Plasma GBP2 promoter methylation is associated with advanced stages in breast cancer.

Abstract:

:Blood methylated cell-free DNA (cfDNA) as a minimally invasive cancer biomarker has great importance in cancer management. Guanylate binding protein 2 (GBP2) has been considered as a possible controlling factor in tumor development. GBP2 gene expression and its promoter methylation status in both plasma cfDNA and tumor tissues of ductal carcinoma breast cancer patients were analyzed using SYBR green comparative Real-Time RT-PCR and, Methyl-specific PCR techniques, respectively in order to find a possible cancer-related marker. The results revealed that GBP2 gene expression and promoter methylation were inversely associated. GBP2 was down-regulated in tumors with emphasis on triple negative status, nodal involvement and higher cancer stages (p<0.0001). GBP2 promoter methylation on both cfDNA and tumor tissues were positively correlated and was detected in about 88% of breast cancer patients mostly in (Lymph node positive) LN+ and higher stages. Data provided shreds of evidence that GBP2 promoter methylation in circulating DNA may be considered as a possible effective non-invasive molecular marker in poor prognostic breast cancer patients with the evidence of its relation to disease stage and lymph node metastasis. However further studies need to evaluate the involvement of GBP2 promoter methylation in progression-free survival or overall survival of the patients.

journal_name

Genet Mol Biol

authors

Rahvar F,Salimi M,Mozdarani H

doi

10.1590/1678-4685-GMB-2019-0230

subject

Has Abstract

pub_date

2020-11-17 00:00:00

pages

e20190230

issue

4

eissn

1415-4757

issn

1678-4685

pii

S1415-47572020000600109

journal_volume

43

pub_type

杂志文章
  • Aromatase gene and its effects on growth, reproductive and maternal ability traits in a multibreed sheep population from Brazil.

    abstract::We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000054

    authors: Lôbo AM,Lôbo RN,Paiva SR

    更新日期:2009-07-01 00:00:00

  • ADP-ribosylation: from molecular mechanisms to human disease.

    abstract::Post-translational modification of proteins by ADP-ribosylation, catalysed by poly (ADP-ribose) polymerases (PARPs) using NAD+ as a substrate, plays central roles in DNA damage signalling and repair, modulates a range of cellular signalling cascades and initiates programmed cell death by parthanatos. Here, we present ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2019-0075

    authors: Hoch NC,Polo LM

    更新日期:2019-12-13 00:00:00

  • Diverse coactivator recruitment through differential PPARγ nuclear receptor agonism.

    abstract::The PPARγ nuclear receptor regulates the expression of genes involved in lipid and carbohydrate metabolism, and it has protective effects in some patients with type 2 diabetes. Nevertheless, the therapeutic value of the PPARγ nuclear receptor protein is limited due to the secondary effects of some PPARγ ligands. Becau...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572013005000002

    authors: Lizcano F,Vargas D

    更新日期:2013-03-01 00:00:00

  • Cytogenetical studies in five Atlantic Anguilliformes fishes.

    abstract::The order Anguilliformes comprises 15 families, 141 genera and 791 fish species. Eight families had at least one karyotyped species, with a prevalence of 2n = 38 chromosomes and high fundamental numbers (FN). The only exception to this pattern is the family Muraenidae, in which the eight species analyzed presented 2n ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000015

    authors: Vasconcelos AJ,Molina WF

    更新日期:2009-01-01 00:00:00

  • Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP.

    abstract::Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and D...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572012005000043

    authors: Raut AA,Kumar A,Kala SN,Chhokar V,Rana N,Beniwal V,Jaglan S,Samuchiwal SK,Singh JK,Mishra A

    更新日期:2012-07-01 00:00:00

  • Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology.

    abstract::The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/s1415-47572014005000010

    authors: Cassel AP,Barcellos RB,da Silva CM,de Matos Almeida SE,Rossetti ML

    更新日期:2014-06-01 00:00:00

  • Cyclic pamidronate treatment for osteogenesis imperfecta: Report from a Brazilian reference center.

    abstract::Treatment of moderate and severe forms of osteogenesis imperfecta (OI) with cyclic pamidronate at the Reference Center for OI Treatment in Southern Brazil was studied. A retrospective cohort study was conducted from 2002 to 2012. Data were obtained during inpatient (drug infusion) and outpatient care. Clinical data, i...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2018-0097

    authors: Pinheiro B,Zambrano MB,Vanz AP,Brizola E,Souza LT,Félix TM

    更新日期:2019-01-01 00:00:00

  • Frequency and origin of haplotypes associated with the beta-globin gene cluster in individuals with trait and sickle cell anemia in the Atlantic and Pacific coastal regions of Colombia.

    abstract::Sickle cell anemia is a genetic disease with high prevalence in people of African descent. There are five typical haplotypes associated with this disease and the haplotypes associated with the beta-globin gene cluster have been used to establish the origin of African-descendant people in America. In this work, we dete...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572013000400005

    authors: Fong C,Lizarralde-Iragorri MA,Rojas-Gallardo D,Barreto G

    更新日期:2013-12-01 00:00:00

  • Clinical evaluation of a substitute of HLA-B*58:01 in different Chinese ethnic groups.

