Abstract:
AIMS:Primary cilia play an important role in the regulation of cell signalling pathways and are thought to have a role in cancer but have seldom been studied in human cancer samples. METHODS:Primary cilia were visualised by dual immunofluorescence for anti-CROCC (ciliary rootlet coiled-coil) and anti-tubulin in a range of human cancers (including carcinomas of stomach, pancreas, prostate, lung and colon, lobular and ductal breast cancers and follicular lymphoma) and in matched normal tissue (stomach, pancreas, lung, large and small intestines, breast and reactive lymph nodes) samples using a tissue microarray; their frequency, association with proliferation, was measured by Ki-67 staining and their structure was analysed. RESULTS:Compared with normal tissues, primary cilia frequency was significantly elevated in adenocarcinoma of the lung (2.75% vs 1.85%, p=0.016), adenocarcinoma of the colon (3.80% vs 2.43%, respectively, p=0.017), follicular lymphoma (1.18% vs 0.83%, p=0.003) and pancreatic adenocarcinoma (7.00% vs 5.26%, p=0.002); there was no statistically significant difference compared with normal control tissue for gastric and prostatic adenocarcinomas or for lobular and ductal breast cancers. Additionally, structural abnormalities of primary cilia were identified in cancer tissues, including elongation of the axoneme, multiple basal bodies and branching of the axoneme. Ki-67 scores ranged from 0.7% to 78.4% and showed no statistically significant correlation with primary cilia frequency across all tissues (p=0.1501). CONCLUSIONS:The results show upregulation of primary cilia and the presence of structural defects in a wide range of human cancer tissue samples demonstrating association of dysregulation of primary cilia with human cancer.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Yasar B,Linton K,Slater C,Byers Rdoi
10.1136/jclinpath-2016-204103subject
Has Abstractpub_date
2017-07-01 00:00:00pages
571-574issue
7eissn
0021-9746issn
1472-4146pii
jclinpath-2016-204103journal_volume
70pub_type
杂志文章abstract::When the posture was changed from horizontal to vertical, or the reverse, the alteration in plasma volume and in the levels of haematocrit, haemoglobin, and plasma protein was much greater in patients with oedema or low plasma protein or albumin concentrations ("the pathological group") than in patients without these ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.13.4.304
更新日期:1960-07-01 00:00:00
abstract::Five cases of malignant histiocytosis of the intestine and one case of true histiocytic lymphoma were studied using immunohistological techniques. In paraffin sections tumour cells in all cases were shown to contain alpha-1-antitrypsin and to express the leucocyte common antigen. Four of the five cases of malignant hi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.1.8
更新日期:1986-01-01 00:00:00
abstract::In a post-mortem analysis of 1,500 patients, aged 70 years or more, heart disease and malignancy were the two major causes of death. Coronary disease was exceedingly prevalent, almost one in five being subject to this form of heart disease, and 13% dying from its effects. The levelling of the sex ratio in coronary dis...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.6.532
更新日期:1963-11-01 00:00:00
abstract::The sensitivity of an indirect immunofluorescence antibody test (IFAT) and a rapid microagglutination test (RMAT) for the diagnosis of Legionnaires' disease caused by Legionella pneumophila serogroup 1 was evaluated using serum specimens from 119 patients with bacteriologically confirmed infections. The sensitivity of...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.1.77
更新日期:1987-01-01 00:00:00
abstract::Two examples of intraplacental choriocarcinoma are described. Both were small and had arisen in otherwise normal third trimester placentas. The covering mantle of many of the villi adjacent to the choriocarcinomas was formed, either focally or wholly, of neoplastic trophoblastic tissue: it is only at this stage of the...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.10.1085
更新日期:1988-10-01 00:00:00
abstract:AIMS:To test the hypothesis that a correct qualitative assessment of axillary nodal status can be established by examining only a limited number of lymph nodes. METHODS:Slides from 499 pN1 or pN0 axillary dissection specimens relating to symptomatic breast cancer cases operated on at our institution between 1991 and 1...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.9.681
更新日期:1999-09-01 00:00:00
abstract::A commercial latex slide test (Oxoid DR 620) for the identification of Escherichia coli O157 was compared with a standard tube agglutination method using E coli O157 antiserum (Difco). Thirty isolates previously confirmed as E coli O157 were positive by both methods: 30 isolates of E coli of serogroups other than O157...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.10.1109
