Abstract:
:Proline metabolism is important for environmental responses, plant growth, and development. However, its precise roles in plant abiotic stress tolerance are not well understood. Mutants are valuable for the identification of new genes and for elucidating their roles in physiological mechanisms. We applied a suppressor mutation approach to identify novel genes involved in the regulation of proline metabolism in Arabidopsis. Using the atrzf1 (Arabidopsis thaliana ring zinc finger 1) mutant as a parental line for activation tagging mutagenesis, we selected several mutants with suppressed induction of proline accumulation under dehydration conditions. One of the selected mutants [proline content alterative 22 (pca22)] appeared to have reduced proline contents compared with the atrzf1 mutant under drought stress. Generally, pca22 mutant plants displayed suppressed atrzf1 insensitivity to dehydration and abscisic acid during early seedling growth. Additionally, the pca22 mutant exhibited shorter pollen tube length than wild-type (WT) and atrzf1 plants. Furthermore, PCA22-overexpressing plants were more sensitive to dehydration stress than the WT and RNAi lines. Green fluorescent protein-tagged PCA22 was localized to the cytoplasm of transgenic Arabidopsis cells. Collectively, these results suggest that pca22 acts as dominant suppressor mutant of atrzf1 in the abiotic stress response.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Kim AR,Min JH,Lee KH,Kim CSdoi
10.1093/jxb/erx069subject
Has Abstractpub_date
2017-03-01 00:00:00pages
1797-1809issue
7eissn
0022-0957issn
1460-2431pii
3076470journal_volume
68pub_type
杂志文章abstract::To understand the mechanisms of ion homeostasis in salt-tolerant and salt-sensitive plants, cDNAs for a high-affinity K(+) transporter PhaHKT1 were isolated from salt-sensitive (Utsunomiya) and salt-tolerant (Nanpi, Enchi) reed plants. A cDNA of Utsunomiya (PhaHKT1-u) contained two insertions in the region correspondi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm306
更新日期:2007-01-01 00:00:00
abstract::Oxidative cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the regulation of abscisic acid (ABA) synthesis in higher plants. ABA has been associated with dormancy and flower senescence, while also regulating plant adaptive responses to various environmental stresses....
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err293
更新日期:2012-01-01 00:00:00
abstract::In C(3) plants, diffusion of CO(2) into leaves is restricted by stomata and subsequently by the intercellular airspaces and liquid phase into chloroplasts. While considerable information exists on the effect of environmental conditions on stomatal conductance (g(s)), little is known on whether the mesophyll conductanc...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp035
更新日期:2009-01-01 00:00:00
abstract::The inaccessibility of the zygote and proembryos of angiosperms within the surrounding maternal and filial tissues has hampered studies on early plant embryogenesis. Somatic and gametophytic embryo cultures are often used as alternative systems for molecular and biochemical studies on early embryogenesis, but are not ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm358
更新日期:2008-01-01 00:00:00
abstract::Ca(2+) acts as an important cellular second messenger in eukaryotes. In both plants and animals, a wide variety of environmental and developmental stimuli trigger Ca(2+) transients of a specific signature that can modulate gene expression and metabolism. In animals, mitochondrial energy metabolism has long been consid...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw100
更新日期:2016-06-01 00:00:00
abstract::Brassinosteroids (BRs) are a group of steroid hormones regulating plant growth and development. Since BRs do not undergo transport among plant tissues, their metabolism is tightly regulated by transcription factors (TFs) and feedback loops. BAS1 (CYP734A1, formerly CYP72B1) and SOB7 (CYP72C1) are two BR-inactivating c...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz468
更新日期:2020-01-23 00:00:00
abstract::Rutin is an antioxidant with many interesting pharmacological effects. It can also be found in buckwheat (Fagopyrum esculentum Moench). UV radiation stimulates the activity of enzymes of the phenylpropanoid pathway and there is some evidence that it influences the rutin content in plants. The aim of the present resear...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erf032
更新日期:2002-08-01 00:00:00
abstract::Small signalling peptides have emerged as important cell to cell messengers in plant development and stress responses. However, only a few of the predicted peptides have been functionally characterized. Here, we present functional characterization of two members of the IDA-LIKE (IDL) peptide family in Arabidopsis thal...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx168
更新日期:2017-06-15 00:00:00
abstract::This article reviews the historical development of cytology and cytogenetics in Arabidopsis, and summarizes recent developments in molecular cytogenetics, with special emphasis on meiotic studies. Despite the small genome and small chromosomes of Arabidopsis, considerable progress has been made in developing appropria...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erg034
更新日期:2003-01-01 00:00:00
abstract::Photorespiration is an essential high flux metabolic pathway that is found in all oxygen-producing photosynthetic organisms. It is often viewed as a closed metabolic repair pathway that serves to detoxify 2-phosphoglycolic acid and to recycle carbon to fuel the Calvin-Benson cycle. However, this view is too simplistic...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw145
更新日期:2016-05-01 00:00:00
abstract::Plants generate reactive oxygen species (ROS) as part of their metabolism and in response to various external stress factors, potentially causing significant damage to biomolecules and cell structures. During the course of evolution, plants have adapted to ROS toxicity, and use ROS as signalling messengers that activa...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv044
更新日期:2015-05-01 00:00:00
abstract::Portulacaceae is one of 19 families of terrestrial plants in which species having C(4) photosynthesis have been found. Representative species from major clades of the genus Portulaca were studied to characterize the forms of photosynthesis structurally and biochemically. The species P. amilis, P. grandiflora, P. molok...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq178
更新日期:2010-08-01 00:00:00
abstract::Voltage dependence of ionic currents and ion fluxes in a walled, turgor-regulating cell were measured in Neurospora crassa. The hyphal morphology of the model organism Neurospora simplifies cable analysis of ionic currents to determine current density for quantitative comparisons with ion fluxes. The ion fluxes were m...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm204
更新日期:2007-01-01 00:00:00
abstract::S-RNase based gametophytic self-incompatibility (SI) is a widespread prezygotic reproductive barrier in flowering plants. In the Solanaceae, Plantaginaceae and Rosaceae gametophytic SI is controlled by the pistil-specific S-RNases and the pollen S-locus F-box proteins but non-S-specific factors, namely modifiers, are ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx336
更新日期:2017-11-02 00:00:00
abstract::Charles Darwin made extensive observations of the pollination biology of a wide variety of plants. He carefully documented the consequences of self-pollination and described species that were self-sterile but that could easily be crossed with other plants of the same species. He believed that compatibility was control...
