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Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures.

Abstract：

PURPOSE:We investigated whether enzymatically fabricated hyaluronan (HA) microcapsules were feasible for use in the cryopreservation of a small number of sperm. METHODS:HA microcapsules were fabricated using a system of water-immiscible fluid under laminar flow. Three sperm were injected into a hollow HA microcapsule using a micromanipulator. Capsules containing injected sperm were incubated in a freezing medium composed of sucrose as the cryoprotectant and then placed in a Cryotop® device and plunged into liquid nitrogen. After thawing, the capsule was degraded by hyaluronidase, and the recovery rate of sperm and their motility were investigated. RESULTS:The HA microcapsule measuring 200 μm in diameter and with a 30-μm thick membrane was handled using a conventional intracytoplasmic sperm injection (ICSI) system, and the procedure involved the injection of sperm into the capsule. The HA microcapsules containing sperm were cryopreserved in a Cryotop® device and decomposed by the addition of hyaluronidase. The recovery rate of sperm after cryopreservation and degradation of HA microcapsules was sufficient for use in clinical practice (90 %). CONCLUSIONS:Hollow HA microcapsules can be used for the cryopreservation of a small number of sperm without producing adverse effects on sperm quality.

journal_name

J Assist Reprod Genet

journal_title

Journal of assisted reproduction and genetics

authors

Tomita K,Sakai S,Khanmohammadi M,Yamochi T,Hashimoto S,Anzai M,Morimoto Y,Taya M,Hosoi Y

doi

10.1007/s10815-016-0656-x

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

501-11

issue

4

eissn

1058-0468

issn

1573-7330

pii

10.1007/s10815-016-0656-x

journal_volume

33

pub_type

杂志文章

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