    abstract::The goal of this research was to investigate the linkage disequilibrium between rs9263726 and HLA-B*58:01 in different Chinese ethnic groups (Han, Tibet, and Hui) and to study the feasibility of rs9263726 replacing HLA-B*58:01 as an efficient indicator of potential allopurinol hypersensitivity syndrome. In this study,...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2017-0258

    authors: Zhang X,Jin L,Wu Z,Ma W,Chen Y,Chen G,Wang L,Guan M

    更新日期:2018-07-01 00:00:00

  • Tools for Evolutionary and Genetic Analysis (TEGA): A new platform for the management of molecular and environmental data.

    abstract::Population genetics studies the distributions and changes in population allele frequencies in response to processes, such as mutation, natural selection, gene flow, and genetic drift. Researchers daily manage genetic, biological, and environmental data of the samples, storing them in text files or spreadsheets, which ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2018-0272

    authors: Elias DE,Rueda ECR

    更新日期:2020-05-29 00:00:00

  • An association study of FOXO3 variant and longevity.

    abstract::Human longevity is a polygenic and multifactorial trait. Pathways related to lifespan are complex and involve molecular, cellular, and environmental processes. In this analytical observational study, we evaluated the relationship between environment factors, oxidative stress status, DNA integrity level, and the associ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2017-0169

    authors: Silva-Sena GG,Camporez D,Santos LRD,Silva ASD,Sagrillo Pimassoni LH,Tieppo A,Pimentel Batitucci MDC,Morelato RL,Paula F

    更新日期:2018-04-01 00:00:00

  • Impact of co-blocking the costimulatory signals on immune-related genes after high-risk rabbit corneal allograft using 2nd-generation DNA sequencing technology.

    abstract::The aim of this study was to evaluate the impact and mechanism of co-blocking of costimulatory signals CD28-B7-CD40-CD40L during immune allograft rejection. Forty-eight recipient rabbits were prepared as a high-risk corneal allograft model. After surgery, the animals were randomly divided into: control group, MR1 grou...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2018-0150

    authors: Zhao HX,Li XY,Guan WY,Han XT

    更新日期:2019-04-01 00:00:00

  • Genotoxic effects of Tabebuia impetiginosa (Mart. Ex DC.) Standl. (Lamiales, Bignoniaceae) extract in Wistar rats.

    abstract::Tabebuia sp. is native to tropical rain forests throughout Central and South America. Although the biological and pharmacological effects of bark extracts have been intensely studied, little is known on the extract obtained from the flower. Herein, the genotoxic potential of a flower extract from T. impetiginosa ("ipê...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572012005000030

    authors: Lemos OA,Sanches JC,Silva IE,Silva ML,Vinhólis AH,Felix MA,Santos RA,Cecchi AO

    更新日期:2012-04-01 00:00:00

  • Bayesian inference in genetic parameter estimation of visual scores in Nellore beef-cattle.

    abstract::The aim of this study was to estimate the components of variance and genetic parameters for the visual scores which constitute the Morphological Evaluation System (MES), such as body structure (S), precocity (P) and musculature (M) in Nellore beef-cattle at the weaning and yearling stages, by using threshold Bayesian ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000066

    authors: de Faria CU,Koury W Jr,Magnabosco CU,de Albuquerque LG,Bezerra LA,Lôbo RB

    更新日期:2009-10-01 00:00:00

  • Introduction of the rd29A:AtDREB2A CA gene into soybean (Glycine max L. Merril) and its molecular characterization in leaves and roots during dehydration.

    abstract::The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drou...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572013000400015

    authors: Engels C,Fuganti-Pagliarini R,Marin SR,Marcelino-Guimarães FC,Oliveira MC,Kanamori N,Mizoi J,Nakashima K,Yamaguchi-Shinozaki K,Nepomuceno AL

    更新日期:2013-12-01 00:00:00

  • Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genetic polymorphisms and atopic asthma in children from Southeastern Brazil.

    abstract::Xenobiotics can trigger degranulation of eosinophils and mast cells. In this process, the cells release several substances leading to bronchial hyperactivity, the main feature of atopic asthma (AA). GSTM1 and GSTT1 genes encode enzymes involved in the inactivation of these compounds. Both genes are polymorphic in huma...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572010000300007

    authors: Lima CS,Néri IA,Lourenço GJ,Faria IC,Ribeiro JD,Bertuzzo CS

    更新日期:2010-07-01 00:00:00

  • bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells.

    abstract::In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce o...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/s1415-47572014000100019

    authors: Song X,Li Y,Chen X,Yin G,Huang Q,Chen Y,Xu G,Wang L

    更新日期:2014-03-01 00:00:00

  • Analysis of the CCR5 gene coding region diversity in five South American populations reveals two new non-synonymous alleles in Amerindians and high CCR5*D32 frequency in Euro-Brazilians.

