Abstract:
:The mechanism of DNA degradation and its clinical applications were examined. When purified lambda phage and extracted liver DNA were fixed in phosphate buffered formaldehyde, the DNA did not degrade, but there was incomplete digestion with endonuclease. Rat liver tissues were fixed under various conditions and DNA extracted. Immediate fixation with buffered formaldehyde at low temperature, or the addition of EDTA to buffered formaldehyde blocked the DNA degradation. Analysis of pulsed field gel electrophoresis also showed that DNA was degraded before extraction. These results suggest that tissue nuclease has an important role in DNA degradation in tissue. Furthermore, formaldehyde fixation at low temperature, which may take time and which decreases slightly the staining capacity, is useful for the extraction of intact DNA. For clinical application, the detection of provirus was examined. Genomic DNA was extracted from a necropsy sample of adult T cell leukaemia fixed in formaldehyde; human T cell leukaemia virus type-I (HTLV-I) provirus was successfully detected by Southern blotting.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Tokuda Y,Nakamura T,Satonaka K,Maeda S,Doi K,Baba S,Sugiyama Tdoi
10.1136/jcp.43.9.748subject
Has Abstractpub_date
1990-09-01 00:00:00pages
748-51issue
9eissn
0021-9746issn
1472-4146journal_volume
43pub_type
杂志文章abstract:AIMS:To investigate whether increasing the daily baseline of gut calcium can cause a gradual downregulation of the active intestinal transport of calcium via reduced parathyroid hormone (PTH) mediated activation of vitamin D, and to discuss why such a mechanism might prevent calcium oxalate rich stones. To demonstrate ...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/jcp.54.1.54
更新日期:2001-01-01 00:00:00
abstract::Operation specimens from 52 Hong Kong patients considered to have tuberculosis of the spine were sent at -78 degrees C to London where they were cultured on two Löwenstein-Jensen medium slopes, a slope of 7H11 medium made selective with antibiotics and in two bottles of selective liquid Kirchner medium. Cultures of M ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.6.662
更新日期:1983-06-01 00:00:00
abstract::The macrophage markers non-specific esterase, alpha 1-antitrypsin, alpha 1-antichymotrypsin, and lysozyme were compared with conventional microglial and macrophage stains in the human central nervous system. In a series of specimens from cases of head trauma, conventionally fixed and embedded, the modified Weil-Davenp...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.2.150
更新日期:1984-02-01 00:00:00
abstract:AIMS:The IHC4+C score combines assessment of oestrogen receptor (ER), progesterone receptor (PgR), HER2 and Ki67 with clinicopathological parameters to identify the risk of distant disease recurrence in patients with breast cancer, so, aiding treatment decision-making on adjuvant chemotherapy. Despite low cost and wide...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2015-203212
更新日期:2016-02-01 00:00:00
abstract::The coronavirus disease 2019 (COVID-19) is a zoonotic viral infection originating from Wuhan, China in December 2019. The World Health Organization has classified this pandemic as a global health emergency due to its virulent nature of transmission, which may lead to acute respiratory distress syndrome. Singapore's he...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206563
更新日期:2020-03-20 00:00:00
abstract::As patients with multiple myeloma (MM) have a variable clinical course, predictive markers would help determine the appropriate treatment strategy. Clinical staging is commonly used to predict outcome, but tumour marker expression and the underlying genetic changes are increasingly used to assess the biological aggres...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2007.049585
更新日期:2008-07-01 00:00:00
abstract::Several studies have recently documented the presence of persistently abnormal liver function tests in asymptomatic haemophiliacs. While the aetiology is unknown it is possible that repeated exposure to agents transmitted in blood products may be important. This study has attempted to determine the prevalence of viral...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.4.377
更新日期:1979-04-01 00:00:00
abstract:AIMS:To identify the amyloid protein in a patient with amyloidosis localised to the urinary bladder, and to see whether subtyping of the protein by sequence analysis increases the understanding of the selection of the urinary bladder as the site of amyloid deposition. METHODS:A patient with gross haematuria and a cong...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.54.12.920
更新日期:2001-12-01 00:00:00
abstract::The relation between the choline containing surfactant phospholipids lecithin and sphingomyelin in amniotic fluid and fetal lung maturity is well established. An enzymatic method that had been automated and optimised for use on a centrifugal analyser was used to measure the total choline containing phospholipids in am...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.11.1304
更新日期:1985-11-01 00:00:00
abstract::Two unusual ovarian tumours thought to be of Wolffian identity, one of them malignant, are described. They showed packed combinations of adenopapillary, tubular, trabecular and diffuse patterns, a sharp and generalised periodic acid-Schiff (PAS)-positive basement membrane and areas of elastic network. A review of publ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.5.526
更新日期:1982-05-01 00:00:00
abstract::The biochemical classification of the Klebsiella proposed by Cowan, Steel, Shaw, and Duguid (1960) has been applied to 50 strains isolated as the pure or predominant growth from sputum, and Klebsiella species have been correlated with the severity of the chest infection in the patient. A modification in nomenclature i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.15.5.478
更新日期:1962-09-01 00:00:00
abstract:AIMS:To determine whether the monoclonal antibody (MoAb) 83D4, previously shown to be highly specific for carcinoma cells, can be used as an immunocytological marker to discriminate between benign and malignant cells in serous effusions; and to test for a correlation between expression of the antigen reacting with MoAb...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.5.433
更新日期:1995-05-01 00:00:00
abstract::In an investigation of the in vitro phagocytic and microbicidal function of granulocytes collected by filtration leucapheresis (FL) from 18 donors and by discontinuous flow centrifugation leucapheresis (DFC) from six donors, comparison was made with the function of granulocytes obtained from the same donors by venepun...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.5.586
更新日期:1983-05-01 00:00:00
