当前位置： SCI文献检索 > JOURNAL OF ASSISTED REPRODUCTION AND GENETICS期刊下所有文献 > Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker.

Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker.

Abstract：

PURPOSE:To understand the molecular basis of sperm-motility and to identify related novel motility biomarkers. METHODS:Two-dimensional electrophoresis (2DE) followed by Reverse-phase-nano-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-nano-HPLC-ESI-MS/MS) were applied to establish the human sperm proteome. Then the sperm proteome of moderate-motile human sperm fraction and that of good-motile human sperm fraction from pooled spermatozoa of forty normozoospermic donors (Group 1 subjects) were compared to identify the dysregulated proteins. Among these down-regulated proteins, Protein tyrosine phosphatase non-receptor type 14 (PTPN14) was chosen to reconfirm by Western blotting and semi-quantitative reverse transcription polymerase chain reaction. For clinical application, Western blotting and real-time reverse transcription polymerase chain reaction was performed to compare the expression level of PTPN14 in (Group 2 subjects) nine normozoospermic controls and thirty-three asthenozoospermic patients (including 21 mild asthenozoospermic cases and 12 severe cases). Finally, bioinformatic tools prediction and immunofluorescence assay were performed to elucidate the potential localization of PTPN14. RESULTS:The expression levels of three proteins were observed to be lower in the moderate-motile sperm fraction than in good-motile sperm of group 1 subjects. Among three proteins with persistent down-regulation in the moderate-motile sperm, we reconfirmed that the expression level of PTPN14 was significantly lower in both mRNA and protein levels from the moderate-motile sperm fraction. Further, down-regulation of PTPN14 was found at the translational and transcriptional level in the asthenozoospermic men. Finally, Bioinformatic tools prediction and immunofluorescence assay showed that PTPN14 maybe predominantly localized at the mitochondria in the midpiece of human ejaculated sperm. CONCLUSIONS:Proteomics tools were applied to identify three possible sperm motility-related proteins. Among these proteins, PTPN14 was highly likely a novel sperm-motility biomarker and a potential mitochondrial protein.

journal_name

J Assist Reprod Genet

journal_title

Journal of assisted reproduction and genetics

authors

Chao HC,Chung CL,Pan HA,Liao PC,Kuo PL,Hsu CC

doi

10.1007/s10815-011-9602-0

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

851-61

issue

9

eissn

1058-0468

issn

1573-7330

journal_volume

28

pub_type

杂志文章

相关文献

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS文献大全
  • Reflections on the cost-effectiveness of recombinant FSH in assisted reproduction. The clinician's perspective.

    abstract：PURPOSE:To analyze the relative cost-effectiveness of recombinant FSH (rFSH) and urinary FSH (uFSH) in assisted reproduction techniques (ART). METHODS:Calculation of the average cost-effectiveness ratio and the incremental cost-effectiveness ratio to compare costs and effects (pregnancy rates) of the two therapeutic o...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1023/a:1026501821849

    authors： Balasch J,Barri PN

    更新日期：2001-02-01 00:00:00

  • Complex preimplantation genetic tests for Robertsonian translocation, HLA, and X-linked hyper IgM syndrome caused by a novel mutation of CD40LG gene.

    abstract：PURPOSE:To perform complex preimplantation genetic tests (PGT) for aneuploidy screening, Robertsonian translocation, HLA-matching, and X-linked hyper IgM syndrome (XHIGM) caused by a novel mutation c.156 G>T of CD40LG gene. METHODS:Reverse transcription PCR (RT-PCR) and Sanger sequencing were carried out to confirm th...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-020-01846-y

    authors： Huang S,Niu Y,Li J,Gao M,Zhang Y,Yan J,Ma S,Gao X,Gao Y

    更新日期：2020-08-01 00:00:00

  • Capacitation IVM improves cumulus function and oocyte quality in minimally stimulated mice.

