Abstract:
PURPOSE:Oocyte in vitro maturation (IVM) is a patient-friendly reproductive technology but lower success rates than IVF have limited its uptake. Capacitation-IVM (CAPA-IVM) is an innovative new IVM system currently undergoing clinical evaluation. This study aimed to determine temporal effects of the pre-IVM phase of CAPA-IVM on cumulus function and oocyte developmental competence in mildly-stimulated mice. METHODS:Immature cumulus oocyte complexes (COCs) derived from mildly stimulated (23 h PMSG) 28-day-old mice underwent pre-IVM for 0-24 h in medium containing c-type natriuretic peptide (CNP), E2, FSH and insulin, prior to IVM (CAPA-IVM). The effect of pre-IVM duration on cumulus cell function and embryo development post-CAPA-IVM/IVF was assessed. RESULTS:Day 6 blastocyst rate increased incrementally with increasing pre-IVM duration: 40.6 ± 2.0%, 45.8 ± 1.2%, 52.2 ± 3.5%, 53.3 ± 5.9%, and 59.9 ± 2.5% for 0, 2, 6, 12, and 24 h pre-IVM, respectively (P < 0.01). DNA content/COC, a measure of cumulus cell proliferation, was significantly higher with 24 h pre-IVM group compared to 0, 2, or 6 h pre-IVM (P < 0.001). Pre-IVM for 24 h significantly increased cumulus expansion and mRNA expression of matrix genes Has2 and Tnfaip6 and Areg relative to no pre-IVM control (P < 0.01). Cumulus-oocyte gap-junctional communication (GJC) was maintained throughout 24 h pre-IVM (P < 0.0001), and GJC loss was slowed during the subsequent IVM phase, whilst meiotic resumption was accelerated (P < 0.05). Pre-IVM increased COC ATP and ADP content (P < 0.05), but not AMP, ATP/ADP, and energy charge. CONCLUSION:The pre-IVM phase of CAPA-IVM improves the quality of IVM oocytes in a temporally dependent manner and significantly influences cumulus cell function including increased cell proliferation, cumulus expansion, and prolonged cumulus-oocyte GJC.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Zhao Y,Liao X,Krysta AE,Bertoldo MJ,Richani D,Gilchrist RBdoi
10.1007/s10815-019-01610-xsubject
Has Abstractpub_date
2020-01-01 00:00:00pages
77-88issue
1eissn
1058-0468issn
1573-7330pii
10.1007/s10815-019-01610-xjournal_volume
37pub_type
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journal_title:Journal of assisted reproduction and genetics
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更新日期:2018-07-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
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abstract:PURPOSE:The purpose of this study was to examine the efficacy of an ovarian tissue transportation network for fertility preservation (FP) for cancer patients in Japan. METHODS:PubMed was searched for papers on transportation of human ovarian tissue for FP. We analyzed population, area, number of cancer patients for ov...
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doi:10.1007/s10815-017-1022-3
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,评审
doi:10.1007/s10815-018-1329-8
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journal_title:Journal of assisted reproduction and genetics
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,评审
doi:10.1007/s10815-020-01924-1
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journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章
doi:10.1007/BF02213692
更新日期:1994-01-01 00:00:00
abstract:PURPOSE:To assess the effect of wearing powdered gloves during embryo transfer as compared to un-powdered gloves on the pregnancy outcome of IVF cycles. METHODS:Patients, undergoing embryo transfer procedures, were prospectively randomized into two groups: In the first (group A, n=356) group embryo transfer was perfor...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-008-9285-3
更新日期:2009-01-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,评审
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更新日期:2020-08-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-015-0616-x
更新日期:2016-01-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/BF02066532
更新日期:1996-07-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1993-05-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,随机对照试验
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更新日期:2006-05-01 00:00:00
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更新日期:2012-07-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章
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更新日期:2016-03-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,meta分析
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更新日期:2013-12-01 00:00:00
abstract:PURPOSE:To assess the efficacy of a protocol involving the discontinuation of the GnRH analogue at the mid-phase of ovarian stimulation for IVF in patients with a previous poor response. METHODS:Prospective case-control evaluation compared with same patient's previous performance. Thirty-six patients enrolled in an IV...
journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章
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更新日期:2001-04-01 00:00:00