journal_title:Journal of experimental botany
pub_type: 历史文章,杂志文章,评审
doi:10.1093/jxb/erp024
更新日期:2009-01-01 00:00:00
abstract::The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which has both constitutiv...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru528
更新日期:2015-04-01 00:00:00
abstract::Although the mechanisms of nodule N(2) fixation in legumes are now well documented, some uncertainty remains on the metabolic consequences of water deficit. In most cases, little consideration is given to other organs and, therefore, the coordinated changes in metabolism in leaves, roots, and nodules are not well know...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ers367
更新日期:2013-02-01 00:00:00
abstract::Nitric oxide has been reported to act as a signalling molecule in different plant tissues and to participate in a variety of physiological processes. It is produced by different enzymes and sources. The root-specific plasma membrane-bound enzymes forming NO from the substrates nitrate and nitrite are of particular int...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj058
更新日期:2006-01-01 00:00:00
abstract::Rates of photosynthesis can be lower in plants grown under conditions of high leaf-to-air vapour pressure difference (VPD) than under low VPD. Leaf phenotype plasticity is a primary factor determining photosynthetic responses to environmental stimuli. However, it remains unclear how changes in leaf anatomical traits d...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz238
更新日期:2019-09-24 00:00:00
abstract::The BES1/BZR1 transcription factors regulate the expression of brassinosteroid responsive genes and play pivotal roles in plant development. However, the function of BES1/BZR1 regulating kernel development remains unclear. In this study, maize ZmBES1/BZR1-5 is found to positively regulate kernel size. Candidate-gene a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa544
更新日期:2020-11-18 00:00:00
abstract::Research on the functional properties of nucleosome structure and composition dynamics has revealed that chromatin-level regulation is an essential component of light signalling and clock function in plants, two processes that rely extensively on transcriptional controls. In particular, several types of histone post-t...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/eru011
更新日期:2014-06-01 00:00:00
abstract::Emergent aquatic macrophytes growing in waterlogged anaerobic sediments overlain by deep water require particularly efficient ventilating systems. In Phragmites australis (Cav.) Trin. ex Steud, pressurized gas flows, generated by humidity-induced diffusion of air into leaf sheaths, enhance oxygen transport to below-gr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm017
更新日期:2007-01-01 00:00:00
abstract::In the Arabidopsis mutant sdd1-1, a point mutation in a single gene (SDD1) causes specific alterations in stomatal density and distribution. In comparison to the wild type (C24), abaxial surfaces of sdd1-1 rosette leaves have about 2.5-fold higher stomatal densities. This mutant was used to study the consequence of st...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg087
更新日期:2003-02-01 00:00:00
abstract::The production of bioenergy from grasses has been developing quickly during the last decade, with Miscanthus being among the most important choices for production of bioethanol. However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure. Cell walls are thought to disp...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv183
更新日期:2015-07-01 00:00:00
abstract::Crop water use efficiency (WUE) has come into sharp focus as population growth and climate change place increasing strain on the water used in cropping. Rainfed crops are being challenged by an upward trend in evaporative demand as average temperatures rise and, in many regions, there is an increased irregularity and ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa002
更新日期:2020-04-06 00:00:00
abstract::A major QTL affecting root traits and leaf ABA concentration was identified in maize (Zea mays L.) and named root-ABA1. For this QTL, back-cross-derived lines (BDLs) homozygous either for the (+) or for the (-) allele increasing or decreasing, respectively, root size and leaf ABA concentration, were developed. This st...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl161
更新日期:2007-01-01 00:00:00
abstract::Cytoplasmic male sterility (CMS) results from incompatibility between nuclear and cytoplasmic genomes, and is characterized by the inability to produce viable pollen. The restoration of male fertility generally involves the introgression of nuclear genes, termed restorers of fertility (Rf). CMS has been widely used fo...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru388
更新日期:2014-12-01 00:00:00
abstract::The aim of this work was to investigate the importance of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) in potato carbohydrate metabolism. For this purpose, the cytosolic isoform of phosphorylating GAPC was cloned and used for an antisense approach to generate transgenic potato plants that ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj207
更新日期:2006-01-01 00:00:00
abstract::The fact that silicon application alleviates water deficit stress has been widely reported, but the underlying mechanism remains unclear. Here the effects of silicon on water uptake and transport of sorghum seedlings (Sorghum bicolor L.) growing under polyethylene glycol-simulated osmotic stress in hydroponic culture ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru220
更新日期:2014-09-01 00:00:00
abstract::Small non-coding RNAs are key post-transcriptional and transcriptional regulators of plant gene expression in angiosperm sporophytes. In recent years, gametophytic small RNAs have also been investigated, predominantly in Arabidopsis male gametophytes, revealing features in common with the sporophyte as well as some su...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erq399
更新日期:2011-03-01 00:00:00