A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].

Abstract:

:Here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. Based on the predicted functions of these identified plastid proteins, a large proportion (∼60%) of the chromoplast proteome of sweet orange is constituted by proteins involved in carbohydrate metabolism, amino acid/protein synthesis, and secondary metabolism. Of note, HDS (hydroxymethylbutenyl 4-diphosphate synthase), PAP (plastid-lipid-associated protein), and psHSPs (plastid small heat shock proteins) involved in the synthesis or storage of carotenoid and stress response are among the most abundant proteins identified. A comparison of chromoplast proteomes between sweet orange and tomato suggested a high level of conservation in a broad range of metabolic pathways. However, the citrus chromoplast was characterized by more extensive carotenoid synthesis, extensive amino acid synthesis without nitrogen assimilation, and evidence for lipid metabolism concerning jasmonic acid synthesis. In conclusion, this study provides an insight into the major metabolic pathways as well as some unique characteristics of the sweet orange chromoplasts at the whole proteome level.

journal_name

J Exp Bot

authors

Zeng Y,Pan Z,Ding Y,Zhu A,Cao H,Xu Q,Deng X

doi

10.1093/jxb/err140

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

5297-309

issue

15

eissn

0022-0957

issn

1460-2431

pii

err140

journal_volume

62

pub_type

杂志文章
  • Recalculating growth and defense strategies under competition: key roles of photoreceptors and jasmonates.

    abstract::The growth-defense trade-off in plant biology has gained enormous traction in the last two decades, highlighting the importance of understanding how plants deal with two of the greatest challenges for their survival and reproduction. It has been well established that in response to competition signals perceived by inf...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz237

    authors: Ballaré CL,Austin AT

    更新日期:2019-07-05 00:00:00

  • Transcriptional and biochemical analyses of gibberellin expression and content in germinated barley grain.

    abstract::Mobilization of reserves in germinated cereal grains is critical for early seedling vigour, global crop productivity, and hence food security. Gibberellins (GAs) are central to this process. We have developed a spatio-temporal model that describes the multifaceted mechanisms of GA regulation in germinated barley grain...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz546

    authors: Betts NS,Dockter C,Berkowitz O,Collins HM,Hooi M,Lu Q,Burton RA,Bulone V,Skadhauge B,Whelan J,Fincher GB

    更新日期:2020-03-25 00:00:00

  • Update on the genetic control of flowering in garden pea.

    abstract::The garden pea has been a model for the genetics of flowering for several decades and numerous flowering loci have been identified, but until recently little was known about the molecular nature of these loci. This paper presents an update on recent work on the molecular genetics of flowering in pea, outlining progres...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erp120

    authors: Weller JL,Hecht V,Liew LC,Sussmilch FC,Wenden B,Knowles CL,Vander Schoor JK

    更新日期:2009-01-01 00:00:00

  • Light quality modulates metabolic synchronization over the diel phases of crassulacean acid metabolism.

    abstract::Temporal compartmentation of carboxylation processes is a defining feature of crassulacean acid metabolism and involves circadian control of key metabolic and transport steps that regulate the supply and demand for carbon over a 24h cycle. Recent insights on the molecular workings of the circadian clock and its connec...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru185

    authors: Ceusters J,Borland AM,Taybi T,Frans M,Godts C,De Proft MP

    更新日期:2014-07-01 00:00:00

  • A 9 bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses.

    abstract::In rice, the class I small heat shock protein (sHSP-CI) genes were found to be selectively induced by L-azetidine-2-carboxylic acid (AZC) on chromosome 3 but not chromosome 1. Here it is shown that a novel cis-responsive element contributed to the differential regulation. By serial deletion and computational analysis,...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq230

    authors: Guan JC,Yeh CH,Lin YP,Ke YT,Chen MT,You JW,Liu YH,Lu CA,Wu SJ,Lin CY

    更新日期:2010-10-01 00:00:00

  • News from the PUB: plant U-box type E3 ubiquitin ligases.

    abstract::Plant U-box type E3 ubiquitin ligases (PUBs) are well known for their functions in a variety of stress responses, including immune responses and the adaptation to abiotic stresses. First linked to pollen self-incompatibility, their repertoire of roles has grown to encompass also the regulation of developmental process...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erx411

    authors: Trujillo M

    更新日期:2018-01-23 00:00:00

  • Elevated sucrose-phosphate synthase activity in transgenic tobacco sustains photosynthesis in older leaves and alters development.

