Abstract：
:In an attempt to clarify the mechanism of sarcoplasmic reticulum (SR) dysfunction during the genesis of irreversible damage in the ischemic-reperfused myocardium, the changes in SR Ca2(+)-pumping ATPase (Ca2(+)-activated, Mg2(+)-dependent ATPase; Ca2(+)-ATPase) activity were studied during ischemia and subsequent reperfusion in the isolated perfused guinea pig heart preparation and correlated with the accumulation of calcium in the myocardium. Although the SR Ca2(+)-ATPase activity was not affected by ischemia of 40 min, reperfusion of the ischemic myocardium resulted in a definite time-dependent decrease in the enzyme activity. The reduction of SR Ca2(+)-ATPase activity was associated with a concomitant decrease in the enzyme concentration in the isolated SR and was in a good correlation with a substantial accumulation within the myocardium. As the results indicated the possibility that proteolytic degradation by a calcium-activated protease(s) was responsible for the reduction of enzyme activity, we examined for the possible involvement of calcium-activated neutral protease (CANP). However, SR Ca2(+)-ATPase obtained either from the normal hearts or from the hearts after 40-min ischemia was found to be quite resistant to proteolytic actions of the two forms of CANP, i.e., microCANP and mCANP partially purified from the guinea pig heart. These results suggest that a destructive process leading to the degradation of SR Ca2(+)-ATPase is activated by reperfusion, but not by ischemia per se, and that CANP is not implicated in the degradation of SR Ca2(+)-ATPase.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Yoshida Y,Shiga T,Imai Sdoi
10.1007/BF01931495subject
Has Abstractpub_date
1990-09-01 00:00:00pages
495-507issue
5eissn
0300-8428issn
1435-1803journal_volume
85pub_type
杂志文章abstract：:Implicit in attempts to characterize and purify biologically active factors is the premise that the bioassay system employed will show a progressive increase in the response as the concentration of the responsible factor increases. We employed the hamster cheek pouch to assay the neovascularization potential of growth...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907406
更新日期：1986-05-01 00:00:00
abstract：:The ability of the rhesus monkey to form coronary collaterals was tested in ten animals. Ameroid constrictors were implanted on the left circumflex coronary artery and allowed to remain for 12 weeks. One animal died of an acute myocardial infarction nine days after surgery; the remaining animals survived without clini...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906462
更新日期：1983-09-01 00:00:00
abstract：:In the chronically volume-overloaded canine heart due to AV-block evaluations of cardiac function were performed during the development of hypertrophy and at stable hypertrophy. In an early stage (1 and 2 weeks of AV-block) when no or only a slight increase of cardiac muscle occurred, contractility measured from dP/dt...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906357
更新日期：1977-03-01 00:00:00
abstract：:Mitochondrial dysfunction is an underlying cause of ischemia-reperfusion injury. In particular, ischemic injury induces dramatic increases in mitochondrial permeability, thereby instigating a chain of events that leads to both apoptotic and necrotic cardiomyocyte death. The mitochondrial permeability transition (MPT) ...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-009-0004-8
更新日期：2009-03-01 00:00:00
abstract：:Cardiac alpha- and beta-adrenoceptors and the positive inotropic effects of several adenylate cyclase dependent and independent agents have been measured in papillary muscle strips from patients without, as well as with moderate and severe heart failure. The number of beta-adrenoceptors was found to be decreased depen...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02650352
更新日期：1989-01-01 00:00:00
abstract：:Affinity labelling of the digitalis receptor has indicated that it is situated on the N-terminal part of the alpha-subunit of the (Na+,K+)ATPase. Biochemical and pharmacological properties of the (Na+,K+)ATPase studied on intact chick embryonic hearts and under heart cell culture conditions have indicated the existenc...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-642-72376-6_15
更新日期：1984-01-01 00:00:00
abstract：:During long-term treatment with amiodarone, slowing of conduction through the atrioventricular node, a prolongation of the QT-interval, and a prolongation of the atrial and ventricular myocardial refractoriness always developed. During short-term treatment, these effects were not found, except for depression of the AV...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02190546
更新日期：1991-03-01 00:00:00