abstract:AIMS:To assess the occurrence of diagnostic delay in primary antibody deficiency in the period 1989-2002, since a similar study in 1989, and to assess the impact of UK national guidelines communicated in 1995. METHODS:A retrospective case note review was performed of 89 consecutive patients with antibody deficiency re...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.016204
更新日期:2005-05-01 00:00:00
abstract::The potencies of six commercially manufactured heparins have been measured by the British Pharmacopoeial (BP) assay and activated partial thromboplastin time (APTT), protamine sulphate, and anti-Xa assays. The APTT/BP potency ratios were found to vary with the preparation but this was not dependent on the tissue sourc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.6.562
更新日期:1980-06-01 00:00:00
abstract:INTRODUCTION:Large granular lymphocyte (LGL) leukaemia is considered a mature T-cell or natural killer (NK) cell neoplasm, characterised by a clonal proliferation of LGL. AIMS:To analyse the characteristics and to establish (if possible) the prognostic parameters of these patients diagnosed in a single centre: Univers...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-207191
更新日期:2021-01-21 00:00:00
abstract:AIMS:Primary cilia play an important role in the regulation of cell signalling pathways and are thought to have a role in cancer but have seldom been studied in human cancer samples. METHODS:Primary cilia were visualised by dual immunofluorescence for anti-CROCC (ciliary rootlet coiled-coil) and anti-tubulin in a rang...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-204103
更新日期:2017-07-01 00:00:00
abstract::An ultrastructural cytochemical study of lysosomal acid phosphatase was performed on leukemic cells in a case of chronic lymphocytic leukaemia of T cell origin (T-CLL). The cells showed inclusion bodies known as parallel tubular arrays, which often lay within acid phosphatase-positive, membrane-bound spaces. This sugg...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.2.167
更新日期:1982-02-01 00:00:00
abstract::The distribution and quantitative expression of HBcAg in relation to serum HBeAg and liver histology before and after a trial of interferon in 50 patients with chronic type B hepatitis were evaluated using polyclonal and monoclonal antibodies. In general, both antisera showed a similar pattern in terms of the distribu...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.44.1.21
更新日期:1991-01-01 00:00:00
abstract::A time and temperature relationship required to destroy certain pathogenic organisms in milk feeds has been ascertained. A method of achieving this destruction by using steam at atmospheric pressure is described, and a routine method for use in milk kitchens is suggested. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.4.321
更新日期:1966-07-01 00:00:00
abstract:AIM:Recent events surrounding postmortem practices have necessitated several changes in the way necropsies are conducted both within the medicolegal and hospital settings. These changes have resulted in a striking reduction in the frequency of brain retention for neuropathological examination. Aberdeen Royal Infirmary,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.028274
更新日期:2006-04-01 00:00:00
abstract::A system using totally disposable self-supporting bedpans requiring no carrier was examined in use in two hospitals. The bedpans and their contents were disposed of by destruction and flushing to waste carried out in a modified Haigh Sluicemaster disposal unit. This incorporates a positively closing and locking lid wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.12.925
更新日期:1973-12-01 00:00:00
abstract::Platelet transfusions have the highest incidence of post-transfusion sepsis compared with any other blood products. Recent reports suggest that platelet-related bacteraemia occurs at a frequency of approximately 50 times greater than that for red blood cells. The source is usually skin contaminants from the donor and ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.050492
更新日期:2010-03-01 00:00:00
abstract::A quantitative histological analysis of the major salivary glands was carried out at necropsy in 28 alcoholics and in a series of age and sex matched controls. The findings were related to the different types of histologically diagnosed liver disease present. Significant quantitative changes of salivary gland structur...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.8.837
更新日期:1988-08-01 00:00:00
abstract:AIMS:To use the polymerase chain reaction to detect monoclonality at the immunoglobulin heavy chain gene locus in cells derived from lymph node aspirates. METHODS:A nested two-stage polymerase chain reaction (PCR) for the VDJ region of the immunoglobulin heavy chain gene was used to detect monoclonality. The total num...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.5.420
更新日期:1992-05-01 00:00:00
abstract::A patient complained of severe diarrhoea for about four years. About 2.5 m of the jejunum was resected and duodenal polyps were also removed at laparotomy. Histologically, they were tubular or tubulovillus adenomas, which closely resembled colonic adenomas. No malignant change was detected in these adenomas. Severe in...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.9.981
更新日期:1986-09-01 00:00:00
abstract::The systematic screening of 253 children with transfusion-dependent homozygous beta-thalassaemia revealed a high incidence of hepatitis B virus markers. The highest frequencies of hepatitis B surface antigen (HBsAg) and antibody to hepatitis B core antigen (anti-HBc) were found in the group of patients with the smalle...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.10.949
更新日期:1980-10-01 00:00:00
abstract:BACKGROUND:For breast and prostate cancer, a gene expression signature of the tumour is associated with the development of distant metastases. Regarding head and neck squamous cell carcinoma (HNSCC), the only known risk factor is the presence of > or =3 tumour-positive lymph nodes. AIM:To evaluate whether a HNSCC gene...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.035451
更新日期:2006-12-01 00:00:00
abstract::A quantitative method of estimating heparin co-factor in terms of percentage activity is presented. The activity in sera from 75 normal subjects varied little from 100%. Low results were obtained in sera from 50 cases of varying types of malignancy. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.2.115
更新日期:1963-03-01 00:00:00
abstract::An elution technique is described which gives eluates at least as potent as previously described methods. Elution can be completed in only 15 minutes. The method, a modification of that of Vos and Kelsall, is simple to carry out and requires the minimum of equipment. Studies have shown that the coating antibody is alm...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.1.70
更新日期:1963-01-01 00:00:00