    abstract::Constitutive over-expression of a maize sucrose-phosphate synthase (SPS) gene in tobacco (Nicotiana tabacum) had major effects on leaf carbohydrate budgets with consequences for whole plant development. Transgenic tobacco plants flowered earlier and had greater flower numbers than wild-type plants. These changes were ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg196

    authors: Baxter CJ,Foyer CH,Turner J,Rolfe SA,Quick WP

    更新日期:2003-08-01 00:00:00

  • Osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to Botrytis cinerea.

    abstract::Abiotic factors inducing osmotic stress can influence the plant immune response and resistance to pathogen infections. In this study, the effect of polyethylene glycol (PEG)- and sucrose-induced osmotic stress on polyamine (PA) homeostasis and the basal immune response in grapevine plantlets before and after Botrytis ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert351

    authors: Hatmi S,Trotel-Aziz P,Villaume S,Couderchet M,Clément C,Aziz A

    更新日期:2014-01-01 00:00:00

  • Genetic engineering of rice capable of synthesizing fructans and enhancing chilling tolerance.

    abstract::Fructans are water-soluble fructose oligomers and polymers that are based on sucrose, and have been implicated in protecting plants against water stress. Rice (Oryza sativa L.) is highly sensitive to chilling temperatures, and is not able to synthesize fructans. Two wheat fructan-synthesizing enzymes, sucrose:sucrose ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm367

    authors: Kawakami A,Sato Y,Yoshida M

    更新日期:2008-01-01 00:00:00

  • Ethylene is required for both the initiation and progression of softening in pear (Pyrus communis L.) fruit.

    abstract::In order to investigate the physiological role of ethylene in the initiation and subsequent progression of softening, pear fruit were treated with propylene, an analogue of ethylene or 1-methylcyclopropene (1-MCP), a gaseous inhibitor of ethylene action at the preclimacteric or ripening stages. The propylene treatment...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg073

    authors: Hiwasa K,Kinugasa Y,Amano S,Hashimoto A,Nakano R,Inaba A,Kubo Y

    更新日期:2003-02-01 00:00:00

  • Calcium channel CNGC19 mediates basal defense signaling to regulate colonization by Piriformospora indica in Arabidopsis roots.

    abstract::The activation of calcium signaling is a crucial event for perceiving environmental stress. Colonization by Piriformospora indica, a growth-promoting root endosymbiont, activates cytosolic Ca2+ in Arabidopsis roots. In this study, we examined the role and functional relevance of calcium channels responsible for Ca2+ f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa028

    authors: Jogawat A,Meena MK,Kundu A,Varma M,Vadassery J

    更新日期:2020-05-09 00:00:00

  • An AGAMOUS MADS-box protein, SlMBP3, regulates the speed of placenta liquefaction and controls seed formation in tomato.

    abstract::AGAMOUS (AG) MADS-box transcription factors have been shown to play crucial roles in floral organ and fruit development in angiosperms. Here, we isolated a tomato (Solanum lycopersicum) AG MADS-box gene SlMBP3 and found that it is preferentially expressed in flowers and during early fruit developmental stages in the w...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery418

    authors: Zhang J,Wang Y,Naeem M,Zhu M,Li J,Yu X,Hu Z,Chen G

    更新日期:2019-02-05 00:00:00

  • Independent induction of two blue light-dependent monovalent anion transport systems in the plasma membrane of Monoraphidium braunii.

    abstract::In the plasma membrane of the green alga Monoraphidium braunii there are at least two monovalent anion transport systems. One of them is specific for bicarbonate. This transport system is activated by blue light and its induction is triggered by a decrease in the external CO2 concentration. The second transport system...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf056

    authors: Mora C,Witt FG,Aparicio PJ,Quiñones MA

    更新日期:2002-09-01 00:00:00

  • The suffulta mutation in tomato reveals a novel method of plastid replication during fruit ripening.

    abstract::Mutant alleles at the suffulta locus of tomato dramatically affect the pattern of plastid division throughout the plant, resulting in few, greatly enlarged chloroplasts in leaf and stem cells. suffulta plants are compromised in growth and have distinctly pale stems. The green developing tomato fruit are generally pale...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj144

    authors: Forth D,Pyke KA

    更新日期:2006-01-01 00:00:00

  • Reduced expression of top1beta gene induces programmed cell death and alters ascorbate metabolism in Daucus carota cultured cells.

