当前位置： SCI文献检索 > JOURNAL OF EXPERIMENTAL BOTANY期刊下所有文献 > Mechanistic study of mitochondria-dependent programmed cell death induced by aluminium phytotoxicity using fluorescence techniques.

Mechanistic study of mitochondria-dependent programmed cell death induced by aluminium phytotoxicity using fluorescence techniques.

Abstract：

:Recent studies have suggested that aluminium (Al) induces programmed cell death (PCD) in plants. To investigate possible mechanisms, fluorescence techniques were used to monitor the behaviour of mitochondria in vivo, as well as the activation of caspase-3-like activity during protoplast PCD induced by Al. A quick burst of mitochondrial reactive oxygen species (ROS) was detected in Al-treated protoplasts. The mitochondrial swelling and mitochondrial transmembrane potential (MTP) loss occurred prior to cell death. Pre-incubation with ascorbic acid (AsA, antioxidant molecule) retarded mitochondrial swelling and MTP loss. The real-time detection of caspase-3-like activation was achieved by measuring the degree of fluorescence resonance energy transfer (FRET). At 30 min after exposure to Al, caspase-3-like protease activation, indicated by the decrease in the FRET ratio, occurred, taking about 1 h to reach completion in single living protoplasts. The mitochondrial permeability transition pore (MPTP) inhibitor, cyclosporine (CsA) gave significant protection against MTP loss and subsequent caspase-3-like activation. Our data also showed that Al-induced mitochondrial ROS possibly originated from complex I and III damage in the respiratory chain through the interaction between Al and iron-sulphur (Fe-S) protein. Alternative oxidase (AOX), the unique respiratory terminal oxidase in plants, was demonstrated to play protective roles in Al-induced protoplast death. Our results showed that mitochondrial swelling and MTP loss, as well as the generation of mitochondrial ROS play important roles in Al-induced caspase-3-like activation and PCD, which provided new insight into the signalling cascades that modulate Al phytotoxicity mechanism.

journal_name

J Exp Bot

journal_title

Journal of experimental botany

authors

Li Z,Xing D

doi

10.1093/jxb/erq279

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

331-43

issue

1

eissn

0022-0957

issn

1460-2431

pii

erq279

journal_volume

62

pub_type

杂志文章

相关文献

JOURNAL OF EXPERIMENTAL BOTANY文献大全
  • Regulation of mitochondrial calcium in plants versus animals.

    abstract：:Ca(2+) acts as an important cellular second messenger in eukaryotes. In both plants and animals, a wide variety of environmental and developmental stimuli trigger Ca(2+) transients of a specific signature that can modulate gene expression and metabolism. In animals, mitochondrial energy metabolism has long been consid...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章,评审

    doi：10.1093/jxb/erw100

    authors： Wagner S,De Bortoli S,Schwarzländer M,Szabò I

    更新日期：2016-06-01 00:00:00

  • Inhibition of nitrate influx by glutamine in Lolium perenne depends upon the contribution of the HATS to the total influx.

    abstract：:Plants of Lolium perenne L. were grown in sterile solution culture supplied with 2 mol m(-3) nitrogen as either nitrate or ammonium. Glutamine at 5 mol m(-3) was added to the nutrient solution of half the plants for 24 h. Root nitrate influx (at external nitrate concentrations 0-2000 mmol m(-3)) and amino acid concent...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erh066

    authors： Thornton B

    更新日期：2004-03-01 00:00:00

  • Cell sampling and analysis (SiCSA): metabolites measured at single cell resolution.

    abstract：:By using a fine oil-filled glass microcapillary mounted on a micromanipulator, the solutes of individual plant cells can be sampled. These samples can then be analysed using a range of physical and chemical methods. Hydrostatic pressure (cell pressure probe), osmotic pressure (picolitre osmometer), organic solutes (en...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章,评审

    doi：

    authors： Tomos AD,Sharrock RA

    更新日期：2001-04-01 00:00:00

  • A genetic relationship between nitrogen use efficiency and seedling root traits in maize as revealed by QTL analysis.

    abstract：:That root system architecture (RSA) has an essential role in nitrogen acquisition is expected in maize, but the genetic relationship between RSA and nitrogen use efficiency (NUE) traits remains to be elucidated. Here, the genetic basis of RSA and NUE traits was investigated in maize using a recombination inbred line p...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erv127

    authors： Li P,Chen F,Cai H,Liu J,Pan Q,Liu Z,Gu R,Mi G,Zhang F,Yuan L

    更新日期：2015-06-01 00:00:00

  • CEP3 levels affect starvation-related growth responses of the primary root.