abstract：:The assessment of endothelium-mediated modulation of coronary vasomotor tone in the intact human circulation under physiologic conditions requires very precise determination of both epicardial artery diameters, reflecting effects within the conduit vessels, as well as coronary blood flow, reflecting effects within the...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-72461-9_18
更新日期：1991-01-01 00:00:00
abstract：:The influence of perfusion pressure (5-8 kPa) on coronary flow and myocardial Ca2+-washout during Ca2+-free perfusion, and on the occurrence of calcium paradox after subsequent Ca2+-reperfusion, was studied in isolated, stimulated, rat heart ventricles. During the Ca2+-free perfusion period (3 min) the following chang...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907580
更新日期：1980-03-01 00:00:00
abstract：:Experiments were performed to determine the relative contributions of direct Ca(2+)-entry through the L-type Ca(2+)-current and of Ca(2+)-release from the sarcoplasmic reticulum (s.r.) to the intracellular [Ca2+]i-transient in isolated human atrial and ventricular myocytes from patients with severe heart failure and f...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00794074
更新日期：1997-01-01 00:00:00
abstract：:Myocardial ischemia-reperfusion (I/R) injury leads to intensive sympathetic nervous system (SNS) activation and inflammatory reactions. Whether renal sympathetic denervation (RDN) could be a new therapeutic strategy to modulate I/R inflammation and reduce infarct size after myocardial I/R injury needs to be explored. ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-020-0776-4
更新日期：2020-01-13 00:00:00
abstract：:The role of the cardiac current Ik1 in arrhythmogenesis remains highly controversal. To gain further insights into the mechanisms of IK1 involvement in cardiac excitability, we studied the susceptibility of transgenic mice with altered IK1 to arrhythmia during various pharmacological and physiological challenges. Arrh...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-007-0659-y
更新日期：2007-09-01 00:00:00
abstract：:To understand the effect of transcription regulation in modulating cardiac aging, we sought to study the role of serum response factor (SRF), a key transcription factor in the heart that is normally increased with senescence and also in congestive heart failure. A Tet-Off gene expression system was used for cardiac-sp...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-006-0634-z
更新日期：2007-05-01 00:00:00
abstract：:Isolated cells obtained from adult myocardium are proving invaluable for a wide range of studies concerned with the mechanisms underlying many important cardiological processes. Although care must always be taken to ensure that the single myocyte is an appropriate model for the particular question to be researched, it...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：
更新日期：1985-01-01 00:00:00
abstract：:Because of the close anatomical association between the ventricles, the volume of one ventricle can directly affect the volume and pressure within the other ventricle. To study the mechanical coupling between the ventricles, we modeled the right and left ventricles as a two-compartment model with right wall (Crw), sep...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907759
更新日期：1986-09-01 00:00:00
abstract：:Heart failure has been divided into several different forms depending on etiology, clinical course and pathophysiology of left ventricular (LV) dysfunction. Systolic and diastolic dysfunction are characterized by a reduced cardiac output with normal (= diastolic dysfunction) or depressed (= systolic dysfunction) LV pu...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/BF00795358
更新日期：1996-01-01 00:00:00
abstract：:Rat hearts were exposed and injured locally by direct cryoapplication. The hearts were examined 2, 4, 20 and 48 hours, 7 and 12 days later. In the first 48 hours mast cell and polymorphonuclear (mainly eosinophilic) granulocyte infiltration was followed by proliferation of mononuclear phagocytic and fibroblastic cells...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907742
更新日期：1979-05-01 00:00:00
abstract：:We have characterized the aerobic blood-perfused isolated heart model evaluating the hemodynamics and metabolism of both the blood donor animal and the isolated organ. Anaesthesia of the blood donor with sodium pentobarbital (30 mg/kg) increases arterial concentration of non esterified fatty acids (NEFA) from 80 +/- 6...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950050145
更新日期：1999-06-01 00:00:00
