Abstract:
:The antitumorigenic potential of two palladium(II) complexes, [Pd(ca(2)-o-phen)Cl(2)] - C1 and [Pd(dmba)(dppp)Cl] - C2, was evaluated, using MDA-MB-435 cells, a human breast adenocarcinoma cell-line that does not express the estrogen receptor α (ER-). Growth inhibition and induced alterations in cell-morphology were analyzed. The sulforhodamine B test showed that, compared to control cells, both C1 and C2 significantly inhibited (p < 0.5) cell growth. The maximum effect with both was achieved with 1 μM complexes, after 24 h of treatment. No further cell-growth inhibition was achieved by increasing concentration or incubation time. Cell morphology was analyzed after staining with hematoxylin-eosin (HE). The morphological changes noted in the treated cells were cell rounding-up, shrinkage, nuclear condensation and reduction of cell length (p < 0.05), thereby indicating that both C1 and C2 are cytotoxic to breast adenocarcinoma cells. All together, there was every indication that, by decreasing cell growth and inducing morphological changes, the tested complexes are cytotoxic, hence their potentiality as promising candidates for antineoplastic drug development.
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
Campanella NC,da Silva Demartini M,Torres C,de Almeida ET,Gouvêa CMdoi
10.1590/s1415-47572012005000016subject
Has Abstractpub_date
2012-01-01 00:00:00pages
159-63issue
1eissn
1415-4757issn
1678-4685journal_volume
35pub_type
杂志文章abstract::Metachromatic leukodystrophy disorder (MLD) is an autosomal recessive and lysosomal storage disease. The disease is caused by the deficiency of the enzyme arylsulfatase A (ARSA) which is encoded by the ARSA gene. Different mutations have been reported in different populations. The present study was aimed to detect the...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0110
更新日期:2017-10-01 00:00:00
abstract::Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1....
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000082
更新日期:2009-10-01 00:00:00
abstract::The order Anguilliformes comprises 15 families, 141 genera and 791 fish species. Eight families had at least one karyotyped species, with a prevalence of 2n = 38 chromosomes and high fundamental numbers (FN). The only exception to this pattern is the family Muraenidae, in which the eight species analyzed presented 2n ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000015
更新日期:2009-01-01 00:00:00
abstract::This study presents evidence, using sequences of ribosomal 16S and COI mtDNA, for the presence of two mitochondrial genomes in Perumytilus purpuratus. This may be considered evidence of doubly uniparental mtDNA inheritance. The presence of the two types of mitochondrial genomes differentiates females from males. The F...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47573822201420140262
更新日期:2015-05-01 00:00:00
abstract::This study aimed to use gene chips to investigate differential gene expression profiles in the occurrence and development of acute myocardial infarction (AMI). The study included 12 AMI patients and 12 healthy individuals. Total mRNA of peripheral bloodwas extracted and reversed-transcribed to cDNA for microarray anal...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2017-0075
更新日期:2018-01-01 00:00:00
abstract::Alcohol dehydrogenases belong to the large superfamily of medium-chain dehydrogenases/reductases, which occur throughout the biological world and are involved with many important metabolic routes. We considered the phylogeny of 190 ADH sequences of animals, fungi, and plants. Non-class III Caenorhabditis elegans ADHs ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2017-0047
更新日期:2018-01-01 00:00:00
abstract::Jatropha gossypiifolia L. (Euphorbiaceae), popularly known as cotton-leaf physicnut, is a milky shrub notable for its medicinal properties. The present study aimed to evaluate the toxic, cytotoxic and genotoxic effects of the latex of J. gossypiifolia, using Allium cepa L. as test system. Seeds of A. cepa were exposed...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738120140162
更新日期:2015-03-01 00:00:00
abstract::Autochthonous pig breeds represent an important genetic reserve to be utilized mainly for the production of typical products. To explore its genetic variability, here we present for the first time whole genome sequencing data and SNPs discovered in a male domestic Nero Siciliano pig compared to the last pig reference ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0169
更新日期:2019-07-01 00:00:00
abstract::Rabies is a fatal zoonotic infection of the central nervous system of mammals and has been known to humans for millennia. The etiological agent, is a neurotropic RNA virus in the order Mononegavirales, family Rhabdoviridae, genus Lyssavirus. There are currently accepted to be two cycles for rabies transmission: the ur...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2019-0370
更新日期:2020-07-31 00:00:00
abstract::The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A....
