Characterization and functional investigation of an Arabidopsis cDNA encoding a homologue to the d-PGMase superfamily.

Abstract:

:An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase superfamily: cofactor-dependent phosphoglycerate mutases (d-PGM-ases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Preliminary phylogenetic studies indicated that At-74 cDNA and its close homologue in Arabidopsis, At-74H, belong, however, to an equally distinct group. At-74 was ubiquitously expressed in vegetative organs and induced by glucose. The At-74 cDNA was overexpressed in A. thaliana to investigate its function, but this overexpression did not result in a clear phenotype. Enzymatic assays performed on At-74-overproducing transgenic plants or E. coli cells showed no increase in either the activities of cofactor-dependent and -independent phosphoglycerate mutases (i-PGMases) and F2,6Pase or that of acid phosphatases. The possible role of At-74 in plant metabolism was further investigated by carbon partitioning experiments with [U-(14)C] glucose and measurements of soluble sugars in both young leaves and roots. Two overexpressing At-74 lines showed a clear increase in glucose uptake. This paper introduces the At-74 homologue of the d-PGMase superfamily members and supports a possible role of At-74 in carbohydrate metabolism.

journal_name

J Exp Bot

authors

Bourgis F,Botha FC,Mani S,Hiten FN,Rigden DJ,Verbruggen N

doi

10.1093/jxb/eri105

keywords:

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

1129-42

issue

414

eissn

0022-0957

issn

1460-2431

pii

eri105

journal_volume

56

pub_type

杂志文章
  • The inverse relationship between solar-induced fluorescence yield and photosynthetic capacity: benefits for field phenotyping.

    abstract::Improving photosynthesis is considered a promising way to increase crop yield to feed a growing population. Realizing this goal requires non-destructive techniques to quantify photosynthetic variation among crop cultivars. Despite existing remote sensing-based approaches, it remains a question whether solar-induced fl...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa537

    authors: Fu P,Meacham-Hensold K,Siebers MH,Bernacchi CJ

    更新日期:2020-12-19 00:00:00

  • Phytohormone regulation of root growth triggered by P deficiency or Al toxicity.

    abstract::Phosphorus (P) deficiency and aluminum (Al) toxicity often coexist and limit plant growth on acid soils. It has been well documented that both P deficiency and Al toxicity alter root growth, including inhibition of primary roots and promotion of lateral roots. This suggests that plants adapt to both stresses through a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erw188

    authors: Sun L,Tian J,Zhang H,Liao H

    更新日期:2016-06-01 00:00:00

  • Honeydew-associated microbes elicit defense responses against brown planthopper in rice.

    abstract::Feeding of sucking insects, such as the rice brown planthopper (Nilaparvata lugens; BPH), causes only limited mechanical damage on plants that is otherwise essential for injury-triggered defense responses against herbivores. In pursuit of complementary BPH elicitors perceived by plants, we examined the potential effec...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz041

    authors: Wari D,Kabir MA,Mujiono K,Hojo Y,Shinya T,Tani A,Nakatani H,Galis I

    更新日期:2019-03-11 00:00:00

  • 1-Aminocyclopropane-1-carboxylic acid and abscisic acid during the germination of sugar beet (Beta vulgaris L.): a comparative study of fruits and seeds.

    abstract::The control of sugar beet (Beta vulgaris L.) germination by plant hormones was studied by comparing fruits and seeds. Treatment of sugar beet fruits and seeds with gibberellins, brassinosteroids, auxins, cytokinins, and jasmonates or corresponding hormone biosynthesis inhibitors did not appreciably affect radicle emer...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm162

    authors: Hermann K,Meinhard J,Dobrev P,Linkies A,Pesek B,Hess B,Machácková I,Fischer U,Leubner-Metzger G

    更新日期:2007-01-01 00:00:00

  • Exploiting heterogeneous environments: does photosynthetic acclimation optimize carbon gain in fluctuating light?

    abstract::Plants have evolved complex mechanisms to balance the efficient use of absorbed light energy in photosynthesis with the capacity to use that energy in assimilation, so avoiding potential damage from excess light. This is particularly important under natural light, which can vary according to weather, solar movement an...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv055

    authors: Retkute R,Smith-Unna SE,Smith RW,Burgess AJ,Jensen OE,Johnson GN,Preston SP,Murchie EH

    更新日期:2015-05-01 00:00:00

  • Functional evolution of C(4) pyruvate, orthophosphate dikinase.

