars that have N(2) fixation that is sensitive to water deficit. One possible explanation is that ureide accumulation is associated with a feedback inhibition of nitrogena...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Visual symptoms of the onset of senescence in Sandersonia aurantiaca flowers begin with fading of flower colour and wilting of the tissue. When fully senescent, the flowers form a papery shell that remains attached to the plant. The cell walls of these flowers have been examined to determine whether there are wall mod...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.368.513
更新日期:2002-03-01 00:00:00
abstract::This work examines the involvement of haem oxygenase-1 (HO-1) in salicylic acid (SA)-induced alleviation of oxidative stress as a result of cadmium (Cd) stress in alfalfa (Medicago sativa L.) seedling roots. CdCl(2) exposure caused severe growth inhibition and Cd accumulation, which were potentiated by pre-treatment w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers201
更新日期:2012-09-01 00:00:00
abstract::Recent studies have suggested that aluminium (Al) induces programmed cell death (PCD) in plants. To investigate possible mechanisms, fluorescence techniques were used to monitor the behaviour of mitochondria in vivo, as well as the activation of caspase-3-like activity during protoplast PCD induced by Al. A quick burs...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq279
更新日期:2011-01-01 00:00:00
abstract::The present work describes the intrachloroplast localization and the changes that took place in the thylakoid and stroma-located superoxide dismutases (SOD, EC 1.15.1.1) and ascorbate peroxidases (APX, EC 1.11.1.11), in response to long-term NaCl stress in Pisum sativum L. cv. Puget plants. Native PAGE using high chlo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh013
更新日期:2004-01-01 00:00:00
abstract::Intact coconuts were germinated in situ and compared with excised zygotic embryos germinated in vitro. The growth of the embryonic tissue and their fatty acid compositions were measured. Haustoria, plumules and radicles of coconuts germinated in situ grew continuously and proportionately throughout the 120 d experimen...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.358.933
更新日期:2001-05-01 00:00:00
abstract::Nitrogen (N), as an essential element in amino acids, nucleotides, and proteins, is a key factor in plant growth and development. Omics approaches such as metabolomics and transcriptomics have become a promising way to inspect complex network interactions in N metabolism and can be used for monitoring the uptake and r...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru322
更新日期:2014-10-01 00:00:00
abstract::Plants are known to produce NO via the reduction of nitrite. Oxidative NO production in plants has been considered only with respect to a nitric oxide synthase (NOS). Here it is shown that tobacco cell suspensions emitted NO when hydroxylamine (HA) or salicylhydroxamate (SHAM), a frequently used AOX inhibitor, was add...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp077
更新日期:2009-01-01 00:00:00
abstract::Leaf dry mass per area (LMA) is a composite parameter relating to a suite of structural traits that have the potential to influence photosynthesis. However, the extent to which each of these traits contributes to variation in LMA and photosynthetic rates is not well understood, especially at the high end of the LMA sp...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq128
更新日期:2010-06-01 00:00:00
abstract::Sorghum is an important source of food, feed, and biofuel, especially in the semi-arid tropics because this cereal is well adapted to harsh, drought-prone environments. Post-flowering drought adaptation in sorghum is associated with the stay-green phenotype. Alleles that contribute to this complex trait have been mapp...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl225
更新日期:2007-01-01 00:00:00
abstract::During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain filling, parallel trans...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru492
更新日期:2015-03-01 00:00:00
abstract::Inflorescence stalks produced the highest amount of ethylene in response to IAA as compared with other plant parts tested. Leaf age had an effect on IAA-induced ethylene with the youngest leaves showing the greatest stimulation. The highest amount of IAA-induced ethylene was produced in the root or inflorescence tip w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern159
更新日期:2008-01-01 00:00:00
abstract::The endosperm is an essential part of the seed which sustains embryo development and contains storage reserves. Endosperm development involves a series of nuclear divisions in the absence of cytokinesis. In this study, it is shown that a mutant Arabidopsis plant carrying the disrupted At4g24900 gene exhibits severe de...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers243
更新日期:2012-10-01 00:00:00
abstract::Nitrogen-fixing bacteria have been isolated from sugarcane in an endophytic and beneficial interaction that promotes plant growth. In this work, for the first time, the involvement of ethylene signalling in this interaction was investigated by molecular characterizing members of this pathway in sugarcane. The expressi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl242
更新日期:2007-01-01 00:00:00