    abstract：PURPOSE:Oocyte in vitro maturation (IVM) is a patient-friendly reproductive technology but lower success rates than IVF have limited its uptake. Capacitation-IVM (CAPA-IVM) is an innovative new IVM system currently undergoing clinical evaluation. This study aimed to determine temporal effects of the pre-IVM phase of CA...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-019-01610-x

    authors： Zhao Y,Liao X,Krysta AE,Bertoldo MJ,Richani D,Gilchrist RB

    更新日期：2020-01-01 00:00:00

  • Growth factor/cytokine secretion by a permanent human endometrial cell line with embryotrophic properties.

    abstract：OBJECTIVE:Our goal was to identify and quantitate growth factors and cytokines actively secreted by a permanent human endometrial cell line with embryotrophic properties and to determine if cell lines enhancing embryo development secrete common growth factors and/or cytokines. DESIGN:Culture medium conditioned by this...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02066606

    authors： Desai NN,Goldfarb JM

    更新日期：1996-08-01 00:00:00

  • Epidermal growth factor (EGF) receptor localization in cultured human granulosa lutein cells and the stimulation of progesterone production by EGF and transforming growth factor-alpha (TGF-alpha).

    abstract：PURPOSE:The purpose of this study was to investigate the expression of EGF receptor (EGF-R) in human granulosa cells undergoing luteinization and progesterone production by these cells in response to EGF an TGF-alpha alone or in combination with luteinizing hormone (LH). Granulosa cells were obtained from IF patients f...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02212900

    authors： Tekpetey FR,Daniel SA,Yuzpe A

    更新日期：1995-11-01 00:00:00

  • Prognostic implication of apoptosis in human luteinized granulosa cells during IVF-ET.

    abstract：PURPOSE:To investigate the prognostic significance of apoptosis in granulosa cells recovered from patients participating in IVF-ET. METHODS:Seventy-three women underwent ovarian hyperstimulation for IVF and embryo transfer. After follicle aspiration, recovered granulosa cells were stained by BCL2 monoclonal antibody w...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1023/a:1015319617598

    authors： Suh CS,Jee BC,Choi YM,Kim JG,Lee JY,Moon SY,Kim SH

    更新日期：2002-05-01 00:00:00

  • Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis.

    abstract：PURPOSE:To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy. METHODS:This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-014-0209-0

    authors： Hosseinifar H,Sabbaghian M,Nasrabadi D,Modarresi T,Dizaj AV,Gourabi H,Gilani MA

    更新日期：2014-06-01 00:00:00

  • Effect of Percoll wash on sperm motion parameters and subsequent fertility in intrauterine insemination cycles.

    abstract：PURPOSE:This study examined sperm motion parameters as measured by computerized automated semen analysis before and after a Percoll wash and determined if differences in any parameter were correlated with fertility subsequent to intrauterine insemination (IUI). RESULTS:Total motile sperm decreased following the washin...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02214129

    authors： Swanson ML,Collins JM,Freiman SF,Dubin NH

    更新日期：1995-01-01 00:00:00

  • Influence of pituitary suppression with triphasic or monophasic oral contraceptives on the outcome of in vitro fertilization and embryo transfer.

    abstract：PURPOSE:To compare the clinical outcome of IVF treatment after pituitary suppression with two different oral contraceptives (OCs). METHODS:65 patients who received IVF treatment was classified into 2 groups based on the difference of OCs they used for pituitary suppression before ovarian hyperstimulation. Group 1 incl...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-006-9056-y

    authors： Chung MT,Tsai YC,Chen SH,Loo TC,Tang HH,Lin LY

    更新日期：2006-07-01 00:00:00

  • FoxO transcription factors 1 regulate mouse preimplantation embryo development.

    abstract：PURPOSE:The aim of the present study is to investigate role of FoxO transcription factors in preimplantation embryo development by knocking down FoxO1, FoxO3, and FoxO4 genes and also to assess cell cycle arrest related proteins, p53 and p21, and apoptosis-related proteins, fas ligand (FASL), and cleaved caspase 3. ME...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-019-01555-1

    authors： Kuscu N,Gungor-Ordueri NE,Sozen B,Adiguzel D,Celik-Ozenci C

    更新日期：2019-10-01 00:00:00

  • Temporal expression of cumulus cell marker genes during in vitro maturation and oocyte developmental competence.

