当前位置： SCI文献检索 > JOURNAL OF ASSISTED REPRODUCTION AND GENETICS期刊下所有文献 > Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis.

Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis.

Abstract：

PURPOSE:To study the effect of α-linolenic acid (ALA) on meiotic maturation, mRNA abundance of apoptosis-related (Bax and Bcl-2) molecules, and blastocyst formation in ovine oocytes. METHODS:A preliminary experiment was conducted to analyze the concentration of ALA in "small" (≤2 mm) and "large" (≥6 mm) follicles using gas chromatography/mass spectrometry analysis. The concentration of ALA in small and large follicles was determined to be in a range of 75.4 to 125.7 μM, respectively. In vitro maturation (IVM) of oocyte was then performed in presence of 0 (control), 10 (ALA-10), 50 (ALA-50), 100 (ALA-100), and 200 (ALA-200) μM of ALA. Meiotic maturation and mRNA abundance of Bax, and Bcl-2 genes was evaluated after 24 h of IVM. The embryonic cleavage and blastocyst formation following parthenogenetic activation were also determined for each group. RESULTS:The highest concentration of ALA (ALA-200) decreased the oocyte maturation rate compared with the control group. Analysis of apoptosis-related genes in oocytes after IVM revealed lesser transcript abundances for Bax gene, and higher transcript abundances for Bcl-2 gene in ALA-treated oocytes as compared with the control oocytes. In term of cleavage rate (considered as 2-cell progression), we did not observe any differences among the groups. However, ALA-100 group promoted more blastocyst formation as compared with the control group. CONCLUSION:Our results suggested that ALA treatment during IVM had a beneficial effect on developmental competence of ovine oocytes by increasing the blastocyst formation and this might be due to the altered abundance of apoptosis-regulatory genes.

journal_name

J Assist Reprod Genet

journal_title

Journal of assisted reproduction and genetics

authors

Veshkini A,Asadi H,Khadem AA,Mohammadi-Sangcheshmeh A,Khazabi S,Aminafshar M,Deldar H,Soleimani M,Cinar MU

doi

10.1007/s10815-015-0439-9

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

653-9

issue

4

eissn

1058-0468

issn

1573-7330

journal_volume

32

pub_type

杂志文章

相关文献

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS文献大全
  • The effects of lignocaine on human sperm motility.

    abstract：OBJECTIVE:The purpose of this study was to assess the motility of human sperm incubated with various concentrations of lignocaine. METHODS:Eleven semen samples with a sperm density greater than or equal to 20 x 10(6) ml and progressive motility greater than or equal to 40% were prepared using a swim-up technique. Aliq...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF01203827

    authors： Bennett SJ,Bolton V,Parsons J

    更新日期：1992-06-01 00:00:00

  • Closed vitrification of human oocytes and blastocysts: outcomes from a series of clinical cases.

    abstract：PURPOSE:High survival rates and clinical outcomes similar to those from fresh oocytes and blastocysts have been observed with open oocyte vitrification systems. It has been suggested that the extremely fast cooling rates that are only achieved with open systems are necessary for human oocyte and blastocyst vitrificatio...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-016-0748-7

    authors： Gook DA,Choo B,Bourne H,Lewis K,Edgar DH

    更新日期：2016-09-01 00:00:00

  • Capacitation IVM improves cumulus function and oocyte quality in minimally stimulated mice.

    abstract：PURPOSE:Oocyte in vitro maturation (IVM) is a patient-friendly reproductive technology but lower success rates than IVF have limited its uptake. Capacitation-IVM (CAPA-IVM) is an innovative new IVM system currently undergoing clinical evaluation. This study aimed to determine temporal effects of the pre-IVM phase of CA...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-019-01610-x

    authors： Zhao Y,Liao X,Krysta AE,Bertoldo MJ,Richani D,Gilchrist RB

    更新日期：2020-01-01 00:00:00

  • Does patient semen quality alter during an in vitro fertilization (IVF) program in a manner that is clinically significant when specific counseling is in operation?

