Abstract：
BACKGROUND:For breast and prostate cancer, a gene expression signature of the tumour is associated with the development of distant metastases. Regarding head and neck squamous cell carcinoma (HNSCC), the only known risk factor is the presence of > or =3 tumour-positive lymph nodes. AIM:To evaluate whether a HNSCC gene expression signature can discriminate between the patients with and without distant metastases. METHODS:Patients with HNSCC with and without distant metastases had >3 tumour-positive lymph nodes, and did not differ with respect to other risk factors. Statistical analysis was carried out using Student's t test, as well as statistical analysis of microarrays (SAM), to assess the false discovery rate for each gene. These analyses were supplemented with a newly developed method that computed deviations from gaussian-order statistics (DEGOS). To validate the platform, normal mucosa of the head and neck was included as control. RESULTS:2963 genes were differently expressed between HNSCC and normal mucosa (t test; p<0.01). More rigorous statistical analysis with SAM confirmed the differential expression of most genes. The comparison of genes in HNSCC with and without metastases showed 150 differently expressed genes (t test; p<0.01), none of which, however, could be confirmed using SAM or DEGOS. CONCLUSIONS:No evidence for a metastasis signature is found, and gene expression profiling of HNSCC has seemingly no value in determining the risk of developing distant metastases. The absence of such a signature can be understood when it is realised that, for HNSCC in contrast with breast cancer, the lymph nodes are a necessary in-between station for haematogenous spread.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Braakhuis BJ,Senft A,de Bree R,de Vries J,Ylstra B,Cloos J,Kuik DJ,Leemans CR,Brakenhoff RHdoi
10.1136/jcp.2005.035451subject
Has Abstractpub_date
2006-12-01 00:00:00pages
1254-60issue
12eissn
0021-9746issn
1472-4146pii
jcp.2005.035451journal_volume
59pub_type
杂志文章abstract：:The amyloid deposits in 21 renal biopsy specimens were subjected to a detailed immunohistochemical analysis using a panel of antibodies against recognised constituents of tissue amyloid. This was a retrospective study of material originally submitted during the investigation of various renal abnormalities and studied ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.44.3.200
更新日期：1991-03-01 00:00:00
abstract：:As part of a study to establish the prevalence of renal calculus disease, alkaline phosphatase activity was measured by the method recommended by the Scandinavian Society for Clinical Chemistry and Clinical Physiology in the plasma of 3823 adults from the Scottish new town of Cumbernauld. The enzyme activity differs i...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.31.12.1202
更新日期：1978-12-01 00:00:00
abstract：:The neutrophil shape change response to a chemotactic formylpeptide was assessed. Neutrophil bipolar shape formation (BSF) was also simultaneously assessed with a Boyden chamber-based neutrophil migration assay. Both assays were precise and relatively reproducible; the average coefficient of variation for the BSF assa...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.43.4.342
更新日期：1990-04-01 00:00:00
abstract：:Mast cells were identified by electronmicroscopy in the alveolar wall of the lung in 20 subjects (10 normal, 10 abnormal). A quantitative and qualitative study was made of the mast cells. In the normal lung there was an average concentration of 350 mast cells/mm2 of alveolar wall and in the abnormal 523/mm2. Mast cell...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.34.12.1333
更新日期：1981-12-01 00:00:00
abstract：:The use of the dye calcon (1-(2 hydroxy-1-naphthylazo)-2-naphthol-4 sulphonic acid) for the estimation of calcium using E.D.T.A. and a commercial photoelectric titrimeter is described. The interfering effects of magnesium and phosphate have been overcome. The method has been extended to estimations on biological mater...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.16.1.18
更新日期：1963-01-01 00:00:00
