当前位置： SCI文献检索 > JOURNAL OF ASSISTED REPRODUCTION AND GENETICS期刊下所有文献 > Sperm chromatin stability and its relationship with fertilization rate after intracytoplasmic sperm injection (ICSI) in an assisted reproduction program.

Sperm chromatin stability and its relationship with fertilization rate after intracytoplasmic sperm injection (ICSI) in an assisted reproduction program.

Abstract：

OBJECTIVE:Evaluate sperm chromatin stability and its relationship with the rate of fertilization after procedures of intracytoplasmic sperm injection (ICSI) in a program of assisted reproduction. DESIGN:Prospective study. SETTING:Institute of Gynecology and Reproduction. PATIENTS:Thirty-three women with their respective partners (12 couples in the study group and 21 couples in the control group) participating in a program of assisted reproduction. The study group was defined as men with >30% of non-decondensed spermatozoa (high sperm chromatin stability). INTERVENTIONS:A part of each seminal sample was used to evaluate sperm chromatin stability under SDS and EDTA treatment and the second aliquot was used for the ICSI procedure. Fertilization was evaluated 16-18 h post sperm injection at a pronuclear stage. The fertilized oocytes were further cultured for 24-48 h before transfer to the patient. MAIN OUTCOME MEASURES:Fertilization rate. RESULTS:Thirty-five oocytes (35.7%) in the group of study and 109 oocytes (78.9%) in the control group showed two pronuclei (P < 0.001). The coefficient of determination between the SDS + EDTA (Grade 2) and rate of fertilization was r (2) = 0.85 (P < 0.001) and the coefficient of regression was 1.72 +/- 0.19 (beta +/- ES) (P < 0.001). CONCLUSIONS:High sperm chromatin stability is a factor which reduces the rate of fertilization after ICSI procedure.

journal_name

J Assist Reprod Genet

journal_title

Journal of assisted reproduction and genetics

authors

García J,Noriega-Hoces L,Gonzales GF

doi

10.1007/s10815-007-9174-1

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

587-93

issue

12

eissn

1058-0468

issn

1573-7330

journal_volume

24

pub_type

杂志文章

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