DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser.

Abstract:

:This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a 'least concern' species. Genetic diversity did not differ significantly between noninvasive and invasive samples (Z(u) = 0.31, p = 0.37). Thus, noninvasive sampling, as demonstrated here with eggshells, provides an efficient means to assess genetic diversity in tropical birds without the need to capture and handle them.

journal_name

Genet Mol Biol

authors

Maia TA,Vilaça ST,Silva LRD,Santos FR,Dantas GPM

doi

10.1590/1678-4685-GMB-2016-0297

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

808-812

issue

4

eissn

1415-4757

issn

1678-4685

pii

S1415-47572017005025103

journal_volume

40

pub_type

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