abstract：AIMS/BACKGROUND:A major medical role for postmortem examinations is the detection of clinically unexpected disease processes contributing to death. The aim of the present study was to determine whether simple clinical parameters can predict the presence of important unanticipated findings at necropsy. METHODS:Prospect...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.49.11.909
更新日期：1996-11-01 00:00:00
abstract：:The sellar region is the site of a large number of pathological entities arising from the pituitary and adjacent anatomical structures, including brain, blood vessels, nerves and meninges. The surgical pathology of this area requires the accurate identification of neoplastic lesions, including pituitary adenoma and ca...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.2005.031187
更新日期：2006-12-01 00:00:00
abstract：:A histochemical method is described of differentiating between normal and neoplastic cells in plasma using the activity of various enzymes characteristic of plasma cells. It was found that in both types of cell increased or diminished activity of cytoenzymes is a useful means of distinguishing between normal and abnor...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.18.3.345
更新日期：1965-05-01 00:00:00
abstract：AIMS:The development of a technique for simultaneous in situ hybridisation for native mRNA and conventional immunofluorescence for cytoplasmic antigens in routine pathology specimens. METHODS:Cocktails of synthetic deoxyoligonucleotides coding for immunoglobulin J chain and kappa light chain were 3' end labelled enzym...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.45.2.114
更新日期：1992-02-01 00:00:00
abstract：:Sixty-nine specimens of liver tissue from 53 infants with neonatal hepatitis or its sequelae were examined without knowledge of the alpha1-antitrypsin phenotype. Distinctive, diastase-resistant, PAS-positive, pure magenta-coloured, sharply defined globules, 2-20 microns in diameter were found in periportal and parasep...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.28.7.559
更新日期：1975-07-01 00:00:00
abstract：:A case is described in which marked hepatic fibrosis probably resulted from the prolonged treatment of acute leukaemia with methotrexate. ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.19.1.81
更新日期：1966-01-01 00:00:00
abstract：:The arrangement of the T cell receptor and immunoglobulin genes was analysed in lymphoid tissue biopsy specimens from 25 cases of angioimmunoblastic lymphadenopathy. Nineteen cases showed a rearrangement of the gene coding for the beta chain of the T cell receptor, and in one case a clonal rearrangement of immunoglobu...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.39.11.1229
更新日期：1986-11-01 00:00:00
abstract：:A case of bilateral gonadoblastoma/dysgerminoma in a 46 XY phenotypical female is presented. Increased circulating beta human chorionic gonadotropin (beta-HCG) and testosterone together with a decreased concentration of follicle stimulating hormone (FSH) reflected the activities of these tumours. The patient is alive ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.35.4.420
更新日期：1982-04-01 00:00:00
abstract：:The results of the measurement of vitamin B(12) absorption by counting the radioactivity of 5 ml. serum obtained eight to 10 hours after the ingestion of an oral dose of 0.5 mug. vitamin B(12) labelled with 0.5 muc. (57)Co are compared with those obtained with the urinary excretion (Schilling) test. Inadequate urine c...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.19.6.606
更新日期：1966-11-01 00:00:00
abstract：:The cytological features of testicular germ cell tumours were established in smears from 15 freshly resected tumours. These features were applied to the fine needle aspiration cytology diagnosis of metastases in 27 patients referred for chemotherapy. There were 16 positive reports in 32 aspirates of which 13 were take...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.40.11.1324
更新日期：1987-11-01 00:00:00
abstract：:The histological appearances of the breast in a patient with mucoviscidosis are those of complete lobular agenesis. Ducts and ductules are well developed, and there is abundant fibrous tissue giving the breast a normal contour. The appearances are contrasted with those of gynaecomastia, and the aetiology is discussed....
