Epidermal growth factor (EGF) receptor localization in cultured human granulosa lutein cells and the stimulation of progesterone production by EGF and transforming growth factor-alpha (TGF-alpha).


PURPOSE:The purpose of this study was to investigate the expression of EGF receptor (EGF-R) in human granulosa cells undergoing luteinization and progesterone production by these cells in response to EGF an TGF-alpha alone or in combination with luteinizing hormone (LH). Granulosa cells were obtained from IF patients following oocyte retrieval 34 to 36 hr post-hCG injection. EGF receptor was localized in cells by means of immunoperoxidase staining using a polyclonal primary antibody directed against the human EGF-R. To assess progesterone production, cells were seeded overnight, washed, and cultured with the growth factors +/- LH. Medium and treatments were changed every 24 hr for 3 days. RESULTS:Specific EGF-R staining was observed in the cultured cells compared to those incubated with antibody that was preabsorbed with a 10-fold excess of EGF. Basal progesterone accumulation per 24-hr period was stimulated dose dependently on each day of culture, by both EGF (up to 3.5-fold at 5 or 50 ng/ml) and TGF-alpha (up to 4-fold at 50 ng/ml). The addition of LH alone also stimulated progesterone accumulation daily, and this effect was further enhanced dose dependently by cotreatment with EGF or TGF-alpha. Furthermore, tyrphostin, an EGF-R-specific tyrosine kinase inhibitor, inhibited both basal and growth factor-stimulated progesterone production. CONCLUSION:These data suggest an EGF receptor-mediated physiological role for EGF and TGF-alpha in human luteal function involving an autocrine and/or a paracrine stimulation of progesterone production.


J Assist Reprod Genet


Tekpetey FR,Daniel SA,Yuzpe A




Has Abstract


1995-11-01 00:00:00












  • Morphokinetics of embryos developed from oocytes matured in vitro.

    abstract:PURPOSE:In in vitro maturation (IVM) cycles primed with human chorionic gonadotropin (hCG), both immature and mature oocytes are retrieved from antral follicles sized 8-12 mm. Using time-lapse microscopy, we compared the morphokinetic behavior of embryos developed from oocytes matured in vivo and in vitro, testing the ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Dal Canto M,Novara PV,Coticchio G,Mignini Renzini M,Brambillasca F,Brigante C,De Ponti E,Fadini R

    更新日期:2016-02-01 00:00:00

  • The effect of ease of transfer and type of catheter used on pregnancy and implantation rates in an IVF program.

    abstract:PURPOSE:To test the effects of type of embryo transfer catheter, transfer difficulty, and observations after the transfer procedure on pregnancy and implantation rates in an IVF programme. METHODS:Patients were prepared for IVF using standard protocols. Embryo transfer was performed using either Edwards-Wallace or TDT...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: De Placido G,Wilding M,Stina I,Mollo A,Alviggi E,Tolino A,Colacurci N,De ML,Marino M,Dale B

    更新日期:2002-01-01 00:00:00

  • A comparison of oocyte donors' and gestational carriers/surrogates' attitudes towards third party reproduction.

    abstract:PURPOSE:This study was designed to compare levels of satisfaction for ovum donors and gestational carriers/surrogates (GCS), investigate attitudes, and explore beliefs about the role genetics, gestation, and environment play in various characteristics. DESIGN:An east coast IVF center and two California-based agencies ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Bravennan AM,Corson SL

    更新日期:2002-10-01 00:00:00

  • Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage?

    abstract:PURPOSE:We investigated antioxidant effects of CoQ10 supplementation on the prevention of OS-induced ovarian damage and to evaluate the protective effect of such supplementation against OS-related DNA damage. METHODS:Twenty-four adult female Sprague-Dawley rats were randomly divided into three groups (8 rats per group...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Özcan P,Fıçıcıoğlu C,Kizilkale O,Yesiladali M,Tok OE,Ozkan F,Esrefoglu M

    更新日期:2016-09-01 00:00:00

  • A comparison of the Cook single lumen immature ovum IVM needle to the Steiner-Tan pseudo double lumen flushing needle for oocyte retrieval for IVM.

    abstract:PURPOSE:This study compared the impact of using the Steiner-Tan pseudo double lumen needle for antral follicle oocyte retrieval to using a conventional non-flushing needle. The Steiner-Tan needle has a much smaller dead space than the needles commonly used for IVM oocyte retrievals. METHODS:This was a retrospective co...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Rose BI,Laky D

    更新日期:2013-06-01 00:00:00

  • Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes.

