Abstract:
:Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy(p) type in D. nisus and D. semisquamosus and of the Xy (r) type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
da Silva GM,Bione EG,Cabral-de-Mello DC,de Moura Rde C,Simões ZL,de Souza MJdoi
10.1590/S1415-47572009005000040subject
Has Abstractpub_date
2009-04-01 00:00:00pages
276-80issue
2eissn
1415-4757issn
1678-4685journal_volume
32pub_type
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