abstract：:The purpose of this study was to determine the effects of bradykinin (BK), substance P (SP) and histamine on plasma exudation in the skin of conscious dogs with and without pacing-induced heart failure. We also determined the role tissue angiotensin I-converting enzyme (ACE) and neutral endopeptidase (NEP) play in mod...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788284
更新日期：1994-09-01 00:00:00
abstract：:In the spontaneously hypertensive rat (SHR) with established hypertension left ventricular mass closely correlated with the proportion of ventricular myosin (VM)-3. A 60% VM-3 content was reached corresponding, under the present housing conditions, to an average age of 40 weeks without any significant change in total ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-11374-5_15
更新日期：1986-01-01 00:00:00
abstract：:The hypothesis was tested that vardenafil, a PDE5 inhibitor, specifically enhances coronary vasodilation during acidosis. In isolated constant pressure perfused guinea pig hearts, infusion of vardenafil ( journal_title：Basic research in cardiology pub_type： 杂志文章 doi：10.1007/s00395-006-0630-3 更新日期：2007-03-01 00:00:00
abstract：:The aim of this study was to investigate the mechanism of beta-adrenergic preconditioning (BPC). The roles of adenosine and its receptor subtypes, the generation of oxygen free radicals (ROS) and activation of the K(ATP) channels as well as the phosphoinositide-3-kinase (PI(3)K)/PKB/Akt and extracellular signal-regula...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0281-5
更新日期：2012-09-01 00:00:00
abstract：:The purpose of this study was to obtain more information about the role of the plasma membrane in cellular uptake of tracers used in myocardial scintigraphy (radioactive thallium chloride and palmitic acid). This knowledge is of importance for an adequate interpretation of the results obtained under clinical condition...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-11041-6_12
更新日期：1985-01-01 00:00:00
abstract：:It was previously reported that inhibition of carnitine synthesis by 3-(2,2,2-trimethyl-hydrazinium) propionate (MET-88) restores left ventricular (LV) systolic and diastolic function in rats with myocardial infarction (MI). Preservation of the calcium uptake function of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) is ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950070032
更新日期：2000-10-01 00:00:00
abstract：:MicroRNAs (miRNAs) are important regulators of adaptive and maladaptive responses in cardiovascular diseases and hence are considered to be potential therapeutical targets. However, their role as novel biomarkers for the diagnosis of cardiovascular diseases still needs to be systematically evaluated. We assessed here ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-010-0123-2
更新日期：2011-01-01 00:00:00
abstract：:Ca2+ overload followed by after-contractions was induced by perfusion of mammalian papillary muscles with O-K0+, high Ca2+ solution. The effect of lipotrophic agents (dexamethasone, indomethacin) or free radical scavengers (alpha-tocopherol, reduced glutathione, synthetic antioxidant) was tested at different stages of...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01908177
更新日期：1985-07-01 00:00:00
abstract：:Sympathetic neural remodeling, which involves the inflammatory response, plays an important role in ventricular arrhythmias (VAs) after myocardial infarction (MI). Adrenergic receptors on macrophages potentially modulate the inflammatory response. We hypothesized that the increased level of catecholamines activates ma...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-020-0813-3
更新日期：2020-08-07 00:00:00
abstract：:The concept of metabolic coronary flow control provides a rationale for the close relationship of coronary flow and myocardial metabolic rate of oxygen. The concept is based on the presence of an oxygen (metabolic) sensor coupled functionally to effector mechanisms, which control vascular tone. Four modes of metabolic...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-006-0621-4
更新日期：2006-11-01 00:00:00
abstract：:Short repeated cycles of peripheral ischemia/reperfusion (I/R) can protect distant organs from subsequent prolonged I/R injury; a phenomenon known as remote ischemic preconditioning (RIPC). A RIPC-mediated release of humoral factors might play a key role in this protection and vascular endothelial cells are potential ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-015-0474-9
更新日期：2015-03-01 00:00:00
abstract：:The article describes a method for monitoring the total energy output and oxygen uptake of isolated perfused rat hearts with working left ventricles. Twenty-two unpaced hearts (rates 4-4.5 bs-1) were separately investigated inside a flow micro-calorimeter (one minute for 90% thermal response) at 37 degrees C. They pum...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907920
更新日期：1985-09-01 00:00:00