    abstract::Topoisomerase I (topo I) is a nuclear enzyme which plays a fundamental role in several pathways involving changes in DNA topology. The topo I-mediated reaction is accomplished by the transient covalent binding of the enzyme to DNA (topo I-DNA complex). Stabilization of the topo I-DNA complex, leading to irreversible d...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj194

    authors: Locato V,Balestrazzi A,De Gara L,Carbonera D

    更新日期:2006-01-01 00:00:00

  • Darwin's foundation for investigating self-incompatibility and the progress toward a physiological model for S-RNase-based SI.

    abstract::Charles Darwin made extensive observations of the pollination biology of a wide variety of plants. He carefully documented the consequences of self-pollination and described species that were self-sterile but that could easily be crossed with other plants of the same species. He believed that compatibility was control...

    journal_title:Journal of experimental botany

    pub_type: 历史文章,杂志文章,评审

    doi:10.1093/jxb/erp024

    authors: McClure B

    更新日期:2009-01-01 00:00:00

  • Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves.

    abstract::The influence of reduced glycine decarboxylase complex (GDC) activity on leaf atmosphere CO2 and 13CO2 exchange was tested in transgenic Oryza sativa with the GDC H-subunit knocked down in leaf mesophyll cells. Leaf measurements on transgenic gdch knockdown and wild-type plants were carried out in the light under phot...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz083

    authors: Giuliani R,Karki S,Covshoff S,Lin HC,Coe RA,Koteyeva NK,Quick WP,Von Caemmerer S,Furbank RT,Hibberd JM,Edwards GE,Cousins AB

    更新日期:2019-05-09 00:00:00

  • Foxtail mosaic virus-induced flowering assays in monocot crops.

    abstract::Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and wood...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa080

    authors: Yuan C,Li H,Qin C,Zhang X,Chen Q,Zhang P,Xu X,He M,Zhang X,Tör M,Xue D,Wang H,Jackson S,He Y,Liu Y,Shi N,Hong Y

    更新日期:2020-05-30 00:00:00

  • Molecular diversity of bacterial production of the climate-changing gas, dimethyl sulphide, a molecule that impinges on local and global symbioses.

    abstract::This paper describes the ddd genes that are involved in the production of the gas dimethyl sulphide from the substrate dimethylsulphoniopropionate (DMSP), an abundant molecule that is a stress protectant in many marine algae and a few genera of angiosperms. What is known of the arrangement of the ddd genes in differen...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erm264

    authors: Johnston AW,Todd JD,Sun L,Nikolaidou-Katsaridou MN,Curson AR,Rogers R

    更新日期:2008-01-01 00:00:00

  • Expression of unprocessed glutelin precursor alters polymerization without affecting trafficking and accumulation.

    abstract::Rice glutelin is synthesized as a precursor in the endosperm endoplasmic reticulum and then deposited within the protein storage vacuole protein body-II (PB-II) as an aggregate, with a high degree of polymerized higher-order structure comprising mature acidic and basic subunits after post-translation processing cleava...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp187

    authors: Wakasa Y,Yang L,Hirose S,Takaiwa F

    更新日期:2009-01-01 00:00:00

  • A comparative study of the expression of serine proteinases in quiescent seeds and in developing Canavalia ensiformis plants.

    abstract::An alkaline proteinase activity is present in quiescent seeds and up to the 24th day of development of Canavalia ensiformis DC (L.) plants. By a simple protocol consisting of cation exchange chromatography, followed by an anion exchange column, a serine proteinase (Q-SP) was purified to homogeneity from quiescent seed...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl223

    authors: Demartini DR,Wlodawer A,Carlini CR

    更新日期:2007-01-01 00:00:00

  • MGOUN3: evidence for chromatin-mediated regulation of FLC expression.

    abstract::The MGOUN3(MGO3)/BRUSHY1(BRU1)/TONSOKU(TSK) gene of Arabidopsis thaliana encodes a nuclear leucine-glycine-asparagine (LGN) domain protein that may be implicated in chromatin dynamics and genome maintenance. Mutants with defects in MGO3 display a fasciated stem and disorganized meristem structures. The transition to f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj169

    authors: Guyomarc'h S,Benhamed M,Lemonnier G,Renou JP,Zhou DX,Delarue M

    更新日期:2006-01-01 00:00:00

  • Alq mutation increases fruit set rate and allows the maintenance of fruit yield under moderate saline conditions.