    abstract：PURPOSE:It has been shown that the stress of infertility may impair semen quality. Whether counseling can attenuate this affect is unproven. This study examines, in an IVF program setting, where specific counseling is in operation, whether semen collected on the day of oocyte recovery is significantly different from th...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02211806

    authors： Drudy L,Harrison R,Verso J,Cottell E,Kondaveeti U,Barry-Kinsella C,Gordon A

    更新日期：1994-04-01 00:00:00

  • Transcripts encoding free radical scavengers in human granulosa cells from primordial and primary ovarian follicles.

    abstract：PURPOSE:To study the presence and distribution of genes encoding free radical scavengers in human granulosa cells from primordial and primary ovarian follicles. METHODS:A class comparison study on existing granulosa cell transcriptome from primordial (n = 539 follicles) and primary (n = 261) follicles donated by three...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-018-1240-3

    authors： Ernst EH,Lykke-Hartmann K

    更新日期：2018-10-01 00:00:00

  • Analysis of the sex ratio in preimplantation embryos from B6.K1 and B6.K2 Ped gene congenic mice.

    abstract：PURPOSE:The mouse preimplantation embryo development (Ped) gene product, Qa-2, which is the homolog of human HLA-G, influences the rate of preimplantation embryonic development and overall reproductive success. The sex ratio in preimplantation embryos from Ped gene congenic mice was examined in order to determine wheth...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-006-9046-0

    authors： Byrne MJ,Newmark JA,Warner CM

    更新日期：2006-07-01 00:00:00

  • Combined intrauterine triplet and ectopic pregnancy following pronuclear embryo transfer in a patient with elevated serum progesterone during ovulation induction.

    abstract：:We present a case of heterotopic pregnancy occurring after ET of pronuclear-stage embryos. This case demonstrates that pronuclear embryos have a good implantation potential and suggests that the potentially adverse effect of high follicular-phase serum progesterone levels on endometrial development might be offset by ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF01212937

    authors： Smith S,Scott L,Hosid S

    更新日期：1993-10-01 00:00:00

  • Association study between single-nucleotide variants rs12097821, rs2477686, and rs10842262 and idiopathic male infertility risk in Serbian population with meta-analysis.

    abstract：PURPOSE:A genome-wide association study conducted in the Han Chinese population identified three single nucleotide variants rs12097821, rs2477686, and rs10842262 as being significantly associated with non-obstructive azoospermia. Our aim was to evaluate the possible association between these susceptibility loci and idi...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-020-01920-5

    authors： Nemanja V,Zorana D,Nevena K,Suzana M,Ivan V,Branko B,Mirka D,Dusanka SP,Goran B

    更新日期：2020-11-01 00:00:00

  • A specific and quick gene expression study in mouse ES cells.

    abstract：PURPOSE:To study gene expression at single embryonic stem cell colony levels with a new RT-PCR protocol. METHODS:Forty-five mouse ES cell colonies were retrieved at the 5th, 10th, 15th, 20th and 25th passages. The pluripotent state was analyzed for OCT-4 and Nanog, and beta-actin as a control for the presence of templ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-007-9141-x

    authors： Tavares RL,Xu K,Zhang C,de Freitas V

    更新日期：2007-08-01 00:00:00

  • In vitro DNA fluorescence after in vitro fertilization (IVF) failure.

    abstract：PURPOSE:A pilot study was performed to test the diagnostic value of in vitro DNA fluorescence in oocytes that failed to fertilize after IVF. Ten patients with a cleavage rate less than 20% after IVF were included. RESULTS:Uncleaved oocytes were observed by fluorescence microscopy after incubation with the DNA fluoresc...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF01204255

    authors： Van Wissen B,Eisenberg C,Debey P,Pennehouat G,Auger J,Bomsel-Helmreich O