    abstract:OBJECTIVE:The aim of this study was to assess the fertilizability of unfertilized aged human oocytes from failed in vitro fertilization (IVF) cycles using SUZI and ICSI. METHODS:A total of 363 oocytes which showed no fertilization after conventional IVF was subjected to assisted fertilization using SUZI or ICSI. The m...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章


    authors: Tsirigotis M,Bennett V,Nicholson N,Khalifa Y,Hogewind G,Yazdani N,Craft I

    更新日期:1994-09-01 00:00:00

  • IL-1β predicts IVF outcome: a prospective study.

    abstract:PURPOSE:Retrospective cohort studies have shown a relationship between maternal serum interleukin-1β (IL-1β) and interleukin-1 receptor antagonist (IL-1Ra) levels and in vitro fertilization (IVF) cycle outcome. The objective of this investigation was to explore the correlation between serum IL-1β and/or IL-1Ra levels o...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Kreines FM,Nasioudis D,Minis E,Irani M,Witkin SS,Spandorfer S

    更新日期:2018-11-01 00:00:00

  • Transcription profile of the insulin-like growth factor signaling pathway during human ovarian follicular development.

    abstract:PURPOSE:The IGF signaling cascade exerts important regulatory functions in human ovarian folliculogenesis. The scope of this study was to evaluate the transcription profile of insulin-like growth factor (IGF) genes during human ovarian follicle development and to analyze follicle fluid levels of key IGF proteins. METH...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Bøtkjær JA,Pors SE,Petersen TS,Kristensen SG,Jeppesen JV,Oxvig C,Andersen CY

    更新日期:2019-05-01 00:00:00

  • Improvement of IVF outcome in poor responders by discontinuation of GnRH analogue during the gonadotropin stimulation phase--a function of improved embryo quality.

    abstract:PURPOSE:To assess the efficacy of a protocol involving the discontinuation of the GnRH analogue at the mid-phase of ovarian stimulation for IVF in patients with a previous poor response. METHODS:Prospective case-control evaluation compared with same patient's previous performance. Thirty-six patients enrolled in an IV...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章


    authors: Schachter M,Friedler S,Raziel A,Strassburger D,Bern O,Ron-el R

    更新日期:2001-04-01 00:00:00

  • Is there any evidence for immunologically mediated or immunologically modifiable early pregnancy failure?

    abstract:PURPOSE:Human reproduction is an inefficient process. There is a high rate of loss of early pregnancies, often before the mother (or physician) knows she is pregnant. Genetic abnormalities can explain much of the wastage, but can it explain all of the failures? As embryos bear paternal and embryonic antigens foreign to...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,评审


    authors: Clark DA

    更新日期:2003-02-01 00:00:00

  • Pelvic abscess complicating an in vitro fertilization pregnancy and review of the literature.

    abstract:INTRODUCTION:In vitro fertilisation has become a very common procedure in the infertility practice due to both accessibility and good success rates. DISCUSSION:Complications, however, are constant reminders that no procedure is totally safe. In this paper, we present a case of IVF pregnancy that presented with pelvic ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Al-Kuran O,Beitawi S,Al-Mehaisen L

    更新日期:2008-07-01 00:00:00

  • G9a co-localized with histone H3 lysine 9 monomethylation but not dimethylation in a nuclear membrane-dependent manner during mouse preimplantation embryo development.

    abstract:PURPOSE:Histone H3 lysine 9 (H3K9) methylation plays an important role in the regulation of preimplantation embryo development. G9a has been reported to be a major H3K9mono (m1)/dimethylation(m2) methyltransferase and to contain nuclear localization signals. This study was performed to investigate the correlation betwe...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Li B,Tang N,Chen S,Li X,Huang X,Wang X,Sun F

    更新日期:2013-03-01 00:00:00

  • Association study between single-nucleotide variants rs12097821, rs2477686, and rs10842262 and idiopathic male infertility risk in Serbian population with meta-analysis.

    abstract:PURPOSE:A genome-wide association study conducted in the Han Chinese population identified three single nucleotide variants rs12097821, rs2477686, and rs10842262 as being significantly associated with non-obstructive azoospermia. Our aim was to evaluate the possible association between these susceptibility loci and idi...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Nemanja V,Zorana D,Nevena K,Suzana M,Ivan V,Branko B,Mirka D,Dusanka SP,Goran B

    更新日期:2020-11-01 00:00:00

  • Centrifugal pulsing increases the recovery of viable spermatozoa.