    abstract::Pyruvate,orthophosphate dikinase (PPDK) plays a controlling role in the PEP-regeneration phase of the C(4) photosynthetic pathway. Earlier studies have fully documented its biochemical properties and its post-translational regulation by the PPDK regulatory protein (PDRP). However, the question of its evolution into th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err058

    authors: Chastain CJ,Failing CJ,Manandhar L,Zimmerman MA,Lakner MM,Nguyen TH

    更新日期:2011-05-01 00:00:00

  • Style-by-style analysis of two sporadic self-compatible Solanum chacoense lines supports a primary role for S-RNases in determining pollen rejection thresholds.

    abstract::A method for the quantification of S-RNase levels in single styles of self-incompatible Solanum chacoense was developed and applied toward an experimental determination of the S-RNase threshold required for pollen rejection. It was found that, when single style values are averaged, accumulated levels of the S(11)- and...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj147

    authors: Qin X,Liu B,Soulard J,Morse D,Cappadocia M

    更新日期:2006-01-01 00:00:00

  • A method for the isolation of root hairs from the model legume Medicago truncatula.

    abstract::A new method for the isolation of root hairs from the model legume, Medicago truncatula, was developed. The procedure involves the propagation of detached roots on agar plates and the collection of root hairs by immersion in liquid nitrogen. Yields of up to 40 micro g of root hair protein were obtained from 50-100 roo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg245

    authors: Ramos J,Bisseling T

    更新日期:2003-10-01 00:00:00

  • Light and CO2 do not affect the mesophyll conductance to CO2 diffusion in wheat leaves.

    abstract::In C(3) plants, diffusion of CO(2) into leaves is restricted by stomata and subsequently by the intercellular airspaces and liquid phase into chloroplasts. While considerable information exists on the effect of environmental conditions on stomatal conductance (g(s)), little is known on whether the mesophyll conductanc...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp035

    authors: Tazoe Y,von Caemmerer S,Badger MR,Evans JR

    更新日期:2009-01-01 00:00:00

  • On the move: induced resistance in monocots.

    abstract::Although plants possess an arsenal of constitutive defences such as structural barriers and preformed antimicrobial defences, many attackers are able to overcome the pre-existing defence layers. In response, a range of inducible plant defences is set up to battle these pathogens. These mechanisms, commonly integrated ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/ers248

    authors: Balmer D,Planchamp C,Mauch-Mani B

    更新日期:2013-03-01 00:00:00

  • In situ and in vitro senescence induced by KCl stress: nutritional imbalance, lipid peroxidation and antioxidant metabolism.

    abstract::Sunflower (Helianthus annuus L. cv. SH222) plants and calli were exposed to KCl stress for three weeks. Calli were more tolerant to KCl than plants. KCl stress decreased NO(-)(3), Mn, Fe and B levels in whole plants and P, Ca and Mg in shoots. NO(-)(3), P, Ca, Mg, Mn, and B levels decreased in 100 mM-stressed calli. C...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Santos CL,Campos A,Azevedo H,Caldeira G

    更新日期:2001-02-01 00:00:00

  • Involvement of reactive oxygen species in endosperm cap weakening and embryo elongation growth during lettuce seed germination.

    abstract::Endosperm cap (CAP) weakening and embryo elongation growth are prerequisites for the completion of lettuce seed germination. Although it has been proposed that the cell wall loosening underlying these processes results from an enzymatic mechanism, it is still unclear which enzymes are involved. Here it is shown that r...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru167

    authors: Zhang Y,Chen B,Xu Z,Shi Z,Chen S,Huang X,Chen J,Wang X

    更新日期:2014-07-01 00:00:00

  • Deficit irrigation for reducing agricultural water use.

    abstract::At present and more so in the future, irrigated agriculture will take place under water scarcity. Insufficient water supply for irrigation will be the norm rather than the exception, and irrigation management will shift from emphasizing production per unit area towards maximizing the production per unit of water consu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl165

    authors: Fereres E,Soriano MA

    更新日期:2007-01-01 00:00:00

  • Volatile fingerprints of seeds of four species indicate the involvement of alcoholic fermentation, lipid peroxidation, and Maillard reactions in seed deterioration during ageing and desiccation stress.

    abstract::The volatile compounds released by orthodox (desiccation-tolerant) seeds during ageing can be analysed using gas chromatography-mass spectrometry (GC-MS). Comparison of three legume species (Pisum sativum, Lathyrus pratensis, and Cytisus scoparius) during artificial ageing at 60% relative humidity and 50 °C revealed v...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers307

    authors: Colville L,Bradley EL,Lloyd AS,Pritchard HW,Castle L,Kranner I

    更新日期:2012-11-01 00:00:00

  • Contrasting adaptive strategies to terminal drought-stress gradients in Mediterranean legumes: phenology, productivity, and water relations in wild and domesticated Lupinus luteus L.

