Abstract:
:AMPA receptors mediate fast excitatory neurotransmission by converting chemical signals into electrical signals, and thus it is important to understand the relationship between their chemical biology and their function. We used single-molecule fluorescence resonance energy transfer to examine the conformations explored by the agonist-binding domain of the AMPA receptor for wild-type and T686S mutant proteins. Each form of the agonist binding domain showed a dynamic, multistate sequential equilibrium, which could be identified only using wavelet shrinkage, a signal processing technique that removes experimental shot noise. These results illustrate that the extent of activation depends not on a rigid closed cleft but instead on the probability that a given subunit will occupy a closed-cleft conformation, which in turn is determined not only by the lowest energy state but also by the range of states that the protein explores.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Landes CF,Rambhadran A,Taylor JN,Salatan F,Jayaraman Vdoi
10.1038/nchembio.523subject
Has Abstractpub_date
2011-03-01 00:00:00pages
168-73issue
3eissn
1552-4450issn
1552-4469pii
nchembio.523journal_volume
7pub_type
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