    abstract:PURPOSE:Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation. METHODS:A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Nu...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章


    authors: Bielfeld P,Acosta V,Jeyendran RS

    更新日期:1998-11-01 00:00:00

  • The effects of vitrification on gene expression in mature mouse oocytes by nested quantitative PCR.

    abstract:PURPOSE:this study was conducted on the effects of vitrification cryotop method on gene expression of mature oocytes in Mus musculus. METHODS:transcript analyses of three mouse genes, namely Mater, Hook1 and Sod1, were performed upon non-vitrified and vitrified oocytes with different concentrations of dimethyl sulfoxi...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Habibi A,Farrokhi N,Moreira da Silva F,Bettencourt BF,Bruges-Armas J,Amidi F,Hosseini A

    更新日期:2010-11-01 00:00:00

  • Sequential FISH allows the determination of the segregation outcome and the presence of numerical anomalies in spermatozoa from a t(1;8;2)(q42;p21;p15) carrier.

    abstract:PURPOSE:To find out the meiotic segregation behaviour of the t(1;8;2)(q42;p21;p15), to evaluate the occurrence of interchromosomal effects, and to determine whether there is an accumulation of unbalanced products in aneuploid/diploid gametes. METHODS:A sequential FISH protocol based on two successive hybridization rou...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Godo A,Blanco J,Vidal F,Parriego M,Boada M,Anton E

    更新日期:2013-09-01 00:00:00

  • Patient-centered elective egg freezing: a binational qualitative study of best practices for women's quality of care.

    abstract:PURPOSE:How can elective egg freezing (EEF) be made patient centered? This study asked women to reflect on their experiences of EEF, which included their insights and recommendations on the optimal delivery of patient-centered care. METHODS:In this binational, qualitative study, 150 women (114 in the USA, 36 in Israel...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Inhorn MC,Birenbaum-Carmeli D,Westphal LM,Doyle J,Gleicher N,Meirow D,Dirnfeld M,Seidman D,Kahane A,Patrizio P

    更新日期:2019-06-01 00:00:00

  • Ovarian stimulation and in-vitro fertilization outcomes of cancer patients undergoing fertility preservation compared to age matched controls: a 17-year experience.

    abstract:PURPOSE:To compare the in-vitro fertilization (IVF) outcomes of cancer patients who underwent oocyte retrieval and embryo/oocyte cryopreservation prior to gonadotoxic therapy to those of age and time-matched controls with tubal factor infertility. METHODS:All cancer patients who underwent embryo/oocyte cryopreservatio...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Cardozo ER,Thomson AP,Karmon AE,Dickinson KA,Wright DL,Sabatini ME

    更新日期:2015-04-01 00:00:00

  • Reference gene selection for real-time quantitative PCR analysis on ovarian cryopreservation by vitrification in mice.

    abstract:PURPOSE:To ensure the correct interpretation of the results of quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) from ovarian tissue cryopreserved by vitrification, it is critical to normalize expression levels to a reference gene with stable messenger RNA (mRNA) expression in the vitrifi...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Yuanyuan S,Qin S,Rongrong X,Yujing G,Chengbin P,Jianjun M,Yanzhou Y,Xiuying P

    更新日期:2015-08-01 00:00:00

  • Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis.

    abstract:PURPOSE:To study the effect of α-linolenic acid (ALA) on meiotic maturation, mRNA abundance of apoptosis-related (Bax and Bcl-2) molecules, and blastocyst formation in ovine oocytes. METHODS:A preliminary experiment was conducted to analyze the concentration of ALA in "small" (≤2 mm) and "large" (≥6 mm) follicles usin...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Veshkini A,Asadi H,Khadem AA,Mohammadi-Sangcheshmeh A,Khazabi S,Aminafshar M,Deldar H,Soleimani M,Cinar MU

    更新日期:2015-04-01 00:00:00

  • Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study.

    abstract:PURPOSE:To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. METHODS:A total of 160 women who underwent vitrified-warmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,随机对照试验


    authors: Ren X,Liu Q,Chen W,Zhu G,Zhang H

    更新日期:2013-06-01 00:00:00

  • The accumulation of vitrified oocytes is a strategy to increase the number of euploid available blastocysts for transfer after preimplantation genetic testing.

    abstract:PURPOSE:In a preimplantation genetic diagnosis for aneuploidy (PGD-A) program, the more embryos available for biopsy, consequently increases the chances of obtaining euploid embryos to transfer. The aim was to increase the number of viable euploid blastocysts in patients undergoing PGD-A using fresh oocytes together wi...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Chamayou S,Sicali M,Alecci C,Ragolia C,Liprino A,Nibali D,Storaci G,Cardea A,Guglielmino A

