Investigations on DNA damage and frequency of micronuclei in occupational exposure to electromagnetic fields (EMFs) emitted from video display terminals (VDTs).

Abstract:

:The potential effect of electromagnetic fields (EMFs) emitted from video display terminals (VDTs) to elicit biological response is a major concern for the public. The software professionals are subjected to cumulative EMFs in their occupational environments. This study was undertaken to evaluate DNA damage and incidences of micronuclei in such professionals. To the best of our knowledge, the present study is the first attempt to carry out cytogenetic investigations on assessing bioeffects in personal computer users. The study subjects (n = 138) included software professionals using VDTs for more than 2 years with age, gender, socioeconomic status matched controls (n = 151). DNA damage and frequency of micronuclei were evaluated using alkaline comet assay and cytochalasin blocked micronucleus assay respectively. Overall DNA damage and incidence of micronuclei showed no significant differences between the exposed and control subjects. With exposure characteristics, such as total duration (years) and frequency of use (minutes/day) sub-groups were assessed for such parameters. Although cumulative frequency of use showed no significant changes in the DNA integrity of the classified sub-groups, the long-term users (> 10 years) showed higher induction of DNA damage and increased frequency of micronuclei and micro nucleated cells.

journal_name

Genet Mol Biol

authors

Lakshmi N,Tiwari R,Bhargava S,Ahuja Y

doi

10.1590/S1415-47572010005000010

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

154-8

issue

1

eissn

1415-4757

issn

1678-4685

journal_volume

33

pub_type

杂志文章
  • HLA-DRB1 alleles in four Amerindian populations from Argentina and Paraguay.

    abstract::The major histocompatibility complex (MHC) is one of the biological systems of major polymorphisms. The study of HLA class II variability has allowed the identification of several alleles that are characteristic to Amerindian populations, and it is an excellent tool to define the relations and biological affinities am...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009000200002

    authors: Parolín ML,Carnese FR

    更新日期:2009-04-01 00:00:00

  • Gender-dependent association of HSD11B1 single nucleotide polymorphisms with glucose and HDL-C levels.

    abstract::In this study, we investigated the influence of two SNPs (rs846910 and rs12086634) of the HSD11B1 gene that encodes 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), the enzyme that catalyzes the conversion of cortisol to cortisone, on variables associated with obesity and metabolic syndrome in 215 individuals of bot...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/s1415-47572014000400003

    authors: Turek LV,Leite N,Rodrigues Souza RL,Lima JK,Milano GE,Timossi Lda S,Osiecki AC,Osiecki R,Alle LF

    更新日期:2014-09-01 00:00:00

  • Xylella fastidiosa gene expression analysis by DNA microarrays.

    abstract::Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A t...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000038

    authors: Travensolo RF,Carareto-Alves LM,Costa MV,Lopes TJ,Carrilho E,Lemos EG

    更新日期:2009-04-01 00:00:00

  • Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genetic polymorphisms and atopic asthma in children from Southeastern Brazil.

    abstract::Xenobiotics can trigger degranulation of eosinophils and mast cells. In this process, the cells release several substances leading to bronchial hyperactivity, the main feature of atopic asthma (AA). GSTM1 and GSTT1 genes encode enzymes involved in the inactivation of these compounds. Both genes are polymorphic in huma...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572010000300007

    authors: Lima CS,Néri IA,Lourenço GJ,Faria IC,Ribeiro JD,Bertuzzo CS

    更新日期:2010-07-01 00:00:00

  • Identification of a novel mutation in ARSA gene in three patients of an Iranian family with metachromatic leukodystrophy disorder.

    abstract::Metachromatic leukodystrophy disorder (MLD) is an autosomal recessive and lysosomal storage disease. The disease is caused by the deficiency of the enzyme arylsulfatase A (ARSA) which is encoded by the ARSA gene. Different mutations have been reported in different populations. The present study was aimed to detect the...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2016-0110

    authors: Golchin N,Hajjari M,Malamiri RA,Aminzadeh M,Mohammadi-Asl J

    更新日期:2017-10-01 00:00:00

  • Chromosome identification in the Andean common bean accession G19833 (Phaseolus vulgaris L., Fabaceae).

    abstract::Characterization of all chromosomes of the Andean G19833 bean genotype was carried out by fluorescent in situ hybridization. Eleven single-copy genomic sequences, one for each chromosome, two BACs containing subtelomeric and pericentromeric repeats and the 5S and 45S ribosomal DNA (rDNA) were used as probes. Compariso...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572011005000029

    authors: Altrock S,Fonsêca A,Pedrosa-Harand A

    更新日期:2011-07-01 00:00:00

  • Successful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea.

    abstract::Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previous...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-475738320140376

    authors: Fávero AP,Dos Santos RF,Simpson CE,Valls JF,Vello NA

    更新日期:2015-07-01 00:00:00

  • The cytotoxic and growth inhibitory effects of palladium(II) complexes on MDA-MB-435 cells.

