Iron uptake system mediates nitrate-facilitated cadmium accumulation in tomato (Solanum lycopersicum) plants.

Abstract:

:Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered hydroponic culture to investigate the direct effect of N-form on Cd uptake. Wild-type plants fed NO₃⁻ accumulated more Cd than plants fed NH₄⁺. Iron uptake and LeIRT1 expression in roots were also greater in plants fed NO₃⁻. However, in mutant T3238fer which loses FER function, LeIRT1 expression in roots was almost completely terminated, and the difference between NO₃⁻ and NH₄⁺ treatments vanished. As a result, the N-form had no effect on Cd uptake in this mutant. Furthermore, suppression of LeIRT1 expression by NO synthesis inhibition with either tungstate or L-NAME, also substantially inhibited Cd uptake in roots, and the difference between N-form treatments was diminished. Considering all of these findings, it was concluded that the up-regulation of the Fe uptake system was responsible for NO₃⁻-facilitated Cd accumulation in plants.

journal_name

J Exp Bot

authors

Luo BF,Du ST,Lu KX,Liu WJ,Lin XY,Jin CW

doi

10.1093/jxb/ers036

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

3127-36

issue

8

eissn

0022-0957

issn

1460-2431

pii

ers036

journal_volume

63

pub_type

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