Abstract:
:Non-invasive X-ray computed tomography (XRCT) is increasingly used in rhizosphere research to visualize development of soil-root interfaces in situ. However, exposing living systems to X-rays can potentially impact their processes and metabolites. In order to evaluate these effects, we assessed the responses of rhizosphere processes 1 and 24 h after a low X-ray exposure (0.81 Gy). Changes in root gene expression patterns occurred 1 h after exposure with down-regulation of cell wall-, lipid metabolism-, and cell stress-related genes, but no differences remained after 24 h. At either time point, XRCT did not affect either root antioxidative enzyme activities or the composition of the rhizosphere bacterial microbiome and microbial growth parameters. The potential activities of leucine aminopeptidase and phosphomonoesterase were lower at 1 h, but did not differ from the control 24 h after exposure. A time delay of 24 h after a low X-ray exposure (0.81 Gy) was sufficient to reverse any effects on the observed rhizosphere systems. Our data suggest that before implementing novel experimental designs involving XRCT, a study on its impact on the investigated processes should be conducted.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Ganther M,Yim B,Ibrahim Z,Bienert MD,Lippold E,Maccario L,Sørensen SJ,Bienert GP,Vetterlein D,Heintz-Buschart A,Blagodatskaya E,Smalla K,Tarkka MTdoi
10.1093/jxb/eraa262subject
Has Abstractpub_date
2020-09-19 00:00:00pages
5603-5614issue
18eissn
0022-0957issn
1460-2431pii
5848217journal_volume
71pub_type
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