Abstract：
OBJECTIVE:The exact chemical composition of hydrosalpingeal fluid is unknown. The objective of this study was to characterize cytokines in hydrosalpingeal fluid (HSF) and examine their possible role in the embryo development. STUDY DESIGN:HSF was aspirated at laparoscopic salpingectomy in eight infertile women. Levels of IL-1beta, IL-13, IL-8, IL-6 and TNF-alpha in the HSF were determined by quantitative immunoassay kits. Two-cell mouse embryos were incubated with 0, 25, 50 and 75% concentrations of HSF. The blastocyst development rate (BDR) of mouse embryos was measured at each HSF concentration. RESULT(S):The embryotoxicity of HSF was concentration dependent. An increase in the HSF concentration resulted in significant decrease in % BDR (p < 0.01). IL-1beta was present in six of the eight HSF samples with a mean (+/-SD) concentration of 0.9 +/- 0.8 pg/mL. IL-13 was not detected in any of the HSF samples. IL-8, IL-6 and TNF-alpha were detected in all samples with a mean (+/-SD) concentration of 4741.2 +/- 6554.4 pg/mL, 204.8+/-132.8 pg/ml and 12 +/- 12.8 pg/mL respectively. IL-6 was positively correlated with BDR (r = 0.53; p < 0.04). CONCLUSION(S):We demonstrated for the first time the presence of IL-1beta, IL-8, IL-6 and TNF-alpha and the absence of IL-13 in human hydrosalpingeal fluid. IL-6 was positively related to the BDR.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Bedaiwy MA,Falcone T,Goldberg JM,Attaran M,Sharma R,Miller K,Nelson DR,Agarwal Adoi
10.1007/s10815-005-4913-7keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
161-5issue
4eissn
1058-0468issn
1573-7330journal_volume
22pub_type
杂志文章abstract：PURPOSE:To perform complex preimplantation genetic tests (PGT) for aneuploidy screening, Robertsonian translocation, HLA-matching, and X-linked hyper IgM syndrome (XHIGM) caused by a novel mutation c.156 G>T of CD40LG gene. METHODS:Reverse transcription PCR (RT-PCR) and Sanger sequencing were carried out to confirm th...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01846-y
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:To study the effects of serum and growth factors on propagation of porcine male germline stem cells (MGSCs) in vitro and develop a culture system for these stem cells. METHODS:Fresh testicular cells from neonatal piglets were obtained by mechanical dissociation and collagenase-trypsin digestion. After differen...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0031-0
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:To compare pregnancy, implantation, and multiple gestation rates resulting from day 3 and day 5 embryo transfers after in vitro fertilization emphasizing a subset of patients who met criteria for day 5 transfer but elected to undergo day 3 transfer. METHOD:A retrospective analysis of day 3 and day 5 embryo tra...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1026240827476
更新日期：2003-10-01 00:00:00
abstract：PURPOSE:To investigate the influence of sperm DNA integrity on the zona binding ability of mouse spermatozoa in relation to their sex chromosomal constitution. METHOD(S):In this prospective experimental study, the sperm DNA fragmentation was induced by exposing testicular area of Swiss Albino mice (Mus musculus) to di...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0123-x
更新日期：2013-12-01 00:00:00
abstract：PURPOSE:To characterize each chromosome's risk for being involved in embryonic aneuploidy. METHODS:This is a retrospective cohort study conducted at a single, academic center. The cohort consisted of 15,169 consecutive trophectoderm biopsies which then underwent comprehensive chromosome screening utilizing validated r...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-014-0333-x
更新日期：2014-11-01 00:00:00
abstract：PURPOSE:The basic semen parameters seem to have a limited predictive value in male fertility. Could other objective sperm analyses be helpful in the choice of the most adapted assisted procreation technique? METHODS:This study concerns 78 infertile couples with insemination failures. For each semen, 21 objective param...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02765835
更新日期：1997-07-01 00:00:00
abstract：PURPOSE:To compare basal and clomiphene citrate (CC) induced follicle-stimulating hormone (FSH), estradiol (E2), and inhibin B levels with ultrasound indices of ovarian reserve in infertile women and to test the prognostic value of these tests on response to ovarian stimulation in in vitro fertilization (IVF). METHODS...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/b:jarg.0000025936.73125.b4
