Conversion of barley SNPs into PCR-based markers using dCAPS method.

Abstract:

:Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.

journal_name

Genet Mol Biol

authors

Shahinnia F,Sayed-Tabatabaei BE

doi

10.1590/S1415-47572009005000047

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

564-7

issue

3

eissn

1415-4757

issn

1678-4685

journal_volume

32

pub_type

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