    abstract：:CEPs (C-TERMINALLY ENCODED PEPTIDEs) inhibit Arabidopsis primary root growth by unknown mechanisms. We investigated how CEP3 levels control primary root growth. CEP3 peptide application decreased cell division, S-phase cell number, root meristematic cell number, and meristem zone (MZ) size in a dose- and CEP RECEPTOR1...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erz270

    authors： Delay C,Chapman K,Taleski M,Wang Y,Tyagi S,Xiong Y,Imin N,Djordjevic MA

    更新日期：2019-09-24 00:00:00

  • A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].

    abstract：:Here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/err140

    authors： Zeng Y,Pan Z,Ding Y,Zhu A,Cao H,Xu Q,Deng X

    更新日期：2011-11-01 00:00:00

  • A model for intracellular movement of Cauliflower mosaic virus: the concept of the mobile virion factory.

    abstract：:The genomes of many plant viruses have a coding capacity limited to <10 proteins, yet it is becoming increasingly clear that individual plant virus proteins may interact with several targets in the host for establishment of infection. As new functions are uncovered for individual viral proteins, virologists have reali...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章,评审

    doi：10.1093/jxb/erv520

    authors： Schoelz JE,Angel CA,Nelson RS,Leisner SM

    更新日期：2016-03-01 00:00:00

  • Transcriptome sequencing and metabolite analysis reveals the role of delphinidin metabolism in flower colour in grape hyacinth.

    abstract：:Grape hyacinth (Muscari) is an important ornamental bulbous plant with an extraordinary blue colour. Muscari armeniacum, whose flowers can be naturally white, provides an opportunity to unravel the complex metabolic networks underlying certain biochemical traits, especially colour. A blue flower cDNA library of M. arm...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/eru168

    authors： Lou Q,Liu Y,Qi Y,Jiao S,Tian F,Jiang L,Wang Y

    更新日期：2014-07-01 00:00:00

  • Dying to live: cell elimination as a developmental strategy in angiosperm seeds.

    abstract：:The complete elimination of unwanted cells during development is a repeated theme in both multicellular animals and in plants. In plants, such events have been extensively studied and reviewed in terms of their molecular regulation, of marker genes and proteins expressed, and in terms of cellular changes associated wi...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erw364

    authors： Ingram GC

    更新日期：2017-02-01 00:00:00

  • Resurrection plants optimize photosynthesis despite very thick cell walls by means of chloroplast distribution.

    abstract：:Resurrection plants are vascular species able to sustain extreme desiccation in their vegetative tissues. Despite its potential interest, the role of leaf anatomy in CO2 diffusion and photosynthesis under non-stressed conditions has not been explored in these species. Net CO2 assimilation (An) and its underlying diffu...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erab022

    authors： Nadal M,Perera-Castro AV,Gulías J,Farrant JM,Flexas J

    更新日期：2021-01-23 00:00:00

  • Transcription factor TCP20 regulates peach bud endodormancy by inhibiting DAM5/DAM6 and interacting with ABF2.

    abstract：:The dormancy-associated MADS-box (DAM) genes PpDAM5 and PpDAM6 have been shown to play important roles in bud endodormancy; however, their molecular regulatory mechanism in peach is unclear. In this study, by use of yeast one-hybrid screening, we isolated a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR transc...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erz516

    authors： Wang Q,Xu G,Zhao X,Zhang Z,Wang X,Liu X,Xiao W,Fu X,Chen X,Gao D,Li D,Li L

    更新日期：2020-02-19 00:00:00

  • Down-regulation of a LBD-like gene, OsIG1, leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice.

    abstract：:The indeterminate gametophyte1 (ig1) mutation was first characterized to modulate female gametophyte development in maize (Zea mays). However, the function of its rice orthologue, OsIG1, remains unknown. For this, we first analysed OsIG1 localization from differential tissues in rice. Real-time quantitative PCR (qRT-P...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/eru396

    authors： Zhang J,Tang W,Huang Y,Niu X,Zhao Y,Han Y,Liu Y

    更新日期：2015-01-01 00:00:00

  • Effects of temperature on the cuticular transpiration barrier of two desert plants with water-spender and water-saver strategies.

    abstract：:The efficacy of the cuticular transpiration barrier and its resistance to elevated temperatures are significantly higher in a typical water-saver than in a water-spender plant growing in hot desert. ...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erz018

    authors： Bueno A,Alfarhan A,Arand K,Burghardt M,Deininger AC,Hedrich R,Leide J,Seufert P,Staiger S,Riederer M

    更新日期：2019-03-11 00:00:00

  • The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato.