更新日期：2004-02-01 00:00:00
abstract：:Gender selection by PGD is an appropriate use of medical resources. Children borne through PGD for gender determination would be welcome and would come into a couple's life at a planned, opportune time. If the practice were made more available through insurance coverage, the size and makeup of families could become a ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1016871925450
更新日期：2002-09-01 00:00:00
abstract：PURPOSE:The objective of this study was to examine the effect of superovulatory doses of gonadotropins on the frequency of chromosomal abnormalities of mouse embryos. METHODS:Chromosome analysis of 8- to 16-cell stage mouse embryos and zygotes was performed by a cytogenetic method. RESULTS:There was no significant ef...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02766134
更新日期：1997-03-01 00:00:00
abstract：PROBLEM:Maternal stress perception is frequently alleged as a cause of infertility, miscarriages, late pregnancy complications or impaired fetal development. The purpose of the present review is to critically assess the biological and epidemiological evidence that considers the plausibility of a stress link to human re...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,评审
doi：10.1007/s10815-008-9206-5
更新日期：2008-02-01 00:00:00
abstract：PURPOSE:A higher proportion of male offspring has been observed after transferring faster-developing embryos in a number of animal species. Therefore, we evaluated the correlation between the sex ratio of delivered babies and the cleavage stage of transferred embryos in a human IVF-ET program. METHODS:The sex of infan...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02212575
更新日期：1995-10-01 00:00:00
abstract：PURPOSE:To investigate whether unexplained infertility at a young age demonstrates manifestations of decreased ovarian reserve. METHODS:A total of 100 women were divided into two equally sized groups. The study group comprised women aged ≤ 37 years diagnosed with unexplained infertility, and the control group included...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-019-01467-0
更新日期：2019-06-01 00:00:00
abstract：PURPOSE:To test the effects of type of embryo transfer catheter, transfer difficulty, and observations after the transfer procedure on pregnancy and implantation rates in an IVF programme. METHODS:Patients were prepared for IVF using standard protocols. Embryo transfer was performed using either Edwards-Wallace or TDT...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1014054421789
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:Obesity is associated with several fertility disorders. This prospective cohort study was designed to evaluate the effect of body mass index (BMI) (kg/m2) on oocyte diameter and treatment. METHODS:Women undergoing in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI) were enrolled in the...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-017-0975-6
更新日期：2017-09-01 00:00:00
abstract：PURPOSE:The purpose of this study is to determine if baseline antral follicle assessment may serve as additional information in predicting in vitro fertilization outcome. METHODS:Prospective, descriptive preliminary study of in vitro fertilization outcome. From July 1998 to July 1999, 224 patients underwent antral fol...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1009424407082
更新日期：2001-03-01 00:00:00
abstract：OBJECTIVE:Evaluate sperm chromatin stability and its relationship with the rate of fertilization after procedures of intracytoplasmic sperm injection (ICSI) in a program of assisted reproduction. DESIGN:Prospective study. SETTING:Institute of Gynecology and Reproduction. PATIENTS:Thirty-three women with their respec...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-007-9174-1
更新日期：2007-12-01 00:00:00
abstract：PURPOSE:Our purpose was to compare the diagnostic efficacy of laparoscopy between primary and secondary infertility and to define a subgroup in which the positive findings are low. METHODS:A retrospective review of the cases of 206 infertile women who underwent laparoscopy was conducted. RESULTS:Eighty-two (39.8%) pa...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1022578019260
更新日期：1998-10-01 00:00:00
abstract：PURPOSE:To review the history of experimental embryo culture and how culture media that permitted complete preimplantation development in vitro were first discovered, and the physiological insights gained. METHODS:This article reviews the history of in vitro mammalian embryo culture, in particular the efforts that led...