    abstract::Arlequin (Alq) is a gain-of-function mutant whose most relevant feature is that sepals are able to become fruit-like organs due to the ectopic expression of the ALQ-TAGL1 gene. The role of this gene in tomato fruit ripening was previously demonstrated. To discover new functional roles for ALQ-TAGL1, and most particula...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz342

    authors: Ribelles C,García-Sogo B,Yuste-Lisbona FJ,Atarés A,Castañeda L,Capel C,Lozano R,Moreno V,Pineda B

    更新日期:2019-10-24 00:00:00

  • Resurrection plants optimize photosynthesis despite very thick cell walls by means of chloroplast distribution.

    abstract::Resurrection plants are vascular species able to sustain extreme desiccation in their vegetative tissues. Despite its potential interest, the role of leaf anatomy in CO2 diffusion and photosynthesis under non-stressed conditions has not been explored in these species. Net CO2 assimilation (An) and its underlying diffu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erab022

    authors: Nadal M,Perera-Castro AV,Gulías J,Farrant JM,Flexas J

    更新日期:2021-01-23 00:00:00

  • Overexpression of HvAKT1 improves drought tolerance in barley by regulating root ion homeostasis and ROS and NO signaling.

    abstract::Potassium (K+) is the major cationic inorganic nutrient utilized for osmotic regulation, cell growth, and enzyme activation in plants. Inwardly rectifying K+ channel 1 (AKT1) is the primary channel for root K+ uptake in plants, but the function of HvAKT1 in barley plants under drought stress has not been fully elucida...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa354

    authors: Feng X,Liu W,Cao F,Wang Y,Zhang G,Chen ZH,Wu F

    更新日期:2020-10-22 00:00:00

  • Critical assessment of assembly strategies for non-model species mRNA-Seq data and application of next-generation sequencing to the comparison of C(3) and C(4) species.

    abstract::Next-generation sequencing enables the study of species without a sequenced genome at the 'omics' level. Custom transcriptome databases are generated and global expression profiles can be compared. However, the assembly of transcriptome sequence reads into contigs remains a daunting task. In this study, five different...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err029

    authors: Bräutigam A,Mullick T,Schliesky S,Weber AP

    更新日期:2011-05-01 00:00:00

  • Multifaceted roles of nitric oxide in tomato fruit ripening: NO-induced metabolic rewiring and consequences for fruit quality traits.

    abstract::Nitric oxide (NO) has been increasingly implicated as part of the ripening regulatory networks in fleshy fruits. However, very little is known about the simultaneous action of NO on the intertwined web of regulatory events and metabolic reactions behind the ripening-related changes in fruit color, taste, aroma and nut...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa526

    authors: Zuccarelli R,Rodríguez-Ruiz M,Lopes-Oliveira PJ,Pascoal GB,Andrade SCS,Furlan CM,Purgatto E,Palma JM,Corpas FJ,Rossi M,Freschi L

    更新日期:2020-11-09 00:00:00

  • Lateral root formation and growth of Arabidopsis is redundantly regulated by cytokinin metabolism and signalling genes.

    abstract::The plant root system is important for the uptake of water and nutrients and the anchoring of plants in the soil. Lateral roots (LRs) contribute considerably to root system architecture. Their post-embryonic formation is regulated by hormones and environmental cues. The hormone cytokinin influences LR formation and gr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert291

    authors: Chang L,Ramireddy E,Schmülling T

    更新日期:2013-11-01 00:00:00

  • Synthetic biology approaches for improving photosynthesis.

    abstract::The phenomenal increase in agricultural yields that we have witnessed in the last century has slowed down as we approach the limits of selective breeding and optimization of cultivation techniques. To support the yield increase required to feed an ever-growing population, we will have to identify new ways to boost the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz029

    authors: Kubis A,Bar-Even A

    更新日期:2019-03-11 00:00:00

  • Ion-specific mechanisms of osmoregulation in bean mesophyll cells.

    abstract::Transient kinetics of net H+, K+, Ca2+, and Cl- fluxes were measured non-invasively, using an ion-selective microelectrode technique, for bean (Vicia faba L.) leaf mesophyll in response to 150 mM mannitol treatment. In a parallel set of experiments, changes in the plasma membrane potential and the total proline conten...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Shabala S,Babourina O,Newman I

    更新日期:2000-07-01 00:00:00