    更新日期：1992-12-01 00:00:00

  • Prognostic implication of apoptosis in human luteinized granulosa cells during IVF-ET.

    abstract：PURPOSE:To investigate the prognostic significance of apoptosis in granulosa cells recovered from patients participating in IVF-ET. METHODS:Seventy-three women underwent ovarian hyperstimulation for IVF and embryo transfer. After follicle aspiration, recovered granulosa cells were stained by BCL2 monoclonal antibody w...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1023/a:1015319617598

    authors： Suh CS,Jee BC,Choi YM,Kim JG,Lee JY,Moon SY,Kim SH

    更新日期：2002-05-01 00:00:00

  • Localization of parathyroid hormone-related protein in the preimplantation mouse embryo is associated with events of blastocyst hatching.

    abstract：PURPOSE:To determine the pattern of expression of parathyroid hormone-related protein (PTHrP) and its receptor, parathyroid hormone receptor 1 (PTHR1), in mouse embryos in different stages of preimplantation development. METHODS:Embryos were cultured from the pronuclear zygote stage and harvested as 2-cell, 4-cell and...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-013-0094-y

    authors： Erbach GT,Biggers JD,Manning PC,Nowak RA

    更新日期：2013-08-01 00:00:00

  • Does subtle progesterone rise on the day of HCG affect pregnancy rate in long agonist ICSI cycles?

    abstract：PURPOSE:To evaluate the impact of subtle progesterone (P4) rise on the day of HCG on pregnancy outcome in ICSI patients stimulated with long agonist protocol. METHODS:One hundred forty-nine consecutive controlled ovarian hyperstimulation cycles for ICSI using long luteal agonist protocol. RESULTS:Mean serum progester...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-009-9309-7

    authors： Saleh HA,Omran MS,Draz M

    更新日期：2009-05-01 00:00:00

  • A case of chimerism-induced paternity confusion: what ART practitioners can do to prevent future calamity for families.

    abstract：:In the fertility clinic setting, a negative DNA paternity test result usually suggests a sample mix-up likely occurred at the testing company or in the clinic. However, we report a case where, despite repeat negative paternity test results, the alleged father (referred to as "the proband") was confirmed to be the baby...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-017-1064-6

    authors： Sheets KM,Baird ML,Heinig J,Davis D,Sabatini M,Starr DB

    更新日期：2018-02-01 00:00:00

  • Y chromosome structural variation in infertile men detected by targeted next-generation sequencing.

    abstract：PURPOSE:To provide a validated method to identify copy number variation (CNV) in regions of the Y chromosome of infertile men by next-generation sequencing (NGS). METHODS:Semen analysis was used to determine the quality of semen and diagnose infertility. Deletion of the azoospermia factor (AZF) region in the Y chromos...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-020-02031-x

    authors： Liu X,Zhang H,Zhang X,Zhang H,Jiang Y,Liu R,Fei J,Wang Y,Yu Y

    更新日期：2021-01-16 00:00:00

  • Development of a high-yield technique to isolate spermatogonial stem cells from porcine testes.

    abstract：PURPOSE:To date, the methods available for isolating spermatogonial stem cells (SSCs) from porcine testicular cells have a low efficiency of cell separating. Therefore, we tried to develop a novel isolation technique with a high-yield cell separating ability to isolate SSCs from porcine testes. METHODS:We confirmed th...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-014-0271-7

    authors： Park MH,Park JE,Kim MS,Lee KY,Park HJ,Yun JI,Choi JH,Lee Es,Lee ST

    更新日期：2014-08-01 00:00:00

  • Texture profile analysis reveals a stiffer ovarian cortex after testosterone therapy: a pilot study.

    abstract：PURPOSE:The importance of the surrounding ovarian stromal cells and extracellular matrix in the development and maturation of follicles has recently gained attention. An aberrant extracellular matrix has been described in ovaries of patients with polycystic ovary syndrome where a more rigid structural environment, poss...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-019-01513-x

    authors： De Roo C,Tilleman K,Vercruysse C,Declercq H,T'Sjoen G,Weyers S,De Sutter P