更新日期:1989-10-01 00:00:00
abstract::A new sensitive nitrite test, the SRN test, was evaluated for its suitability as a reliable screening technique for the detection of bacteriuria. The SRN test was compared to a currently available nitrite test, the Microstix nitrite test, and the results obtained with both nitrite tests were assessed in comparison wit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.7.723
更新日期:1981-07-01 00:00:00
abstract::Components of the blood fibrinolytic system were measured in 43 patients with leukaemia. Increased fibrinolytic activity, indicated by a reduced plasma plasminogen level, was found in 14 patients. The combination of severe thrombocytopenia and increased fibrinolysis appeared to be a determinant in the production of a ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.2.136
更新日期:1968-03-01 00:00:00
abstract::A patient complained of severe diarrhoea for about four years. About 2.5 m of the jejunum was resected and duodenal polyps were also removed at laparotomy. Histologically, they were tubular or tubulovillus adenomas, which closely resembled colonic adenomas. No malignant change was detected in these adenomas. Severe in...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.9.981
更新日期:1986-09-01 00:00:00
abstract::A biochemical typing method is described for Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes. The technique depends on differences in metabolism of five carbon substrates--glycerol, inositol, lactose, glucose, and xylose--at two concentrations. Reproducibility is satisfactory and is monitored by the inco...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.9.935
更新日期:1979-09-01 00:00:00
abstract::There is growing evidence of histopathological changes in autopsied individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, data on histopathological changes in autopsied patients with eradicated COVID-19 are limited. We performed an autopsy on a Caucasian female in her 80s, wh...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206879
更新日期:2020-07-28 00:00:00
abstract::Three hundred specimens of oesophagus obtained at necropsy from infants and children aged from 0 to 14 years, dying from a variety of causes, were examined prospectively for evidence of gastric heterotopia. Gastric heterotopia was observed in 63 (21%) of the whole series, representing a much higher incidence than repo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.7.742
更新日期:1988-07-01 00:00:00
abstract:AIMS:To institute recommendations from a laboratory turnround time study; to evaluate audit methods; and to quantify improvements achieved. METHODS:Changes to result report distribution and specimen delivery were affected by posting results directly from the laboratory followed by the introduction of a twice daily cou...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.4.372
更新日期:1995-04-01 00:00:00
abstract::In vitro morphological and functional studies were carried out on platelets which had been cryopreserved in the presence of 5% dimethyl sulphoxide (DMSO). Overall loss of platelets was around 50%. Those which survived freezing and reconstitution showed marked morphological deterioration, increase of procoagulant activ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.8.870
更新日期:1982-08-01 00:00:00
abstract::Human epidermal growth factor receptor 2 (HER2) testing is required for newly diagnosed breast cancer and advised for recurrent and metastatic breast cancer, to determine treatment planning using HER2-directed therapy in the neoadjuvant, adjuvant and advanced disease settings. Wide variation, nationally, in the turnar...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201819
更新日期:2014-02-01 00:00:00
abstract::The cytological features of testicular germ cell tumours were established in smears from 15 freshly resected tumours. These features were applied to the fine needle aspiration cytology diagnosis of metastases in 27 patients referred for chemotherapy. There were 16 positive reports in 32 aspirates of which 13 were take...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.11.1324
更新日期:1987-11-01 00:00:00
abstract:AIMS:To determine the clinical performance of three cerebrospinal fluid (CSF) IgG synthesis formulae using data obtained from two quantitation methods. METHODS:Receiver operator characteristic (ROC) analysis and decision index plots were used to compare a rate nephelometric (RN) and a rocket immunoelectrophoretic (RIE...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.1.24
更新日期:1996-01-01 00:00:00