    abstract::The antitumorigenic potential of two palladium(II) complexes, [Pd(ca(2)-o-phen)Cl(2)] - C1 and [Pd(dmba)(dppp)Cl] - C2, was evaluated, using MDA-MB-435 cells, a human breast adenocarcinoma cell-line that does not express the estrogen receptor α (ER-). Growth inhibition and induced alterations in cell-morphology were a...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/s1415-47572012005000016

    authors: Campanella NC,da Silva Demartini M,Torres C,de Almeida ET,Gouvêa CM

    更新日期:2012-01-01 00:00:00

  • A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots.

    abstract::Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572012000200016

    authors: Rodrigues EP,Torres AR,da Silva Batista JS,Huergo L,Hungria M

    更新日期:2012-06-01 00:00:00

  • Aromatase gene and its effects on growth, reproductive and maternal ability traits in a multibreed sheep population from Brazil.

    abstract::We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000054

    authors: Lôbo AM,Lôbo RN,Paiva SR

    更新日期:2009-07-01 00:00:00

  • The PIN1 family gene PvPIN1 is involved in auxin-dependent root emergence and tillering in switchgrass.

    abstract::Switchgrass (Panicum virgatum L.; family Poaceae) is a warm-season C4 perennial grass. Tillering plays an important role in determining the morphology of aboveground parts and the final biomass yield of switchgrass. Auxin distribution in plants can affect a variety of important growth and developmental processes, incl...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2014-0300

    authors: Xu K,Sun F,Wang Y,Shi L,Liu S,Xi Y

    更新日期:2016-03-01 00:00:00

  • Comparative proteomic analysis of indica and japonica rice varieties.

    abstract::Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterize...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572014005000015

    authors: Yang Y,Zhu K,Xia H,Chen L,Chen K

    更新日期:2014-10-01 00:00:00

  • Plasma GBP2 promoter methylation is associated with advanced stages in breast cancer.

    abstract::Blood methylated cell-free DNA (cfDNA) as a minimally invasive cancer biomarker has great importance in cancer management. Guanylate binding protein 2 (GBP2) has been considered as a possible controlling factor in tumor development. GBP2 gene expression and its promoter methylation status in both plasma cfDNA and tumo...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2019-0230

    authors: Rahvar F,Salimi M,Mozdarani H

    更新日期:2020-11-17 00:00:00

  • A molecular method for the detection of sally lightfoot crab larvae (Grapsus grapsus, Brachyura, Grapsidae) in plankton samples.

    abstract::The decapod Grapsus grapsus is commonly found on oceanic islands of the Pacific and Atlantic coasts of the Americas. In this study, a simple, quick and reliable method for detecting its larvae in plankton samples is described, which makes it ideal for large-scale studies of larval dispersal patterns in the species. ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572011005000020

    authors: Ströher PR,Firkowski CR,Freire AS,Pie MR

    更新日期:2011-07-01 00:00:00

  • Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP.

    abstract::Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and D...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572012005000043

    authors: Raut AA,Kumar A,Kala SN,Chhokar V,Rana N,Beniwal V,Jaglan S,Samuchiwal SK,Singh JK,Mishra A

    更新日期:2012-07-01 00:00:00

  • Exploitation of mitochondrial nad6 as a complementary marker for studying population variability in Lepidoptera.

    abstract::The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers a...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572011000400028

    authors: Silva-Brandão KL,Lyra ML,Santos TV,Seraphim N,Albernaz KC,Pavinato VA,Martinelli S,Cônsoli FL,Omoto C

    更新日期:2011-10-01 00:00:00

  • DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser.

    abstract::This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2016-0297

    authors: Maia TA,Vilaça ST,Silva LRD,Santos FR,Dantas GPM

    更新日期:2017-10-01 00:00:00

  • Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata.

    abstract::Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:

    authors: Jamsari AF,Min-Pau T,Siti-Azizah MN

    更新日期:2011-04-01 00:00:00

  • Electroacupuncture inhibits apoptosis in annulus fibrosis cells through suppression of the mitochondria-dependent pathway in a rat model of cervical intervertebral disc degradation.

    abstract::The purpose of this study was to investigate whether treatment with electroacupuncture (EA) inhibited mitochondria-dependent apoptosis in annulus fibrosis (AF) cells in a rat model of cervical intervertebral disc degradation induced by unbalanced dynamic and static forces. Forty Sprague-Dawley rats were used in this s...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572012005000046

    authors: Liao J,Ke M,Xu T,Lin L

    更新日期:2012-07-01 00:00:00

  • Familial Dysautonomia: Mechanisms and Models.

    abstract::Hereditary Sensory and Autonomic Neuropathies (HSANs) compose a heterogeneous group of genetic disorders characterized by sensory and autonomic dysfunctions. Familial Dysautonomia (FD), also known as HSAN III, is an autosomal recessive disorder that affects 1/3,600 live births in the Ashkenazi Jewish population. The m...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2015-0335

    authors: Dietrich P,Dragatsis I

    更新日期:2016-10-01 00:00:00

  • Phylogenetic niche conservatism and plant diversification in South American subtropical grasslands along multiple climatic dimensions.

