Abstract:
:The BES1/BZR1 transcription factors regulate the expression of brassinosteroid responsive genes and play pivotal roles in plant development. However, the function of BES1/BZR1 regulating kernel development remains unclear. In this study, maize ZmBES1/BZR1-5 is found to positively regulate kernel size. Candidate-gene association analysis showed that four and three SNPs related to ZmBES1/BZR1-5 were significantly associated with kernel width and 100-kernel weight in 513 diverse maize inbred lines, respectively. Overexpression of ZmBES1/BZR1-5 gene in Arabidopsis and rice both significantly increased seed size and weight, as well as smaller kernel produced in maize Mu transposon insertion and EMS mutants. The ZmBES1/BZR1-5 protein contains bHLH and BAM domains, shows no transcriptional activity as monomer but forms homodimer through BAM domain, and locates in nucleus. Chromatin immunoprecipitation sequencing (ChIP-seq), yeast one-hybrid (Y1H) and dual-luciferase assay demonstrate that ZmBES1/BZR1-5 protein binds to the promoter of AP2/EREBP genes (Zm00001d010676 and Zm00001d032077) and inhibits their transcription. cDNA library screening shows that ZmBES1/BZR1-5 interacts with casein kinase II subunit β4 (ZmCKIIβ4) and ferredoxin 2 (ZmFdx2) in vitro and vivo, respectively. Taken together, the study suggests that ZmBES1/BZR1-5 positively regulates kernel size and provides new insights into understanding the mechanism of kernel development in maize.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Sun F,Ding L,Feng W,Cao Y,Lu F,Yang Q,Li W,Lu Y,Shabek N,Fu F,Yu Hdoi
10.1093/jxb/eraa544subject
Has Abstractpub_date
2020-11-18 00:00:00eissn
0022-0957issn
1460-2431pii
5988956pub_type
杂志文章abstract::Hydration at low temperatures, commonly referred to as cold stratification, is widely used for releasing dormancy and triggering germination in a wide range of species including wheat. However, the molecular mechanism that underlies its effect on germination has largely remained unknown. Our previous studies showed th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw172
更新日期:2016-05-01 00:00:00
abstract::Photo-oxidative stress was imposed under natural solar radiation on exposed and shaded sections of detached fruit of immature green tomato (Lycopersicon esculentum Miller = Solanum lycopersicum L.) mutants (anthocyanin absent, beta-carotene, Delta, and high pigment-1) and their nearly isogenic parents ('Ailsa Craig' a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-01-23 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru185
更新日期:2014-07-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa425
更新日期:2020-09-11 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err047
更新日期:2011-06-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh227
更新日期:2004-09-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.360.1587
更新日期:2001-07-01 00:00:00
abstract::Photosynthesis has become a major trait of interest for cereal yield improvement as breeders appear to have reached the theoretical genetic limit for harvest index, the mass of grain as a proportion of crop biomass. Yield improvements afforded by the adoption of green revolution dwarfing genes to wheat and rice are be...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa077
更新日期:2020-04-06 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh234
更新日期:2004-10-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm037
更新日期:2007-01-01 00:00:00
abstract::In a non-model staple crop like wheat (Triticum aestivumI L.), functional validation of potential drought stress responsive genes identified in Arabidopsis could provide gene targets for breeding. Virus-induced gene silencing (VIGS) of genes of interest can overcome the inherent problems of polyploidy and limited tran...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert003
更新日期:2013-03-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq040
更新日期:2010-06-01 00:00:00
abstract::In peach (Prunus persica L. Batsch.) the degradation of the pectic compounds of the cell wall is considered to be the principal component responsible for fruit softening. Many genes encoding enzymes acting on the different polymers of the pectic matrix have been shown to be highly expressed during the late phases of s...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj043
更新日期:2006-01-01 00:00:00
abstract::All members of the sulphotransferase (SOT, EC 2.8.2.-) protein family use 3'-phosphoadenosine 5'-phosphosulphate (PAPS) as the sulphuryl donor and transfer the sulphonate group to an appropriate hydroxyl group of several classes of substrates. These enzymes have highly conserved domains and can be found in eubacteria ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erh183
更新日期:2004-08-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw100
更新日期:2016-06-01 00:00:00
abstract::A cDNA clone was selected as a candidate for the catalytic subunit of phospho-pyruvate dehydrogenase phosphatase (PDP) by screening a Zea mays expressed sequence tag database with the bovine PDP deduced amino acid sequence. Both strands of the cDNA were completely sequenced. The maize clone contains an open reading fr...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Site-directed mutations have been generated in the pore and voltage sensor regions of the inwardly rectifying potassium channels KAT1 and KST1. The properties of mutant channels have been analysed in Xenopus oocytes, and give insights into the structure-function relations of these channels. ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/48.Special_Issue.415
更新日期:1997-03-01 00:00:00
abstract::In order to understand the role of cytosolic antioxidant enzymes in drought stress protection, transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants overexpressing cytosolic Cu/Zn-superoxide dismutase (cytsod) (EC 1.15.1.1) or ascorbate peroxidase (cytapx) (EC 1.11.1.1) alone, or in combination, were produced and t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq432
更新日期:2011-05-01 00:00:00
abstract::Cell wall disassembly in softening fruits is a complex process involving the cumulative action of many families of wall-modifying proteins on interconnected polysaccharide matrices. One strategy to elucidate the in vivo substrates of specific enzymes and their relative importance and contribution to wall modification ...
journal_title:Journal of experimental botany
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/ery459
更新日期:2019-04-15 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erh179
更新日期:2004-08-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw188
更新日期:2016-06-01 00:00:00
abstract::Flowering is a crucial developmental stage in the plant life cycle. A number of different factors, from environmental to chemical, can trigger flowering. In pineapple, and other bromeliads, it has been proposed that flowering is triggered by a small burst of ethylene production in the meristem in response to environme...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl167
更新日期:2006-01-01 00:00:00
abstract::Protein tyrosine nitration is a post-translational modification mediated by reactive nitrogen species (RNS) that is associated with nitro-oxidative damage. No information about this process is available in relation to higher plants during development and senescence. Using pea plants at different developmental stages (...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert006
更新日期:2013-02-01 00:00:00
abstract::Phytocystatins are inhibitors of cysteine proteinases from plants putatively involved in defence and as endogenous regulators of protein turnover. Seven genes encoding cystatins (HvCPI-1 to HvCPI-7), identified from EST collections and from an endosperm cDNA library, have been characterized. The intron-exon structure ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl200
更新日期:2006-01-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa477
更新日期:2020-10-17 00:00:00
abstract::Changes in root hydraulic resistance in response to alterations in nitrate supply were explored in detail as a potential mechanism that allows plants to respond rapidly to changes in their environment. Sunflower (Helianthus annuus cv. Holiday) plants grown hydroponically with limited nitrate availability (200 micromol...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm118
更新日期:2007-01-01 00:00:00