当前位置： SCI文献检索 > JOURNAL OF ASSISTED REPRODUCTION AND GENETICS期刊下所有文献 > Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes.

Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes.

Abstract：

PURPOSE:We studied the influence of aging, hyaluronidase removal of the cumulus, and microinjection on the sperm binding potential of human oocytes under intact zona assay conditions to determine the safe use of unstored aged unfertilized ICSI oocytes on zona binding tests. Results were also compared with those for aged IVF oocytes under the same conditions. METHODS:To avoid the large variation in sperm binding scores, we compared the ratios of the number of sperm bound to nontreated versus to treated oocytes. Treated oocytes were those whose zonae were exposed to hyaluronidase, an agent that decreases sperm binding. Experimental groups were fresh oocytes, experimentally aged fresh oocytes, fresh oocytes whose cumulus was removed either mechanically or with hyaluronidase, aged ICSI oocytes, and aged IVF oocytes. RESULTS:Statistical analysis within and between groups showed that aging, previous insemination, cumulus removal by hyaluronidase, and microinjection did not affect the sperm binding potential of human oocytes. CONCLUSIONS:As the zona binding ability of fresh and unfertilized aged ICSI oocytes is similar, they can be used safely in zona assays.

journal_name

J Assist Reprod Genet

journal_title

Journal of assisted reproduction and genetics

authors

Barros A,Sousa M,Silva J,Almeida V,Rocha E

doi

10.1007/BF02765778

subject

Has Abstract

pub_date

1997-02-01 00:00:00

pages

97-101

issue

2

eissn

1058-0468

issn

1573-7330

journal_volume

14

pub_type

杂志文章

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