Abstract:
:Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
Shahinnia F,Sayed-Tabatabaei BEdoi
10.1590/S1415-47572009005000047subject
Has Abstractpub_date
2009-07-01 00:00:00pages
564-7issue
3eissn
1415-4757issn
1678-4685journal_volume
32pub_type
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