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.25.2.119
更新日期：1972-02-01 00:00:00
abstract：:A Cyto-Tek staining machine designed for Papanicolau staining of cervical smears has been adapted for auramine staining of smears of sputum and other clinical specimens for acid-fast bacilli. The results compared favourably with those obtained by a manual method of staining. Contamination of negative smears by acid-fa...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.31.2.185
更新日期：1978-02-01 00:00:00
abstract：:A 45-year-old female patient underwent right hemihepatectomy for metastatic rectal adenocarcinoma. Preoperative imaging demonstrated an area of focal nodular hyperplasia (FNH) in segment VIII and metastatic carcinoma in segment VI of the liver. Gross and microscopic examination of the former lesion showed features typ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.55.12.967
更新日期：2002-12-01 00:00:00
abstract：:The ability of erythrocytes to synthesize porphyrins from delta-aminolaevulic acid was compared in healthy people, patients with true iron-deficiency anaemia, and anaemic patients with rheumatoid arthritis. Porphyrin synthesis was found to be impaired in true iron deficiency but not in rheumatoid arthritis cases showi...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.24.4.336
更新日期：1971-05-01 00:00:00
abstract：:The Human Leukocyte Antigen (HLA) system plays a critical role in regulating the immune response. As a consequence of its role in immune regulation and exquisite polymorphism, the HLA system also constitutes an immunological barrier which must be avoided or otherwise overcome in clinical transplantation. This introduc...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.2009.072371
更新日期：2010-05-01 00:00:00
abstract：:Sperm-agglutinating antibodies were present in three (2-6%) of 117 fertile men and in 19 (33-9%) of 56 men who had been vasectomized. Twenty-four of the 56 vasectomized men had been studied before vasectomy; sperm-agglutinating antibodies were present in one (4-3%) compared with eight (33-3%) after vasectomy. No sperm...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.29.12.1127
更新日期：1976-12-01 00:00:00
abstract：AIMS:To investigate the role of needle core biopsy (NCB) in the preoperative assessment of impalpable breast lesions, mainly derived from the NHS Breast Screening Programme (NHSBSP) and to assess our own modifications to a suggested system for the classification of breast NCBs. METHODS:The NCB, fine needle aspiration ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.54.2.121
更新日期：2001-02-01 00:00:00
abstract：:Pathological findings are described in four cases of a new aminoaciduria in which homocystine is excreted in the urine. All the patients were mentally retarded children. Three of them presented diagnostic features of Marfan's syndrome. Necropsy on one case and biopsy findings in the others are described. Fatty change ...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.17.4.427
更新日期：1964-07-01 00:00:00
abstract：:In studies using indirect immunofluorescence IgG antibodies to Clostridium perfringens were found in sera from healthy adults. Sera from 236 healthy children were examined. The normally occurring IgG antibodies to C perfringens were found to have an age related variation. Preliminary data suggest that they are not cor...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.40.3.282
更新日期：1987-03-01 00:00:00
abstract：AIM:Thalassaemia is a good candidate disease for control by preventive genetic programmes in developing countries. Accurate population frequency data are needed for planning the control of thalassaemia in the high risk Guangdong Province of southern China. METHODS:In total, 13397 consecutive samples from five geograph...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2003.014456
更新日期：2004-05-01 00:00:00
abstract：:A questionnaire was circulated to clinical biochemistry laboratories in the North West Thames region of the United Kingdom requesting information on extralaboratory equipment. Data on the types and numbers of instruments in use, their relationship with the laboratory, and quality assurance procedures were obtained. La...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.41.9.925
更新日期：1988-09-01 00:00:00
abstract：AIMS:The chemokine receptor CXCR7 is found on glioma cells and glioma-associated vessels and dependent upon its localisation on tumour or endothelial cells the CXCR7 receptor can mediate glioma cell invasion and tumour angiogenesis. Its expression predicts survival in several types of cancers. METHODS:We immunohistoch...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jclinpath-2015-202886
更新日期：2015-10-01 00:00:00
abstract：:Increasingly in the field of medicine, new therapeutic modalities, both surgical and non-surgical, are being introduced. Some of these may significantly alter the pathological appearance of normal and neoplastic tissue and result in problems for the pathologist. In this review, iatrogenic pathology within the female g...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.2008.061424
更新日期：2009-02-01 00:00:00
abstract：:Serious infection caused by Lancefield group C streptococci is unusual in man. Two unrelated deaths associated with these organisms in a 55 year old woman who died after three days of diarrhoea and vomiting, and in a 65 old man who died after a week of non-specific symptoms, are presented. Post mortem examination show...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.46.10.965
更新日期：1993-10-01 00:00:00
abstract：AIM:To evaluate the sources of fetal haemoglobin (HbF) as an indicator in cancer. An immunohistochemical study was carried out on some of the most common kinds of cancer. All of these cancers had serologically high levels of HbF as evaluated previously. METHODS:Immunoaffinity-purified anti-HbF was immunohistochemicall...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.2005.029934
更新日期：2006-06-01 00:00:00
