Abstract:
BACKGROUND:Antibodies to neuraminidase (NA) contribute to protection during influenza virus infection, but NA inhibition (NI) titers are not routinely analyzed in vaccine trials. One reason is the cumbersome nature of the conventional thiobarbituric acid (TBA) NI assay, which uses chemical methods to quantify free sialic acid following incubation of NA with substrate in the presence of serum. In addition, the assay is complicated by the need to use virus of a hemagglutinin (HA) subtype novel to the host to detect NA-specific antibodies only. OBJECTIVES:Our primary objectives were to miniaturize the colorimetric NI assay to a format suitable for quantitative analysis of large numbers of samples, and validate the specificity and sensitivity of the miniaturized format with ferret and human sera. An additional aim was to use reverse genetics to construct HA-mismatched viral reagents bearing NA of recent influenza A vaccine strains and H6 HA. RESULTS:Analysis of ferret antisera by the miniaturized assay demonstrated sensitivity and specificity comparable with the conventional assay. Similar increases in the NI titers in sera from vaccinated human volunteers were measured in miniaturized and conventional assays. Inactivated and live-attenuated vaccines increased NI titers against a given subtype at approximately the same rate. CONCLUSIONS:The reagents and miniaturized format of the TBA method described here provide a platform for practical serological monitoring of functional antibodies against NA.
journal_name
Influenza Other Respir Virusesjournal_title
Influenza and other respiratory virusesauthors
Sandbulte MR,Gao J,Straight TM,Eichelberger MCdoi
10.1111/j.1750-2659.2009.00094.xsubject
Has Abstractpub_date
2009-09-01 00:00:00pages
233-40issue
5eissn
1750-2640issn
1750-2659journal_volume
3pub_type
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/j.1750-2659.2008.00044.x
更新日期:2008-05-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,随机对照试验
doi:10.1111/irv.12204
更新日期:2014-01-01 00:00:00
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journal_title:Influenza and other respiratory viruses
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
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更新日期:2016-03-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/j.1750-2659.2010.00169.x
更新日期:2011-01-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,随机对照试验
doi:10.1111/irv.12608
更新日期:2019-01-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,meta分析
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更新日期:2020-07-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/irv.12754
更新日期:2020-09-01 00:00:00
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journal_title:Influenza and other respiratory viruses
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更新日期:2013-11-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,meta分析
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更新日期:2019-09-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/irv.12332
更新日期:2015-09-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,评审
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更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Highly pathogenic H5N1 influenza viruses reemerged in humans in 2003 and have caused fatal human infections in Asia and Africa as well as ongoing outbreaks in poultry. These viruses have evolved substantially and are now so antigenically varied that a single vaccine antigen may not protect against all circul...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/j.1750-2659.2012.00423.x
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Despite the use of vaccines, low-pathogenic (LP) H5N2 influenza viruses have continued to circulate and evolve in chickens in Mexico since 1993, giving rise to multiple genetic variants. Antigenic drift is partially responsible for the failure to control H5N2 influenza by vaccination; the contribution of mat...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/irv.12140
更新日期:2013-11-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,评审
doi:10.1111/irv.12406
更新日期:2016-11-01 00:00:00
abstract::Sinovac Biotech started to develop prototype pandemic influenza H5N1 vaccines in March 2004. On 2 April 2008, Sinovac's inactivated, aluminium-adjuvanted, whole-virion prototype pandemic influenza A (H5N1) vaccine (PanFlu) was granted production licensure by the China regulatory authority State Food and Drug Administr...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,随机对照试验,评审
doi:10.1111/j.1750-2659.2008.00067.x
更新日期:2008-11-01 00:00:00
abstract::Highly pathogenic avian influenza A H5N1 viruses remain a significant health threat to humans given the continued rare occurrence of human cases with a high case fatality rate. This brief literature review summarizes available evidence of risk factors for H5N1 infection in humans and updates a recent systematic review...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,评审
doi:10.1111/irv.12077
更新日期:2013-09-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/j.1750-2659.2009.00128.x
更新日期:2010-03-01 00:00:00
abstract:BACKGROUNDS:Influenza can spread rapidly in long-term care facilities (LTCFs), and residents are usually at higher risk for influenza infections. OBJECTIVE:Our study aimed to evaluate the effectiveness of antiviral interventions on outbreak control. METHODS:Taiwan Centers for Disease Control used a syndromic surveill...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/irv.12536
更新日期:2018-03-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/irv.12312
更新日期:2015-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/irv.12049
更新日期:2013-01-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/irv.12642
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Fever and respiratory symptoms are the major causes of hospitalisation in infants aged 90 days or less. Respiratory viruses (RVs) are detected by multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) in up to 70% of infants tested in this population. Aminotransferase elevation is not uncommon i...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/irv.12732
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:The new influenza virus A/H1N1 (2009), identified in mid-2009, rapidly spread over the world. Estimating the transmissibility of this new virus was a public health priority. METHODS:We reviewed all studies presenting estimates of the serial interval or generation time and the reproduction number of the A/H1...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,评审
doi:10.1111/j.1750-2659.2011.00234.x
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Modern molecular techniques reveal new information on the role of respiratory viruses in community-acquired pneumonia. In this study, we tried to determine the prevalence of respiratory viruses and bacteria in patients with community-acquired pneumonia who were admitted to the hospital. METHODS:Between Apri...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/j.1750-2659.2012.00425.x
更新日期:2013-07-01 00:00:00
abstract:OBJECTIVES:The goal of this double-blind, randomized, controlled clinical trial was to assess the safety and immunogenicity of two different doses of a monovalent split-virion 2009 pandemic influenza A/H1N1 vaccine without adjuvant in Chinese infants aged 6-35 months. DESIGN AND SETTING Subjects were randomly assigned...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章,随机对照试验
doi:10.1111/irv.12028
更新日期:2013-11-01 00:00:00
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journal_title:Influenza and other respiratory viruses
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/irv.12474
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:Recent outbreaks of highly pathogenic avian influenza and multiple occurrences of zoonotic infection and deaths in humans have sparked a dramatic increase in influenza research. In order to rapidly identify and help prevent future influenza outbreaks, numerous laboratories around the world are working to dev...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/j.1750-2659.2007.00031.x
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Research shows that unemployment reduces access to health care and vaccines and increases financial difficulty, family conflict, and other sources of stress that are known to suppress immune function. In addition, seasonal unemployment rates parallel seasonal influenza activity. Following a theory that argue...
journal_title:Influenza and other respiratory viruses
pub_type: 杂志文章
doi:10.1111/j.1750-2659.2011.00270.x
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:To estimate, using Bayesian evidence synthesis, the age-group-specific annual incidence of symptomatic infection with seasonal influenza in the Netherlands over the period 2005-2007. METHODS:The Netherlands population and age group distribution for 2006 defined the base population. The number of influenza-l...
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pub_type: 杂志文章
doi:10.1111/irv.12201
更新日期:2014-01-01 00:00:00