    abstract：PURPOSE:Cumulus cells (CC) play important roles in oocyte development and cumulus expressed genes can be used as markers for oocyte quality. This study aimed to investigate temporal changes in the expression of cumulus marker genes during oocyte maturation as possible biomarkers of embryo developmental competence in ov...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-017-0998-z

    authors： Dhali A,Javvaji PK,Kolte AP,Francis JR,Roy SC,Sejian V

    更新日期：2017-11-01 00:00:00

  • Pre-implantation developmental potential from in vivo and in vitro matured mouse oocytes: a cytoskeletal perspective on oocyte quality.

    abstract：PURPOSE:In the present study, fertilization and developmental potential of mouse oocytes matured in different conditions were tested. The efficiency of in vitro fertilization (IVF), pre-implantation development and some important aspects of cytokinesis during early cleavages are discussed. METHODS:In vivo matured (IVO...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-014-0363-4

    authors： Sanfins A,Plancha CE,Albertini DF

    更新日期：2015-01-01 00:00:00

  • Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures.

    abstract：PURPOSE:We investigated whether enzymatically fabricated hyaluronan (HA) microcapsules were feasible for use in the cryopreservation of a small number of sperm. METHODS:HA microcapsules were fabricated using a system of water-immiscible fluid under laminar flow. Three sperm were injected into a hollow HA microcapsule ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-016-0656-x

    authors： Tomita K,Sakai S,Khanmohammadi M,Yamochi T,Hashimoto S,Anzai M,Morimoto Y,Taya M,Hosoi Y

    更新日期：2016-04-01 00:00:00

  • Serum progesterone levels greater than 20 ng/dl on day of embryo transfer are associated with lower live birth and higher pregnancy loss rates.

    abstract：BACKGROUND:Progesterone (P4) is essential for support of the endometrium and implantation of an embryo in the normal menstrual cycle. In programed frozen embryo transfer cycles using exogenous P4 is necessary, as the endogenous production of P4 requires a functioning corpus luteum that is not present in programed cycle...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-015-0546-7

    authors： Kofinas JD,Blakemore J,McCulloh DH,Grifo J

    更新日期：2015-09-01 00:00:00

  • Cyclooxygenase (COX)-2 and granulosa cell apoptosis in vitro.

    abstract：PURPOSE:C-myc was studied in cyclooxygenase (COX)-2 associated granulosa cell apoptosis, METHODS:Granulosa cells (N = 5 cases) were incubated for 24 h in either 1 or 50 microM COX-2 inhibitor, 1 or 50 microM COX-1/COX-2 inhibitor, negative or positive controls Single primer polymerase chain reaction of c-myc exon 1 we...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1023/a:1021215216246

    authors： Caffrey AS,Patton WC,Corselli JU,Swensen RE,King A,Chan PJ

    更新日期：2002-12-01 00:00:00

  • The effect of intrafollicular IGF 1 and IGFBP 3 on IVF outcome in patients using different gonadotropins: a prospective study.

    abstract：AIM:To investigate follicular fluid IGF 1, IGFBP 3 and their effects on IVF outcome in patients using different gonadotropins. MATERIALS AND METHODS:This prospective study was performed among 92 IVF patients who were categorized according to IVF pregnancy outcome. Two groups were formed as Group 1 : pregnant, Group 2:...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-011-9543-7

    authors： Genc G,Yilmaz N,Uygur D,Dogan M,Mollamahmutoglu L

    更新日期：2011-05-01 00:00:00

  • Do estrogen receptor alpha polymorphisms have any impact on the outcome in an ART program?

    abstract：PURPOSE:To investigate two of the most studied estrogen receptor alpha polymorphisms (PvuII and XbaI) in combination, in order to evaluate their impact on an ART program outcome. METHODS:203 normally ovulating women who underwent IVF or ICSI treatment were genotyped for PvuII and XbaI polymorphisms in ESR1 intron 1 us...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-013-9971-7

    authors： Anagnostou E,Malamas F,Mavrogianni D,Dinopoulou V,Drakakis P,Kallianidis K,Loutradis D