    更新日期：2019-09-01 00:00:00

  • An integrated investigation of oocyte developmental competence: expression of key genes in human cumulus cells, morphokinetics of early divisions, blastulation, and euploidy.

    abstract：PURPOSE:To investigate the association of cumulus cell (CC)-related expression of a selected cluster of key genes (PTGS2, CAMK1D, HAS2, STC1, and EFNB2) with embryo development to blastocyst. METHODS:Exploratory study at a private clinic. Eighteen advanced maternal age patients were enrolled (37.3 ± 4.0 years). Sevent...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-019-01410-3

    authors： Scarica C,Cimadomo D,Dovere L,Giancani A,Stoppa M,Capalbo A,Ubaldi FM,Rienzi L,Canipari R

    更新日期：2019-05-01 00:00:00

  • Live birth rates using conventional in vitro fertilization compared to intracytoplasmic sperm injection in Bologna poor responders with a single oocyte retrieved.

    abstract：PURPOSE:To compare reproductive outcomes following conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in poor responders fulfilling the Bologna criteria, with a single oocyte retrieved. METHODS:The present retrospective study included 243 Bologna poor responders with a single oocyte ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-015-0459-5

    authors： Sfontouris IA,Kolibianakis EM,Lainas GT,Navaratnarajah R,Tarlatzis BC,Lainas TG

    更新日期：2015-05-01 00:00:00

  • Vitamin D and assisted reproduction: should vitamin D be routinely screened and repleted prior to ART? A systematic review.

    abstract：PURPOSE:To review the current literature regarding the role of vitamin D status in pregnancy outcomes in women undergoing assisted reproductive technology (ART) and to assess cost-effectiveness of routine vitamin D deficiency screening and repletion prior to initiation of ART. METHODS:A systematic literature review wa...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s10815-014-0407-9

    authors： Pacis MM,Fortin CN,Zarek SM,Mumford SL,Segars JH

    更新日期：2015-03-01 00:00:00

  • Azoospermia and embryo morphokinetics: testicular sperm-derived embryos exhibit delays in early cell cycle events and increased arrest prior to compaction.

    abstract：PURPOSE:Sperm play an essential role in embryonic genome activation and embryonic progression to blastocyst. In the present work, we focus on development of embryos created as a result of ICSI with testicular or epididymal sperm from azoospermic males and compare this to outcomes from normospermic males. The objective ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-018-1183-8

    authors： Desai N,Gill P,Tadros NN,Goldberg JM,Sabanegh E,Falcone T

    更新日期：2018-07-01 00:00:00

  • Relative expression of genes encoding SMAD signal transduction factors in human granulosa cells is correlated with oocyte quality.

    abstract：PURPOSE:To determine the expression of SMAD transcripts in human granulosa cells. METHODS:Luteinized mural granulosa cells were harvested from forty women undergoing oocyte retrieval, and RNAs were isolated. SMAD expression levels were determined by polymerase chain reaction (PCR) and quantitative real-time PCR (q-RTP...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-011-9609-6

    authors： Kuo FT,Fan K,Ambartsumyan G,Menon P,Ketefian A,Bentsi-Barnes IK,Pisarska MD

    更新日期：2011-09-01 00:00:00

  • Autologous endometrial coculture in patients with a previous history of poor quality embryos.

    abstract：PURPOSE:To evaluate the effect of autologous endometrial coculture in patients (less than 36 years old) with a history of a single IVF failed cycle associated with poor quality embryos. METHODS: DESIGN:Controlled clinical study. SETTING:University-based in vitro fertilization center. PATIENTS:Twenty-six patients wi...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1023/a:1016062508127

    authors： Spandorfer SD,Barmat L,Navarro J,Burmeister L,Veeck L,Clarke R,Liu HC,Rosenwaks Z