    abstract：:Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signalling network for improving productivity in crop species. This first requires identifying components of the conserved network and their function in...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/eraa495

    authors： Mori K,Lemaire-Chamley M,Jorly J,Carrari F,Conte M,Asamizu E,Mizoguchi T,Ezura H,Rothan C

    更新日期：2020-10-24 00:00:00

  • Capture and use of solar radiation, water, and nitrogen by sugar beet (Beta vulgaris L.).

    abstract：:Sugar beet is spring-sown for sugar production in most sugar beet-growing countries. It is grown as a vegetative crop and it accumulates yield (sugar) from very early in its growth cycle. As long as the sugar beet plants do not flower, the sugar accumulation period is indefinite and yield continues to increase. This p...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章,评审

    doi：10.1093/jxb/erp110

    authors： Jaggard KW,Qi A,Ober ES

    更新日期：2009-01-01 00:00:00

  • Photo-oxidative stress markers as a measure of abiotic stress-induced leaf senescence: advantages and limitations.

    abstract：:Inside chloroplasts, several abiotic stresses (including drought, high light, salinity, or extreme temperatures) induce a reduction in CO2 assimilation rates with a consequent increase in reactive oxygen species (ROS) production, ultimately leading to leaf senescence and yield loss. Photo-oxidation processes should th...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章,评审

    doi：10.1093/jxb/eru086

    authors： Pintó-Marijuan M,Munné-Bosch S

    更新日期：2014-07-01 00:00:00

  • Xylem tension affects growth-induced water potential and daily elongation of maize leaves.

    abstract：:Diurnal rates of leaf elongation vary in maize (Zea mays L.) and are characterized by a decline each afternoon. The cause of the afternoon decline was investigated. When the atmospheric environment was held constant in a controlled environment, and water and nutrients were adequately supplied to the soil or the roots ...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erm371

    authors： Tang AC,Boyer JS

    更新日期：2008-01-01 00:00:00

  • Root cap removal increases root penetration resistance in maize (Zea mays L).

    abstract：:The root cap assists the passage of the root through soil by means of its slimy mucilage secretion and by the sloughing of its outer cells. The root penetration resistance of decapped primary roots of maize (Zea mays L. cv. Mephisto) was compared with that of intact roots in loose (dry bulk density 1.0 g cm-3; penetra...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erg226

    authors： Iijima M,Higuchi T,Barlow PW,Bengough AG

    更新日期：2003-09-01 00:00:00

  • Transfer of a starch phenotype from wild wheat to bread wheat by deletion of a locus controlling B-type starch granule content.

    abstract：:Our previous genetic analysis of a tetraploid wild wheat species, Aegilops peregrina, predicted that a single gene per haploid genome, Bgc-1, controls B-type starch granule content in the grain. To test whether bread wheat (Triticum aestivum L.) has orthologous Bgc-1 loci, we screened a population of γ-irradiated brea...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erx349

    authors： Chia T,Adamski NM,Saccomanno B,Greenland A,Nash A,Uauy C,Trafford K

    更新日期：2017-11-28 00:00:00

  • Senescence-inducible cell wall and intracellular purple acid phosphatases: implications for phosphorus remobilization in Hakea prostrata (Proteaceae) and Arabidopsis thaliana (Brassicaceae).

    abstract：:Despite its agronomic importance, the metabolic networks mediating phosphorus (P) remobilization during plant senescence are poorly understood. Highly efficient P remobilization (~85%) from senescing leaves and proteoid roots of harsh hakea (Hakea prostrata), a native 'extremophile' plant of south-western Australia, w...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/eru348

    authors： Shane MW,Stigter K,Fedosejevs ET,Plaxton WC

    更新日期：2014-11-01 00:00:00

  • Strigolactone regulation of shoot branching in chrysanthemum (Dendranthema grandiflorum).

    abstract：:Previous studies of highly branched mutants in pea (rms1-rms5), Arabidopsis thaliana (max1-max4), petunia (dad1-dad3), and rice (d3, d10, htd1/d17, d14, d27) identified strigolactones or their derivates (SLs), as shoot branching inhibitors. This recent discovery offers the possibility of using SLs to regulate branchin...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erq133

    authors： Liang J,Zhao L,Challis R,Leyser O

    更新日期：2010-06-01 00:00:00

  • Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings.

    abstract：:The majority of proteins in eukaryotic cells are modified according to highly regulated mechanisms to fulfill specific functions and to achieve localization, stability, and transport. Protein ubiquitination is one of the major post-translational modifications occurring in eukaryotic cells. To obtain the proteomic data...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erp134

    authors： Igawa T,Fujiwara M,Takahashi H,Sawasaki T,Endo Y,Seki M,Shinozaki K,Fukao Y,Yanagawa Y

