Effect of Percoll wash on sperm motion parameters and subsequent fertility in intrauterine insemination cycles.

Abstract:

PURPOSE:This study examined sperm motion parameters as measured by computerized automated semen analysis before and after a Percoll wash and determined if differences in any parameter were correlated with fertility subsequent to intrauterine insemination (IUI). RESULTS:Total motile sperm decreased following the washing procedure from 79.0 +/- 9.0 to 37.2 +/- 7.6 million sperm. Motility increased from a mean of 43.4% to 61.7% (P < 0.001). Other motility parameters also changed significantly (P < 0.001) as follows: curvilinear velocity (VCL), 43.4 to 61.7 microns/s; straight-line velocity (VSL), 21.3 to 26.7 microns/s; linearity 53.1 to 45.2%; lateral head displacement (ALH), 2.97 to 3.94 microns. Similar changes occurred following a swim-up preparation, although changes in mean motility, VCL, and ALH were significantly greater when compared to Percoll. The postwash changes were not accounted for merely by time lapse in preparation since reanalyzed untreated controls did not show the same changes in motion parameters. Prewash linearity in those specimens which resulted in pregnancies was greater than in those which did not (P = 0.28). No other significant differences in pre- or post-Percoll washed sperm motion parameters were found between pregnant vs nonfertile cycles. CONCLUSION:Following Percoll wash all CASA-generated motility parameters were significantly altered, but there was little association between these parameters and pregnancy achieved in IUI cycles.

journal_name

J Assist Reprod Genet

authors

Swanson ML,Collins JM,Freiman SF,Dubin NH

doi

10.1007/BF02214129

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

48-54

issue

1

eissn

1058-0468

issn

1573-7330

journal_volume

12

pub_type

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