Abstract：
:An alkaline proteinase activity is present in quiescent seeds and up to the 24th day of development of Canavalia ensiformis DC (L.) plants. By a simple protocol consisting of cation exchange chromatography, followed by an anion exchange column, a serine proteinase (Q-SP) was purified to homogeneity from quiescent seeds. Q-SP consists of a 33 kDa chain with an optimum pH between 8.0 and 9.0. Arginine residues at P1 and P2 subsites favour binding to the substrate, as shown by the KM assay with N-alpha-benzoyl-DL-arginine-4-nitroanilide-hydrochloride and N-benzoylcarboxyl-L-arginyl-L-arginine-7-amido-4-methylcoumarin. The same protocol was used for partial purification of benzamidine-sensitive enzymes from the developing plant. On the 7th day, a new benzamidine-sensitive enzyme is synthesized in the seedling, seen as the second active peak appearing in anion exchange chromatography. A benzamidine-sensitive enzyme purified from cotyledons presented a similar gel filtration profile as Q-SP, although it was eluted at different salt concentrations in the anion exchange chromatography. None of the enzymes was inhibited by PMSF, APMSF, or SBTI, but they were inactivated by benzamidine, TLCK, and leupeptin. Q-SP did not cleave in vitro C. ensiformis urease, concanavalin A, or its main storage protein, canavalin. In conclusion, a ubiquitous benzamidine-sensitive proteolytic activity was found in C. ensiformis from quiescent seeds up to 24 d of growth, which apparently is not involved in the hydrolysis of storage proteins and might participate in an as yet unidentified limited proteolysis event.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Demartini DR,Wlodawer A,Carlini CRdoi
10.1093/jxb/erl223subject
Has Abstractpub_date
2007-01-01 00:00:00pages
521-32issue
3eissn
0022-0957issn
1460-2431pii
erl223journal_volume
58pub_type
杂志文章abstract：:Recent studies have suggested that aluminium (Al) induces programmed cell death (PCD) in plants. To investigate possible mechanisms, fluorescence techniques were used to monitor the behaviour of mitochondria in vivo, as well as the activation of caspase-3-like activity during protoplast PCD induced by Al. A quick burs...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erq279
更新日期：2011-01-01 00:00:00
abstract：:The reproductive success of plants largely depends on the correct programming of developmental phase transitions, particularly the shift from vegetative to reproductive growth. The timing of this transition is finely regulated by the integration of an array of environmental and endogenous factors. Nitrogen is the mine...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erx423
更新日期：2018-01-23 00:00:00
abstract：:Many molecular mechanisms that regulate dormancy have been identified individually in controlled laboratory studies. However, little is known about how the seed employs this complex suite of mechanisms during dormancy cycling in the variable environment of the soil seed bank. Nevertheless, this behaviour is essential ...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erw477
更新日期：2017-02-01 00:00:00
abstract：:Mobile transcription factors play many roles in plant development. Here, we compare the use of mobile transcription factors as signals with some canonical signal transduction processes in prokaryotes and eukaryotes. After an initial survey, we focus on the SHORT-ROOT pathway in Arabidopsis roots to show that, despite ...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/eru548
更新日期：2015-02-01 00:00:00
abstract：:Shipping of peaches to distant markets and storage require low temperature; however, cold storage affects fruit quality causing physiological disorders collectively termed 'chilling injury' (CI). In order to ameliorate CI, different strategies have been applied before cold storage; among them heat treatment (HT) has b...
journal_title：Journal of experimental botany
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更新日期：2009-01-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/err326
更新日期：2012-02-01 00:00:00
abstract：:The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxid...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erp256
更新日期：2009-01-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/err088
更新日期：2011-07-01 00:00:00
abstract：:Oilseed rape (Brassica napus L.) is commonly grown for oil or bio-fuel production, while the seed residues can be used for animal feed. It can also be grown as a catch crop because of its efficiency in extracting mineral N from the soil profile. However, the N harvest index is usually low, due in part to a low ability...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eraa504
更新日期：2020-11-27 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/erg087
更新日期：2003-02-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ert488
更新日期：2014-04-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erx195
更新日期：2017-06-15 00:00:00
abstract：:Collections of specimens held by natural history museums are invaluable material for biodiversity inventory and evolutionary studies, with specimens accumulated over 300 years readily available for sampling. Unfortunately, most museum specimens yield low-quality DNA. Recent advances in sequencing technologies, so call...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eru395
更新日期：2014-12-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eraa012
更新日期：2020-03-12 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jexbot/53.369.699
更新日期：2002-04-01 00:00:00
abstract：:Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key enzyme in the fixation of CO(2) in the Calvin cycle of plants. Many genome projects have revealed that bacteria, including Bacillus subtilis, possess genes for proteins that are similar to the large subunit of RuBisCO. These RuBisCO homologues are ca...
journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ern104
更新日期：2008-01-01 00:00:00
abstract：:Individuals, families, networks, and botanic gardens have made records of flowering times of a wide range of plant species over many years. These data can highlight year to year changes in seasonal events (phenology) and those datasets covering long periods draw interest for their perspective on plant responses to cli...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erq165
更新日期：2010-06-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eraa495
更新日期：2020-10-24 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ers231
更新日期：2012-10-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/ery060
更新日期：2018-04-09 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
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更新日期：2017-12-18 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erg003
更新日期：2003-01-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erv057
更新日期：2015-03-01 00:00:00
abstract：:Plant mitochondrial genes contain cis- and trans-group II introns that must be spliced before translation. The mechanism by which these introns are spliced is not well understood. Several families of proteins have been implicated in the intron splicing, of which the pentatricopeptide repeat (PPR) proteins are proposed...
journal_title：Journal of experimental botany
pub_type： 杂志文章
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更新日期：2019-02-05 00:00:00
abstract：:Meiosis is an essential process for sexually reproducing organisms, leading to the formation of specialized generative cells. This review intends to highlight current knowledge of early events during meiosis derived from various model organisms, including plants. It will particularly focus on cis- and trans-requiremen...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erq421
更新日期：2011-03-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/eru130
更新日期：2014-07-01 00:00:00
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journal_title：Journal of experimental botany
pub_type： 杂志文章
doi：10.1093/jxb/ern290
更新日期：2009-01-01 00:00:00
abstract：:The relationship between photosynthetic energy conservation and thermal dissipation of light energy is considered, with emphasis on organisms which tolerate full desiccation without suffering photo-oxidative damage in strong light. As soon as water becomes available to dry poikilohydric organisms, they resume photosyn...
journal_title：Journal of experimental botany
pub_type： 杂志文章,评审
doi：10.1093/jxb/erj104
更新日期：2006-01-01 00:00:00