    更新日期：2009-01-01 00:00:00

  • Direct in vivo evidence of immense stem water exploitation in irrigated date palms.

    abstract：:During the summer, evaporative demand at midday often exceeds the transport capacity of most desert plants. However, date palms maintain their ecological dominance with sustained and uniquely high rates of transpiration. This high rate of flow cannot be attributed to soil water supply alone. In order to quantify intra...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/eru421

    authors： Sperling O,Shapira O,Schwartz A,Lazarovitch N

    更新日期：2015-01-01 00:00:00

  • A comparison of manual and automated systems for soil CO2 flux measurements: trade-offs between spatial and temporal resolution.

    abstract：:Soil respiration is affected by distributions of roots and soil carbon substrates and by temperature and soil water content, all of which vary spatially and temporally. The objective of this paper was to compare a manual system for measuring soil respiration in a temperate forest, which had a greater spatial distribut...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erg121

    authors： Savage KE,Davidson EA

    更新日期：2003-03-01 00:00:00

  • Cloning of the PpNHAD1 transporter of Physcomitrella patens, a chloroplast transporter highly conserved in photosynthetic eukaryotic organisms.

    abstract：:A cDNA that encodes a transporter from the NHAD family was identified in Physcomitrella patens. Computer-based searches using the amino acid sequence of PpNHAD1 revealed that, in addition to being expressed in flowering plants, highly conserved transporters of this family are expressed in red algae, green algae, mosse...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erm094

    authors： Barrero-Gil J,Rodríguez-Navarro A,Benito B

    更新日期：2007-01-01 00:00:00

  • Combining field performance with controlled environment plant imaging to identify the genetic control of growth and transpiration underlying yield response to water-deficit stress in wheat.

    abstract：:Crop yield in low-rainfall environments is a complex trait under multigenic control that shows significant genotype×environment (G×E) interaction. One way to understand and track this trait is to link physiological studies to genetics by using imaging platforms to phenotype large segregating populations. A wheat popul...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erv320

    authors： Parent B,Shahinnia F,Maphosa L,Berger B,Rabie H,Chalmers K,Kovalchuk A,Langridge P,Fleury D

    更新日期：2015-09-01 00:00:00

  • ZmIBH1-1 regulates plant architecture in maize.

    abstract：:Leaf angle (LA) is a critical agronomic trait in maize, with more upright leaves allowing higher planting density, leading to more efficient light capture and higher yields. A few genes responsible for variation in LA have been identified by map-based cloning. In this study, we cloned maize ZmIBH1-1, which encodes a b...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/eraa052

    authors： Cao Y,Zeng H,Ku L,Ren Z,Han Y,Su H,Dou D,Liu H,Dong Y,Zhu F,Li T,Zhao Q,Chen Y

    更新日期：2020-05-30 00:00:00

  • Role of early signalling events in plant-insect interactions.

    abstract：:The response of plants to the stress caused by herbivores involves several different defence mechanisms. These responses begin at the plant cell plasma membrane, where insect herbivores interact physically by causing mechanical damage and chemically by introducing elicitors or by triggering plant-derived signalling mo...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章,评审

    doi：10.1093/jxb/eru480

    authors： Zebelo SA,Maffei ME

    更新日期：2015-02-01 00:00:00

  • News from the PUB: plant U-box type E3 ubiquitin ligases.

    abstract：:Plant U-box type E3 ubiquitin ligases (PUBs) are well known for their functions in a variety of stress responses, including immune responses and the adaptation to abiotic stresses. First linked to pollen self-incompatibility, their repertoire of roles has grown to encompass also the regulation of developmental process...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章,评审

    doi：10.1093/jxb/erx411

    authors： Trujillo M

    更新日期：2018-01-23 00:00:00

  • Sorghum stay-green QTL individually reduce post-flowering drought-induced leaf senescence.

    abstract：:Sorghum is an important source of food, feed, and biofuel, especially in the semi-arid tropics because this cereal is well adapted to harsh, drought-prone environments. Post-flowering drought adaptation in sorghum is associated with the stay-green phenotype. Alleles that contribute to this complex trait have been mapp...

    journal_title：Journal of experimental botany

    pub_type： 杂志文章

    doi：10.1093/jxb/erl225

    authors： Harris K,Subudhi PK,Borrell A,Jordan D,Rosenow D,Nguyen H,Klein P,Klein R,Mullet J

    更新日期：2007-01-01 00:00:00