Abstract：
PURPOSE:The aim of the present study is to investigate role of FoxO transcription factors in preimplantation embryo development by knocking down FoxO1, FoxO3, and FoxO4 genes and also to assess cell cycle arrest related proteins, p53 and p21, and apoptosis-related proteins, fas ligand (FASL), and cleaved caspase 3. METHODS:Knockdown of FoxOs using siRNA was confirmed utilizing RT-PCR and qRT-PCR in gene level and using immunofluorescence in protein level. Following knockdown of FoxO1, FoxO3, and FoxO4 in two-cell mouse embryos with or without resveratrol treatment; developmental competence of embryos and expression patterns of SIRT1, p53, p21, FASL, and CLEAVED CASPASE 3 proteins in embryos by immunofluorescence were assessed after 48 h. ROS levels were measured in knockdown embryos. Terminal deoxynucleotidyl transferase dUTP nick end labeling assay was used to determine resveratrol dose. RESULTS:Successful knockdown of FoxO genes in mouse embryos utilizing a non-invasive siRNA method was achieved. Significantly, knockdown of FoxO genes impaired preimplantation embryo development which cannot be prevented by resveratrol treatment. Immunofluorescence results showed that resveratrol could protect embryos from cell cycle arrest and apoptosis. FOXO proteins regulate apoptosis and cell cycle related proteins in mouse preimplantation embryos. Moreover, there might be an autofeedback mechanism where FOXO1, FOXO3, and FOXO4 regulate SIRT1 protein expression. CONCLUSIONS:These results suggest that FOXO transcription factors could contribute to mouse preimplantation embryo development, and it remains to investigate whether they have crucial roles in human preimplantation embryo and infertility.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Kuscu N,Gungor-Ordueri NE,Sozen B,Adiguzel D,Celik-Ozenci Cdoi
10.1007/s10815-019-01555-1subject
Has Abstractpub_date
2019-10-01 00:00:00pages
2121-2133issue
10eissn
1058-0468issn
1573-7330pii
10.1007/s10815-019-01555-1journal_volume
36pub_type
杂志文章abstract：:The incorporation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing into patient care algorithms has been proposed to mitigate risk. However, the two main professional societies for human reproduction (ESHRE and ASRM) appear divergent on their clinical utility and whether they should be adopted. ...
journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
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pub_type： 杂志文章
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journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,评审
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journal_title：Journal of assisted reproduction and genetics
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更新日期：2011-09-01 00:00:00
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更新日期：2013-11-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02065937
更新日期：1996-03-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
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更新日期：2014-07-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
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更新日期：2020-02-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
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更新日期：2006-07-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02744938
更新日期：1998-08-01 00:00:00
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更新日期：2020-11-16 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
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更新日期：1998-02-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
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更新日期：2002-10-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
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