Overexpression of mitochondrial uncoupling protein 1 (UCP1) induces a hypoxic response in Nicotiana tabacum leaves.

Abstract:

:Mitochondrial uncoupling protein 1 (UCP1) decreases reactive oxygen species production under stress conditions by uncoupling the electrochemical gradient from ATP synthesis. This study combined transcriptome profiling with experimentally induced hypoxia to mechanistically dissect the impact of Arabidopsis thaliana UCP1 (AtUCP1) overexpression in tobacco. Transcriptomic analysis of AtUCP1-overexpressing (P07) and wild-type (WT) plants was carried out using RNA sequencing. Metabolite and carbohydrate profiling of hypoxia-treated plants was performed using (1)H-nuclear magnetic resonance spectroscopy and high-performance anion-exchange chromatography with pulsed amperometric detection. The transcriptome of P07 plants revealed a broad induction of stress-responsive genes that were not strictly related to the mitochondrial antioxidant machinery, suggesting that overexpression of AtUCP1 imposes a strong stress response within the cell. In addition, transcripts that mapped into carbon fixation and energy expenditure pathways were broadly altered. It was found that metabolite markers of hypoxic adaptation, such as alanine and tricarboxylic acid intermediates, accumulated in P07 plants under control conditions at similar rates to WT plants under hypoxia. These findings indicate that constitutive overexpression of AtUCP1 induces a hypoxic response. The metabolites that accumulated in P07 plants are believed to be important in signalling for an improvement in carbon assimilation and induction of a hypoxic response. Under these conditions, mitochondrial ATP production is less necessary and fermentative glycolysis becomes critical to meet cell energy demands. In this scenario, the more flexible energy metabolism along with an intrinsically activated hypoxic response make these plants better adapted to face several biotic and abiotic stresses.

journal_name

J Exp Bot

authors

Barreto P,Okura V,Pena IA,Maia R,Maia IG,Arruda P

doi

10.1093/jxb/erv460

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

301-13

issue

1

eissn

0022-0957

issn

1460-2431

pii

erv460

journal_volume

67

pub_type

杂志文章
  • Plants grow with a little help from their organelle friends.

    abstract::Chloroplasts and mitochondria are indispensable for plant development. They not only provide energy and carbon sources to cells, but also have evolved to become major players in a variety of processes such as amino acid metabolism, hormone biosynthesis and cellular signalling. As semi-autonomous organelles, they conta...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erw399

    authors: Van Dingenen J,Blomme J,Gonzalez N,Inzé D

    更新日期:2016-12-01 00:00:00

  • Phenotyping transgenic wheat for drought resistance.

    abstract::Realistic experimental protocols to screen for drought adaptation in controlled conditions are crucial if high throughput phenotyping is to be used for the identification of high performance lines, and is especially important in the evaluation of transgenes where stringent biosecurity measures restrict the frequency o...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err385

    authors: Saint Pierre C,Crossa JL,Bonnett D,Yamaguchi-Shinozaki K,Reynolds MP

    更新日期:2012-03-01 00:00:00

  • Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp.

    abstract::Xylans are the most abundant non-cellulosic polysaccharide found in plant cell walls. A diverse range of xylan structures influence tissue function during growth and development. Despite the abundance of xylans in nature, details of the genes and biochemical pathways controlling their biosynthesis are lacking. In this...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw424

    authors: Phan JL,Tucker MR,Khor SF,Shirley N,Lahnstein J,Beahan C,Bacic A,Burton RA

    更新日期:2016-12-01 00:00:00

  • Recalculating growth and defense strategies under competition: key roles of photoreceptors and jasmonates.

    abstract::The growth-defense trade-off in plant biology has gained enormous traction in the last two decades, highlighting the importance of understanding how plants deal with two of the greatest challenges for their survival and reproduction. It has been well established that in response to competition signals perceived by inf...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz237

    authors: Ballaré CL,Austin AT

    更新日期:2019-07-05 00:00:00

  • PCA22 acts as a suppressor of atrzf1 to mediate proline accumulation in response to abiotic stress in Arabidopsis.

    abstract::Proline metabolism is important for environmental responses, plant growth, and development. However, its precise roles in plant abiotic stress tolerance are not well understood. Mutants are valuable for the identification of new genes and for elucidating their roles in physiological mechanisms. We applied a suppressor...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx069

    authors: Kim AR,Min JH,Lee KH,Kim CS

    更新日期:2017-03-01 00:00:00

  • Jasmonates: biosynthesis, metabolism, and signaling by proteins activating and repressing transcription.

    abstract::The lipid-derived phytohormone jasmonate (JA) regulates plant growth, development, secondary metabolism, defense against insect attack and pathogen infection, and tolerance to abiotic stresses such as wounding, UV light, salt, and drought. JA was first identified in 1962, and since the 1980s many studies have analyzed...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erw443

    authors: Wasternack C,Song S

    更新日期:2017-03-01 00:00:00

  • KNAP2, a class I KN1-like gene is a negative marker of bud growth potential in apple trees (Malus domestica [L.] Borkh.).

