Abstract:
:Our previous genetic analysis of a tetraploid wild wheat species, Aegilops peregrina, predicted that a single gene per haploid genome, Bgc-1, controls B-type starch granule content in the grain. To test whether bread wheat (Triticum aestivum L.) has orthologous Bgc-1 loci, we screened a population of γ-irradiated bread wheat cv. Paragon for deletions of the group 4 chromosomes spanning Bgc-1. Suitable deletions, each encompassing ~600-700 genes, were discovered for chromosomes 4A and 4D. These two deletions are predicted to have 240 homoeologous genes in common. In contrast to single deletion mutant plants, double deletion mutants were found to lack B-type starch granules. The B-less grains had normal A-type starch granule morphology, normal overall starch content, and normal grain weight. In addition to variation in starch granule size distribution, the B-less wheat grains differed from controls in grain hardness, starch swelling power, and amylose content. We believe that these B-less wheat plants are the only Triticeae cereals available that combine substantial alterations in starch granule size distribution with minimal impact on starch content.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Chia T,Adamski NM,Saccomanno B,Greenland A,Nash A,Uauy C,Trafford Kdoi
10.1093/jxb/erx349subject
Has Abstractpub_date
2017-11-28 00:00:00pages
5497-5509issue
20eissn
0022-0957issn
1460-2431pii
4583657journal_volume
68pub_type
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