abstract::A clinical study of 401 cases of tumour of salivary tissue has been made and the results reported, together with a review of the literature. The histological variations of such tumours are classified and discussed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.14.3.232
更新日期:1961-05-01 00:00:00
abstract:AIM:Heart failure (HF) affects millions of older individuals in both developed and low/middle-income countries. Serum galectin-3 levels have been shown to have prognostic value. However, its use as a diagnostic biomarker has not been explored. The aim was to establish a saliva galectin-3 reference range and to demonstr...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203631
更新日期:2016-12-01 00:00:00
abstract::The discovery of ETS gene fusions as common events in prostate cancer represents a paradigmatic shift in the significance attributed to chromosomal translocations as a key mechanistic player in carcinogenesis. However, these chromosomal fusion events are poorly understood, as their functional significance and therapeu...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2008.056341
更新日期:2008-08-01 00:00:00
abstract::Pseudomonas cepacia (Ps. multivorans) can become adapted to a 1 in 30 dilution of Savlon hospital concentrate in distilled water, and can multiply in it. The organism so adapted did not survive in Savlon of the same strength made with hard tap water (pH 7.2). This effect was due to the difference of pH between the two...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.8.708
更新日期:1971-11-01 00:00:00
abstract::The air of three hospital wards was examined frequently for staphylococci for a period of nearly four years using slit samplers. ;Broadcasts' of staphylococci into the air were observed with the air count rising well above the mean of 0.2 staphylococcal particle per cubic foot and these broadcasts often appeared to be...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.15.6.552
更新日期:1962-11-01 00:00:00
abstract:AIMS:To estimate the expression of substance P in the haematopoietic cells of hypoplastic bone marrow and define its relationship with the course of bone marrow hypoplasia. METHODS:Bone marrow specimens were obtained from 42 children with bone marrow hypoplasia who were hospitalised in the Department of Pediatric Onco...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.034629
更新日期:2006-09-01 00:00:00
abstract::A case of florid rheumatoid heart disease with complete heart block is described, and the histopathology of the atrioventricular conducting tissue is described in detail. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.7.623
更新日期:1970-10-01 00:00:00
abstract::The histological appearances of the breast in a patient with mucoviscidosis are those of complete lobular agenesis. Ducts and ductules are well developed, and there is abundant fibrous tissue giving the breast a normal contour. The appearances are contrasted with those of gynaecomastia, and the aetiology is discussed....
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.2.119
更新日期:1972-02-01 00:00:00
abstract::Two unusual ovarian tumours thought to be of Wolffian identity, one of them malignant, are described. They showed packed combinations of adenopapillary, tubular, trabecular and diffuse patterns, a sharp and generalised periodic acid-Schiff (PAS)-positive basement membrane and areas of elastic network. A review of publ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.5.526
更新日期:1982-05-01 00:00:00
abstract:AIMS:Sinusoidal dilatation and congestion (SDC) in liver biopsy may be obstructive (due to venous outflow impairment) or non-obstructive in nature. The significance of this finding in the post-liver transplant setting remains unexplored. METHODS:We herein retrospectively analysed all post-transplant liver biopsies sho...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206870
更新日期:2020-08-24 00:00:00
abstract::Platelet concentrates were prepared for in vitro storage in either Fenwal PL-732 or Cutter CLX platelet packs. The units were stored at 22 degrees C for seven days with either horizontal or tumbler agitation. Measurement of pH, hypotonic shock response and serotonin uptake indicated in vitro viability was well maintai...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.12.1382
更新日期:1983-12-01 00:00:00
abstract::Of the first 250 heart and 35 heart and lung transplant recipients at Papworth Hospital, Cambridge, who survived for more than one month after transplantation, 217 heart and 33 heart and lung patients were investigated serologically for evidence of Toxoplasma gondii infection. Six patients acquired primary T gondii in...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.2.194
更新日期:1989-02-01 00:00:00