    更新日期：2013-04-01 00:00:00

  • Effects of supplement therapy on sperm parameters, protamine content and acrosomal integrity of varicocelectomized subjects.

    abstract：PURPOSE:Surgical repair of varicocele has long been a procedure to correct spermatogenesis. However, the outcome has been reported to be inadequate. We combined varicocelectomy with supplement therapy to evaluate the concurrent effect of these procedures. METHODS:A prospective randomized controlled study was undertake...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s10815-013-9961-9

    authors： Azizollahi G,Azizollahi S,Babaei H,Kianinejad M,Baneshi MR,Nematollahi-mahani SN

    更新日期：2013-04-01 00:00:00

  • Pregnancy after endometrial scratching in infertile couples undergoing ovulation induction and intrauterine insemination cycles-a randomized controlled trial.

    abstract：PURPOSE:To study the effect of endometrial scratching in infertile couples undergoing ovulation induction and intrauterine insemination (IUI) cycles. METHODS:A prospective randomized controlled trial was conducted in the Department of Obstetrics and Gynaecology, AIIMS, New Delhi, India. One hundred forty-four women wi...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s10815-017-0949-8

    authors： Goel T,Mahey R,Bhatla N,Kalaivani M,Pant S,Kriplani A

    更新日期：2017-08-01 00:00:00

  • Expression of cell cycle regulatory proteins (p53, pRb) in the human female genital tract.

    abstract：PURPOSE:Recent studies have shown that proliferation and differentiation of various cell types is regulated by cell-cycle-related proteins, such as protein p53 and retinoblastoma protein pRb. METHODS:Three monoclonal antibodies to p53 (PAb240, PAb421, and PAb1801) and 3H9 monoclonal antibody to pRb were utilized for l...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02211381

    authors： Bukovsky A,Caudle MR,Keenan JA,Wimalasena J,Foster JS,Upadhyaya NB,van Meter SE

    更新日期：1995-02-01 00:00:00

  • "One for Sorrow, Two for Joy?": American embryo transfer guideline recommendations, practices, and outcomes for gestational surrogate patients.

    abstract：:In January 2016, Melissa Cook, a California gestational surrogate experiencing a multiple-birth pregnancy following the in vitro fertilization (IVF) transfer of three embryos comprised of donor eggs and sperm provided by the intended father, went to the media when the intended father requested that she undergo a fetal...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s10815-017-0885-7

    authors： White PM

    更新日期：2017-04-01 00:00:00

  • The significance of an early (premature) rise of plasma progesterone in in vitro fertilization cycles induced by a "long protocol" of gonadotropin releasing hormone analogue and human menopausal gonadotropins.

    abstract：OBJECTIVE:Our purpose was to assess and clarify the mechanism of whether an early progesterone rise in cycles with gonadotropin-releasing hormone agonist (GnRH-a) is associated with an impairment of IVF outcome. METHODS:Seven hundred eighty-six cycles were induced with GnRH-a and human menopausal gonodotropin (hMG) ("...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02065937

    authors： Shulman A,Ghetler Y,Beyth Y,Ben-Nun I

    更新日期：1996-03-01 00:00:00

  • Negative effect of P72 polymorphism on p53 gene in IVF outcome in patients with repeated implantation failure and pregnancy loss.

    abstract：PURPOSE:Investigate whether R72P on p53 gene polymorphism has a higher prevalence among women with a history of recurrent implantation failure (RIF) and pregnancy loss (RPL) and its influence in their IVF cycle outcome. MATERIAL AND METHODS:p53 polymorphism R72P has been studied in 181 women. The control group include...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-013-0147-2

    authors： Lledo B,Turienzo A,Ortiz JA,Morales R,Ten J,Llácer J,Bernabeu R

    更新日期：2014-02-01 00:00:00

  • Localization of parathyroid hormone-related protein in the preimplantation mouse embryo is associated with events of blastocyst hatching.

