Abstract：
:Aflatoxin B(1) (AFB(1)) is commonly found in cereals and animal feeds and causes a significant threat to the food industry and animal production. Several microbial isolates with high AFB(1) transformation ability have been identified in our previous studies. The aim of this research was to characterize one of those isolates, Myxococcus fulvus ANSM068, and to explore its biotransformation mechanism. The bacterial isolate of M. fulvus ANSM068, isolated from deer feces, was able to transform AFB(1) by 80.7% in liquid VY/2 medium after incubation at 30 °C for 72 h. The supernatant of the bacterial culture was more effective in transforming AFB(1) as compared to the cells alone and the cell extract. The transformation activity was significantly reduced and eradicated after the culture supernatant was treated with proteinase K, proteinase K plus SDS and heating. Culture conditions, including nitrogen source, initial pH and incubation temperature were evaluated for an optimal AFB(1) transformation. Liquid chromatography mass spectrometry (LCMS) analyses showed that AFB(1) was transformed to a structurally different compound. Infrared analysis (IR) indicated that the lactone ring on the AFB(1) molecule was modified by the culture supernatant. Chromatographies on DEAE-Ion exchange and Sephadex-Molecular sieve and SDS-PAGE electrophoresis were used to determine active components from the culture supernatant, indicating that enzyme(s) were responsible for the AFB(1) biotransformation. This is the first report on AFB(1) transformation by a strain of myxobacteria through enzymatic reaction(s).
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Guan S,Zhao L,Ma Q,Zhou T,Wang N,Hu X,Ji Cdoi
10.3390/ijms11104063subject
Has Abstractpub_date
2010-10-20 00:00:00pages
4063-79issue
10issn
1422-0067journal_volume
11pub_type
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