Abstract：
PURPOSE:To ascertain possible cell damage from cryopreservation, the ultrastructure of human oocytes cryopreserved by slow cooling was assessed. MATERIALS AND METHODS:Cryopreservation was performed through two protocols with one-step or two-step propanediol. Fresh control oocytes were examined for comparison. Samples were processed for transmission electron microscopy analysis. RESULTS:By light microscopy, both fresh and frozen-thawed oocytes appeared regularly rounded, with intact zona pellucida, and homogeneous cytoplasm. By electron microscopy observation, organelles were abundant and uniformly dispersed. Mitochondria-smooth endoplasmic reticulum associations appeared regular. However, both the amount and density of cortical granules appeared abnormally reduced in frozen-thawed samples. Slight to moderate vacuolization was also found in the ooplasm of oocytes of both frozen groups. CONCLUSIONS:Slow cooling ensures a good overall preservation of human oocytes. However, cytoplasmic vacuolization and cortical granule loss appears associated with cryopreservation, irrespective of the protocol used.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Coticchio G,Borini A,Distratis V,Maione M,Scaravelli G,Bianchi V,Macchiarelli G,Nottola SAdoi
10.1007/s10815-010-9394-7subject
Has Abstractpub_date
2010-04-01 00:00:00pages
131-40issue
4eissn
1058-0468issn
1573-7330journal_volume
27pub_type
杂志文章abstract：PURPOSE:To identify gender differences in leadership and academic rank within academic reproductive endocrinology (REI) programs with fellowships in the USA. METHODS:Official institutional websites of the 2017-2018 American Board of Obstetrics and Gynecology (ABOG)-accredited reproductive endocrinology fellowship prog...
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journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
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更新日期：2018-03-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
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更新日期：2012-11-01 00:00:00
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更新日期：2020-03-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
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更新日期：2013-08-01 00:00:00
abstract：PURPOSE:Cumulus cells (CC) play important roles in oocyte development and cumulus expressed genes can be used as markers for oocyte quality. This study aimed to investigate temporal changes in the expression of cumulus marker genes during oocyte maturation as possible biomarkers of embryo developmental competence in ov...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-017-0998-z
更新日期：2017-11-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
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doi：10.1023/a:1009405128160
更新日期：2000-07-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
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journal_title：Journal of assisted reproduction and genetics
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更新日期：2006-07-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,随机对照试验
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更新日期：2006-05-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02766113
更新日期：1997-04-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
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更新日期：2019-05-01 00:00:00
abstract：PURPOSE:The basic semen parameters seem to have a limited predictive value in male fertility. Could other objective sperm analyses be helpful in the choice of the most adapted assisted procreation technique? METHODS:This study concerns 78 infertile couples with insemination failures. For each semen, 21 objective param...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02765835
更新日期：1997-07-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-009-9315-9
更新日期：2009-06-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0088-9
更新日期：2013-11-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
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更新日期：2010-09-01 00:00:00
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journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