journal_title：Journal of assisted reproduction and genetics
pub_type： 历史文章,杂志文章,评审
doi：10.1007/s10815-013-0095-x
更新日期：2013-08-01 00:00:00
abstract：PURPOSE:We investigated if automated TLI selection may be a valuable strategy to identify those euploid embryos with the best chances of success. METHODS:This is a unicentric and retrospective study involving 244 patients undergoing preimplantational genetic screening (PGS) cycles with autologous oocytes or oocyte don...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-018-1265-7
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND:Literature supports the efficacy of psychosocial intervention for infertile women. An eastern body-mind-spirit group intervention has been developed to help infertile women in the Chinese population cope with the distress arising from IVF treatment. METHODS:The eastern body-mind-spirit group intervention ad...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-005-6418-9
更新日期：2005-12-01 00:00:00
abstract：PURPOSE:Revise role of hormonal basal and dynamic tests, as well as ultrasonographic measures as ovarian reserve markers, in order to provide better counseling to subfertile couples. METHODS:Review of publications on the topic, with an emphasis on recent well designed articles. RESULTS:Currently available ovarian res...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,评审
doi：10.1007/s10815-008-9241-2
更新日期：2008-07-01 00:00:00
abstract：PURPOSE:To use conflict resolution analysis on the conflict between proponents and opponents of preimplantation genetic testing for aneuploidy (PGT-A), previously called preimplantation genetic screening (PGS). METHODS:Considered in conflict analysis a case study, we reviewed the English literature based on key-word s...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01688-8
更新日期：2020-03-01 00:00:00
abstract：PURPOSE:We studied the influence of aging, hyaluronidase removal of the cumulus, and microinjection on the sperm binding potential of human oocytes under intact zona assay conditions to determine the safe use of unstored aged unfertilized ICSI oocytes on zona binding tests. Results were also compared with those for age...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02765778
更新日期：1997-02-01 00:00:00
abstract：PURPOSE:To identify gender differences in leadership and academic rank within academic reproductive endocrinology (REI) programs with fellowships in the USA. METHODS:Official institutional websites of the 2017-2018 American Board of Obstetrics and Gynecology (ABOG)-accredited reproductive endocrinology fellowship prog...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01866-8
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. METHODS:A total of 160 women who underwent vitrified-warmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10815-013-9984-2
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To report cases of in vitro fertilization-frozen embryo transfer (IVF-FET) with single blastocyst transfer resulting in di- or tri-chorionic pregnancies, and to review the literature on monozygotic, multi-chorionic pregnancies originating at the blastocyst stage. DESIGN:Retrospective case series and literatu...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,评审
doi：10.1007/s10815-018-1329-8
更新日期：2018-12-01 00:00:00
abstract：PURPOSE:this study was conducted on the effects of vitrification cryotop method on gene expression of mature oocytes in Mus musculus. METHODS:transcript analyses of three mouse genes, namely Mater, Hook1 and Sod1, were performed upon non-vitrified and vitrified oocytes with different concentrations of dimethyl sulfoxi...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-010-9453-0
更新日期：2010-11-01 00:00:00
abstract：PURPOSE:A pilot study was performed to test the diagnostic value of in vitro DNA fluorescence in oocytes that failed to fertilize after IVF. Ten patients with a cleavage rate less than 20% after IVF were included. RESULTS:Uncleaved oocytes were observed by fluorescence microscopy after incubation with the DNA fluoresc...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF01204255
更新日期：1992-12-01 00:00:00
abstract：PURPOSE:Our purpose was to determine if pregnancy rates (PRs) for hMG (human menopausal gonadotropin)-stimulated IVF-ET (in vitro fertilization--embryo transfer) can be increased by estradiol (E2) supplementation from the early proliferative phase to the late secretory phase of the endometrium. METHOD:Eighty-one infer...
journal_title：Journal of assisted reproduction and genetics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1023/a:1009445913156
更新日期：2000-01-01 00:00:00
abstract：PURPOSE:This study measured serum early pregnancy factor (EPF) in pregnant red deer (Cervus elaphus) and ascertained whether EPF synthesis is associated with implantation. METHODS:Serial serum samples were taken from mated hinds up to 42 days postconception and analyzed for EPF activity using the rosette inhibition te...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02765751
更新日期：1997-01-01 00:00:00
