An evaluation of the British Pharmacopoeial assay of heparin: a comparison with other methods.

Abstract:

:The potencies of six commercially manufactured heparins have been measured by the British Pharmacopoeial (BP) assay and activated partial thromboplastin time (APTT), protamine sulphate, and anti-Xa assays. The APTT/BP potency ratios were found to vary with the preparation but this was not dependent on the tissue source of heparin. For mucosal heparins, the anti-Xa/BP potency ratios were close to unity, but for heparin of lung origin the anti-Xa potency was approximately one-quarter of the BP potency. Four heparin fractions prepared by column gel chromatography of a commercial heparin were similarly examined by all four assays, and there was a wide divergence between the BP potency estimates and those obtained with the other methods. The degree of divergence was found to depend on the molecular size of the fraction.

journal_name

J Clin Pathol

authors

Shah GA,Dhall TZ,Ferguson I,Davis MR,Graham DT,Pomeroy AR,Dhall DP

doi

10.1136/jcp.33.6.562

subject

Has Abstract

pub_date

1980-06-01 00:00:00

pages

562-5

issue

6

eissn

0021-9746

issn

1472-4146

journal_volume

33

pub_type

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