    abstract::The determinism of bud bursting pattern along the 1-year-old shoot was studied at the molecular and morphological levels in the apple tree variety 'Lodi' which shows an acrotonic tendency. At the molecular level, the expression of KNAP2, which belongs to the class I KN1-like gene family, was studied. Measurements were...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erf063

    authors: Brunel N,Leduc N,Poupard P,Simoneau P,Mauget JC,Viémont JD

    更新日期:2002-11-01 00:00:00

  • Overexpression of thioredoxin m in tobacco chloroplasts inhibits the protein kinase STN7 and alters photosynthetic performance.

    abstract::The activity of the protein kinase STN7, involved in phosphorylation of the light-harvesting complex II (LHCII) proteins, has been reported as being co-operatively regulated by the redox state of the plastoquinone pool and the ferredoxin-thioredoxin (Trx) system. The present study aims to investigate the role of plast...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery415

    authors: Ancín M,Fernández-San Millán A,Larraya L,Morales F,Veramendi J,Aranjuelo I,Farran I

    更新日期:2019-02-05 00:00:00

  • alpha4-Fucosyltransferase is regulated during flower development: increases in activity are targeted to pollen maturation and pollen tube elongation.

    abstract::alpha4-Fucosylation represents a final step of protein N- glycosylation. alpha4-fucosylated N-glycans are thought to be involved in cell-to-cell communication and recognition in primates and plants. Nevertheless, in the plant life cycle, the function of alpha4-fucosylation remains largely unknown. To gain an insight i...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.373.1429

    authors: Joly C,Léonard R,Maftah A,Riou-Khamlichi C

    更新日期:2002-06-01 00:00:00

  • The light and dark sides of nitric oxide: multifaceted roles of NO in plant responses to light.

    abstract::Light drives photosynthesis and informs plants about their surroundings. Regarded as a multifunctional signaling molecule in plants, nitric oxide (NO) has been repeatedly demonstrated to interact with light signaling cascades to control plant growth, development and metabolism. During early plant development, light-tr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa504

    authors: Lopes-Oliveira PJ,Oliveira HC,Kolbert Z,Freschi L

    更新日期:2020-11-27 00:00:00

  • Involvement of Arabidopsis phospholipase D δ in regulation of ROS-mediated microtubule organization and stomatal movement upon heat shock.

    abstract::Reactive oxygen species (ROS) are plant metabolic and signaling molecules involved in responses to various external stresses, but the existence of ROS receptors and how plants respond to ROS remain largely unknown. Here we report that the plasma membrane-localized phospholipase D δ (PLDδ) protein is crucial for sensin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa359

    authors: Song P,Jia Q,Chen L,Jin X,Xiao X,Li L,Chen H,Qu Y,Su Y,Zhang W,Zhang Q

    更新日期:2020-10-22 00:00:00

  • Natural variation reveals that OsSAP16 controls low-temperature germination in rice.

    abstract::Low temperature affects seed germination in plants, and low-temperature germination (LTG) is an important agronomic trait. Natural variation of LTG has been reported in rice, but the molecular basis for this variation is largely unknown. Here we report the phenotypic analysis of LTG in 187 rice natural accessions and ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx413

    authors: Wang X,Zou B,Shao Q,Cui Y,Lu S,Zhang Y,Huang Q,Huang J,Hua J

    更新日期:2018-01-23 00:00:00

  • A methylation status analysis of the apomixis-specific region in Paspalum spp. suggests an epigenetic control of parthenogenesis.

    abstract::Apomixis, a clonal plant reproduction by seeds, is controlled in Paspalum spp. by a single locus which is blocked in terms of recombination. Partial sequence analysis of the apomixis locus revealed structural features of heterochromatin, namely the presence of repetitive elements, gene degeneration, and de-regulation....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru354

    authors: Podio M,Cáceres ME,Samoluk SS,Seijo JG,Pessino SC,Ortiz JP,Pupilli F

    更新日期:2014-12-01 00:00:00

  • Plasma membrane of Beta vulgaris storage root shows high water channel activity regulated by cytoplasmic pH and a dual range of calcium concentrations.

    abstract::Plasma membrane vesicles isolated by two-phase partitioning from the storage root of Beta vulgaris show atypically high water permeability that is equivalent only to those reported for active aquaporins in tonoplast or animal red cells (Pf=542 microm s(-1)). The values were determined from the shrinking kinetics measu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj046

    authors: Alleva K,Niemietz CM,Sutka M,Maurel C,Parisi M,Tyerman SD,Amodeo G

    更新日期:2006-01-01 00:00:00

  • Hexameric oligomerization of mitochondrial peroxiredoxin PrxIIF and formation of an ultrahigh affinity complex with its electron donor thioredoxin Trx-o.