    abstract::Our understanding of within-species annual plant adaptation to rainfall gradients is fragmented. Broad-scale ecological applications of Grime's C-S-R triangle are often superficial, while detailed drought physiology tends to be narrow, focusing on elite cultivars. The former lack the detail to explain how plants respo...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru006

    authors: Berger JD,Ludwig C

    更新日期:2014-11-01 00:00:00

  • The strawberry transcription factor FaMYB1 inhibits the biosynthesis of proanthocyanidins in Lotus corniculatus leaves.

    abstract::Proanthocyanidins (PAs) are agronomically important biopolymers in higher plants composed primarily of catechin and epicatechin units. The biosynthesis of these natural products is regulated by transcription factors including proteins of the R2R3MYB class. To gain insight into the genetic control of the catechin and e...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erq344

    authors: Paolocci F,Robbins MP,Passeri V,Hauck B,Morris P,Rubini A,Arcioni S,Damiani F

    更新日期:2011-01-01 00:00:00

  • Tight regulation of expression of two Arabidopsis cytosolic Hsp90 genes during embryo development.

    abstract::The spatial and temporal distribution of expression of two cytosolic members of the AtHsp90 gene family was assessed during early development. In stressed transgenic plants bearing the AtHsp90-3 promoter, beta-glucuronidase (GUS) activity was strong in meristematic tissues. Expression was also detected in vascular tis...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri035

    authors: Prasinos C,Krampis K,Samakovli D,Hatzopoulos P

    更新日期:2005-02-01 00:00:00

  • The roles of pleiotropy and close linkage as revealed by association mapping of yield and correlated traits of wheat (Triticum aestivum L.).

    abstract::Grain yield (GY) of bread wheat (Triticum aestivum L.) is quantitatively inherited. Correlated GY-syndrome traits such as plant height (PH), heading date (HD), thousand grain weight (TGW), test weight (TW), grains per ear (GPE), and ear weight (EW) influence GY. Most quantitative genetics studies assessed the multiple...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx214

    authors: Schulthess AW,Reif JC,Ling J,Plieske J,Kollers S,Ebmeyer E,Korzun V,Argillier O,Stiewe G,Ganal MW,Röder MS,Jiang Y

    更新日期:2017-07-10 00:00:00

  • Molecular analysis and control of cysteine biosynthesis: integration of nitrogen and sulphur metabolism.

    abstract::Since cysteine is the first committed molecule in plant metabolism containing both sulphur and nitrogen, the regulation of its biosynthesis is critically important. Cysteine itself is required for the production of an abundance of key metabolites in diverse pathways. Plants alter their metabolism to compensate for sul...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erh136

    authors: Hesse H,Nikiforova V,Gakière B,Hoefgen R

    更新日期:2004-06-01 00:00:00

  • The NPR1-dependent salicylic acid signalling pathway is pivotal for enhanced salt and oxidative stress tolerance in Arabidopsis.

    abstract::The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which has both constitutiv...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru528

    authors: Jayakannan M,Bose J,Babourina O,Shabala S,Massart A,Poschenrieder C,Rengel Z

    更新日期:2015-04-01 00:00:00

  • Ion-specific mechanisms of osmoregulation in bean mesophyll cells.

    abstract::Transient kinetics of net H+, K+, Ca2+, and Cl- fluxes were measured non-invasively, using an ion-selective microelectrode technique, for bean (Vicia faba L.) leaf mesophyll in response to 150 mM mannitol treatment. In a parallel set of experiments, changes in the plasma membrane potential and the total proline conten...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Shabala S,Babourina O,Newman I

    更新日期:2000-07-01 00:00:00

  • Hydraulic conductivity and PAT determine hierarchical resource partitioning and ramet development along Fragaria stolons.

    abstract::Co-ordination of metabolic and physiological activity between plant parts is key to the control of growth and development. Here the movement of resources and their allocation between mother plants and daughter ramets along Fragaria stolons was quantified with respect to hierarchy. Gradients of internodal ramet leaf wa...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers155

    authors: Atkinson CJ,Else MA

    更新日期:2012-09-01 00:00:00

  • Use of transcriptomics and co-expression networks to analyze the interconnections between nitrogen assimilation and photorespiratory metabolism.