    abstract::The CC chemokine receptor 5 (CCR5) molecule is an important co-receptor for HIV. The effect of the CCR5*D32 allele in susceptibility to HIV infection and AIDS disease is well known. Other alleles than CCR5*D32 have not been analysed before, neither in Amerindians nor in the majority of the populations all over the wor...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000011

    authors: Boldt AB,Culpi L,Tsuneto LT,Souza IR,Kun JF,Petzl-Erler ML

    更新日期:2009-01-01 00:00:00

  • Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences.

    abstract::Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.6...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000036

    authors: Lima RS Jr,Scarpassa VM

    更新日期:2009-04-01 00:00:00

  • Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae).

    abstract::Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000040

    authors: da Silva GM,Bione EG,Cabral-de-Mello DC,de Moura Rde C,Simões ZL,de Souza MJ

    更新日期:2009-04-01 00:00:00

  • Rabies virus diversification in aerial and terrestrial mammals.

    abstract::Rabies is a fatal zoonotic infection of the central nervous system of mammals and has been known to humans for millennia. The etiological agent, is a neurotropic RNA virus in the order Mononegavirales, family Rhabdoviridae, genus Lyssavirus. There are currently accepted to be two cycles for rabies transmission: the ur...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2019-0370

    authors: Oliveira RN,Freire CC,Iamarino A,Zanotto PM,Pessoa R,Sanabani SS,Souza SP,Castilho JG,Batista HBCR,Carnieli P Jr,Macedo CI,Watanabe JT,Brandão PE

    更新日期:2020-07-31 00:00:00

  • Overall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula.

    abstract::Heat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572012000200006

    authors: Soares-Cavalcanti NM,Belarmino LC,Kido EA,Pandolfi V,Marcelino-Guimarães FC,Rodrigues FA,Pereira GA,Benko-Iseppon AM

    更新日期:2012-06-01 00:00:00

  • Reciprocal regulation between alternative splicing and the DNA damage response.

    abstract::Splicing, the process that catalyzes intron removal and flanking exon ligation, can occur in different ways (alternative splicing) in immature RNAs transcribed from a single gene. In order to adapt to a particular context, cells modulate not only the quantity but also the quality (alternative isoforms) of their transc...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2019-0111

    authors: Botto AEC,Muñoz JC,Giono LE,Nieto-Moreno N,Cuenca C,Kornblihtt AR,Muñoz MJ

    更新日期:2020-03-27 00:00:00

  • The challenges of organizing an international course in Latin America.

    abstract::The Latin American School of Human and Medical Genetics (ELAG) is the main course of its kind in the genetics field in Latin America. Here we describe the main challenges regarding the organization of such event, including how we obtain funding and how we proceed with student selection. Thus, we aim to share our exper...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/s1415-47572014000200002

    authors: Vairo F,López ML,Cruz CU,Corrêa PG,Baldo G

    更新日期:2014-03-01 00:00:00

  • Tandem repeat distribution of gene transcripts in three plant families.

    abstract::Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs woul...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000091

    authors: da Maia LC,de Souza VQ,Kopp MM,de Carvalho FI,de Oliveira AC

    更新日期:2009-10-01 00:00:00

  • Successful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea.

    abstract::Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previous...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-475738320140376

    authors: Fávero AP,Dos Santos RF,Simpson CE,Valls JF,Vello NA

    更新日期:2015-07-01 00:00:00

  • Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models.

    abstract::The function of gene body DNA methylation in alternative splicing, and its relation to disease pathogenesis is not fully elucidated. The gene for familial Mediterranean fever (MEFV) encodes the pyrin protein and contains a 998 bp CpG island, covering the second exon, which is differentially methylated in FMF patients ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/1678-4685-GMB-2016-0234

    authors: Erdem GC,Erdemir S,Abaci I,Aydin AKK,Everest E,Turanli ET

    更新日期:2017-07-01 00:00:00

  • Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests.

    abstract::The common everyday use of medicinal plants is an ancient, and still very widespread practice, whereby the need for studies on their possible toxicity and mutagenic properties. The species Coccoloba mollis has been much used in phytotherapy, mainly in cases involving loss of memory and stress. In order to investigate ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572010005000062

    authors: Tsuboy MS,Marcarini JC,Ferreira DT,Ferraz ER,Chequer FM,de Oliveira DP,Ribeiro LR,Mantovani MS

    更新日期:2010-07-01 00:00:00

  • Conversion of barley SNPs into PCR-based markers using dCAPS method.

    abstract::Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) m...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572009005000047

    authors: Shahinnia F,Sayed-Tabatabaei BE

    更新日期:2009-07-01 00:00:00

  • Genetic evaluation of the mating system in the blue-and-yellow macaw (Ara ararauna, Aves, Psittacidae) by DNA fingerprinting.

    abstract::More than 90% of birds are socially monogamous, although genetic studies indicate that many are often not sexually monogamous. In the present study, DNA fingerprinting was used to estimate the genetic relationships between nestlings belonging to the same broods to evaluate the mating system in the socially monogamous ...

    journal_title:Genetics and molecular biology

    pub_type: 杂志文章

    doi:10.1590/S1415-47572010005000112

    authors: Caparroz R,Miyaki CY,Baker AJ

    更新日期:2011-01-01 00:00:00