    abstract::Phylogenetic niche conservatism can be investigated at multiple scales on an explicit geographical context. Haplotype-based comparative analyses of lineages occupying the same region, and thus subjected to similar environmental factors, allow decoupling shared evolutionary and ecological patterns, as well as multiple ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2018-0291

    authors: Barros MJF,Silva-Arias GA,Segatto ALA,Reck-Kortmann M,Fregonezi JN,Diniz-Filho JAF,Freitas LB

    更新日期:2020-04-27 00:00:00

  • Birth defects in Brazil: Outcomes of a population-based study.

    abstract::Birth defects (BDs) are functional and structural alterations in embryonic or fetal development. With an incidence of approximately 3-5%, BDs are a leading cause of infant mortality and lifelong disability. A population-based prospective case-control study was conducted for one year with 5204 infants, between March 1s...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2018-0186

    authors: Oliveira-Brancati CIF,Ferrarese VCC,Costa AR,Fett-Conte AC

    更新日期:2020-02-10 00:00:00

  • bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells.

    abstract::In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce o...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/s1415-47572014000100019

    authors: Song X,Li Y,Chen X,Yin G,Huang Q,Chen Y,Xu G,Wang L

    更新日期:2014-03-01 00:00:00

  • DNA repair genes XRCC1 and XRCC3 polymorphisms and their relationship with the level of micronuclei in breast cancer patients.

    abstract::Breast cancer (BC) is the most prevalent type worldwide, besides being one of the most common causes of death among women. It has been suggested that sporadic BC is most likely caused by low-penetrance genes, including those involved in DNA repair mechanisms. Furthermore, the accumulation of DNA damage may contribute ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572010005000082

    authors: Santos RA,Teixeira AC,Mayorano MB,Carrara HH,Andrade JM,Takahashi CS

    更新日期:2010-10-01 00:00:00

  • Variation and genetic structure of Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations based on ISSR pattern.

    abstract::For a study of diversity and genetic structuring in Melipona quadrifasciata, 61 colonies were collected in eight locations in the state of Minas Gerais, Brazil. By means of PCR analysis, 119 ISSR bands were obtained, 80 (68%) being polymorphic. H(e) and H (B) were 0.20 and 0.16, respectively. Two large groups were obt...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572010005000052

    authors: Nascimento MA,Batalha-Filho H,Waldschmidt AM,Tavares MG,Campos LA,Salomão TM

    更新日期:2010-04-01 00:00:00

  • Clonal diversity and conservation genetics of the medicinal plant Carapichea ipecacuanha (Rubiaceae).

    abstract::The roots of the understorey shrub Carapichea ipecacuanha (ipecac) have medicinal properties, and the uprooting of wild plants has supplied most of the world demand for this species. Although under severe population decline, C. ipecacuanha lacks legal protection. In the wild, the aerial stems of ipecac clump together ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000096

    authors: de Oliveira LO,Venturini BA,Rossi AA,Hastenreiter SS

    更新日期:2010-01-01 00:00:00

  • Genetic evaluation of the mating system in the blue-and-yellow macaw (Ara ararauna, Aves, Psittacidae) by DNA fingerprinting.

    abstract::More than 90% of birds are socially monogamous, although genetic studies indicate that many are often not sexually monogamous. In the present study, DNA fingerprinting was used to estimate the genetic relationships between nestlings belonging to the same broods to evaluate the mating system in the socially monogamous ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572010005000112

    authors: Caparroz R,Miyaki CY,Baker AJ

    更新日期:2011-01-01 00:00:00

  • Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae).

    abstract::Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000040

    authors: da Silva GM,Bione EG,Cabral-de-Mello DC,de Moura Rde C,Simões ZL,de Souza MJ

    更新日期:2009-04-01 00:00:00

  • Cultivable bacteria isolated from apple trees cultivated under different crop systems: Diversity and antagonistic activity against Colletotrichum gloeosporioides.

    abstract::This study evaluated the diversity of cultivable plant growth-promoting (PGP) bacteria associated with apple trees cultivated under different crop management systems and their antagonistic ability against Colletotrichum gloeosporioides. Samples of roots and rhizospheric soil from apple trees cultivated in organic and ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/s1415-47572014000400013

    authors: Dos Passos JF,da Costa PB,Costa MD,Zaffari GR,Nava G,Boneti JI,de Oliveira AM,Passaglia LM

    更新日期:2014-09-01 00:00:00

  • ADP-ribosylation: from molecular mechanisms to human disease.

    abstract::Post-translational modification of proteins by ADP-ribosylation, catalysed by poly (ADP-ribose) polymerases (PARPs) using NAD+ as a substrate, plays central roles in DNA damage signalling and repair, modulates a range of cellular signalling cascades and initiates programmed cell death by parthanatos. Here, we present ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2019-0075

    authors: Hoch NC,Polo LM

    更新日期:2019-12-13 00:00:00