    更新日期:2017-04-01 00:00:00

  • Effect of inseminated volume on intrauterine insemination.

    abstract:PURPOSE:Intrauterine insemination (IUI) is a method for the treatment of marital infertility involving the intrauterine or fallopian deposition of washed spermatozoa, depending on the amount of inseminated semen. In view of the divergent opinions about the inseminated volume, the objective of this study was to compare ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章


    authors: Do Amaral VF,Ferriani RA,Dos Reis RM,De Sala MM,De Moura MD

    更新日期:2001-08-01 00:00:00

  • Can dissenting findings regarding the comparative effectiveness of ICSI and IVF be explained by a learning curve?

    abstract:PURPOSE:To determine whether between-trial heterogeneity in relative risk of fertilisation for intracytoplasmic sperm injection (ICSI) compared to in vitro fertilisation (IVF) can be explained by learning or by between-trial variation in patient characteristics. METHODS:Systematic review and meta-analysis of trials co...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,meta分析


    authors: Mortimer D,French S

    更新日期:2006-01-01 00:00:00

  • Sperm chromatin stability and its relationship with fertilization rate after intracytoplasmic sperm injection (ICSI) in an assisted reproduction program.

    abstract:OBJECTIVE:Evaluate sperm chromatin stability and its relationship with the rate of fertilization after procedures of intracytoplasmic sperm injection (ICSI) in a program of assisted reproduction. DESIGN:Prospective study. SETTING:Institute of Gynecology and Reproduction. PATIENTS:Thirty-three women with their respec...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: García J,Noriega-Hoces L,Gonzales GF

    更新日期:2007-12-01 00:00:00

  • Azoospermia and embryo morphokinetics: testicular sperm-derived embryos exhibit delays in early cell cycle events and increased arrest prior to compaction.

    abstract:PURPOSE:Sperm play an essential role in embryonic genome activation and embryonic progression to blastocyst. In the present work, we focus on development of embryos created as a result of ICSI with testicular or epididymal sperm from azoospermic males and compare this to outcomes from normospermic males. The objective ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Desai N,Gill P,Tadros NN,Goldberg JM,Sabanegh E,Falcone T

    更新日期:2018-07-01 00:00:00

  • Pronuclear and morphological features as a cumulative score to select embryos in ICSI (intracytoplasmic sperm injection) cycles according to sperm origin.

    abstract:PURPOSE:To analyze best parameter to select embryos according to sperm origin in ICSI cycles. METHODS:One hundred seventy-two ICSI cycles were divided among three different groups: A (ejaculated spermatozoa from nonmale factor infertility), B (ejaculated spermatozoa from oligospermia), and C (spermatozoa from azoosper...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Rossi-Ferragut LM,Iaconelli A Jr,Aoki T,Rocha CC,dos Santos DR,Pasqualotto FF,Borges E Jr

    更新日期:2003-01-01 00:00:00

  • Editing the human genome: where ART and science intersect.

    abstract::The rapid development of gene-editing technologies has led to an exponential rise in both basic and translational research initiatives studying molecular processes and investigating possible clinical applications. Early experiments using genome editing to study human embryo development have contradicted findings in st...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 社论


    authors: Hershlag A,Bristow SL

    更新日期:2018-08-01 00:00:00

  • Microinsemination techniques for the treatment of patients suffering agenesia of the vas deferens.

    abstract::Thirty cycles of microsurgical epididymal sperm aspiration were performed on 27 patients presenting agenesia of the vas deferens. Two techniques of microinsemination were used, depending on the quality of the sperm preparation: subzonal sperm microinjection and microdroplet insemination. The results obtained by both m...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Gianaroli L,Magli MC,Colpi G,Vitali G,Fortini D,Negri L,Ferraretti AP

    更新日期:1996-04-01 00:00:00

  • Angiotensin II (AII) modulation of steroidogenesis by luteinized granulosa cells in vitro.

    abstract:PURPOSE:The purpose of the present study was to evaluate the effect of angiotensin II and its inhibitor, saralasin, on steroid production by luteinized human granulosa cells in vitro. Granulosa cells were obtained from follicular fluid aspirations from human in vitro fertilization. Cultures were established in suppleme...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章


    authors: Morris RS,Francis MM,Do YS,Hsueh WA,Lobo RA,Paulson RJ

    更新日期:1994-03-01 00:00:00