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Metals induce transient folding and activation of the twister ribozyme.

Abstract：

:Twister is a small ribozyme present in almost all kingdoms of life that rapidly self-cleaves in variety of divalent metal ions. We used activity assays, bulk FRET and single-molecule FRET (smFRET) to understand how different metal ions promote folding and self-cleavage of the Oryza sativa twister ribozyme. Although most ribozymes require additional Mg2+ for catalysis, twister inverts this expectation, requiring 20-30 times less Mg2+ to self-cleave than to fold. Transition metals such as Co2+, Ni2+ and Zn2+ activate twister more efficiently than Mg2+ ions. Although twister is fully active in ≤ 0.5 mM MgCl2, smFRET experiments showed that the ribozyme visits the folded state infrequently under these conditions. Comparison of folding and self-cleavage rates indicates that most folding events lead to catalysis, which correlates with metal bond strength. Thus, the robust activity of twister reports on transient metal ion binding under physiological conditions.

journal_name

Nat Chem Biol

journal_title

Nature chemical biology

authors

Panja S,Hua B,Zegarra D,Ha T,Woodson SA

doi

10.1038/nchembio.2459

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

1109-1114

issue

10

eissn

1552-4450

issn

1552-4469

pii

nchembio.2459

journal_volume

13

pub_type

杂志文章

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