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0232
更新日期:2016-07-01 00:00:00
abstract::Phylogenetic species of Paracoccidioides brasiliensis complex (S1a and S1b, PS2, PS3, and PS4) and Paracoccidioides lutzii are agents of paracoccidioidomycosis, an endemic fungal disease in Latin America. P. restrepiensis (PS3 genotype) was classified as monophyletic and geographically restricted to Colombia and neigh...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2019-0201
更新日期:2020-05-29 00:00:00
abstract::Birth defects (BDs) are functional and structural alterations in embryonic or fetal development. With an incidence of approximately 3-5%, BDs are a leading cause of infant mortality and lifelong disability. A population-based prospective case-control study was conducted for one year with 5204 infants, between March 1s...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0186
更新日期:2020-02-10 00:00:00
abstract::In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572012005000013
更新日期:2012-01-01 00:00:00
abstract::Oryza sativa, the common cultivated rice, is one of the most important crops for human consumption, but production is increasingly threatened by abiotic stresses. Although many efforts have resulted in breeding rice cultivars that are relatively tolerant to their local environments, climate changes and population incr...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0093
更新日期:2017-01-01 00:00:00
abstract::The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in Indi...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572011005000003
更新日期:2011-04-01 00:00:00
abstract::Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572013005000020
更新日期:2013-07-01 00:00:00
abstract::Exercise is a double-edged sword: when practiced in moderation, it increases the expression of antioxidant enzymes, but when practiced strenuously it causes oxidative stress and cell damage. In this context, polymorphisms in the interleukin (IL)-6 gene should be investigated better because they can influence performan...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0299
更新日期:2016-10-01 00:00:00
abstract::Lupinus albescens is a resistant cover plant that establishes symbiotic relationships with bacteria belonging to the Bradyrhizobium genus. This symbiosis helps the development of these plants in adverse environmental conditions, such as the ones found in arenized areas of Southern Brazil. This work studied three Brady...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2017-0098
更新日期:2018-04-01 00:00:00
abstract::Now a days, an important and interesting alternative in the control of tick-infestation in cattle is to select resistant animals, and identify the respective quantitative trait loci (QTLs) and DNA markers, for posterior use in breeding programs. The number of ticks/animal is characterized as a discrete-counting trait,...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011005000049
更新日期:2011-10-01 00:00:00
abstract::Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with l...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0312
更新日期:2017-07-01 00:00:00
abstract::The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade o...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000100009
更新日期:2014-03-01 00:00:00
abstract::Since the first diacylglycerol acyltransferase (DGAT) gene was characterized in plants, a number of studies have focused on understanding the role of DGAT activity in plant triacylglycerol (TAG) biosynthesis. DGAT enzyme is essential in controlling TAGs synthesis and is encoded by different genes. DGAT1 and DGAT2 are ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0024
更新日期:2016-10-01 00:00:00
abstract::Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterize...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572014005000015
更新日期:2014-10-01 00:00:00
abstract::Several phylogeographic studies of seabirds have documented low genetic diversity that has been attributed to bottleneck events or individual capacity for dispersal. Few studies have been done in seabirds on the Brazilian coast and all have shown low genetic differentiation on a wide geographic scale. The Kelp Gull is...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000065
更新日期:2012-12-01 00:00:00
abstract::Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs woul...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000091
更新日期:2009-10-01 00:00:00
abstract::Blood methylated cell-free DNA (cfDNA) as a minimally invasive cancer biomarker has great importance in cancer management. Guanylate binding protein 2 (GBP2) has been considered as a possible controlling factor in tumor development. GBP2 gene expression and its promoter methylation status in both plasma cfDNA and tumo...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2019-0230
更新日期:2020-11-17 00:00:00
abstract::The major histocompatibility complex (MHC) is one of the biological systems of major polymorphisms. The study of HLA class II variability has allowed the identification of several alleles that are characteristic to Amerindian populations, and it is an excellent tool to define the relations and biological affinities am...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009000200002
更新日期:2009-04-01 00:00:00
abstract::Rhagomys rufescens (Rodentia: Sigmodontinae) is an endemic species of the Atlantic forest from Southern and Southeastern Brazil. Some authors consider Rhagomys as part of the tribe Thomasomyini; but its phylogenetic relationships remain unclear. Chromosomal studies on eight specimens of Rhagomys rufescens revealed a d...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000071
更新日期:2010-07-01 00:00:00
abstract::The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorph...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000041
更新日期:2010-04-01 00:00:00
abstract::This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0297
更新日期:2017-10-01 00:00:00