Abstract:
:Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
Paiva MHS,Guedes DRD,Leal WS,Ayres CFJdoi
10.1590/1678-4685-GMB-2016-0312subject
Has Abstractpub_date
2017-07-01 00:00:00pages
597-599issue
3eissn
1415-4757issn
1678-4685pii
S1415-47572017005014104journal_volume
40pub_type
杂志文章abstract::In this study, we investigated the influence of two SNPs (rs846910 and rs12086634) of the HSD11B1 gene that encodes 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), the enzyme that catalyzes the conversion of cortisol to cortisone, on variables associated with obesity and metabolic syndrome in 215 individuals of bot...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000400003
更新日期:2014-09-01 00:00:00
abstract::Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue fr...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000300017
更新日期:2014-06-01 00:00:00
abstract::Sickle cell anemia is a genetic disease with high prevalence in people of African descent. There are five typical haplotypes associated with this disease and the haplotypes associated with the beta-globin gene cluster have been used to establish the origin of African-descendant people in America. In this work, we dete...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013000400005
更新日期:2013-12-01 00:00:00
abstract::Human longevity is a polygenic and multifactorial trait. Pathways related to lifespan are complex and involve molecular, cellular, and environmental processes. In this analytical observational study, we evaluated the relationship between environment factors, oxidative stress status, DNA integrity level, and the associ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2017-0169
更新日期:2018-04-01 00:00:00
abstract::Metachromatic leukodystrophy disorder (MLD) is an autosomal recessive and lysosomal storage disease. The disease is caused by the deficiency of the enzyme arylsulfatase A (ARSA) which is encoded by the ARSA gene. Different mutations have been reported in different populations. The present study was aimed to detect the...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0110
更新日期:2017-10-01 00:00:00
abstract::The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in Indi...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572011005000003
更新日期:2011-04-01 00:00:00
abstract::The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011000400028
更新日期:2011-10-01 00:00:00
abstract::Oryza sativa, the common cultivated rice, is one of the most important crops for human consumption, but production is increasingly threatened by abiotic stresses. Although many efforts have resulted in breeding rice cultivars that are relatively tolerant to their local environments, climate changes and population incr...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0093
更新日期:2017-01-01 00:00:00
abstract::Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012000200016
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abstract::Catalase (CAT) is a common antioxidant enzyme in almost all living organisms. Currently, detailed reports on cucumber (Cucumis sativus L.) CAT (CsCAT) genes and tissue expression profiling are limited. In the present study, four candidate CsCAT genes were identified in cucumber. Phylogenetic analysis indicated that Cs...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0192
更新日期:2016-07-01 00:00:00
abstract::Rhagomys rufescens (Rodentia: Sigmodontinae) is an endemic species of the Atlantic forest from Southern and Southeastern Brazil. Some authors consider Rhagomys as part of the tribe Thomasomyini; but its phylogenetic relationships remain unclear. Chromosomal studies on eight specimens of Rhagomys rufescens revealed a d...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000071
更新日期:2010-07-01 00:00:00
abstract::A 10-year-old speechless, mentally deficient male, with low arylsulfatase A (ARSA) activity, and presumably, methachromatic leukodystrophy, underwent genetic evaluation. As the clinical picture was not compatible with this diagnosisan ARSA gene and chromosome analysis were performed, showing the presence of a pseudode...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012000300007
更新日期:2012-04-01 00:00:00
abstract::In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572012005000013
更新日期:2012-01-01 00:00:00
abstract::More than 90% of birds are socially monogamous, although genetic studies indicate that many are often not sexually monogamous. In the present study, DNA fingerprinting was used to estimate the genetic relationships between nestlings belonging to the same broods to evaluate the mating system in the socially monogamous ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000112
更新日期:2011-01-01 00:00:00
abstract::The genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms respons...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000031
更新日期:2013-09-01 00:00:00
abstract::Exercise is a double-edged sword: when practiced in moderation, it increases the expression of antioxidant enzymes, but when practiced strenuously it causes oxidative stress and cell damage. In this context, polymorphisms in the interleukin (IL)-6 gene should be investigated better because they can influence performan...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0299
更新日期:2016-10-01 00:00:00
abstract::Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previous...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738320140376
更新日期:2015-07-01 00:00:00
abstract::Several phylogeographic studies of seabirds have documented low genetic diversity that has been attributed to bottleneck events or individual capacity for dispersal. Few studies have been done in seabirds on the Brazilian coast and all have shown low genetic differentiation on a wide geographic scale. The Kelp Gull is...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000065
更新日期:2012-12-01 00:00:00
abstract::Birth defects (BDs) are functional and structural alterations in embryonic or fetal development. With an incidence of approximately 3-5%, BDs are a leading cause of infant mortality and lifelong disability. A population-based prospective case-control study was conducted for one year with 5204 infants, between March 1s...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0186
更新日期:2020-02-10 00:00:00
abstract::Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) m...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000047
更新日期:2009-07-01 00:00:00
abstract::Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1....
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000082
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abstract::Treatment of moderate and severe forms of osteogenesis imperfecta (OI) with cyclic pamidronate at the Reference Center for OI Treatment in Southern Brazil was studied. A retrospective cohort study was conducted from 2002 to 2012. Data were obtained during inpatient (drug infusion) and outpatient care. Clinical data, i...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0097
更新日期:2019-01-01 00:00:00
abstract::The order Anguilliformes comprises 15 families, 141 genera and 791 fish species. Eight families had at least one karyotyped species, with a prevalence of 2n = 38 chromosomes and high fundamental numbers (FN). The only exception to this pattern is the family Muraenidae, in which the eight species analyzed presented 2n ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000015
更新日期:2009-01-01 00:00:00
abstract::Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.6...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000036
更新日期:2009-04-01 00:00:00
abstract::The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A....
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0232
更新日期:2016-07-01 00:00:00
abstract::Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and D...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000043
更新日期:2012-07-01 00:00:00
abstract::The purpose of this study was to investigate whether treatment with electroacupuncture (EA) inhibited mitochondria-dependent apoptosis in annulus fibrosis (AF) cells in a rat model of cervical intervertebral disc degradation induced by unbalanced dynamic and static forces. Forty Sprague-Dawley rats were used in this s...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000046
更新日期:2012-07-01 00:00:00
abstract::We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000054
更新日期:2009-07-01 00:00:00
abstract::The web application D-Maps provides a user-friendly interface to researchers performing studies based on microarrays. The program was developed to manage and process one- or two-color microarray data obtained from several platforms (currently, GeneTAC, ScanArray, CodeLink, NimbleGen and Affymetrix). Despite the availa...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009000300030
更新日期:2009-07-01 00:00:00
abstract::During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013000200008
更新日期:2013-07-01 00:00:00