Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots.


:The effect of heavy metals on the modification of plasma membrane H(+)-ATPase (EC activity in cucumber roots was studied. In plants stressed for 2 h with 10 microM or 100 microM Cd, Cu or Ni the hydrolytic as well as the transporting activity of H(+)-ATPase in the plasma membranes of root cells was decreased. Transcript levels of Cucumis sativus plasma membrane H(+)-ATPase in roots treated with 10 microM Cd, Cu, or Ni as well as with 100 microM Cu or Ni were similar to the control, indicating that the action of metals did not involve the gene expression level. Only in roots exposed to 100 microM Cd was the level of plasma membrane H(+)-ATPase mRNA markedly decreased. The inhibition of the plasma membrane proton pump caused by 100 microM Cd, Cu and Ni was partially diminished in the presence of cantharidin, a specific inhibitor of protein phosphatases. Western blot analysis with the antibody against phosphothreonine confirmed that decreased activity of plasma membrane H(+)-ATPase under heavy metals resulted from dephosphorylation of the enzyme protein. Taken together, these data strongly indicated that alteration of the enzyme under heavy metal stresses was mainly due to the post-translational modification of its proteins in short-term experiments.


J Exp Bot


Janicka-Russak M,Kabała K,Burzyński M,Kłobus G




Has Abstract


2008-01-01 00:00:00














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    abstract::Sugars regulate the expression of many genes at the transcriptional level. In Arabidopsis thaliana, sugars induce or repress the expression of >1800 genes, including the STP1 (SUGAR TRANSPORTER PROTEIN 1) gene, which encodes an H(+)/monosaccharide cotransporter. STP1 transcript levels decrease more rapidly after the a...

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    authors: Cordoba E,Aceves-Zamudio DL,Hernández-Bernal AF,Ramos-Vega M,León P

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  • PhOBF1, a petunia ocs element binding factor, plays an important role in antiviral RNA silencing.

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    journal_title:Journal of experimental botany

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    authors: Sun D,Li S,Niu L,Reid MS,Zhang Y,Jiang CZ

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  • Small-peptide signals that control root nodule number, development, and symbiosis.

    abstract::Many legumes have the capacity to enter into a symbiotic association with soil bacteria generically called 'rhizobia' that results in the formation of new lateral organs on roots called nodules within which the rhizobia fix atmospheric nitrogen (N). Up to 200 million tonnes of N per annum is fixed by this association....

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    pub_type: 杂志文章,评审


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  • The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1.

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  • An evolutionary population structure model reveals pleiotropic effects of GmPDAT for traits related to seed size and oil content in soybean.

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    authors: Ouyang W,Struik PC,Yin X,Yang J

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    authors: Besnard G,Christin PA,Malé PJ,Lhuillier E,Lauzeral C,Coissac E,Vorontsova MS

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    authors: Pajoro A,Biewers S,Dougali E,Leal Valentim F,Mendes MA,Porri A,Coupland G,Van de Peer Y,van Dijk AD,Colombo L,Davies B,Angenent GC

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    authors: Valeska Zeisler-Diehl V,Migdal B,Schreiber L

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    authors: Sun L,Tian J,Zhang H,Liao H

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    pub_type: 杂志文章


    authors: Hiwasa K,Kinugasa Y,Amano S,Hashimoto A,Nakano R,Inaba A,Kubo Y

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    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Misyura M,Colasanti J,Rothstein SJ

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    authors: Liu R,Cao SK,Sayyed A,Yang HH,Zhao J,Wang X,Jia RX,Sun F,Tan BC

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  • Different ethylene receptors show an increased expression during the ripening of strawberries: does such an increment imply a role for ethylene in the ripening of these non-climacteric fruits?

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    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Trainotti L,Pavanello A,Casadoro G

    更新日期:2005-08-01 00:00:00

  • Metabolic analysis of kiwifruit (Actinidia deliciosa) berries from extreme genotypes reveals hallmarks for fruit starch metabolism.

    abstract::Tomato, melon, grape, peach, and strawberry primarily accumulate soluble sugars during fruit development. In contrast, kiwifruit (Actinidia Lindl. spp.) and banana store a large amount of starch that is released as soluble sugars only after the fruit has reached maturity. By integrating metabolites measured by gas chr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Nardozza S,Boldingh HL,Osorio S,Höhne M,Wohlers M,Gleave AP,MacRae EA,Richardson AC,Atkinson RG,Sulpice R,Fernie AR,Clearwater MJ

    更新日期:2013-11-01 00:00:00

  • Functional relationship between mTERF4 and GUN1 in retrograde signaling.

    abstract::Plastid-to-nucleus retrograde signaling plays an important role in regulating the expression of photosynthesis-associated nuclear genes (PhANGs) in accordance with physiological demands on chloroplast biogenesis and function. Despite its fundamental importance, little is known about the molecular nature of the plastid...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sun X,Xu D,Liu Z,Kleine T,Leister D

    更新日期:2016-06-01 00:00:00

  • Life and death under salt stress: same players, different timing?

    abstract::Salinity does not only stress plants but also challenges human life and the economy by posing severe constraints upon agriculture. To understand salt adaptation strategies of plants, it is central to extend agricultural production to salt-affected soils. Despite high impact and intensive research, it has been difficul...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Ismail A,Takeda S,Nick P

    更新日期:2014-07-01 00:00:00