Abstract:
:In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce osteoblasts, chondrocytes and adipocytes. MSCs that differentiated into adipocytes were stimulated with and without bFGF. The OD/DNA (optical density/content of total DNA) and expression levels of the specific adipocyte genes PPARγ2 (peroxisome proliferator activated receptor γ2) and C/EBPs were higher in bFGF cells. Embryonic bodies had a higher adipocyte level compared with cells cultured in plates. These findings indicate that bFGF promotes adipocyte differentiation. MSCs may be useful cells for seeding in tissue engineering and have enormous therapeutic potential for adipose tissue engineering.
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
Song X,Li Y,Chen X,Yin G,Huang Q,Chen Y,Xu G,Wang Ldoi
10.1590/s1415-47572014000100019subject
Has Abstractpub_date
2014-03-01 00:00:00pages
127-34issue
1eissn
1415-4757issn
1678-4685journal_volume
37pub_type
杂志文章abstract::Now a days, an important and interesting alternative in the control of tick-infestation in cattle is to select resistant animals, and identify the respective quantitative trait loci (QTLs) and DNA markers, for posterior use in breeding programs. The number of ticks/animal is characterized as a discrete-counting trait,...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011005000049
更新日期:2011-10-01 00:00:00
abstract::Treatment of moderate and severe forms of osteogenesis imperfecta (OI) with cyclic pamidronate at the Reference Center for OI Treatment in Southern Brazil was studied. A retrospective cohort study was conducted from 2002 to 2012. Data were obtained during inpatient (drug infusion) and outpatient care. Clinical data, i...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0097
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abstract::The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A....
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0232
更新日期:2016-07-01 00:00:00
abstract::We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000054
更新日期:2009-07-01 00:00:00
abstract::Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A t...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000038
更新日期:2009-04-01 00:00:00
abstract::Xenobiotics can trigger degranulation of eosinophils and mast cells. In this process, the cells release several substances leading to bronchial hyperactivity, the main feature of atopic asthma (AA). GSTM1 and GSTT1 genes encode enzymes involved in the inactivation of these compounds. Both genes are polymorphic in huma...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010000300007
更新日期:2010-07-01 00:00:00
abstract::The order Anguilliformes comprises 15 families, 141 genera and 791 fish species. Eight families had at least one karyotyped species, with a prevalence of 2n = 38 chromosomes and high fundamental numbers (FN). The only exception to this pattern is the family Muraenidae, in which the eight species analyzed presented 2n ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000015
更新日期:2009-01-01 00:00:00
abstract::This study evaluated the diversity of cultivable plant growth-promoting (PGP) bacteria associated with apple trees cultivated under different crop management systems and their antagonistic ability against Colletotrichum gloeosporioides. Samples of roots and rhizospheric soil from apple trees cultivated in organic and ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000400013
更新日期:2014-09-01 00:00:00
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0186
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000096
更新日期:2010-01-01 00:00:00
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000028
更新日期:2013-09-01 00:00:00
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2017-0098
更新日期:2018-04-01 00:00:00
abstract::Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been karyotyped. Cytogenetic analyses performed on Genidens barbus and Sciades herzbergii revealed that both species have 2n ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000038
更新日期:2010-04-01 00:00:00
abstract::Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterize...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572014005000015
更新日期:2014-10-01 00:00:00
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0169
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011005000020
更新日期:2011-07-01 00:00:00
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0192
更新日期:2016-07-01 00:00:00
abstract::Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000040
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000100009
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abstract::Machado-Joseph disease (MJD) is an autosomal dominant, late-onset neurological disorder and the most common form of spinocerebellar ataxia (SCA) worldwide. Diagnostic genetic testing is available to detect the disease-causing mutation by direct sizing of the CAG repeat tract in the ataxin 3 gene. Presymptomatic testin...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000200012
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738320140376
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
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abstract::MicroRNAs are considered as potential biomarkers, agents, or therapeutic targets; few studies have addressed the expression of miRNAs in treatment-naïve patients infected with HIV-1. The aim of this study was to assess plasma relative circulating miRNA expression profiles in treatment-naïve Mexican patients with HIV/A...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
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更新日期:2020-06-22 00:00:00
abstract::The PPARγ nuclear receptor regulates the expression of genes involved in lipid and carbohydrate metabolism, and it has protective effects in some patients with type 2 diabetes. Nevertheless, the therapeutic value of the PPARγ nuclear receptor protein is limited due to the secondary effects of some PPARγ ligands. Becau...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000002
更新日期:2013-03-01 00:00:00
abstract::This review summarizes the chromosomal changes detected by molecular cytogenetic approaches in esophageal squamous cell carcinoma (ESCC), the ninth most common malignancy in the world. Whole genome analyses of ESCC cell lines and tumors indicated that the most frequent genomic gains occurred at 1, 2q, 3q, 5p, 6p, 7, 8...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000028
更新日期:2010-04-01 00:00:00
abstract::This study aimed to use gene chips to investigate differential gene expression profiles in the occurrence and development of acute myocardial infarction (AMI). The study included 12 AMI patients and 12 healthy individuals. Total mRNA of peripheral bloodwas extracted and reversed-transcribed to cDNA for microarray anal...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
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journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000082
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journal_title:Genetics and molecular biology
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更新日期:2011-01-01 00:00:00
abstract::The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011000400028
更新日期:2011-10-01 00:00:00
abstract::Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00