    更新日期：2002-07-01 00:00:00

  • Investigation of effects of pregnant mare serum gonadotropin (PMSG) on the chromosomal complement of CD-1 mouse embryos.

    abstract：PURPOSE:The objective of this study was to examine the effect of superovulatory doses of gonadotropins on the frequency of chromosomal abnormalities of mouse embryos. METHODS:Chromosome analysis of 8- to 16-cell stage mouse embryos and zygotes was performed by a cytogenetic method. RESULTS:There was no significant ef...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02766134

    authors： Ma S,Kalousek DK,Yuen BH,Moon YS

    更新日期：1997-03-01 00:00:00

  • Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS.

    abstract：PURPOSE:The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage. METHODS:Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-017-0937-z

    authors： Mateo S,Vidal F,Parriego M,Rodríguez I,Montalvo V,Veiga A,Boada M

    更新日期：2017-07-01 00:00:00

  • What is the contribution of embryo-endometrial asynchrony to implantation failure?

    abstract：PURPOSE:The synchronized development of a viable embryo and a receptive endometrium is critical for successful implantation to take place. The aim of this paper is to review current thinking about the importance of embryo-endometrial synchrony in in vitro fertilization (IVF). METHODS:Detailed review of the literature ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s10815-016-0773-6

    authors： Teh WT,McBain J,Rogers P

    更新日期：2016-11-01 00:00:00

  • Successful pregnancy after the vitrification of zygotes using commercial vitrification solutions and conventional straws to protect against infections in liquid nitrogen.

    abstract：PURPOSE:To report on successful birth after the transfer of postthawed human zygotes that were vitrified using a conventional straw for the purpose of protecting them from infections and a low-toxicity cryoprotectant that is commercially sold. METHODS:A primary infertile couple presented at our IVF program. After bein...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-005-0818-8

    authors： Kumasako Y,Kumon M,Utsunomiya T,Araki Y

    更新日期：2005-01-01 00:00:00

  • Frozen-thawed embryo transfer cycles: clinical outcomes of single and double blastocyst transfers.

    abstract：PURPOSE:To evaluate clinical outcomes of frozen-thawed embryo transfer cycles when one or two blastocysts are transferred. METHODS:Retrospective chart review RESULTS:Two hundred forty-three frozen blastocyst transfer (FBT) cycles were analyzed. Clinical pregnancy rate (50.4% vs. 34.7%), live birth rate (45.8% vs. 30....

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-011-9551-7

    authors： Berin I,McLellan ST,Macklin EA,Toth TL,Wright DL

    更新日期：2011-07-01 00:00:00

  • The influence of uterine position on pregnancy rates with in vitro fertilization-embryo transfer.

    abstract：:We found no difference in clinical pregnancy rates following IVF-ET in women with anteverted versus retroverted uteri. Patients can be reassured that the probability of clinical pregnancy following IVF-ET is not compromised by uterine position. ...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/BF02215722

    authors： Agarwal SK,Coe S,Buyalos RP

    更新日期：1994-07-01 00:00:00

  • Chromosomal abnormalities in couples with recurrent spontaneous miscarriage: a 21-year retrospective study, a report of a novel insertion, and a literature review.

    abstract：PURPOSE:The aim of this study is to evaluate the frequency and nature of chromosomal abnormalities in Moroccan couples with recurrent spontaneous miscarriage (RSM). In addition, the data were compared with those reported elsewhere in order to give a global estimation of chromosomal abnormalities frequencies. METHODS:T...

    journal_title：Journal of assisted reproduction and genetics

    pub_type： 杂志文章

    doi：10.1007/s10815-018-1373-4

    authors： Elkarhat Z,Kindil Z,Zarouf L,Razoki L,Aboulfaraj J,Elbakay C,Nassereddine S,Nasser B,Barakat A,Rouba H

    更新日期：2019-03-01 00:00:00