    abstract::Mitochondria from plants, yeast, and animals each contain at least one peroxiredoxin (Prx) that is involved in peroxide detoxification and redox signalling. The supramolecular dynamics of atypical type II Prx targeted to the mitochondrion was addressed in pea. Microcalorimetric (ITC) titrations identified an extremely...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ern177

    authors: Barranco-Medina S,Krell T,Bernier-Villamor L,Sevilla F,Lázaro JJ,Dietz KJ

    更新日期:2008-01-01 00:00:00

  • Sulfate transport systems in plants: functional diversity and molecular mechanisms underlying regulatory coordination.

    abstract::Sulfate transporters are integral membrane proteins controlling the flux of sulfate (SO42-) entering the cells and subcellular compartments across the membrane lipid bilayers. Sulfate uptake is a dynamic biological process that occurs in multiple cell layers and organs in plants. In vascular plants, sulfate ions are t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz132

    authors: Takahashi H

    更新日期:2019-08-19 00:00:00

  • Transcriptional responses of Arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous Myzus persicae and oligophagous Brevicoryne brassicae.

    abstract::Plants are equipped with a range of defence mechanisms against herbivorous insects. In cruciferous species, jasmonic acid, salicylic acid, and ethylene along with glucosinolates and their hydrolysis products play important roles in plant protection and plant-insect communication. In turn, a number of herbivores have a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm043

    authors: Kusnierczyk A,Winge P,Midelfart H,Armbruster WS,Rossiter JT,Bones AM

    更新日期:2007-01-01 00:00:00

  • Alternative splicing of basic chitinase gene PR3b in the low-nicotine mutants of Nicotiana tabacum L. cv. Burley 21.

    abstract::Two unlinked semi-dominant loci, A (NIC1) and B (NIC2), control nicotine and related alkaloid biosynthesis in Burley tobaccos. Mutations in either or both loci (nic1 and nic2) lead to low nicotine phenotypes with altered environmental stress responses. Here we show that the transcripts derived from the pathogenesis-re...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw345

    authors: Ma H,Wang F,Wang W,Yin G,Zhang D,Ding Y,Timko MP,Zhang H

    更新日期:2016-10-01 00:00:00

  • Differential coupling of gibberellin responses by Rht-B1c suppressor alleles and Rht-B1b in wheat highlights a unique role for the DELLA N-terminus in dormancy.

    abstract::During the Green Revolution, substantial increases in wheat (Triticum aestivum) yields were realized, at least in part, through the introduction of the Reduced height (Rht)-B1b and Rht-D1b semi-dwarfing alleles. In contrast to Rht-B1b and Rht-D1b, the Rht-B1c allele is characterized by extreme dwarfism and exceptional...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw471

    authors: Van De Velde K,Chandler PM,Van Der Straeten D,Rohde A

    更新日期:2017-01-01 00:00:00

  • Molecular phylogeny and forms of photosynthesis in tribe Salsoleae (Chenopodiaceae).

    abstract::While many C4 lineages have Kranz anatomy around individual veins, Salsoleae have evolved the Salsoloid Kranz anatomy where a continuous dual layer of chlorenchyma cells encloses the vascular and water-storage tissue. With the aim of elucidating the evolution of C4 photosynthesis in Salsoleae, a broadly sampled molecu...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw432

    authors: Schüssler C,Freitag H,Koteyeva N,Schmidt D,Edwards G,Voznesenskaya E,Kadereit G

    更新日期:2017-01-01 00:00:00

  • Interactive effects of water stress and xylem-limited bacterial infection on the water relations of a host vine.

    abstract::Xylella fastidiosa, a xylem-limited bacterial pathogen that causes bacterial leaf scorch in its hosts, has a diverse and extensive host range among plant species worldwide. Previous work has shown that water stress enhances leaf scorch symptom severity and progression along the stem in Parthenocissus quinquefolia infe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg046

    authors: McElrone AJ,Sherald JL,Forseth IN

    更新日期:2003-01-01 00:00:00

  • Turgor, temperature and the growth of plant cells: using Chara corallina as a model system.

    abstract::Rapid changes in turgor pressure (P:) and temperature (T:) are giving new information about the mechanisms of plant growth. In the present work, single internode cells of the large-celled alga Chara corallina were used as a model for plant growth. P was changed without altering the chemical environment of the wall whi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/51.350.1481

    authors: Proseus TE,Zhu GL,Boyer JS

    更新日期:2000-09-01 00:00:00

  • A comparison of the effects of IAA and 4-Cl-IAA on growth, proton secretion and membrane potential in maize coleoptile segments.

