Abstract：
:We have characterized the aerobic blood-perfused isolated heart model evaluating the hemodynamics and metabolism of both the blood donor animal and the isolated organ. Anaesthesia of the blood donor with sodium pentobarbital (30 mg/kg) increases arterial concentration of non esterified fatty acids (NEFA) from 80 +/- 6 to 452 +/- 70 microM; p < 0.01. Injection of 1,000 U/kg heparin causes a second significant increase from 452 +/- 70 to 1012 +/- 104 microM; p < 0.01. Insertion of the perfusion circuit, without the isolated heart, causes a reduction in blood pressure of the blood donor and a significant increase in norepinephrine from 277 +/- 44 to 634 +/- 130 pg/ml; p < 0.05. Two hours of aerobic perfusion of the isolated heart inserted in the perfusion circuit, decreases arterial pressure of the blood donor with a concomitant increase of plasma norepinephrine from 475 +/- 150 to 841 +/- 159 pg/ml; p < 0.05. Developed pressure, oxygen consumption, glucose and NEFA uptake of the isolated heart remain constant during two hours of aerobic perfusion, NEFA being the preferred substrate. Tissue content of high energy phosphates at the end of the perfusion is high and similar to that observed "in vivo". Despite this, there is a release of lactate and CPK from the isolated heart. We conclude that: 1) the model allows accurate measurement of hemodynamics and metabolism of both the isolated heart and the blood donor animal; 2) the perfusion procedure modifies the substrates concentration of the blood donor animal which, in turn, results in the preferential NEFA utilization of the isolated heart. These changes do not affect the functional parameters of the perfused heart.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Pasini E,Solfrini R,Bachetti T,Marino M,Bernocchi P,Visioli F,Ferrari Rdoi
10.1007/s003950050145keywords:
subject
Has Abstractpub_date
1999-06-01 00:00:00pages
215-22issue
3eissn
0300-8428issn
1435-1803journal_volume
94pub_type
杂志文章abstract：:The stroke volume at rest of 81 test subjects with no evident cardiac or circulatory dysfunction was determined by impedance cardiography and the measurements were repeated after 11 weeks. In order to attain comparable and reproducible conditions, 44 test subjects were chosen who showed heart rates at rest not higher ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906800
更新日期：1978-11-01 00:00:00
abstract：:Ischaemic preconditioning is one of the most potent experimental modalities known to decrease infarct size after ischaemia and reperfusion. Much interest has been stimulated by the phenomenon of remote ischaemic conditioning (RIC), in which the preconditioning stimulus is applied to a limb remote from the heart to sti...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-013-0377-6
更新日期：2013-09-01 00:00:00
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journal_title：Basic research in cardiology
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doi：10.1007/s00395-006-0587-2
更新日期：2006-07-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Colloidal iron staining, calcium binding and enzyme activities were studied in the isolated rat heart sarcolemma. Colloidal iron staining of the sarcolemma revealed a high density of negatively charged sites associated with the cell surface. This membrane fraction was found to have calcium binding activity at both low...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01908160
更新日期：1981-01-01 00:00:00
abstract：:Since the discovery of a novel mechanism of cell death that differs from traditional necrosis, i.e., apoptosis, there have been numerous studies concluding that increased apoptosis augments myocardial infarction and heart failure and that limiting apoptosis protects the heart. Importantly, the vast majority of cells i...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-016-0549-2
更新日期：2016-05-01 00:00:00
abstract：:Alcoholic heart muscle disease is characterized by structural changes which include chamber dilation, ventricular hypertrophy, and myocyte damage. These effects often lead to contractile dysfunction and ultimately to heart failure if alcohol consumption is not terminated. In rat models for heart failure in which heart...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950050164
更新日期：1999-12-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0278-0
更新日期：2012-09-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-014-0459-0
更新日期：2015-03-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s003950050201
更新日期：1998-01-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01923191
更新日期：1983-01-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-014-0421-1
更新日期：2014-07-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-012-0296-y
更新日期：2012-09-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907406
更新日期：1986-05-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907051
更新日期：1987-01-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-015-0494-5
更新日期：2015-01-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-020-0797-z
更新日期：2020-05-22 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-008-0757-5
更新日期：2009-05-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-006-0634-z
更新日期：2007-05-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-017-0654-x
更新日期：2017-09-30 00:00:00
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journal_title：Basic research in cardiology
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journal_title：Basic research in cardiology
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doi：10.1007/s00395-009-0035-1
更新日期：2009-11-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788668
更新日期：1992-11-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/BF02070160
更新日期：1983-07-01 00:00:00
abstract：:Atrophy of the rat heart induced by hemodynamic unloading after heterotopic transplantation is associated with impaired relaxation while systolic function remains normal when compared to the heart of the recipient animal. To identify possible underlying mechanisms for the above, we studied some aspects of membrane cal...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788540
更新日期：1995-11-01 00:00:00
abstract：:A study of substrate selection in the isolated heart was made using 13C NMR isotopomer analysis, a method that unequivocally identifies relative substrate utilization. This technique has several advantages over conventional approaches used to study this problem. It detects the labeling of metabolic end-products presen...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00788500
更新日期：1995-09-01 00:00:00
abstract：:The hemodynamic and metabolic effects of acetate were studied in rats in vivo and in the isolated perfused heart. Hemodynamic parameters, myocardial phosphagens, inorganic phosphate, and adenosine were measured in vivo. Acetate uptake, coronary flow, O2 consumption, parameters of the cellular energy state, and hypoxan...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02005829
更新日期：1988-07-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s003950050207
更新日期：1998-01-01 00:00:00
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journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950200049
更新日期：2002-09-01 00:00:00
abstract：:Conflicting data on the inotropic effect of insulin are present in the literature suggesting a positive inotropic property or no inotropic effect or even a negative influence. To clarify the reason for these diverging findings, dose-response curves of insulin have been performed in isolated working rat and guinea pig ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-002-0350-2
更新日期：2002-07-01 00:00:00