    abstract：PURPOSE:To determine the pattern of expression of parathyroid hormone-related protein (PTHrP) and its receptor, parathyroid hormone receptor 1 (PTHR1), in mouse embryos in different stages of preimplantation development. METHODS:Embryos were cultured from the pronuclear zygote stage and harvested as 2-cell, 4-cell and...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-013-0094-y

    authors： Erbach GT,Biggers JD,Manning PC,Nowak RA

    更新日期：2013-08-01 00:00:00

  • Treatment history and outcome of 24 deliveries worldwide after autotransplantation of cryopreserved ovarian tissue, including two new Danish deliveries years after autotransplantation.

    abstract：PURPOSE:To report another two successful pregnancies and deliveries resulting from autotransplanted cryopreserved ovarian tissue several years after the autotransplantation procedure took place. Further, to review the literature on the treatment history, number of live births and their outcome so far reported worldwide...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-014-0331-z

    authors： Macklon KT,Jensen AK,Loft A,Ernst E,Andersen CY

    更新日期：2014-11-01 00:00:00

  • Ratio of progesterone-to-number of follicles as a prognostic tool for in vitro fertilization cycles.

    abstract：PURPOSE:To establish a ratio of the P level to the number of follicles (P/F ratio) on the day of human chorionic gonadotropin (hCG) administration and to evaluate whether this ratio is associated with in vitro fertilization (IVF) outcome. METHODS:This study was conducted between January 2012 and June 2013. A total of ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-015-0487-1

    authors： Roque M,Valle M,Sampaio M,Geber S,Checa MA

    更新日期：2015-06-01 00:00:00

  • Gene-environment interaction among GSTT1, PON2 polymorphisms and organic solvents on gestational age in a Chinese women cohort.

    abstract：PURPOSE:To investigate interactions on gestational age among two environmental risk factors and four maternal genetic polymorphisms: organic solvents, passive smoking, CYP1A1 rs4646903 (MspI), EPHX1 rs2234922 (His139Arg), GSTT1 and PON2 rs12026 (Ala148Gly). METHODS:A pregnant women cohort was conducted at Beijing Yans...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-014-0256-6

    authors： Li S,Fang K,Wang W,Hu Y,Chen D

    更新日期：2014-07-01 00:00:00

  • Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes.

    abstract：PURPOSE:We studied the influence of aging, hyaluronidase removal of the cumulus, and microinjection on the sperm binding potential of human oocytes under intact zona assay conditions to determine the safe use of unstored aged unfertilized ICSI oocytes on zona binding tests. Results were also compared with those for age...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02765778

    authors： Barros A,Sousa M,Silva J,Almeida V,Rocha E

    更新日期：1997-02-01 00:00:00

  • Preimplantation genetic diagnosis of inherited cancer: familial adenomatous polyposis coli.

    abstract：PURPOSE:Our purpose was to achieve preimplantation genetic diagnosis (PGD) of the dominant cancer predisposition syndrome, familial adenomatous polyposis coli (FAPC), as an alternative to prenatal diagnosis. METHODS:The affected patient was superovulated and oocytes were retrieved and fertilized by intracytoplasmic sp...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1023/a:1023008921386

    authors： Ao A,Wells D,Handyside AH,Winston RM,Delhanty JD

    更新日期：1998-03-01 00:00:00

  • The effects of E2 supplementation from the early proliferative phase to the late secretory phase of the endometrium in hMG-stimulated IVF-ET.

    abstract：PURPOSE:Our purpose was to determine if pregnancy rates (PRs) for hMG (human menopausal gonadotropin)-stimulated IVF-ET (in vitro fertilization--embryo transfer) can be increased by estradiol (E2) supplementation from the early proliferative phase to the late secretory phase of the endometrium. METHOD:Eighty-one infer...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1023/a:1009445913156

    authors： Jung H,Roh HK

    更新日期：2000-01-01 00:00:00