    abstract::The physiological activity of exogenous 4-Cl-IAA, as compared to IAA, was examined in maize coleoptile segments. It was found that in this model system 4-Cl-IAA is much more active in the stimulation of elongation than IAA. Simultaneous measurements of growth and external pH indicated that administration of either IAA...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.371.1089

    authors: Karcz W,Burdach Z

    更新日期:2002-05-01 00:00:00

  • A physical, enzymatic, and genetic characterization of perturbations in the seeds of the brownseed tomato mutants.

    abstract::The brownseed mutants (bs(1), bs(2), and bs(4)) of tomato all possess dark testae and deleteriously affect seed germination speed and/or final percentage. Poor germination performance of the bs(1) but not the bs(4) mutant, was due to greater impediment to radicle egress. Testa toughening (bs(1)) was prevented by dryin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh112

    authors: Downie AB,Dirk LM,Xu Q,Drake J,Zhang D,Dutt M,Butterfield A,Geneve RR,Corum JW 3rd,Lindstrom KG,Snyder JC

    更新日期:2004-05-01 00:00:00

  • Ethylene-induced Arabidopsis hypocotyl elongation is dependent on but not mediated by gibberellins.

    abstract::Ethylene, or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC), can stimulate hypocotyl elongation in the light. It is questioned whether gibberellins (GAs) play a role in this response. Tests with light of different wavelengths demonstrated that the ethylene response depends on blue light and functional crypt...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm288

    authors: Vandenbussche F,Vancompernolle B,Rieu I,Ahmad M,Phillips A,Moritz T,Hedden P,Van Der Straeten D

    更新日期:2007-01-01 00:00:00

  • Expression of characteristics of ammonium nutrition as affected by pH of the root medium.

    abstract::To study the effect of root-zone pH on characteristic responses of NH4+ -fed plants, soybeans (Glycine max¿L.¿ Merr. cv. Ransom) were grown in flowing solution culture for 21 d on four sources of N (1.0 mol m-3 NO3-, 0.67 mol m-3 NO3- plus 0.33 mol m-3 NH4+, 0.33 mol m-3 NO3- plus 0.67 mol m-3 NH4+, and 1.0 mol m-3 NH...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/42.2.189

    authors: Chaillou S,Vessey JK,Morot-Gaudry JF,Raper CD Jr,Henry LT,Boutin JP

    更新日期:1991-02-01 00:00:00

  • Cross-talk between reactive oxygen species and polyamines in regulation of ion transport across the plasma membrane: implications for plant adaptive responses.

    abstract::Many stresses are associated with increased accumulation of reactive oxygen species (ROS) and polyamines (PAs). PAs act as ROS scavengers, but export of putrescine and/or PAs to the apoplast and their catabolization by amine oxidases gives rise to H2O2 and other ROS, including hydroxyl radicals ((•)OH). PA catabolizat...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/ert423

    authors: Pottosin I,Velarde-Buendía AM,Bose J,Zepeda-Jazo I,Shabala S,Dobrovinskaya O

    更新日期:2014-03-01 00:00:00

  • β-Carboxysome bioinformatics: identification and evolution of new bacterial microcompartment protein gene classes and core locus constraints.

    abstract::Carboxysomes are bacterial microcompartments (BMCs) that enhance CO2 fixation in all cyanobacteria. Structurally, carboxysome shell proteins are classified according to the type of oligomer formed: hexameric (BMC-H), trimeric (BMC-T) and pentameric (BMC-P) proteins. To understand the forces driving the evolution of th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx115

    authors: Sommer M,Cai F,Melnicki M,Kerfeld CA

    更新日期:2017-06-01 00:00:00

  • Abscisic acid prevents the coalescence of protein storage vacuoles by upregulating expression of a tonoplast intrinsic protein gene in barley aleurone.

    abstract::Tonoplast intrinsic proteins (TIPs) are integral membrane proteins that are known to function in plants as aquaporins. Here, we propose another role for TIPs during the fusion of protein storage vacuoles (PSVs) in aleurone cells, a process that is promoted by gibberellic acid (GA) and prevented by abscisic acid (ABA)....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru467

    authors: Lee SE,Yim HK,Lim MN,Yoon Is,Kim Jh,Hwang YS

    更新日期:2015-03-01 00:00:00

  • Characterization of ion channel modulator effects on ABA- and malate-induced stomatal movements: strong regulation by kinase and phosphatase inhibitors, and relative insensitivity to mastoparans.

    abstract::In the present study abscisic acid-induced stomatal closing, and malate effects on stomatal apertures were analysed in the presence of guard cell ion channel regulators. A recent study has suggested that abscisic acid (ABA) activation of protein kinases and/or inhibition of protein phosphatases may be central to activ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/48.Special_Issue.539

    authors: Esser JE,Liao YJ,Schroeder JI

    更新日期:1997-03-01 00:00:00