    abstract::Nitrogen is one of the most important nutrients for plants and, in natural soils, its availability is often a major limiting factor for plant growth. Here we examine the effect of different forms of nitrogen nutrition and of photorespiration on gene expression in the model legume Lotus japonicus with the aim of identi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw170

    authors: Pérez-Delgado CM,Moyano TC,García-Calderón M,Canales J,Gutiérrez RA,Márquez AJ,Betti M

    更新日期:2016-05-01 00:00:00

  • Effect of P-deficiency on photoassimilate partitioning and rhythmic changes in fruit and stem diameter of tomato (Lycopersicon esculentum) during fruit growth.

    abstract::Tomato (Lycopersicon esculentum) plants were grown in liquid culture inside the greenhouse of Hiroshima University, Japan. At the first fruiting stage, P was withdrawn from the rooting medium for a period of 19 d and its effect was studied on photosynthesis, stomatal conductance, transpiration, partitioning of 13C and...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg273

    authors: Fujita K,Okada M,Lei K,Ito J,Ohkura K,Adu-Gyamfi JJ,Mohapatra PK

    更新日期:2003-11-01 00:00:00

  • A copia-like retrotransposon insertion in the upstream region of the SHATTERPROOF1 gene, BnSHP1.A9, is associated with quantitative variation in pod shattering resistance in oilseed rape.

    abstract::Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here, we show that the pod shatter resistance at quantitative trait locus qSRI.A9.1 is controlled by one of the B...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa281

    authors: Liu J,Zhou R,Wang W,Wang H,Qiu Y,Raman R,Mei D,Raman H,Hu Q

    更新日期:2020-09-19 00:00:00

  • Melatonin promotes ripening and improves quality of tomato fruit during postharvest life.

    abstract::In this study, the effect of melatonin on the postharvest ripening and quality improvement of tomato fruit was carried out. The tomatoes were immersed in exogenous melatonin for 2h, and then the related physiological indicators and the expression of genes during post-harvest life were evaluated. Compared with control ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru332

    authors: Sun Q,Zhang N,Wang J,Zhang H,Li D,Shi J,Li R,Weeda S,Zhao B,Ren S,Guo YD

    更新日期:2015-02-01 00:00:00

  • Interactive effects of water stress and xylem-limited bacterial infection on the water relations of a host vine.

    abstract::Xylella fastidiosa, a xylem-limited bacterial pathogen that causes bacterial leaf scorch in its hosts, has a diverse and extensive host range among plant species worldwide. Previous work has shown that water stress enhances leaf scorch symptom severity and progression along the stem in Parthenocissus quinquefolia infe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg046

    authors: McElrone AJ,Sherald JL,Forseth IN

    更新日期:2003-01-01 00:00:00

  • Selective transcriptional down-regulation of anther invertases precedes the failure of pollen development in water-stressed wheat.

    abstract::Water deficit during male meiosis in wheat (Triticum aestivum L.) causes pollen sterility. With a view to identifying the internal trigger for this failure, it was found that water stress specifically impairs the activities of vacuolar and cell-wall invertases in anthers prior to the arrest of pollen development. The ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri018

    authors: Koonjul PK,Minhas JS,Nunes C,Sheoran IS,Saini HS

    更新日期:2005-01-01 00:00:00

  • The family of Peps and their precursors in Arabidopsis: differential expression and localization but similar induction of pattern-triggered immune responses.

    abstract::In Arabidopsis thaliana, the endogenous danger peptides, AtPeps, have been associated with plant defences reminiscent of those induced in pattern-triggered immunity. AtPeps are perceived by two homologous receptor kinases, PEPR1 and PEPR2, and are encoded in the C termini of the PROPEP precursors. Here, we report that...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert330

    authors: Bartels S,Lori M,Mbengue M,van Verk M,Klauser D,Hander T,Böni R,Robatzek S,Boller T

    更新日期:2013-12-01 00:00:00

  • Elevated CO2 has concurrent effects on leaf and grain metabolism but minimal effects on yield in wheat.

    abstract::While the general effect of CO2 enrichment on photosynthesis, stomatal conductance, N content, and yield has been documented, there is still some uncertainty as to whether there are interactive effects between CO2 enrichment and other factors, such as temperature, geographical location, water availability, and cultiva...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa330

    authors: Tcherkez G,Ben Mariem S,Larraya L,García-Mina JM,Zamarreño AM,Paradela A,Cui J,Badeck FW,Meza D,Rizza F,Bunce J,Han X,Tausz-Posch S,Cattivelli L,Fangmeier A,Aranjuelo I

    更新日期:2020-10-07 00:00:00